JP2015530113A - マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ - Google Patents

マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ Download PDF

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JP2015530113A
JP2015530113A JP2015534628A JP2015534628A JP2015530113A JP 2015530113 A JP2015530113 A JP 2015530113A JP 2015534628 A JP2015534628 A JP 2015534628A JP 2015534628 A JP2015534628 A JP 2015534628A JP 2015530113 A JP2015530113 A JP 2015530113A
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primer
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rna
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JP2015530113A5 (enExample
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ラッセル・ヒグチ
エドウィン・ウェイ−ルン・ライ
セルゲイ・ロコフ
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セファイド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2015534628A 2012-09-28 2013-09-25 マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ Pending JP2015530113A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261707670P 2012-09-28 2012-09-28
US61/707,670 2012-09-28
PCT/US2013/061743 WO2014052487A1 (en) 2012-09-28 2013-09-25 Two-primer pcr for microrna multiplex assay

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JP2015530113A true JP2015530113A (ja) 2015-10-15
JP2015530113A5 JP2015530113A5 (enExample) 2016-11-17

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US (1) US20150322510A1 (enExample)
EP (1) EP2900836B1 (enExample)
JP (1) JP2015530113A (enExample)
CA (1) CA2886605A1 (enExample)
WO (1) WO2014052487A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200127495A (ko) * 2019-05-02 2020-11-11 한국과학기술연구원 Rna를 표적으로 하는 증폭 구조체 및 이를 이용한 핵산 증폭 방법
JP2021503903A (ja) * 2017-11-22 2021-02-15 セイフガード バイオシステムズ ホールディングズ リミテッドＳａｆｅｇｕａｒｄ Ｂｉｏｓｙｓｔｅｍｓ Ｈｏｌｄｉｎｇｓ Ｌｔｄ． 非対称ｐｃｒ法
JP2022546443A (ja) * 2019-08-27 2022-11-04 エフ．ホフマン－ラ ロシュ アーゲー ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
DK3194612T3 (da) * 2014-08-22 2024-10-14 Resolution Bioscience Inc Fremgangsmåder til kvantitativ genetisk analyse af cellefrit dna
US10030263B1 (en) * 2014-08-25 2018-07-24 University Of South Florida Multiplexed RNA qPCR assay
WO2016138080A1 (en) * 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins
GB2550712B (en) * 2015-02-26 2021-01-13 Hitachi High Tech Corp Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JP2001513623A (ja) * 1996-07-16 2001-09-04 インタージェン カンパニー 分子エネルギー輸送標識を有する核酸増幅オリゴヌクレオチドおよびそれに基づく方法
JP2010516284A (ja) * 2007-01-26 2010-05-20 ストラタジーン カリフォルニア マイクロｒｎａの検出のための方法、組成物及びキット
WO2011103502A2 (en) * 2010-02-18 2011-08-25 University Of South Florida Materials and methods for profiling micrornas
JP2012516678A (ja) * 2009-02-02 2012-07-26 エクシコン エ／エス 低分子ｒｎａ種の定量化のための方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6770748B2 (en) 1997-03-07 2004-08-03 Takeshi Imanishi Bicyclonucleoside and oligonucleotide analogue
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
EP1179585B1 (en) 1997-12-24 2008-07-09 Cepheid Device and method for lysis
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1208189B1 (en) 1999-05-28 2004-10-06 Cepheid Device and method for analysing liquid samples
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US6262490B1 (en) 1999-11-05 2001-07-17 Advanced Semiconductor Engineering, Inc. Substrate strip for use in packaging semiconductor chips
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US20040259116A1 (en) 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
US20050147975A1 (en) * 2003-12-24 2005-07-07 Schembri Carol T. Methods and compositions for amplification of genomic DNA
DK2038432T3 (en) * 2006-06-30 2017-04-03 Rosetta Genomics Ltd METHOD OF DETECTING AND QUANTIFYING A TARGET NUCLEIC ACID GENERATED BY RT-PCR BY MIRNA
KR101608258B1 (ko) 2009-02-27 2016-04-04 (주)아모레퍼시픽 송진 추출물, 적송엽 추출물 및 적송근 추출물을 함유하는 피부 보습용 화장료 조성물
US8571753B2 (en) 2010-04-14 2013-10-29 Caterpillar Forest Products Inc. Power management system for a forestry machine to automatically adjust engine load

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JP2001513623A (ja) * 1996-07-16 2001-09-04 インタージェン カンパニー 分子エネルギー輸送標識を有する核酸増幅オリゴヌクレオチドおよびそれに基づく方法
JP2010516284A (ja) * 2007-01-26 2010-05-20 ストラタジーン カリフォルニア マイクロｒｎａの検出のための方法、組成物及びキット
JP2012516678A (ja) * 2009-02-02 2012-07-26 エクシコン エ／エス 低分子ｒｎａ種の定量化のための方法
WO2011103502A2 (en) * 2010-02-18 2011-08-25 University Of South Florida Materials and methods for profiling micrornas

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021503903A (ja) * 2017-11-22 2021-02-15 セイフガード バイオシステムズ ホールディングズ リミテッドＳａｆｅｇｕａｒｄ Ｂｉｏｓｙｓｔｅｍｓ Ｈｏｌｄｉｎｇｓ Ｌｔｄ． 非対称ｐｃｒ法
JP7334154B2 (ja) 2017-11-22 2023-08-28 セイフガード バイオシステムズ ホールディングズ リミテッド 非対称ｐｃｒ法
KR20200127495A (ko) * 2019-05-02 2020-11-11 한국과학기술연구원 Rna를 표적으로 하는 증폭 구조체 및 이를 이용한 핵산 증폭 방법
KR102209903B1 (ko) 2019-05-02 2021-02-02 한국과학기술연구원 Rna를 표적으로 하는 증폭 구조체 및 이를 이용한 핵산 증폭 방법
JP2022546443A (ja) * 2019-08-27 2022-11-04 エフ．ホフマン－ラ ロシュ アーゲー ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法
JP7677950B2 (ja) 2019-08-27 2025-05-15 エフ． ホフマン－ラ ロシュ アーゲー ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法

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EP2900836A1 (en) 2015-08-05
EP2900836B1 (en) 2023-05-10
EP2900836A4 (en) 2016-05-18
US20150322510A1 (en) 2015-11-12
WO2014052487A1 (en) 2014-04-03
WO2014052487A8 (en) 2015-04-30
CA2886605A1 (en) 2014-04-03

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