CA2886605A1 - Two-primer pcr for microrna multiplex assay - Google Patents

Two-primer pcr for microrna multiplex assay Download PDF

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Publication number
CA2886605A1
CA2886605A1 CA 2886605 CA2886605A CA2886605A1 CA 2886605 A1 CA2886605 A1 CA 2886605A1 CA 2886605 CA2886605 CA 2886605 CA 2886605 A CA2886605 A CA 2886605A CA 2886605 A1 CA2886605 A1 CA 2886605A1
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CA
Canada
Prior art keywords
sequence
primer
kit
poly
mir
Prior art date
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Pending
Application number
CA 2886605
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English (en)
French (fr)
Inventor
Russell Higuchi
Edwin Wei-Lung LAI
Sergey Lokhov
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Cepheid
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Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Cepheid filed Critical Cepheid
Publication of CA2886605A1 publication Critical patent/CA2886605A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141MicroRNAs, miRNAs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00Applications; Uses
    • C12N2320/10Applications; Uses in screening processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2886605 2012-09-28 2013-09-25 Two-primer pcr for microrna multiplex assay Pending CA2886605A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261707670P 2012-09-28 2012-09-28
US61/707,670 2012-09-28
PCT/US2013/061743 WO2014052487A1 (en) 2012-09-28 2013-09-25 Two-primer pcr for microrna multiplex assay

Publications (1)

Publication Number Publication Date
CA2886605A1 true CA2886605A1 (en) 2014-04-03

Family

ID=50388941

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2886605 Pending CA2886605A1 (en) 2012-09-28 2013-09-25 Two-primer pcr for microrna multiplex assay

Country Status (5)

Country Link
US (1) US20150322510A1 (enExample)
EP (1) EP2900836B1 (enExample)
JP (1) JP2015530113A (enExample)
CA (1) CA2886605A1 (enExample)
WO (1) WO2014052487A1 (enExample)

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EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
DK3194612T3 (da) * 2014-08-22 2024-10-14 Resolution Bioscience Inc Fremgangsmåder til kvantitativ genetisk analyse af cellefrit dna
US10030263B1 (en) * 2014-08-25 2018-07-24 University Of South Florida Multiplexed RNA qPCR assay
WO2016138080A1 (en) * 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins
GB2550712B (en) * 2015-02-26 2021-01-13 Hitachi High Tech Corp Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US10513730B2 (en) * 2017-11-22 2019-12-24 Safeguard Biosystems Holdings Ltd. Asymmetric PCR methods, primers and kits
KR102209903B1 (ko) * 2019-05-02 2021-02-02 한국과학기술연구원 Rna를 표적으로 하는 증폭 구조체 및 이를 이용한 핵산 증폭 방법
CN114286866A (zh) * 2019-08-27 2022-04-05 豪夫迈·罗氏有限公司 用于扩增和检测包括从cccDNA转录的HBV RNA在内的乙型肝炎病毒RNA的组合物和方法
ES3030914T3 (en) * 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer

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EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6770748B2 (en) 1997-03-07 2004-08-03 Takeshi Imanishi Bicyclonucleoside and oligonucleotide analogue
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Also Published As

Publication number Publication date
EP2900836A1 (en) 2015-08-05
EP2900836B1 (en) 2023-05-10
EP2900836A4 (en) 2016-05-18
US20150322510A1 (en) 2015-11-12
JP2015530113A (ja) 2015-10-15
WO2014052487A1 (en) 2014-04-03
WO2014052487A8 (en) 2015-04-30

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