JP2015521030A5 - - Google Patents
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- JP2015521030A5 JP2015521030A5 JP2015505831A JP2015505831A JP2015521030A5 JP 2015521030 A5 JP2015521030 A5 JP 2015521030A5 JP 2015505831 A JP2015505831 A JP 2015505831A JP 2015505831 A JP2015505831 A JP 2015505831A JP 2015521030 A5 JP2015521030 A5 JP 2015521030A5
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- 239000011148 porous material Substances 0.000 claims description 116
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- 108020004414 DNA Proteins 0.000 claims description 63
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- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 46
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- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 28
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 28
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- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 22
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 22
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- 229920000388 Polyphosphate Polymers 0.000 claims description 15
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- 235000011176 polyphosphates Nutrition 0.000 claims description 15
- 229930024421 Adenine Natural products 0.000 claims description 13
- 229960000643 adenine Drugs 0.000 claims description 13
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 13
- 229940104302 cytosine Drugs 0.000 claims description 13
- 125000003342 alkenyl group Chemical group 0.000 claims description 12
- 125000000217 alkyl group Chemical group 0.000 claims description 12
- 125000000304 alkynyl group Chemical group 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 claims description 12
- 150000001720 carbohydrates Chemical class 0.000 claims description 12
- 125000004093 cyano group Chemical group *C#N 0.000 claims description 12
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 12
- 229930195729 fatty acid Natural products 0.000 claims description 12
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- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 12
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 11
- 229920000137 polyphosphoric acid Polymers 0.000 claims description 11
- 229940113082 thymine Drugs 0.000 claims description 11
- 229940035893 uracil Drugs 0.000 claims description 11
- 238000007476 Maximum Likelihood Methods 0.000 claims description 9
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- 239000002243 precursor Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 238000006555 catalytic reaction Methods 0.000 claims description 4
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- 238000001514 detection method Methods 0.000 claims description 4
- 150000007523 nucleic acids Chemical group 0.000 claims description 4
- 238000012935 Averaging Methods 0.000 claims description 3
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- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 101710092462 Alpha-hemolysin Proteins 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- 229940126062 Compound A Drugs 0.000 claims description 2
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 239000003792 electrolyte Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 239000003228 hemolysin Substances 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 235000021317 phosphate Nutrition 0.000 claims description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 2
- 230000000379 polymerizing effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- -1 compound Compound Chemical class 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 27
- 150000002500 ions Chemical class 0.000 description 5
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 159000000032 aromatic acids Chemical class 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
