JP2016512045A5 - - Google Patents
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- Publication number
- JP2016512045A5 JP2016512045A5 JP2016502626A JP2016502626A JP2016512045A5 JP 2016512045 A5 JP2016512045 A5 JP 2016512045A5 JP 2016502626 A JP2016502626 A JP 2016502626A JP 2016502626 A JP2016502626 A JP 2016502626A JP 2016512045 A5 JP2016512045 A5 JP 2016512045A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- amplification
- solid support
- str
- eluted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 16
- 108020004707 nucleic acids Proteins 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 108091092878 Microsatellite Proteins 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 239000007787 solid Substances 0.000 claims 4
- 239000000243 solution Substances 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 229920000858 Cyclodextrin Polymers 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 108091007412 Piwi-interacting RNA Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 238000010195 expression analysis Methods 0.000 claims 1
- 230000014509 gene expression Effects 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000006386 neutralization reaction Methods 0.000 claims 1
- -1 rRNA Proteins 0.000 claims 1
- 239000011535 reaction buffer Substances 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 239000003161 ribonuclease inhibitor Substances 0.000 claims 1
- 239000004055 small Interfering RNA Substances 0.000 claims 1
- 229940083575 sodium dodecyl sulfate Drugs 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
- 229940116269 uric acid Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361788068P | 2013-03-15 | 2013-03-15 | |
| US61/788,068 | 2013-03-15 | ||
| PCT/US2014/027790 WO2014143714A2 (en) | 2013-03-15 | 2014-03-14 | Methods for one step nucleic acid amplification of non-eluted samples |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016512045A JP2016512045A (ja) | 2016-04-25 |
| JP2016512045A5 true JP2016512045A5 (enExample) | 2017-04-13 |
| JP6559647B2 JP6559647B2 (ja) | 2019-08-14 |
Family
ID=50733326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016502626A Active JP6559647B2 (ja) | 2013-03-15 | 2014-03-14 | 非溶出試料のワンステップ核酸増幅方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10053686B2 (enExample) |
| EP (1) | EP2971109B1 (enExample) |
| JP (1) | JP6559647B2 (enExample) |
| WO (1) | WO2014143714A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014072354A1 (en) * | 2012-11-09 | 2014-05-15 | Ge Healthcare Uk Limited | Methods for one step nucleic acid amplification of non-eluted samples |
| US20150111214A1 (en) * | 2013-10-17 | 2015-04-23 | Life Technologies Corporation | Direct quantification of unprocessed nucleic acid samples |
| US9212388B1 (en) * | 2014-06-30 | 2015-12-15 | Life Technologies Corporation | Direct quantitative PCR absent minor groove binders |
| US10858694B2 (en) * | 2014-12-23 | 2020-12-08 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
| GB201515355D0 (en) * | 2015-08-28 | 2015-10-14 | Ge Healthcare Ltd | A method and kit for analyte detection |
| US11566290B2 (en) | 2016-03-18 | 2023-01-31 | Hummingbird Diagnostics Gmbh | Purification of RNA fractions using a hydrophilic polymeric material |
| CN110343738A (zh) * | 2019-06-17 | 2019-10-18 | 清远市公安局 | 基于fta卡采集和常温保存肋软骨dna的方法和工具 |
| CA3147297A1 (en) * | 2019-07-16 | 2021-01-21 | Meliolabs Inc. | Methods and devices for single-cell based digital high resolution melt |
| EP4237583A2 (en) * | 2020-10-27 | 2023-09-06 | Wallac OY | Methods of analyzing a biological sample |
| KR102291738B1 (ko) * | 2021-01-15 | 2021-08-20 | (주)진이어스 | 컬럼 방식의 단일튜브 pcr용 핵산 분리를 위한 버퍼 조성물 및 이의 용도 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5496562A (en) * | 1988-10-05 | 1996-03-05 | Flinders Technologies Pty Ltd | Solid medium and method for DNA storage |
| US5705345A (en) * | 1991-01-10 | 1998-01-06 | Amersham International Plc | Methods and kits for preparing nucleic acids using cyclodextrin |
| US7122304B2 (en) * | 1997-05-12 | 2006-10-17 | Whatman, Inc. | Methods for the storage and synthesis of nucleic acids using a solid support |
| AU2574499A (en) | 1998-02-02 | 1999-08-16 | Gentra Systems, Inc. | Compositions and methods for using a lysing matrix for isolating dna |
| US6746841B1 (en) | 1999-04-14 | 2004-06-08 | Whatman Inc. | FTA- coated media for use as a molecular diagnostic tool |
| WO2005007852A2 (en) * | 2003-07-09 | 2005-01-27 | Genvault Corporation | Room temperature elution of nucleic acids |
| EP2294225B8 (en) * | 2008-06-30 | 2015-02-18 | Life Technologies Corporation | Method for direct amplification from crude nucleic acid samples |
| WO2010066908A1 (en) * | 2008-12-12 | 2010-06-17 | Eurogentec S.A. | Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions |
| EP2256195A1 (de) * | 2009-05-12 | 2010-12-01 | Qiagen GmbH | Nukleinsäureaufreinigungsverfahren |
| EP2652147B1 (en) * | 2010-12-17 | 2017-01-25 | Wisconsin Alumni Research Foundation | One-step method of elution of dna from blood samples |
| GB201104607D0 (en) * | 2011-03-18 | 2011-05-04 | Ge Healthcare Ltd | Arrangement for preservation of biological samples |
| WO2014041093A1 (en) * | 2012-09-13 | 2014-03-20 | Ge Healthcare Uk Limited | Solid matrix for one step nucleic acid amplification |
| WO2014072354A1 (en) * | 2012-11-09 | 2014-05-15 | Ge Healthcare Uk Limited | Methods for one step nucleic acid amplification of non-eluted samples |
-
2014
- 2014-03-14 EP EP14724852.0A patent/EP2971109B1/en active Active
- 2014-03-14 US US14/774,259 patent/US10053686B2/en active Active
- 2014-03-14 JP JP2016502626A patent/JP6559647B2/ja active Active
- 2014-03-14 WO PCT/US2014/027790 patent/WO2014143714A2/en not_active Ceased
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