JP6088710B2 - 単分子検出アッセイおよびその使用 - Google Patents

単分子検出アッセイおよびその使用 Download PDF

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JP6088710B2
JP6088710B2 JP2016536393A JP2016536393A JP6088710B2 JP 6088710 B2 JP6088710 B2 JP 6088710B2 JP 2016536393 A JP2016536393 A JP 2016536393A JP 2016536393 A JP2016536393 A JP 2016536393A JP 6088710 B2 JP6088710 B2 JP 6088710B2
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JP2016527918A5 (enExample
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フェール、エイドリアン・ニールセン
コリンズ、パトリック・ジェームズ
ハーシュレブ、ジル・リンドン
ジョーンズ、ハイウェル・ボーデン
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シンギュラー・バイオ・インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
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  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016536393A 2013-08-19 2014-08-19 単分子検出アッセイおよびその使用 Active JP6088710B2 (ja)

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US201361867559P 2013-08-19 2013-08-19
US201361867554P 2013-08-19 2013-08-19
US61/867,554 2013-08-19
US61/867,559 2013-08-19
PCT/US2014/051763 WO2015026873A1 (en) 2013-08-19 2014-08-19 Assays for single molecule detection and use thereof

Related Child Applications (1)

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JP2016527918A JP2016527918A (ja) 2016-09-15
JP2016527918A5 JP2016527918A5 (enExample) 2016-10-27
JP6088710B2 true JP6088710B2 (ja) 2017-03-01

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US (5) US9212394B2 (enExample)
EP (3) EP3036545B1 (enExample)
JP (2) JP6088710B2 (enExample)
CN (2) CN110283888B (enExample)
AU (2) AU2014308980C1 (enExample)
BR (1) BR112016003480B1 (enExample)
CA (1) CA2921628A1 (enExample)
ES (1) ES2739430T3 (enExample)
IL (3) IL285521B2 (enExample)
MX (2) MX371428B (enExample)
SG (1) SG11201601201VA (enExample)
WO (1) WO2015026873A1 (enExample)
ZA (1) ZA201601029B (enExample)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
US10648030B2 (en) 2012-01-13 2020-05-12 Affymetrix, Inc. Methods of determining the presence or absence of a plurality of target polynucleotides in a sample
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
WO2015026873A1 (en) * 2013-08-19 2015-02-26 Singular Bio, Inc. Assays for single molecule detection and use thereof
CN106460070B (zh) 2014-04-21 2021-10-08 纳特拉公司 检测染色体片段中的突变和倍性
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
EP3169813B1 (en) 2014-07-18 2019-06-12 The Chinese University Of Hong Kong Methylation pattern analysis of tissues in dna mixture
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
US11739371B2 (en) 2015-02-18 2023-08-29 Invitae Corporation Arrays for single molecule detection and use thereof
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
CN107532203B (zh) * 2015-03-06 2019-09-13 真固生物科技有限公司 重叠扩增子的选择性扩增
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CA2991401C (en) 2015-07-07 2023-12-05 Pillar Biosciences Inc. Method for reducing primer-dimer amplification
US11118216B2 (en) * 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
ES2913468T3 (es) 2016-04-15 2022-06-02 Natera Inc Métodos para la detección del cáncer de pulmón.
CN106053405B (zh) * 2016-05-10 2018-10-02 东南大学 一种基于单分子定位法的超分辨光学成像方法
CN109477095A (zh) * 2016-05-26 2019-03-15 卓异生物公司 用于单分子检测的阵列及其应用
WO2018045270A1 (en) * 2016-09-02 2018-03-08 Singular Bio, Inc. Systems and methods for single molecule quantification
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
WO2018099418A1 (en) 2016-11-30 2018-06-07 The Chinese University Of Hong Kong Analysis of cell-free dna in urine and other samples
US11093307B2 (en) * 2016-12-08 2021-08-17 Accenture Global Solutions Limited Platform for supporting multiple virtual agent applications
EP3595806A4 (en) * 2017-03-17 2021-06-16 Apton Biosystems, Inc. SEQUENCING AND HIGH RESOLUTION IMAGING
CN110945136A (zh) 2017-06-20 2020-03-31 威斯康星州立大学医学院 使用总无细胞dna评估移植并发症风险
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
JP7573443B2 (ja) 2018-04-14 2024-10-25 ナテラ， インコーポレイテッド 循環腫瘍ｄｎａの個別化された検出を用いる癌検出およびモニタリングの方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3591401A1 (de) 2018-07-06 2020-01-08 Euroimmun Medizinische Labordiagnostika AG Verfahren zum automatisierten detektieren von antikörpern in einer flüssigen biologischen probe unter verwendung eines antigen-chips und antigen-chip hierfür
US11931674B2 (en) 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
EP4074823B1 (en) * 2019-12-09 2024-12-25 Toppan Inc. Detection method
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
CN112419235B (zh) * 2020-10-22 2024-07-23 南京大学 一种精子活性检测系统及方法
CN112345766B (zh) * 2020-10-22 2024-02-02 中国工程物理研究院核物理与化学研究所 一种荧光-放射性联用的体外靶向筛选方法
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4092136B8 (en) 2021-05-20 2024-03-13 Sophia Genetics S.A. Capture probes and uses thereof
WO2023102502A2 (en) * 2021-12-01 2023-06-08 Siscapa Assay Technologies, Inc. Enriched peptide detection by single molecule sequencing
EP4581367A1 (en) * 2022-09-01 2025-07-09 The Johns Hopkins University Methods for measuring telomere length