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Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261621981P | 2012-04-09 | 2012-04-09 | |
| US61/621,981 | 2012-04-09 | ||
| US201261662334P | 2012-06-20 | 2012-06-20 | |
| US201261662329P | 2012-06-20 | 2012-06-20 | |
| US201261662330P | 2012-06-20 | 2012-06-20 | |
| US61/662,329 | 2012-06-20 | ||
| US61/662,330 | 2012-06-20 | ||
| US61/662,334 | 2012-06-20 | ||
| US201361781353P | 2013-03-14 | 2013-03-14 | |
| US61/781,353 | 2013-03-14 | ||
| PCT/US2013/035635 WO2013154999A2 (en) | 2012-04-09 | 2013-04-08 | Method of preparation of nanopore and uses thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015521030A JP2015521030A (ja) | 2015-07-27 |
| JP2015521030A5 true JP2015521030A5 (enExample) | 2016-06-09 |
| JP6456816B2 JP6456816B2 (ja) | 2019-01-23 |
Family
ID=49328273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015505831A Expired - Fee Related JP6456816B2 (ja) | 2012-04-09 | 2013-04-08 | ナノ細孔の調製方法およびその使用 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10246479B2 (enExample) |
| EP (1) | EP2836604B1 (enExample) |
| JP (1) | JP6456816B2 (enExample) |
| CN (2) | CN104379761B (enExample) |
| CA (1) | CA2869753A1 (enExample) |
| ES (1) | ES2906186T3 (enExample) |
| WO (1) | WO2013154999A2 (enExample) |
Families Citing this family (114)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| WO2007002204A2 (en) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compostions |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| WO2007053719A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
| DE112007002932B4 (de) | 2006-12-01 | 2015-08-06 | The Trustees Of Columbia University In The City Of New York | Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren |
| EP4310194A3 (en) | 2007-10-19 | 2024-10-16 | The Trustees of Columbia University in the City of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| US9194838B2 (en) | 2010-03-03 | 2015-11-24 | Osaka University | Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide |
| EP2652153B1 (en) | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
| WO2012088339A2 (en) | 2010-12-22 | 2012-06-28 | Genia Technologies, Inc. | Nanopore-based single dna molecule characterization using speed bumps |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| GB2510719A (en) | 2012-06-15 | 2014-08-13 | Genia Technologies Inc | Chip set-up and high-accuracy nucleic acid sequencing |
| EP2864502B1 (en) * | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| CN105102627B (zh) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | 用于检测样品中多种预定化合物的方法 |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| CA2929929A1 (en) | 2013-09-18 | 2015-03-26 | Quantum Biosystems Inc. | Biomolecule sequencing devices, systems and methods |
| JP2015077652A (ja) | 2013-10-16 | 2015-04-23 | クオンタムバイオシステムズ株式会社 | ナノギャップ電極およびその製造方法 |
| EP3640349A3 (en) | 2013-10-23 | 2020-07-29 | Roche Sequencing Solutions, Inc. | High speed molecular sensing with nanopores |
| US9322062B2 (en) | 2013-10-23 | 2016-04-26 | Genia Technologies, Inc. | Process for biosensor well formation |
| CA2943952A1 (en) * | 2014-03-24 | 2015-10-01 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
| US10438811B1 (en) | 2014-04-15 | 2019-10-08 | Quantum Biosystems Inc. | Methods for forming nano-gap electrodes for use in nanosensors |
| WO2015170782A1 (en) | 2014-05-08 | 2015-11-12 | Osaka University | Devices, systems and methods for linearization of polymers |
| US20160003798A1 (en) * | 2014-06-19 | 2016-01-07 | Univ Leland Stanford Junior | Apparatuses and methods for determining analyte charge |
| ES2697428T3 (es) | 2014-07-15 | 2019-01-23 | Illumina Inc | Dispositivo electrónico activado bioquímicamente |
| WO2016065248A1 (en) | 2014-10-23 | 2016-04-28 | Complete Genomics, Inc. | Signal confinement sequencing (scs) and nucleotide analogues for signal confinement sequencing |
