JP2017527301A5 - - Google Patents
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- JP2017527301A5 JP2017527301A5 JP2017515830A JP2017515830A JP2017527301A5 JP 2017527301 A5 JP2017527301 A5 JP 2017527301A5 JP 2017515830 A JP2017515830 A JP 2017515830A JP 2017515830 A JP2017515830 A JP 2017515830A JP 2017527301 A5 JP2017527301 A5 JP 2017527301A5
- Authority
- JP
- Japan
- Prior art keywords
- chamber
- microbial
- sample
- genetic material
- diana
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 claims description 42
- 238000000034 method Methods 0.000 claims description 37
- 102000004169 proteins and genes Human genes 0.000 claims description 28
- 108090000623 proteins and genes Proteins 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 17
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 15
- 108020004414 DNA Proteins 0.000 claims description 14
- 210000004027 cell Anatomy 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 12
- 239000003599 detergent Substances 0.000 claims description 11
- 230000006037 cell lysis Effects 0.000 claims description 10
- 230000002934 lysing effect Effects 0.000 claims description 8
- 239000011859 microparticle Substances 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 7
- 238000005349 anion exchange Methods 0.000 claims description 6
- 239000003957 anion exchange resin Substances 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- 241000675916 Serranus phoebe Species 0.000 claims description 4
- 230000009089 cytolysis Effects 0.000 claims description 4
- 230000000779 depleting effect Effects 0.000 claims description 4
- 238000009830 intercalation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- DXHWIAMGTKXUEA-UHFFFAOYSA-O propidium monoazide Chemical compound C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 DXHWIAMGTKXUEA-UHFFFAOYSA-O 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 108091093088 Amplicon Proteins 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- IVRMZWNICZWHMI-UHFFFAOYSA-N Azide Chemical compound [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 claims description 2
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000012139 lysis buffer Substances 0.000 claims description 2
- 239000011534 wash buffer Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 241000124008 Mammalia Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 description 1
- PITRRWWILGYENJ-UHFFFAOYSA-N 2-[2-[2-[2-[2-(4-nonylphenoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCO)C=C1 PITRRWWILGYENJ-UHFFFAOYSA-N 0.000 description 1
- WIHIUFRJMOAJFO-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(4-nonylphenoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 WIHIUFRJMOAJFO-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 229920004929 Triton X-114 Polymers 0.000 description 1
- -1 Tween-40 Polymers 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- FBWNMEQMRUMQSO-UHFFFAOYSA-N tergitol NP-9 Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 FBWNMEQMRUMQSO-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021203271A JP7594523B2 (ja) | 2014-09-17 | 2021-12-15 | 微生物を検出し同定するための方法およびデバイス |
| JP2024107218A JP2024156666A (ja) | 2014-09-17 | 2024-07-03 | 微生物を検出し同定するための方法およびデバイス |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462051447P | 2014-09-17 | 2014-09-17 | |
| US62/051,447 | 2014-09-17 | ||
| PCT/US2015/050741 WO2016044621A1 (en) | 2014-09-17 | 2015-09-17 | Methods and devices for detecting and identifying microorganisms |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021203271A Division JP7594523B2 (ja) | 2014-09-17 | 2021-12-15 | 微生物を検出し同定するための方法およびデバイス |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017527301A JP2017527301A (ja) | 2017-09-21 |
| JP2017527301A5 true JP2017527301A5 (enExample) | 2018-10-25 |
| JP7046603B2 JP7046603B2 (ja) | 2022-04-04 |
Family
ID=55533861
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017515830A Active JP7046603B2 (ja) | 2014-09-17 | 2015-09-17 | 微生物を検出し同定するための方法およびデバイス |
| JP2021203271A Active JP7594523B2 (ja) | 2014-09-17 | 2021-12-15 | 微生物を検出し同定するための方法およびデバイス |
| JP2024107218A Pending JP2024156666A (ja) | 2014-09-17 | 2024-07-03 | 微生物を検出し同定するための方法およびデバイス |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021203271A Active JP7594523B2 (ja) | 2014-09-17 | 2021-12-15 | 微生物を検出し同定するための方法およびデバイス |
| JP2024107218A Pending JP2024156666A (ja) | 2014-09-17 | 2024-07-03 | 微生物を検出し同定するための方法およびデバイス |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US11414712B2 (enExample) |