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5256536A (en) 1990-11-09 1993-10-26 Syntex (U.S.A.) Inc. Nucleotide probe for Neisseria gonrrhoeae
WO1992010092A1 (en) 1990-12-06 1992-06-25 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
WO1992011390A1 (en) 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
WO1993017126A1 (en) 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5670330A (en) 1992-09-29 1997-09-23 Mcgill University Anti-tumor agent assay using PKR
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
EP0852004B1 (en) 1995-10-11 2011-01-19 Luminex Corporation Multiplexed analysis of clinical specimens
EP2574617B1 (en) * 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
JP4468488B2 (ja) * 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6706473B1 (en) 1996-12-06 2004-03-16 Nanogen, Inc. Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides
US6028189A (en) 1997-03-20 2000-02-22 University Of Washington Solvent for oligonucleotide synthesis and methods of use
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6284497B1 (en) 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
CA2355816C (en) 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20020086289A1 (en) 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6495320B1 (en) 1999-07-21 2002-12-17 Affymetrix, Inc. Even length proportional amplification of nucleic acids
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
DK1259643T3 (da) 2000-02-07 2009-02-23 Illumina Inc Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming
AU2001293366A1 (en) 2000-04-14 2001-10-30 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
WO2001092579A2 (en) * 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
US6635425B2 (en) 2001-01-05 2003-10-21 Molecular Staging, Inc. 5′-thio phosphate directed ligation of oligonucleotides and use in detection of single nucleotide polymorphisms
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US20020197630A1 (en) 2001-04-12 2002-12-26 Knapp Michael R. Systems and methods for high throughput genetic analysis
AU2003256587A1 (en) 2002-07-18 2004-02-09 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
WO2004027093A1 (en) * 2002-09-19 2004-04-01 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
CA2499360A1 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
CA2555962C (en) * 2003-02-26 2015-10-06 Callida Genomics, Inc. Random array dna analysis by hybridization
US7105024B2 (en) 2003-05-06 2006-09-12 Aesculap Ii, Inc. Artificial intervertebral disc
EP1602733A1 (en) 2004-06-02 2005-12-07 Keygene N.V. Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
WO2005026329A2 (en) * 2003-09-12 2005-03-24 Cornell Research Foundation, Inc. Methods for identifying target nucleic acid molecules
ATE435301T1 (de) 2003-10-16 2009-07-15 Sequenom Inc Nicht invasiver nachweis fötaler genetischer merkmale
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN101087890A (zh) * 2004-07-26 2007-12-12 帕拉列勒生物科学公司 多个基因组的同时分析
CN102010902B (zh) * 2005-03-18 2012-05-23 香港中文大学 产前诊断和监测的标记
CN1824786A (zh) * 2005-12-23 2006-08-30 上海生物芯片有限公司 基于连接的选择性扩增方法
US20080038733A1 (en) 2006-03-28 2008-02-14 Baylor College Of Medicine Screening for down syndrome
EP2029779A4 (en) * 2006-06-14 2010-01-20 Living Microsystems Inc HIGHLY PARALLEL SNP GENOTYPING UTILIZATION FOR FETAL DIAGNOSIS