| US10060903B2 (en) * | 2014-11-05 | 2018-08-28 | Genia Technologies, Inc. | Exporting measurements of nanopore arrays |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10429342B2 (en) | 2014-12-18 | 2019-10-01 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| EP3256566A4 (en) | 2015-02-10 | 2018-10-17 | Multerra Bio, Inc. | Apparatuses and methods for detecting molecules and binding energy |
| WO2016144973A1 (en) | 2015-03-09 | 2016-09-15 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
| WO2016154215A1 (en) * | 2015-03-23 | 2016-09-29 | The Trustees Of Columbia University In The City Of New York | Polymer tagged nucleotides for single molecule electronic snp assay |
| GB201508669D0 (en) * | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| IL293410B2 (en) * | 2015-06-03 | 2024-01-01 | Illumina Inc | Compositions, Systems, and Methods for Sequencing Polynucleotides using Tethers Anchored to Polymerases Adjacent to Nanopores |
| ES2882583T3 (es) * | 2015-06-23 | 2021-12-02 | Bgi Shenzhen | Electrodo microporoso y método para el análisis de una sustancia química |
| CN108350017B (zh) | 2015-09-10 | 2022-03-15 | 豪夫迈·罗氏有限公司 | 多肽标记的核苷酸及其在通过纳米孔检测的核酸测序中的用途 |
| US11085076B2 (en) | 2015-09-28 | 2021-08-10 | The Trustees Of Columbia University In The City Of New York | Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis |
| CN108521782A (zh) * | 2015-10-21 | 2018-09-11 | 豪夫迈·罗氏有限公司 | 含氟聚合物作为疏水层以支持用于纳米孔的脂质双层形成的用途 |
| CN105368938B (zh) * | 2015-11-06 | 2018-09-21 | 中国科学院重庆绿色智能技术研究院 | 一种基于电击穿在氮化硅薄膜上精确制备纳米孔的方法 |
| WO2017084580A1 (en) * | 2015-11-19 | 2017-05-26 | Peking University | Methods for obtaining and correcting biological sequence information |
| EP3387108B1 (en) * | 2015-12-08 | 2022-05-25 | Quantapore, Inc. | Method of translocating nucleic acids through nanopores |
| JP7280590B2 (ja) * | 2016-01-28 | 2023-05-24 | ロズウェル バイオテクノロジーズ，インコーポレイテッド | 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置 |
| CN106191230A (zh) * | 2016-02-02 | 2016-12-07 | 杭州格磊思沃科技有限公司 | 用氨基酸结合脱氧核苷酸进行dna测序的方法 |
| ES2877193T3 (es) | 2016-04-27 | 2021-11-16 | Quantum Biosystems Inc | Sistemas y métodos para la medición y secuenciación de biomoléculas |
| EP3459115A4 (en) | 2016-05-16 | 2020-04-08 | Agilome, Inc. | GRAPHENE FETING DEVICES, SYSTEMS, AND METHODS OF USE THEREOF FOR NUCLEIC ACID SEQUENCING |
| EP3463381A4 (en) | 2016-05-23 | 2020-01-01 | The Trustees of Columbia University in the City of New York | NUCLEOTIDE DERIVATIVES AND METHOD FOR USE THEREOF |
| US10655174B2 (en) | 2016-05-27 | 2020-05-19 | Roche Sequencing Solutions, Inc. | Tagged multi-nucleotides useful for nucleic acid sequencing |
| WO2017223515A1 (en) | 2016-06-23 | 2017-12-28 | F. Hoffman-La Roche Ag | Formation and calibration of nanopore sequencing cells |
| US11124827B2 (en) | 2016-06-23 | 2021-09-21 | Roche Sequencing Solutions, Inc. | Period-to-period analysis of AC signals from nanopore sequencing |
| EP3497233B1 (en) | 2016-08-08 | 2021-11-10 | F. Hoffmann-La Roche AG | Basecalling for stochastic sequencing processes |
| US10444179B2 (en) | 2016-08-10 | 2019-10-15 | Multerra Bio, Inc. | Apparatuses and methods for detecting molecules and binding energy |
| US10669580B2 (en) | 2016-08-26 | 2020-06-02 | Roche Sequencing Solutions, Inc. | Tagged nucleotides useful for nanopore detection |
| US11345961B2 (en) * | 2016-10-05 | 2022-05-31 | Roche Sequencing Solutions, Inc. | Nucleic acid sequencing using nanotransistors |
| CN109791138B (zh) * | 2016-10-12 | 2021-01-08 | 豪夫迈·罗氏有限公司 | 纳米孔电压方法 |
| JP6653767B2 (ja) * | 2016-12-09 | 2020-02-26 | 株式会社日立ハイテクノロジーズ | ナノポア形成方法、ナノポア形成装置及び生体分子計測装置 |
| US10793901B2 (en) * | 2016-12-28 | 2020-10-06 | Roche Molecular Systems, Inc. | Reversibly protected nucleotide reagents with high thermal stability |
| US11591647B2 (en) | 2017-03-06 | 2023-02-28 | Singular Genomics Systems, Inc. | Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps |
| CN110709089B (zh) | 2017-03-28 | 2024-12-06 | 哥伦比亚大学董事会 | 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物 |
| JP7272967B2 (ja) | 2017-06-20 | 2023-05-12 | イラミーナ インコーポレーテッド | ナノポアシーケンサー |
| WO2019040546A1 (en) * | 2017-08-23 | 2019-02-28 | Roche Sequencing Solutions, Inc | ENZYME SCREENING METHODS |
| WO2019065904A1 (en) * | 2017-09-27 | 2019-04-04 | Quantum Biosystems Inc. | NANOELECTRODES DEVICES AND METHODS OF MANUFACTURING THE SAME |
| WO2019073774A1 (ja) * | 2017-10-11 | 2019-04-18 | ソニーセミコンダクタソリューションズ株式会社 | センサ装置及び測定機器 |
| US20210318302A1 (en) * | 2017-11-27 | 2021-10-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Amino acid-modified nanopores and uses thereof |
| EP3732484B1 (en) | 2017-12-28 | 2023-06-07 | F. Hoffmann-La Roche AG | Measuring and removing noise in stochastic signals from a nanopore dna sequencing system driven by an alternating signal |
| EP3527673A1 (en) * | 2018-02-15 | 2019-08-21 | QIAGEN GmbH | Sequencing method |
| US12215124B2 (en) | 2018-02-21 | 2025-02-04 | Singular Genomics Systems, Inc. | Fluorinated nucleotides and uses thereof |
| WO2019166457A1 (en) | 2018-02-28 | 2019-09-06 | F. Hoffmann-La Roche Ag | Tagged nucleoside compounds useful for nanopore detection |
| WO2019178393A1 (en) | 2018-03-15 | 2019-09-19 | The Trustees Of Columbia University In The City Of New York | Nucleotide analogues and use thereof for nucleic acid sequencing and analysis |
| EP3791179A4 (en) | 2018-05-09 | 2022-01-26 | Arizona Board of Regents on behalf of Arizona State University | METHODS FOR ELECTRONIC DETECTION AND QUANTIFICATION OF ANTIBODIES |
| IL278769B2 (en) | 2018-05-17 | 2025-06-01 | Stuart Lindsay | Device, system and method for direct electrical measurement of enzyme activity |
| CN112534063A (zh) | 2018-05-22 | 2021-03-19 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
| CN108645905B (zh) * | 2018-05-24 | 2019-12-03 | 南通大学 | 一种基于固态纳米孔检测过氧化氢的方法 |
| CN113227772B (zh) * | 2018-07-23 | 2024-11-01 | 纽约市哥伦比亚大学信托人 | 用于生物标志物检测的单分子电子多重纳米孔免疫测定 |
| TWI676193B (zh) | 2018-07-31 | 2019-11-01 | 國家中山科學研究院 | 一種氮磷共摻雜的多孔石墨烯結合離子液體電解質的超高電容器及其製作方法 |
| EP3867408A1 (en) | 2018-10-19 | 2021-08-25 | F. Hoffmann-La Roche AG | Electric field-assisted junctions for sequencing |
| WO2020086834A1 (en) | 2018-10-25 | 2020-04-30 | Singular Genomics Systems, Inc. | Nucleotide analogues |
| CN114599796B (zh) * | 2018-11-29 | 2025-03-07 | 通用测序技术公司 | 生物聚合物测序系统 |
| ES3016009T3 (en) * | 2018-12-11 | 2025-05-08 | Hoffmann La Roche | Systems and methods for self-limiting protein pore insertion in a membrane |
| ES2988083T3 (es) | 2018-12-19 | 2024-11-19 | Hoffmann La Roche | Nucleótidos protegidos reversiblemente en 3' para secuenciación por síntesis usando nanoporos |
| CN113453692A (zh) | 2019-01-08 | 2021-09-28 | 奇异基因组学系统公司 | 核苷酸可裂解接头和其用途 |
| AU2020214334A1 (en) | 2019-01-30 | 2021-08-12 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic circuits, systems and methods for preparing and using them |
| WO2020167447A2 (en) | 2019-02-15 | 2020-08-20 | Illumina, Inc. | Sensing systems |
| CN113710686A (zh) | 2019-02-19 | 2021-11-26 | 阿尔缇玛基因组学公司 | 用于光学检测和测序的接头和方法 |
| EP3977137B1 (en) * | 2019-05-29 | 2026-04-01 | Nanjing University | Detection of analytes by nanopore without using electrodes |
| WO2021156370A1 (en) | 2020-02-06 | 2021-08-12 | F. Hoffmann-La Roche Ag | Compositions that reduce template threading into a nanopore |
| US11807851B1 (en) | 2020-02-18 | 2023-11-07 | Ultima Genomics, Inc. | Modified polynucleotides and uses thereof |
| CN115380120A (zh) | 2020-02-28 | 2022-11-22 | 代表亚利桑那大学的亚利桑那校董事会 | 用于对生物聚合物进行测序的方法 |
| CN115398008B (zh) * | 2020-04-14 | 2025-05-09 | 成都今是科技有限公司 | 纳米孔制备和检测方法及其检测装置 |
| CN115552030A (zh) | 2020-04-17 | 2022-12-30 | 代表亚利桑那大学的亚利桑那校董事会 | 单分子电子序列检测器及其使用方法 |
| AU2021263432A1 (en) * | 2020-04-30 | 2022-12-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for sequencing biopolymers |
| US12276653B2 (en) | 2020-05-29 | 2025-04-15 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic devices with programmable adaptors |
| WO2022033998A1 (en) | 2020-08-11 | 2022-02-17 | F. Hoffmann-La Roche Ag | Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same |
| WO2022140655A1 (en) * | 2020-12-23 | 2022-06-30 | The Trustees Of Columbia University In The City Of New York | Single-molecule electronic multiplex nanopore immunoassays for biomarker detection |
| CN117255945A (zh) | 2021-03-31 | 2023-12-19 | 伊鲁米纳公司 | 纳米孔传感器装置 |
| US12624389B2 (en) | 2021-06-07 | 2026-05-12 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for sequencing biopolymers |
| CN118056019A (zh) | 2021-06-17 | 2024-05-17 | 豪夫迈·罗氏有限公司 | 具有穿过通道的带负电荷的聚合物的工程化纳米孔 |
| EP4355757A1 (en) | 2021-06-17 | 2024-04-24 | F. Hoffmann-La Roche AG | Nucleoside-5 -oligophosphates having a cationically-modified nucleobase |
| CN118318049A (zh) | 2021-09-28 | 2024-07-09 | 安序源有限公司 | 用于处理核酸样品的方法以及核酸样品的组合物 |
| US11946894B2 (en) * | 2022-02-16 | 2024-04-02 | Western Digital Technologies, Inc. | Low noise amplifiers with feedback for nanopore applications |
| US11940404B2 (en) | 2022-02-16 | 2024-03-26 | Western Digital Technologies, Inc. | Low noise amplifiers with shields for nanopore Applications |
| US20250109433A1 (en) * | 2023-09-22 | 2025-04-03 | Illumina, Inc. | Macromolecule blocks for nanopore sequencing |
| US12523644B2 (en) | 2023-10-31 | 2026-01-13 | Western Digital Technologies, Inc. | Methods and systems for nucleic acid sequencing using nanopores |
| US20250137990A1 (en) * | 2023-10-31 | 2025-05-01 | Western Digital Technologies, Inc. | Nucleic acid sequencing using nanopores |
| WO2025128542A1 (en) * | 2023-12-11 | 2025-06-19 | University Of Washington | Nanopore sequencing dna by replacement of nucleotides with analogs |
| CN117947165B (zh) * | 2024-01-19 | 2024-10-08 | 西安交通大学医学院第二附属医院 | PCR检测肺腺癌癌前病变人群血浆lncRNA的检测方法 |
| WO2026017094A1 (zh) * | 2024-07-16 | 2026-01-22 | 成都今是科技有限公司 | 核酸测序系统 |
| CN119673285B (zh) * | 2024-11-14 | 2026-01-27 | 河南华之源智造科技有限公司 | 一种生物纳米孔对dna核酸生物体进行确定的数据处理方法 |
Family Cites Families (127)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| WO1993017127A1 (en) | 1992-02-20 | 1993-09-02 | The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Boomerand dna amplification |
| JPH07508342A (ja) | 1992-04-22 | 1995-09-14 | エコール ポリテクニーク フェデラル ドゥ ローザンヌ（エーペーエフエル） | 脂質膜センサー |
| GB9315847D0 (en) | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
| US20120160687A1 (en) | 1995-03-17 | 2012-06-28 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5804386A (en) | 1997-01-15 | 1998-09-08 | Incyte Pharmaceuticals, Inc. | Sets of labeled energy transfer fluorescent primers and their use in multi component analysis |
| US5876936A (en) | 1997-01-15 | 1999-03-02 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators |
| US6046005A (en) | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
| US6485703B1 (en) | 1998-07-31 | 2002-11-26 | The Texas A&M University System | Compositions and methods for analyte detection |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US20030054360A1 (en) | 1999-01-19 | 2003-03-20 | Larry Gold | Method and apparatus for the automated generation of nucleic acid ligands |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| AU5631200A (en) | 1999-06-22 | 2001-01-09 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| CA2384100C (en) | 1999-09-07 | 2011-03-29 | The Regents Of The University Of California | Methods of determining the presence of double stranded nucleic acids in a sample |
| US6399335B1 (en) | 1999-11-16 | 2002-06-04 | Advanced Research And Technology Institute, Inc. | γ-phosphoester nucleoside triphosphates |
| DE19956753A1 (de) | 1999-11-25 | 2001-06-28 | Schott Glas | Verfahren und Vorrichtung zum Tempern von flachen Körpern |
| US6383749B2 (en) | 1999-12-02 | 2002-05-07 | Clontech Laboratories, Inc. | Methods of labeling nucleic acids for use in array based hybridization assays |
| EP2083015B1 (en) | 2000-02-11 | 2016-04-06 | The Texas A & M University System | Biosensor compositions and methods of use |
| US6616895B2 (en) | 2000-03-23 | 2003-09-09 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| AU2001268291A1 (en) * | 2000-06-07 | 2001-12-17 | Li-Cor, Inc. | Charge-switch nucleotides |
| US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| EP1307469B1 (en) | 2000-08-03 | 2008-01-23 | Boehringer Mannheim Gmbh | Nucleic acid binding compounds containing pyrazolo¬3,4-d pyrimidine analogues of purin-2,6-diamine and their uses |
| US6627748B1 (en) | 2000-09-11 | 2003-09-30 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses |
| US20060057565A1 (en) | 2000-09-11 | 2006-03-16 | Jingyue Ju | Combinatorial fluorescence energy transfer tags and uses thereof |
| CA2421582A1 (en) | 2000-09-11 | 2002-03-21 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and uses thereof |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| US20030027140A1 (en) | 2001-03-30 | 2003-02-06 | Jingyue Ju | High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry |
| US20030104428A1 (en) | 2001-06-21 | 2003-06-05 | President And Fellows Of Harvard College | Method for characterization of nucleic acid molecules |
| US7041812B2 (en) | 2001-08-29 | 2006-05-09 | Amersham Biosciences Corp | Labeled nucleoside polyphosphates |
| WO2003064621A2 (en) | 2002-02-01 | 2003-08-07 | Ambion, Inc. | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES |
| WO2003080861A1 (en) * | 2002-03-22 | 2003-10-02 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Single molecule sequencing using phosphate labeled nucleotides |
| US7005264B2 (en) | 2002-05-20 | 2006-02-28 | Intel Corporation | Method and apparatus for nucleic acid sequencing and identification |
| DE60333365D1 (de) | 2002-05-30 | 2010-08-26 | Sloan Kettering Inst Cancer | Kinasesuppressor der ras-inaktivierung zur therapie von durch ras vermittelter tumorgenese |
| US7074597B2 (en) | 2002-07-12 | 2006-07-11 | The Trustees Of Columbia University In The City Of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
| US7355216B2 (en) | 2002-12-09 | 2008-04-08 | The Regents Of The University Of California | Fluidic nanotubes and devices |
| WO2004055160A2 (en) | 2002-12-13 | 2004-07-01 | The Trustees Of Columbia University In The City Of New York | Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry |
| US6870361B2 (en) | 2002-12-21 | 2005-03-22 | Agilent Technologies, Inc. | System with nano-scale conductor and nano-opening |
| JP4896708B2 (ja) | 2003-02-05 | 2012-03-14 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 新規リンカー類を有する末端リン酸標識ヌクレオチド類 |
| EP1597602A4 (en) | 2003-02-05 | 2009-07-22 | Ge Healthcare Bio Sciences | SOLID PHASE SEQUENCING |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| WO2005017025A2 (en) | 2003-08-15 | 2005-02-24 | The President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7019346B2 (en) | 2003-12-23 | 2006-03-28 | Intel Corporation | Capacitor having an anodic metal oxide substrate |
| CA2557818A1 (en) | 2004-03-03 | 2005-09-15 | The Trustees Of Columbia University In The City Of New York | Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US20050239134A1 (en) | 2004-04-21 | 2005-10-27 | Board Of Regents, The University Of Texas System | Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein |
| GB2431013B (en) | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| CA2577079C (en) | 2004-08-13 | 2014-05-20 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore dna readout platform |
| US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
| WO2007084103A2 (en) | 2004-12-21 | 2007-07-26 | The Texas A & M University System | High temperature ion channels and pores |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| WO2007002204A2 (en) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compostions |
| WO2007041621A2 (en) | 2005-10-03 | 2007-04-12 | Xingsheng Sean Ling | Hybridization assisted nanopore sequencing |
| US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| WO2007053719A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| EP1974057B1 (en) * | 2005-12-21 | 2012-06-13 | Roche Diagnostics GmbH | Sequencing and genotyping using reversibly 2'-modified nucleotides |
| EP1963530B1 (en) | 2005-12-22 | 2011-07-27 | Pacific Biosciences of California, Inc. | Active surface coupled polymerases |
| US20070298511A1 (en) | 2006-04-27 | 2007-12-27 | The Texas A&M University System | Nanopore sensor system |
| US7777505B2 (en) | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
| CN1932039B (zh) * | 2006-09-21 | 2010-06-16 | 上海交通大学 | 外切酶-纳米孔的单分子核酸测序方法 |
| DE112007002932B4 (de) | 2006-12-01 | 2015-08-06 | The Trustees Of Columbia University In The City Of New York | Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| US20100196203A1 (en) | 2007-02-20 | 2010-08-05 | Gurdial Singh Sanghera | Formation of Lipid Bilayers |
| WO2008124107A1 (en) * | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| EP3543357A1 (en) * | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| US20090073293A1 (en) | 2007-06-27 | 2009-03-19 | Yoel Yaffe | CMOS image sensors with increased dynamic range and methods of operating the same |
| GB0713143D0 (en) | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
| WO2009046094A1 (en) | 2007-10-01 | 2009-04-09 | Nabsys, Inc. | Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment |
| EP2205765B1 (en) | 2007-10-02 | 2012-09-12 | President and Fellows of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| EP4310194A3 (en) | 2007-10-19 | 2024-10-16 | The Trustees of Columbia University in the City of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| ATE530497T1 (de) | 2008-03-31 | 2011-11-15 | Sony Deutschland Gmbh | Verfahren zur herstellung einer membran mit konischer pore |
| EP2274446B1 (en) | 2008-03-31 | 2015-09-09 | Pacific Biosciences of California, Inc. | Two slow-step polymerase enzyme systems and methods |
| WO2009134469A1 (en) | 2008-04-29 | 2009-11-05 | Life Technologies | Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use |
| US8940142B2 (en) | 2008-05-05 | 2015-01-27 | The Regents Of The University Of California | Functionalized nanopipette biosensor |
| EP2307540B1 (en) * | 2008-07-07 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| US8921046B2 (en) | 2008-09-19 | 2014-12-30 | Pacific Biosciences Of California, Inc. | Nucleic acid sequence analysis |
| US20100148126A1 (en) | 2008-11-26 | 2010-06-17 | Board Of Regents, The University Of Texas System | Genomic sequencing using modified protein pores and ionic liquids |
| US20100227414A1 (en) | 2009-03-05 | 2010-09-09 | Trex Enterprises Corp. | Affinity capture mass spectroscopy with a porous silicon biosensor |
| GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| US20130053252A1 (en) | 2009-09-25 | 2013-02-28 | President & Fellows Of Harvard College | Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides |
| EP2483680A4 (en) | 2009-09-30 | 2014-01-01 | Quantapore Inc | ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE |
| US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| US20110287414A1 (en) | 2010-02-08 | 2011-11-24 | Genia Technologies, Inc. | Systems and methods for identifying a portion of a molecule |
| CA2995178C (en) | 2010-02-08 | 2023-09-12 | Genia Technologies, Inc. | Method and system for forming a nanopore in a lipid bilayer |
| US20120052188A1 (en) | 2010-02-08 | 2012-03-01 | Genia Technologies, Inc. | Systems and methods for assembling a lipid bilayer on a substantially planar solid surface |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| US20110192723A1 (en) | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for manipulating a molecule in a nanopore |
| WO2011106456A2 (en) | 2010-02-23 | 2011-09-01 | University Of Washington | Artificial mycolic acid membranes |
| US8652779B2 (en) * | 2010-04-09 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using charge blockade labels |
| EP3190193A1 (en) | 2010-07-14 | 2017-07-12 | The Curators Of The University Of Missouri | Nanopore-facilitated single molecule detection of nucleic acids |
| EP2652153B1 (en) * | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
| WO2012088339A2 (en) | 2010-12-22 | 2012-06-28 | Genia Technologies, Inc. | Nanopore-based single dna molecule characterization using speed bumps |
| US8962242B2 (en) | 2011-01-24 | 2015-02-24 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
| US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
| WO2012109574A2 (en) | 2011-02-11 | 2012-08-16 | Nabsys, Inc. | Assay methods using dna binding proteins |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| CN104011866B (zh) | 2011-07-27 | 2017-06-09 | 伊利诺伊大学评议会 | 用于生物分子表征的纳米孔传感器 |
| WO2013109970A1 (en) * | 2012-01-20 | 2013-07-25 | Genia Technologies, Inc. | Nanopore based molecular detection and sequencing |
| US8541849B2 (en) | 2012-02-14 | 2013-09-24 | Genia Technologies, Inc. | Noise shielding techniques for ultra low current measurements in biochemical applications |
| EP2814983B1 (en) | 2012-02-16 | 2019-04-24 | Genia Technologies, Inc. | Methods for creating bilayers for use with nanopore sensors |
| US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| GB2510719A (en) * | 2012-06-15 | 2014-08-13 | Genia Technologies Inc | Chip set-up and high-accuracy nucleic acid sequencing |
| EP2864502B1 (en) | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| CN105102627B (zh) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | 用于检测样品中多种预定化合物的方法 |
| US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| US10718011B2 (en) | 2014-02-12 | 2020-07-21 | The Trustees Of Columbia University In The City Of New York | Single molecule electronic multiplex SNP assay and PCR analysis |
| CA2943952A1 (en) | 2014-03-24 | 2015-10-01 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
| US20170101675A1 (en) | 2014-05-19 | 2017-04-13 | The Trustees Of Columbia University In The City Of New York | Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators |
| WO2016144973A1 (en) | 2015-03-09 | 2016-09-15 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
| WO2016154215A1 (en) | 2015-03-23 | 2016-09-29 | The Trustees Of Columbia University In The City Of New York | Polymer tagged nucleotides for single molecule electronic snp assay |
| US11085076B2 (en) | 2015-09-28 | 2021-08-10 | The Trustees Of Columbia University In The City Of New York | Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis |
| US20180327828A1 (en) | 2015-11-18 | 2018-11-15 | The Trustees Of Columbia University In The City Of New York | Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators |
| WO2017176677A1 (en) | 2016-04-04 | 2017-10-12 | The Trustees Of Columbia University In The City Of New York | Flourescence energy transfer-based single molecule/ensemble dna sequencing by synthesis |
| WO2017176679A1 (en) | 2016-04-04 | 2017-10-12 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis with nucleotide analogues and raman detection |
| EP3463381A4 (en) | 2016-05-23 | 2020-01-01 | The Trustees of Columbia University in the City of New York | NUCLEOTIDE DERIVATIVES AND METHOD FOR USE THEREOF |
| CN110709089B (zh) | 2017-03-28 | 2024-12-06 | 哥伦比亚大学董事会 | 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物 |
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- 2013-04-08 EP EP13775787.8A patent/EP2836604B1/en active Active
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