JP2016539640A - 化学反応の光学制御 - Google Patents
化学反応の光学制御 Download PDFInfo
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Abstract
Description
‐基板の第一の面上に少なくとも一つの分子を結合するための基板。当該第一の面は反応チャンバの壁(境界)である。基板は特にワイヤグリッドであり得る。
‐光化学切断反応を光学的に誘導するために基板へ光を向けるように構成される光学構成。その効果のために、この光は以下"切断光"とよばれる。
‐反応チャンバにおいて試薬流体を循環させるための循環構成。
‐分子が基板の第一の面上に結合した基板を設けるステップ。当該第一の面は反応チャンバの壁である。
‐光学構成によって、切断波長λCLの切断光、好適にはUV光で基板を照射し、それにより光化学切断反応を光学的に誘導するステップ。
‐反応チャンバにおいて、好適には基板の第一の面に沿って、試薬流体を循環させるステップ。
Claims (15)
- 試薬流体を有する反応チャンバにおいて化学反応を光学的に制御するための装置であって、
基板の第一の面上に少なくとも一つの分子を結合するための当該基板であって、当該第一の面は前記反応チャンバの壁である、基板と、
光化学切断反応を光学的に誘導するために前記基板へ切断光を向けるように構成される光学構成と、
前記反応チャンバにおいて前記試薬流体を循環させるための循環構成と
を有し、前記基板がワイヤグリッドとして構成される、装置。 - 前記試薬流体が前記基板の第一の面に沿って循環される、請求項1に記載の装置。
- 前記循環構成が、前記試薬流体を能動的にポンプするためのポンプ素子及び/又は前記試薬流体を冷却するための冷却素子を有する、請求項1に記載の装置。
- 前記循環構成が少なくとも一つの空気圧駆動アクチュエータを有する、請求項1に記載の装置。
- 前記循環構成が前記試薬流体の循環を前記切断光の照射と同期させるように構成される、請求項1に記載の装置。
- 前記化学反応が核酸シークエンシングを、特に合成によって核酸のシーケンスを決定する反復段階反応を有する、請求項1に記載の装置。
- 前記光学構成が、前記基板の第一の面上に結合した分子に組み込まれた第一のヌクレオチドの蛍光ラベルを励起するために少なくとも第一の励起波長λEX1の励起光を前記基板へ向けるように構成され、
前記光学構成がさらに、前記結合した分子に組み込まれた第一のヌクレオチドの蛍光ラベルによって発せられる蛍光を受信及び検出するように構成される、
請求項1に記載の装置。 - 前記切断光、好適にはUV光が、前記組み込まれた第一のヌクレオチドからブロッキング部分と前記蛍光ラベルを切断する光化学切断反応を前記組み込まれた第一のヌクレオチドにおいて光学的に誘導する切断波長λCLを持つ、請求項7に記載の装置。
- 前記基板が前記励起光を閉じ込めるように構成され、前記基板の第一の面において前記励起光のエバネセント波をもたらすように構成され、及び/又は、
前記基板が前記切断光を閉じ込めるように構成され、前記基板の第一の面において前記切断光のエバネセント波をもたらすように構成される、
請求項1又は7に記載の装置。 - 前記基板の第一の面に結合した分子と、
複数のヌクレオチド及び酵素を伴う溶液と
をさらに有し、
前記ヌクレオチドがそれぞれブロッキング部分を有し、
前記ブロッキング部分は、各ヌクレオチドが前記第一の面に結合した分子に組み込まれるときに前記酵素の合成活性をブロックするように構成される、
請求項8に記載の装置。 - 試薬流体を有する反応チャンバにおいて化学反応を光学的に制御するための方法であって、
基板を設けるステップであって、当該基板の第一の面上に分子が結合し、当該第一の面は前記反応チャンバの壁である、ステップと、
光学構成によって、切断波長λCLの切断光、好適にはUV光で前記基板を照射し、それにより光化学切断反応を光学的に誘導するステップと、
前記反応チャンバにおいて前記試薬流体を循環させるステップと
を有する方法。 - 前記基板の第一の面に沿って前記反応チャンバにおいて前記試薬流体を循環させるステップ、並びに/或いは、前記試薬流体の能動的ポンプ及び/又は冷却ステップをさらに有する、請求項11に記載の方法。
- 前記試薬流体の循環が前記切断光の照射と同期される、請求項11に記載の方法。
- 前記切断光の強度が約0.1mW/cm2よりも大きい、請求項11に記載の方法。
- 光学構成によって、少なくとも第一の励起波長λEX1の励起光で前記基板を照射し、それにより前記基板上に結合した分子に組み込まれる第一のヌクレオチドの蛍光ラベルを光学的に励起するステップと、
前記基板によって前記励起光を閉じ込め、それにより前記基板の第一の面において前記基板により前記励起光のエバネセント波をもたらすステップと、
前記光学構成によって、前記組み込まれた第一のヌクレオチドの励起された蛍光ラベルの蛍光を受信及び検出するステップと、
前記切断光で前記基板を照射し、それにより前記組み込まれた第一のヌクレオチドにおいて光化学切断反応を光学的に誘導するステップと、
前記基板によって前記切断波長λCLの切断光を閉じ込め、それにより前記基板の第一の面において前記基板によって前記切断光のエバネセント波をもたらすステップと
をさらに有する、請求項11に記載の方法。
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PCT/EP2014/075067 WO2015078755A1 (en) | 2013-11-29 | 2014-11-20 | Optical controlling of a chemical reaction |
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WO2013105025A1 (en) * | 2012-01-13 | 2013-07-18 | Koninklijke Philips N.V. | Dna sequencing with reagent recycling on wiregrid |