| EP (2) | EP3696267A1 (enExample) |
| JP (3) | JP7046603B2 (enExample) |
| CA (1) | CA2961510A1 (enExample) |
| WO (1) | WO2016044621A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3696267A1 (en) * | 2014-09-17 | 2020-08-19 | Helixbind Inc. | Methods for lysing eukaryotic cells |
| EP3429751A4 (en) | 2016-03-14 | 2019-12-25 | Helixbind, Inc. | INTEGRATED FLUIDIC DEVICES AND RELATED METHODS |
| CA3058913A1 (en) * | 2017-04-03 | 2018-10-11 | Helixbind, Inc. | Methods and devices for identifying microbial infections |
| WO2019116034A1 (en) * | 2017-12-12 | 2019-06-20 | Cell Therapy Catapult Limited | Microbial enrichment method |
| EP3803326A4 (en) | 2018-05-25 | 2022-02-23 | Qvella Corporation | METHODS AND COMPOSITIONS FOR SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS |
| WO2020214588A1 (en) * | 2019-04-16 | 2020-10-22 | Helixbind, Inc. | Methods and devices for ultrasensitive direct detection of microorganisms |
| WO2021055495A1 (en) * | 2019-09-17 | 2021-03-25 | Juno Bio Limited | Methods and systems for selective nucleic acid depletion |
| US11590507B2 (en) | 2020-02-25 | 2023-02-28 | Helixbind, Inc. | Reagent carriers for fluidic systems |
| CN115703993B (zh) * | 2021-08-17 | 2025-08-01 | 北京京东方技术开发有限公司 | 微流控芯片、其核酸提取方法及核酸提取装置 |
| WO2023141916A1 (zh) | 2022-01-28 | 2023-08-03 | 京东方科技集团股份有限公司 | 芯片散热装置和核酸提取设备 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6534262B1 (en) * | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6664045B1 (en) | 1998-06-18 | 2003-12-16 | Boston Probes, Inc. | PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms |
| US20070054278A1 (en) | 2003-11-18 | 2007-03-08 | Applera Corporation | Polymorphisms in nucleic acid molecules encoding human enzyme proteins, methods of detection and uses thereof |
| DE102005009479A1 (de) * | 2005-03-02 | 2006-09-07 | Molzym Gmbh & Co. Kg | Verwendung von Nukleasen zum Abbau von Nukleinsäure in Gegenwart von chaotropen Agenzien und/oder Tensiden |
| GB0508981D0 (en) | 2005-05-03 | 2005-06-08 | Acolyte Biomedica Ltd | Distinguishing cells in a sample |
| US9434997B2 (en) * | 2007-08-24 | 2016-09-06 | Lawrence Livermore National Security, Llc | Methods, compounds and systems for detecting a microorganism in a sample |
| KR20090058221A (ko) * | 2007-12-04 | 2009-06-09 | 삼성전자주식회사 | 시료 중의 세포 집단으로부터 죽은 세포를 선택적으로파쇄하는 방법 |
| US8609024B2 (en) | 2009-05-15 | 2013-12-17 | Biomerieux, Inc. | System and method for automatically venting and sampling a culture specimen container |
| US20120276530A1 (en) * | 2009-08-24 | 2012-11-01 | Trustees Of Boston University | Label-free sensing of pna-dna complexes using nanopores |
| US9238809B2 (en) * | 2009-09-24 | 2016-01-19 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material |
| EP2325312A1 (de) | 2009-11-19 | 2011-05-25 | Qiagen GmbH | Verfahren zur selektiven Anreicherung und Isolierung mikrobieller und optional zusätzlich viraler Nukleinsäuren |
| EP2333105A1 (en) | 2009-12-08 | 2011-06-15 | Koninklijke Philips Electronics N.V. | Selective lysis of cells |
| WO2011087789A2 (en) * | 2009-12-22 | 2011-07-21 | Becton, Dickinson And Company | Methods for the detection of microorganisms |
| US9228240B2 (en) * | 2010-06-03 | 2016-01-05 | California Institute Of Technology | Methods for detecting and quantifying viable bacterial endo-spores |
| WO2013176992A2 (en) | 2012-05-20 | 2013-11-28 | Alon Singer | Methods and compositions for the diagnosis of sepsis using gamma peptide nucleic acids |
| EP2684947A1 (en) * | 2012-07-09 | 2014-01-15 | Biocartis SA | A method for filtering a biological sample |
| DK2879691T3 (da) * | 2012-08-06 | 2019-06-11 | Biogen Ma Inc | Fremgangsmåder og sammensætninger til inaktivering af kappebærende vira |
| EP3696267A1 (en) * | 2014-09-17 | 2020-08-19 | Helixbind Inc. | Methods for lysing eukaryotic cells |
-
2015
- 2015-09-17 EP EP20154925.0A patent/EP3696267A1/en active Pending
- 2015-09-17 US US15/511,957 patent/US11414712B2/en active Active
- 2015-09-17 EP EP15842027.3A patent/EP3194626B1/en active Active
- 2015-09-17 CA CA2961510A patent/CA2961510A1/en active Pending
- 2015-09-17 WO PCT/US2015/050741 patent/WO2016044621A1/en not_active Ceased
- 2015-09-17 JP JP2017515830A patent/JP7046603B2/ja active Active
-
2021
- 2021-12-15 JP JP2021203271A patent/JP7594523B2/ja active Active
-
2022
- 2022-07-21 US US17/870,506 patent/US20230183820A1/en active Pending
-
2024
- 2024-07-03 JP JP2024107218A patent/JP2024156666A/ja active Pending
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