JP2016536983A5 - - Google Patents

Download PDF

Info

Publication number
JP2016536983A5
JP2016536983A5 JP2016525617A JP2016525617A JP2016536983A5 JP 2016536983 A5 JP2016536983 A5 JP 2016536983A5 JP 2016525617 A JP2016525617 A JP 2016525617A JP 2016525617 A JP2016525617 A JP 2016525617A JP 2016536983 A5 JP2016536983 A5 JP 2016536983A5
Authority
JP
Japan
Prior art keywords
region
microfluidic
channel
con
enclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2016525617A
Other languages
English (en)
Japanese (ja)
Other versions
JP6557658B2 (ja
JP2016536983A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from US14/520,568 external-priority patent/US10010882B2/en
Publication of JP2016536983A publication Critical patent/JP2016536983A/ja
Publication of JP2016536983A5 publication Critical patent/JP2016536983A5/ja
Priority to JP2019129854A priority Critical patent/JP6944490B2/ja
Application granted granted Critical
Publication of JP6557658B2 publication Critical patent/JP6557658B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2016525617A 2013-10-22 2014-10-22 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法 Active JP6557658B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019129854A JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US61/996,969 2013-10-22
US201462058658P 2014-10-01 2014-10-01
US62/058,658 2014-10-01
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US14/520,568 2014-10-22
PCT/US2014/061837 WO2015061497A1 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019129854A Division JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Publications (3)

Publication Number Publication Date
JP2016536983A JP2016536983A (ja) 2016-12-01
JP2016536983A5 true JP2016536983A5 (https=) 2017-11-30
JP6557658B2 JP6557658B2 (ja) 2019-08-07

Family

ID=52993522

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2016525617A Active JP6557658B2 (ja) 2013-10-22 2014-10-22 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2019129854A Active JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2021147377A Active JP7333365B2 (ja) 2013-10-22 2021-09-10 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2023091448A Pending JP2023115034A (ja) 2013-10-22 2023-06-02 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2019129854A Active JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2021147377A Active JP7333365B2 (ja) 2013-10-22 2021-09-10 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2023091448A Pending JP2023115034A (ja) 2013-10-22 2023-06-02 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Country Status (9)