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DE19844931C1 (de) * | 1998-09-30 | 2000-06-15 | Stefan Seeger | Verfahren zur DNS- oder RNS-Sequenzierung |
US6403313B1 (en) * | 1999-12-21 | 2002-06-11 | Ingeneus Corporation | Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators |
JP2003522963A (ja) * | 2000-02-18 | 2003-07-29 | アクララ バイオサイエンシーズ, インコーポレイテッド | 複数部位反応デバイスおよび方法 |
WO2002081729A2 (en) | 2001-04-06 | 2002-10-17 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
WO2003008943A1 (en) * | 2001-07-19 | 2003-01-30 | Tufts University | Optical array device and methods of use thereof for screening, analysis and manipulation of particles |
US20060257878A1 (en) * | 2003-07-11 | 2006-11-16 | Taiyo Yuden Co., Ltd. | Nucleic acid amplifier and method of nucleic acid amplification |
DE102004038359A1 (de) * | 2004-08-06 | 2006-03-16 | Rudolf Rigler | Paralleles Hochdurchsatz-Einzelmolekül-Sequenzierungsverfahren |
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WO2007072418A2 (en) * | 2005-12-22 | 2007-06-28 | Koninklijke Philips Electronics N.V. | Increasing energy density in sub-wavelength wire-grids |
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WO2009158451A1 (en) | 2008-06-25 | 2009-12-30 | Real-Time Genomics, Llc | Method and apparatus for melting curve analysis of nucleic acids in microarray format |
CN101831384A (zh) * | 2009-10-30 | 2010-09-15 | 中国人民解放军第三军医大学第一附属医院 | 生物芯片反应装置及利用该装置检测靶分子的方法 |
KR20230074639A (ko) * | 2013-08-28 | 2023-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
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- 2014-11-20 CN CN202110647168.1A patent/CN113390839A/zh active Pending
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- 2014-11-20 CN CN201480064934.XA patent/CN105792924A/zh active Pending
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WO2000053805A1 (en) * | 1999-03-10 | 2000-09-14 | Asm Scientific, Inc. | A method for direct nucleic acid sequencing |
WO2011116120A2 (en) * | 2010-03-16 | 2011-09-22 | Life Technologies Corporation | Method and apparatus for addressable flow cells in single molecule sequencing |
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Also Published As
Publication number | Publication date |
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EP3074120A1 (en) | 2016-10-05 |
WO2015078755A1 (en) | 2015-06-04 |
JP6502349B2 (ja) | 2019-04-17 |
CN113390839A (zh) | 2021-09-14 |
CN105792924A (zh) | 2016-07-20 |
BR112016011764A2 (pt) | 2017-08-08 |
EP3074120B1 (en) | 2019-03-13 |
BR112016011764B1 (pt) | 2021-06-22 |
US20170037468A1 (en) | 2017-02-09 |
RU2675502C1 (ru) | 2018-12-19 |
US10619202B2 (en) | 2020-04-14 |
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