JP2014533520A5 - - Google Patents
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- Publication number
- JP2014533520A5 JP2014533520A5 JP2014543569A JP2014543569A JP2014533520A5 JP 2014533520 A5 JP2014533520 A5 JP 2014533520A5 JP 2014543569 A JP2014543569 A JP 2014543569A JP 2014543569 A JP2014543569 A JP 2014543569A JP 2014533520 A5 JP2014533520 A5 JP 2014533520A5
- Authority
- JP
- Japan
- Prior art keywords
- reporter
- endocytosis
- signal
- cell
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 106
- 210000004027 cell Anatomy 0.000 claims description 84
- 230000012202 endocytosis Effects 0.000 claims description 84
- 239000000758 substrate Substances 0.000 claims description 62
- 108060001084 Luciferase Proteins 0.000 claims description 42
- 230000004927 fusion Effects 0.000 claims description 33
- 210000000170 cell membrane Anatomy 0.000 claims description 30
- 210000001723 extracellular space Anatomy 0.000 claims description 28
- 239000012491 analyte Substances 0.000 claims description 27
- 210000001163 endosome Anatomy 0.000 claims description 25
- 108020001507 fusion proteins Proteins 0.000 claims description 23
- 102000037865 fusion proteins Human genes 0.000 claims description 23
- YHIPILPTUVMWQT-UHFFFAOYSA-N Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 claims description 21
- 210000003093 intracellular space Anatomy 0.000 claims description 19
- 241000254173 Coleoptera Species 0.000 claims description 14
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical group OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 230000001939 inductive effect Effects 0.000 claims description 11
- 108010001857 Cell Surface Receptors Proteins 0.000 claims description 8
- 102000006240 membrane receptors Human genes 0.000 claims description 8
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims description 7
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims description 7
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims description 7
- 230000007306 turnover Effects 0.000 claims description 7
- 230000003993 interaction Effects 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 18
- 229920001184 polypeptide Polymers 0.000 description 16
- 102000004196 processed proteins & peptides Human genes 0.000 description 16
- 238000012544 monitoring process Methods 0.000 description 14
- 108090000623 proteins and genes Proteins 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 10
- 239000005089 Luciferase Substances 0.000 description 8
- 239000012528 membrane Substances 0.000 description 7
- 230000032895 transmembrane transport Effects 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 230000005754 cellular signaling Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 239000000018 receptor agonist Substances 0.000 description 2
- 229940044601 receptor agonist Drugs 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 108090000331 Firefly luciferases Proteins 0.000 description 1
- 241000254064 Photinus pyralis Species 0.000 description 1
- 108010052090 Renilla Luciferases Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000006635 beta-lactamase Human genes 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161563294P | 2011-11-23 | 2011-11-23 | |
| US61/563,294 | 2011-11-23 | ||
| PCT/US2012/066325 WO2013078362A1 (en) | 2011-11-23 | 2012-11-21 | Compositions and methods for monitoring transmembrane trafficking |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016237574A Division JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014533520A JP2014533520A (ja) | 2014-12-15 |
| JP2014533520A5 true JP2014533520A5 (enExample) | 2016-03-03 |
| JP6058688B2 JP6058688B2 (ja) | 2017-01-11 |
Family
ID=48470309
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014543569A Active JP6058688B2 (ja) | 2011-11-23 | 2012-11-21 | 膜貫通輸送を監視するための組成物および方法 |
| JP2016237574A Active JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016237574A Active JP6419139B2 (ja) | 2011-11-23 | 2016-12-07 | 膜貫通輸送を監視するための組成物および方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US9096889B2 (enExample) |
| EP (1) | EP2783218B1 (enExample) |
| JP (2) | JP6058688B2 (enExample) |
| WO (1) | WO2013078362A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6058688B2 (ja) | 2011-11-23 | 2017-01-11 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 膜貫通輸送を監視するための組成物および方法 |
| US20140099654A1 (en) * | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
| JP6484987B2 (ja) | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| JP6265020B2 (ja) | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0898680A (ja) * | 1995-07-21 | 1996-04-16 | Kikkoman Corp | ルシフェラーゼの製造方法 |
| US7853411B2 (en) * | 1997-02-27 | 2010-12-14 | Cellomics, Inc. | System for cell-based screening |
| GB9903767D0 (en) * | 1999-02-18 | 1999-04-14 | Univ Glasgow | Receptor assay |
| DE60142761D1 (de) * | 2000-04-26 | 2010-09-23 | Chisso Corp | Oplophorus Luciferase |
| US20030013137A1 (en) * | 2001-03-13 | 2003-01-16 | Barak Larry S. | Automated methods of detecting receptor activity |
| WO2006082385A1 (en) | 2005-02-01 | 2006-08-10 | Medical Research Council | Screening method |
| JP5021273B2 (ja) * | 2006-05-18 | 2012-09-05 | キッコーマン株式会社 | 老化度の判断方法 |
| FR2934684B1 (fr) | 2008-07-31 | 2012-11-16 | Cis Bio Int | Methode de detection de l'internalisation de proteines membranaires. |
| WO2011067202A1 (en) * | 2009-12-02 | 2011-06-09 | Unilever Nv | Improved method for screening a potential modulator compound of a taste receptor |
| JP6058688B2 (ja) | 2011-11-23 | 2017-01-11 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 膜貫通輸送を監視するための組成物および方法 |
-
2012
- 2012-11-21 JP JP2014543569A patent/JP6058688B2/ja active Active
- 2012-11-21 EP EP12851936.0A patent/EP2783218B1/en active Active
- 2012-11-21 US US13/683,662 patent/US9096889B2/en active Active
- 2012-11-21 WO PCT/US2012/066325 patent/WO2013078362A1/en not_active Ceased
-
2015
- 2015-07-15 US US14/800,352 patent/US9738921B2/en active Active
-
2016
- 2016-12-07 JP JP2016237574A patent/JP6419139B2/ja active Active
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