JP2017060521A5 - - Google Patents
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- Publication number
- JP2017060521A5 JP2017060521A5 JP2016237574A JP2016237574A JP2017060521A5 JP 2017060521 A5 JP2017060521 A5 JP 2017060521A5 JP 2016237574 A JP2016237574 A JP 2016237574A JP 2016237574 A JP2016237574 A JP 2016237574A JP 2017060521 A5 JP2017060521 A5 JP 2017060521A5
- Authority
- JP
- Japan
- Prior art keywords
- reporter
- endocytosis
- signal
- detectable signal
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000012202 endocytosis Effects 0.000 claims description 73
- 210000004027 cell Anatomy 0.000 claims description 70
- 239000000758 substrate Substances 0.000 claims description 44
- 108060001084 Luciferase Proteins 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 37
- 102000037865 fusion proteins Human genes 0.000 claims description 26
- 108020001507 fusion proteins Proteins 0.000 claims description 26
- 230000004927 fusion Effects 0.000 claims description 24
- 210000000170 cell membrane Anatomy 0.000 claims description 22
- 239000012491 analyte Substances 0.000 claims description 20
- 210000001163 endosome Anatomy 0.000 claims description 18
- 210000001723 extracellular space Anatomy 0.000 claims description 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 18
- 238000012544 monitoring process Methods 0.000 claims description 14
- 108010001857 Cell Surface Receptors Proteins 0.000 claims description 13
- 229920001184 polypeptide Polymers 0.000 claims description 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 13
- 210000003093 intracellular space Anatomy 0.000 claims description 12
- 241000254173 Coleoptera Species 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 9
- 230000003993 interaction Effects 0.000 claims description 8
- 230000004071 biological effect Effects 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 3
- HBZBAMXERPYTFS-SECBINFHSA-N (4S)-2-(6,7-dihydro-5H-pyrrolo[3,2-f][1,3]benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid Chemical compound OC(=O)[C@H]1CSC(=N1)c1nc2cc3CCNc3cc2s1 HBZBAMXERPYTFS-SECBINFHSA-N 0.000 claims 4
- 230000032895 transmembrane transport Effects 0.000 claims 4
- 102000006240 membrane receptors Human genes 0.000 claims 2
- 241001443978 Oplophorus Species 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 description 11
- 102000000844 Cell Surface Receptors Human genes 0.000 description 10
- YHIPILPTUVMWQT-UHFFFAOYSA-N Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 description 9
- 108010076504 Protein Sorting Signals Proteins 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical group OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 6
- 230000028327 secretion Effects 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 3
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 3
- 108010053070 Glutathione Disulfide Proteins 0.000 description 3
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 3
- 102000018697 Membrane Proteins Human genes 0.000 description 3
- 108010052285 Membrane Proteins Proteins 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 102000004889 Interleukin-6 Human genes 0.000 description 2
- 230000005754 cellular signaling Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000018 receptor agonist Substances 0.000 description 2
- 229940044601 receptor agonist Drugs 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108090000331 Firefly luciferases Proteins 0.000 description 1
- 108010052090 Renilla Luciferases Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161563294P | 2011-11-23 | 2011-11-23 | |
| US61/563,294 | 2011-11-23 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014543569A Division JP6058688B2 (ja) | 2011-11-23 | 2012-11-21 | 膜貫通輸送を監視するための組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017060521A JP2017060521A (ja) | 2017-03-30 |
| JP2017060521A5 true JP2017060521A5 (enExample) | 2018-05-17 |
| JP6419139B2 JP6419139B2 (ja) | 2018-11-07 |
Family
ID=48470309
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014543569A Active JP6058688B2 (ja) | 2011-11-23 | 2012-11-21 | 膜貫通輸送を監視するための組成物および方法 |
| JP2016237574A Active JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014543569A Active JP6058688B2 (ja) | 2011-11-23 | 2012-11-21 | 膜貫通輸送を監視するための組成物および方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US9096889B2 (enExample) |
| EP (1) | EP2783218B1 (enExample) |
| JP (2) | JP6058688B2 (enExample) |
| WO (1) | WO2013078362A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6058688B2 (ja) | 2011-11-23 | 2017-01-11 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 膜貫通輸送を監視するための組成物および方法 |
| WO2014052653A1 (en) * | 2012-09-26 | 2014-04-03 | Promega Corporation | Real-time monitoring |
| JP6484987B2 (ja) * | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| JP6265020B2 (ja) | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0898680A (ja) * | 1995-07-21 | 1996-04-16 | Kikkoman Corp | ルシフェラーゼの製造方法 |
| US7853411B2 (en) * | 1997-02-27 | 2010-12-14 | Cellomics, Inc. | System for cell-based screening |
| GB9903767D0 (en) | 1999-02-18 | 1999-04-14 | Univ Glasgow | Receptor assay |
| EP1156103B1 (en) * | 2000-04-26 | 2010-08-11 | Chisso Corporation | Oplophorus luciferase |
| US20030013137A1 (en) | 2001-03-13 | 2003-01-16 | Barak Larry S. | Automated methods of detecting receptor activity |
| CA2596715A1 (en) | 2005-02-01 | 2006-08-10 | Medical Research Council | Screening method |
| JP5021273B2 (ja) * | 2006-05-18 | 2012-09-05 | キッコーマン株式会社 | 老化度の判断方法 |
| FR2934684B1 (fr) * | 2008-07-31 | 2012-11-16 | Cis Bio Int | Methode de detection de l'internalisation de proteines membranaires. |
| US8795977B2 (en) * | 2009-12-02 | 2014-08-05 | Conopco, Inc. | Method for screening a potential modulator compound of a taste receptor |
| JP6058688B2 (ja) | 2011-11-23 | 2017-01-11 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 膜貫通輸送を監視するための組成物および方法 |
-
2012
- 2012-11-21 JP JP2014543569A patent/JP6058688B2/ja active Active
- 2012-11-21 US US13/683,662 patent/US9096889B2/en active Active
- 2012-11-21 WO PCT/US2012/066325 patent/WO2013078362A1/en not_active Ceased
- 2012-11-21 EP EP12851936.0A patent/EP2783218B1/en active Active
-
2015
- 2015-07-15 US US14/800,352 patent/US9738921B2/en active Active
-
2016
- 2016-12-07 JP JP2016237574A patent/JP6419139B2/ja active Active
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