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
JP5165933B2 (ja) * 2007-06-12 2013-03-21 日本碍子株式会社 標的核酸中の特定部分配列の検出方法及びアレイ
EP2176281A4 (en) 2007-07-11 2010-09-15 Univ Laval NUCLEIC ACID SEQUENCES AND COMBINATION THEREOF FOR SENSITIVE AMPLIFICATION AND SENSITIVE DETECTION OF BACTERIAL AND MIRACLE SEPSISPATHOGENESE
US20100297585A1 (en) 2008-01-29 2010-11-25 Uriel Yarovesky Process for making a dental restoration and resultant apparatus
JP5319148B2 (ja) * 2008-03-27 2013-10-16 日本碍子株式会社 標的核酸中の変異の検出方法及びアレイ
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9017971B2 (en) 2008-11-05 2015-04-28 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
US20100273164A1 (en) 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
EP2443457A4 (en) 2009-06-18 2012-10-31 Penn State Res Found METHODS, SYSTEMS AND KITS FOR DETECTING PROTEIN NUCLEIC ACID INTERACTIONS
JP5613160B2 (ja) * 2009-07-09 2014-10-22 日本碍子株式会社 ゲノムｄｎａ中の標的配列の検出又は解析方法
CN101864482A (zh) * 2010-02-10 2010-10-20 深圳出入境检验检疫局动植物检验检疫技术中心 Mlcr探针、二步反应模式和悬浮芯片检测用捕获探针
WO2011142836A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Assays for the detection of genotype, mutations, and/or aneuploidy
SG185723A1 (en) 2010-06-07 2013-01-30 Esoterix Genetic Lab Llc Enumeration of nucleic acids
US20120021919A1 (en) * 2010-07-23 2012-01-26 Thomas Scholl Identification of Differentially Represented Fetal or Maternal Genomic Regions and Uses Thereof
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20120077185A1 (en) 2010-08-06 2012-03-29 Tandem Diagnostics, Inc. Detection of genetic abnormalities and infectious disease
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20120190557A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
US20140342940A1 (en) * 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
WO2012021749A1 (en) * 2010-08-11 2012-02-16 Celula, Inc. Genotyping dna
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US8759036B2 (en) * 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
AU2012281242B2 (en) 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
CN104204228A (zh) 2012-02-14 2014-12-10 康奈尔大学 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法
US20130310262A1 (en) 2012-05-21 2013-11-21 Ariosa Diagnostics, Inc. Noninvasive detection of robertsonian translocations
US9206417B2 (en) 2012-07-19 2015-12-08 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
CN102936755B (zh) * 2012-10-19 2015-01-21 上海交通大学 一种单分子核酸芯片的制作方法
EP2929044B1 (en) 2012-12-07 2019-02-06 Invitae Corporation Multiplex nucleic acid detection methods
US20140242582A1 (en) 2013-02-28 2014-08-28 Ariosa Diagnostics, Inc. Detection of genetic abnormalities using ligation-based detection and digital pcr
EP2976435B1 (en) 2013-03-19 2017-10-25 Directed Genomics, LLC Enrichment of target sequences
JP6656733B2 (ja) 2013-08-05 2020-03-04 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
WO2015026873A1 (en) 2013-08-19 2015-02-26 Singular Bio, Inc. Assays for single molecule detection and use thereof
SG11201700765WA (en) 2014-08-01 2017-02-27 Ariosa Diagnostics Inc Detection of target nucleic acids using hybridization
WO2016070164A1 (en) 2014-11-01 2016-05-06 Singular Bio, Inc. Assays for single molecule detection and use thereof
US11739371B2 (en) 2015-02-18 2023-08-29 Invitae Corporation Arrays for single molecule detection and use thereof

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