Country Link
US (4) US10010882B2 (https=)
EP (3) EP3760703A1 (https=)
JP (4) JP6557658B2 (https=)
KR (4) KR102113377B1 (https=)
AU (3) AU2014340089B2 (https=)
CA (2) CA2927701C (https=)
IL (3) IL297654A (https=)
SG (2) SG11201602629RA (https=)
WO (1) WO2015061497A1 (https=)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8535241B2 (en) 2011-10-13 2013-09-17 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
JP6200948B2 (ja) 2012-05-25 2017-09-20 ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル マイクロ流体デバイス
US9060724B2 (en) 2012-05-30 2015-06-23 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
US9022950B2 (en) 2012-05-30 2015-05-05 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
WO2014022275A1 (en) 2012-08-01 2014-02-06 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
WO2014058945A1 (en) 2012-10-11 2014-04-17 Bullington Gregory J Systems and methods for delivering a fluid to a patient with reduced contamination
CN109171766A (zh) 2012-11-30 2019-01-11 木兰医药技术股份有限公司 体液隔绝装置和使用体液隔绝装置隔绝体液的方法
IL303591B2 (en) 2012-12-04 2025-11-01 Magnolia Medical Technologies Inc Sterile bodily-fluid collection device and methods
EP3760703A1 (en) * 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
WO2015188171A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
WO2016090295A1 (en) 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground
DK3229958T3 (da) 2014-12-08 2020-11-30 Berkeley Lights Inc Mikrofluidanordning, der omfatter laterale/vertikale transistorstrukturer, samt fremgangsmåde til fremstilling og anvendelse heraf
EP3229961B1 (en) * 2014-12-08 2019-11-13 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof
CN107223208B (zh) 2014-12-09 2021-04-09 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
EP3230718B1 (en) 2014-12-09 2022-03-02 Berkeley Lights, Inc. Automated detection of assay-positive areas or of analyte quantities in microfluidic devices
TWI700125B (zh) 2014-12-10 2020-08-01 美商柏克萊燈光有限公司 用於操作電氣動力裝置之系統
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
CN107810059B (zh) 2015-04-22 2021-03-23 伯克利之光生命科技公司 在微流体装置上冷冻和存档细胞
SG11201708429WA (en) * 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
TWI712686B (zh) * 2015-04-22 2020-12-11 美商柏克萊燈光有限公司 用於微流體裝置之培養站
US10101250B2 (en) 2015-04-22 2018-10-16 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
EP3313573A2 (en) * 2015-07-22 2018-05-02 The University of North Carolina at Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CN108495712A (zh) * 2015-11-23 2018-09-04 伯克利之光生命科技公司 原位生成的微流体隔离结构、试剂盒及其使用方法
US10705082B2 (en) * 2015-12-08 2020-07-07 Berkeley Lights, Inc. In situ-generated microfluidic assay structures, related kits, and methods of use thereof
CA3009073C (en) * 2015-12-30 2024-11-12 Berkeley Lights, Inc. MICROFLUID DEVICES FOR OPTICALLY CONTROLLED CONVECTION AND MOVEMENT, KITS AND ASSOCIATED PROCESSES
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis
EP3889176A1 (en) * 2016-01-15 2021-10-06 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
CN109196094A (zh) * 2016-03-17 2019-01-11 伯克利之光生命科技公司 微流体装置中t淋巴细胞的选择和克隆
JP7019590B2 (ja) 2016-03-31 2022-02-15 バークレー ライツ,インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
EP4684881A3 (en) * 2016-04-15 2026-03-18 Bruker Cellular Analysis, Inc. Methods, systems, computer program and non-transitory computer-readable medium for in-pen assays
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
CN115678773A (zh) 2016-05-26 2023-02-03 伯克利之光生命科技公司 共价修饰的表面、试剂盒及制备方法和用途
AU2017298545B2 (en) * 2016-07-21 2022-10-27 Berkeley Lights, Inc. Sorting of T lymphocytes in a microfluidic device
CA3038535A1 (en) 2016-10-01 2018-04-05 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
CN114755412A (zh) * 2016-10-23 2022-07-15 伯克利之光生命科技公司 筛选b细胞淋巴细胞的方法
CA3045333A1 (en) 2016-12-01 2018-06-07 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
AU2017368267B2 (en) 2016-12-01 2022-12-15 Berkeley Lights, Inc. Apparatuses, systems and methods for imaging micro-objects
US11473081B2 (en) 2016-12-12 2022-10-18 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
CN110546495B (zh) * 2016-12-30 2022-11-01 加利福尼亚州立大学董事会 用于基因组编辑t细胞的选择和传代的方法
JP7208902B2 (ja) 2016-12-30 2023-01-19 エクセラ・バイオサイエンシーズ・インコーポレイテッド マルチステージサンプル回収システム
JP2020510417A (ja) 2017-02-17 2020-04-09 コニク インコーポレイテッド 検出のためのシステム
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
JP7273807B2 (ja) 2017-06-09 2023-05-15 マグノリア メディカル テクノロジーズ,インコーポレイテッド 流体制御デバイス及びそれを使用する方法
TWI832820B (zh) 2017-07-21 2024-02-21 美商伯克利之光生命科技公司 抗原呈現合成表面、共價功能化表面、經活化之t細胞及其用途
EP3721209B1 (en) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Methods for in-pen assays
WO2020068174A2 (en) 2018-05-18 2020-04-02 The University Of North Carolina At Chapel Hill Compositions, devices, and methods for improving a surface property of a substrate
JP7526100B2 (ja) * 2018-05-31 2024-07-31 バークレー ライツ,インコーポレイテッド マイクロ流体デバイスによる微小物体の自動検出及び特徴付け
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
EP3849705A4 (en) * 2018-09-14 2022-06-01 Berkeley Lights, Inc. Methods for assaying binding affinity
US11993766B2 (en) 2018-09-21 2024-05-28 Bruker Cellular Analysis, Inc. Functionalized well plate, methods of preparation and use thereof
WO2020077274A1 (en) 2018-10-11 2020-04-16 Berkeley Lights, Inc. Systems and methods for identification of optimized protein production and kits therefor
KR20210078526A (ko) 2018-10-18 2021-06-28 버클리 라잇츠, 인크. 원 항원 제시 합성 표면, 활성화된 t 세포, 및 이들의 용도
CN113366312A (zh) * 2018-11-01 2021-09-07 伯克利之光生命科技公司 在微流体环境中测定生物细胞的方法
SG11202104544WA (en) 2018-11-19 2021-06-29 Berkeley Lights Inc Microfluidic device with programmable switching elements
EP3890876B1 (en) 2018-12-06 2024-05-01 Xcella Biosciences, Inc. Lateral loading of microcapillary arrays
WO2020163744A1 (en) 2019-02-08 2020-08-13 Magnolia Medical Technologies, Inc. Devices and methods for bodily fluid collection and distribution
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
IT201900002777A1 (it) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa Metodo e sistema microfluidico per l'isolamento di particelle
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
AU2020385015A1 (en) 2019-11-17 2022-06-09 Berkeley Lights, Inc. Systems and methods for analyses of biological samples
KR20220166788A (ko) 2020-03-09 2022-12-19 버클리 라잇츠, 인크. 펜내 분석을 위한 방법, 시스템 및 키트
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
WO2022051570A1 (en) 2020-09-07 2022-03-10 Berkeley Lights, Inc. Methods of assaying a biological cell

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255599C (en) 1996-04-25 2006-09-05 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1106244A3 (en) * 1999-03-03 2001-11-21 Symyx Technologies, Inc. Chemical processing microsystems and controlling reaction conditions in same
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
CA2521171C (en) 2003-04-03 2013-05-28 Fluidigm Corp. Microfluidic devices and methods of using same
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
US7425253B2 (en) 2004-01-29 2008-09-16 Massachusetts Institute Of Technology Microscale sorting cytometer
WO2005080606A1 (en) 2004-02-18 2005-09-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
US20070240495A1 (en) * 2004-05-25 2007-10-18 Shuzo Hirahara Microfluidic Device and Analyzing/Sorting Apparatus Using The Same
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
WO2007024701A2 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
CN101548004A (zh) 2005-08-19 2009-09-30 加利福尼亚大学董事会 用于诊断学和细胞分析的微流体方法
WO2007102839A2 (en) 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
US7964078B2 (en) * 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
AU2007265628B2 (en) 2006-06-23 2012-12-06 Revvity Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
US20080302732A1 (en) 2007-05-24 2008-12-11 Hyongsok Soh Integrated fluidics devices with magnetic sorting
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
JP2011516867A (ja) 2008-04-03 2011-05-26 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 細胞、小胞、ナノ粒子およびバイオマーカーを分離および単離するためのエキソビボの多次元システム
US20110117634A1 (en) 2008-04-21 2011-05-19 Asaf Halamish Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
JP5623399B2 (ja) 2008-07-28 2014-11-12 アギア システムズ エルエルシーAgere Systems LLC 変量補償浮上量測定システムおよび方法
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
WO2010115167A2 (en) 2009-04-03 2010-10-07 The Regents Of The University Of California Methods and devices for sorting cells and other biological particulates
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
CN102472697A (zh) 2009-07-06 2012-05-23 索尼公司 微流体装置
US20110003330A1 (en) 2009-07-06 2011-01-06 Durack Gary P Microfluidic device
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
WO2011149032A1 (ja) 2010-05-26 2011-12-01 東ソー株式会社 生体試料固定装置
CN101947124B (zh) 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
CN103261436B (zh) 2010-09-14 2015-03-25 加利福尼亚大学董事会 利用微流体截留涡流从异质溶液中分离细胞的方法
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
EP2646798B1 (en) * 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
US10571475B2 (en) * 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
US9902990B2 (en) 2011-05-27 2018-02-27 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
CN103998394B (zh) 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US10626435B2 (en) * 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
US9144806B2 (en) 2012-07-04 2015-09-29 Industrial Technology Research Institute Optically-induced dielectrophoresis device
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
KR102257305B1 (ko) 2013-03-28 2021-05-28 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3783094B1 (en) 2013-10-22 2023-10-11 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3760703A1 (en) 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
DK3229958T3 (da) 2014-12-08 2020-11-30 Berkeley Lights Inc Mikrofluidanordning, der omfatter laterale/vertikale transistorstrukturer, samt fremgangsmåde til fremstilling og anvendelse heraf
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture

Similar Documents

Publication Publication Date Title
JP2016536983A5 (https=)
ES2390800T3 (es) Aparatos y métodos para manipular gotitas en una placa de circuito impreso
AU2015249293B2 (en) DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US10376886B2 (en) Micro-fluidic devices for assaying biological activity
CN108796038B (zh) 一种核酸一体化检测方法及检测试剂管
EP3783094B1 (en) Micro-fluidic devices for assaying biological activity
Voldman et al. A microfabrication-based dynamic array cytometer
Yan et al. Isolating plasma from blood using a dielectrophoresis-active hydrophoretic device
Tong et al. Combining sensors and actuators with electrowetting-on-dielectric (EWOD): Advanced digital microfluidic systems for biomedical applications
EP3060645B1 (en) Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US20170043343A1 (en) Dep force control and electrowetting control in different sections of the same microfluidic apparatus
US11213824B2 (en) Microfluidic device and methods
EP3473699B1 (en) Exporting a selected group of micro-objects from a micro-fluidic device
US10308977B2 (en) Device and method for processing a biological sample and analysis system for analyzing a biological specimen
US20140348706A1 (en) Microfluidic Device and Microfluidic System
Vaidyanathan et al. Microfluidics for cell sorting and single cell analysis from whole blood
EP2798055A2 (en) Two dimensional nanofluidic ccd arrays for manipulation of charged molecules in solution
CN104789443B (zh) 一种细胞定位单元、阵列、器件及其形成方法
US20240189821A1 (en) Method and Device for Trapping at Least One Nucleated Cell Using at Least One Electrode for a Microfluidic Device
Kuroda et al. Microfluidic sedimentation system for separation of plasma from whole blood
EP3107656A2 (en) A micro-droplet array for multiple screening of a sample
JP2019187448A (ja) 微小物体の選択された群を微少流体デバイスから搬出すること
Chen et al. High-efficiency rare cell identification on a high-density self-assembled cell arrangement chip
JP6238397B2 (ja) 細胞空間分画装置および微細構造刃
Wang et al. Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip