JP6058688B2 - 膜貫通輸送を監視するための組成物および方法 - Google Patents
膜貫通輸送を監視するための組成物および方法 Download PDFInfo
- Publication number
- JP6058688B2 JP6058688B2 JP2014543569A JP2014543569A JP6058688B2 JP 6058688 B2 JP6058688 B2 JP 6058688B2 JP 2014543569 A JP2014543569 A JP 2014543569A JP 2014543569 A JP2014543569 A JP 2014543569A JP 6058688 B2 JP6058688 B2 JP 6058688B2
- Authority
- JP
- Japan
- Prior art keywords
- reporter
- endocytosis
- cell surface
- signal
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
- G01N33/567—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds utilising isolate of tissue or organ as binding agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161563294P | 2011-11-23 | 2011-11-23 | |
| US61/563,294 | 2011-11-23 | ||
| PCT/US2012/066325 WO2013078362A1 (en) | 2011-11-23 | 2012-11-21 | Compositions and methods for monitoring transmembrane trafficking |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016237574A Division JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014533520A JP2014533520A (ja) | 2014-12-15 |
| JP2014533520A5 JP2014533520A5 (enExample) | 2016-03-03 |
| JP6058688B2 true JP6058688B2 (ja) | 2017-01-11 |
Family
ID=48470309
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014543569A Active JP6058688B2 (ja) | 2011-11-23 | 2012-11-21 | 膜貫通輸送を監視するための組成物および方法 |
| JP2016237574A Active JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016237574A Active JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US9096889B2 (enExample) |
| EP (1) | EP2783218B1 (enExample) |
| JP (2) | JP6058688B2 (enExample) |
| WO (1) | WO2013078362A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013078362A1 (en) | 2011-11-23 | 2013-05-30 | Promega Corporation | Compositions and methods for monitoring transmembrane trafficking |
| US20140099654A1 (en) * | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
| JP6484987B2 (ja) | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| JP6265020B2 (ja) | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0898680A (ja) * | 1995-07-21 | 1996-04-16 | Kikkoman Corp | ルシフェラーゼの製造方法 |
| US7853411B2 (en) * | 1997-02-27 | 2010-12-14 | Cellomics, Inc. | System for cell-based screening |
| GB9903767D0 (en) | 1999-02-18 | 1999-04-14 | Univ Glasgow | Receptor assay |
| DE60142761D1 (de) | 2000-04-26 | 2010-09-23 | Chisso Corp | Oplophorus Luciferase |
| US20030013137A1 (en) * | 2001-03-13 | 2003-01-16 | Barak Larry S. | Automated methods of detecting receptor activity |
| AU2006210703A1 (en) * | 2005-02-01 | 2006-08-10 | Medical Research Council | Screening method |
| JP5021273B2 (ja) * | 2006-05-18 | 2012-09-05 | キッコーマン株式会社 | 老化度の判断方法 |
| FR2934684B1 (fr) | 2008-07-31 | 2012-11-16 | Cis Bio Int | Methode de detection de l'internalisation de proteines membranaires. |
| WO2011067202A1 (en) * | 2009-12-02 | 2011-06-09 | Unilever Nv | Improved method for screening a potential modulator compound of a taste receptor |
| WO2013078362A1 (en) | 2011-11-23 | 2013-05-30 | Promega Corporation | Compositions and methods for monitoring transmembrane trafficking |
-
2012
- 2012-11-21 WO PCT/US2012/066325 patent/WO2013078362A1/en not_active Ceased
- 2012-11-21 US US13/683,662 patent/US9096889B2/en active Active
- 2012-11-21 EP EP12851936.0A patent/EP2783218B1/en active Active
- 2012-11-21 JP JP2014543569A patent/JP6058688B2/ja active Active
-
2015
- 2015-07-15 US US14/800,352 patent/US9738921B2/en active Active
-
2016
- 2016-12-07 JP JP2016237574A patent/JP6419139B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP6419139B2 (ja) | 2018-11-07 |
| EP2783218A1 (en) | 2014-10-01 |
| EP2783218A4 (en) | 2015-06-24 |
| EP2783218B1 (en) | 2017-01-04 |
| US9096889B2 (en) | 2015-08-04 |
| WO2013078362A1 (en) | 2013-05-30 |
| JP2014533520A (ja) | 2014-12-15 |
| US9738921B2 (en) | 2017-08-22 |
| US20150322482A1 (en) | 2015-11-12 |
| JP2017060521A (ja) | 2017-03-30 |
| US20130196357A1 (en) | 2013-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Donthamsetti et al. | Using Bioluminescence Resonance Energy Transfer (BRET) to characterize agonist‐induced arrestin recruitment to modified and unmodified G protein‐coupled receptors | |
| Hamdan et al. | High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based β-arrestin2 recruitment assay | |
| Klewe et al. | Recruitment of β-arrestin2 to the dopamine D2 receptor: insights into anti-psychotic and anti-parkinsonian drug receptor signaling | |
| JP4955391B2 (ja) | Gタンパク質共役受容体およびそのシグナル伝達経路の断片相補アッセイ | |
| JP6419139B2 (ja) | 膜貫通輸送を監視するための組成物および方法 | |
| EP3194960B1 (en) | Biosensors for monitoring biomolecule localization and trafficking in cells | |
| DiBerto et al. | Agonist and antagonist TRUPATH assays for G protein-coupled receptors | |
| US20200363424A1 (en) | Complex bret technique for measuring biological interactions | |
| Daly et al. | β-Arrestin-dependent and-independent endosomal G protein activation by the vasopressin type 2 receptor | |
| Milligan et al. | G protein-coupled receptor fusion proteins in drug discovery | |
| Hanson et al. | A homogeneous fluorescent live-cell assay for measuring 7-transmembrane receptor activity and agonist functional selectivity through beta-arrestin recruitment | |
| US7604959B2 (en) | Cell-based assays employing voltage and calcium dyes | |
| Xiao et al. | High throughput screening for orphan and liganded GPCRs | |
| Eglen | An overview of high throughput screening at G protein coupled receptors | |
| CA2607015A1 (en) | Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same | |
| EP2099925B1 (en) | Detection of sub-cellular compartment localization of a cell-surface receptor to an endosome using a reduced affinity enzyme complementation reporter system | |
| US20070105160A1 (en) | Detection of intracellular enzyme complex | |
| Wang et al. | High throughput screening (HTS) in identification new ligands and drugable targets of G protein-coupled receptors (GPCRs) | |
| TW200413722A (en) | Methods of identifying reduced internalization transmembrane receptor agonists | |
| US20090113563A1 (en) | Probe for detecting nuclear receptor agonist or antagonist and method for screening agonist or antagonist to nuclear receptor with the use of the same | |
| Mancini et al. | Exploring the Technology Landscape of 7TMR Drug Signaling Profiling | |
| Parichatikanond et al. | BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation | |
| WO2024044308A2 (en) | Materials and methods for detecting biased signaling of g-protein coupled receptors | |
| Chen et al. | Application of large-scale transient transfection to cell-based functional assays for ion channels and GPCRs | |
| WO2024151471A1 (en) | High-throughput assay based on ligand-biased structural dynamics response |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151124 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20151124 |
|
| A524 | Written submission of copy of amendment under article 19 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A524 Effective date: 20160114 |
|
| A871 | Explanation of circumstances concerning accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A871 Effective date: 20160114 |
|
| A975 | Report on accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A971005 Effective date: 20160202 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160307 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160607 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160906 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20161107 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20161207 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6058688 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |