JP2015510392A - 低減したispa活性を有する宿主細胞を用いるイソプレン産生増強法 - Google Patents

低減したispa活性を有する宿主細胞を用いるイソプレン産生増強法 Download PDF

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JP2015510392A
JP2015510392A JP2014548993A JP2014548993A JP2015510392A JP 2015510392 A JP2015510392 A JP 2015510392A JP 2014548993 A JP2014548993 A JP 2014548993A JP 2014548993 A JP2014548993 A JP 2014548993A JP 2015510392 A JP2015510392 A JP 2015510392A
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polypeptide
cell
ispa
isoprene
nucleic acids
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JP2015510392A5 (https=
Inventor
マコーリフ、ジョセフ・シー
ミューア、レイチェル・イー
ニールセン、アレックス・ティー
ペレス、キャロライン・エム
ヴァヴィリン、ドミトリ・ブイ
ウェルス、デレック・エイチ
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Goodyear Tire and Rubber Co
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Goodyear Tire and Rubber Co
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Publication of JP2015510392A publication Critical patent/JP2015510392A/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001Dimethylallyltranstransferase (2.5.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/03Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027Isoprene synthase (4.2.3.27)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014548993A 2011-12-23 2012-12-21 低減したispa活性を有する宿主細胞を用いるイソプレン産生増強法 Pending JP2015510392A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161580163P 2011-12-23 2011-12-23
US61/580,163 2011-12-23
US201261639855P 2012-04-27 2012-04-27
US61/639,855 2012-04-27
PCT/US2012/071518 WO2013096925A1 (en) 2011-12-23 2012-12-21 Enhanced production of isoprene using host cells having decreased ispa activity

Publications (2)

Publication Number Publication Date
JP2015510392A true JP2015510392A (ja) 2015-04-09
JP2015510392A5 JP2015510392A5 (https=) 2016-02-12

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JP2014548993A Pending JP2015510392A (ja) 2011-12-23 2012-12-21 低減したispa活性を有する宿主細胞を用いるイソプレン産生増強法

Country Status (10)

Country Link
US (2) US8975051B2 (https=)
EP (1) EP2794863A1 (https=)
JP (1) JP2015510392A (https=)
CN (1) CN104245927A (https=)
AU (1) AU2012358149A1 (https=)
BR (1) BR112014014947A2 (https=)
CA (1) CA2859885A1 (https=)
MX (1) MX2014007623A (https=)
SG (1) SG11201403163QA (https=)
WO (1) WO2013096925A1 (https=)

Cited By (2)

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JP2018014960A (ja) * 2016-07-29 2018-02-01 横浜ゴム株式会社 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド
JP2023524097A (ja) * 2020-04-30 2023-06-08 ストロ バイオファーマ, インコーポレイテッド 大腸菌を用いた全長抗体の作製方法

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US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
EP2885420B1 (en) 2012-08-17 2018-12-05 Evolva SA Increased production of terpenes and terpenoids
WO2015083769A1 (ja) * 2013-12-04 2015-06-11 国立研究開発法人理化学研究所 光合成のみによるポリヒドロキシアルカン酸の生産
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11549117B2 (en) 2017-05-11 2023-01-10 Industry-Academic Cooperation Foundation Gyeongsang National University Marker composition for selecting living modified organism, living modified organism, and transformation method
WO2018208116A1 (ko) * 2017-05-11 2018-11-15 경상대학교산학협력단 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법
CN110317765B (zh) * 2018-03-29 2020-11-20 中国科学院天津工业生物技术研究所 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用
WO2020112647A1 (en) * 2018-11-27 2020-06-04 Khona Scientific Llc Bidirectional multi-enzymatic scaffolds for biosynthesizing cannabinoids
CN112553134B (zh) * 2020-12-30 2022-09-06 江南大学 一种在枯草芽孢杆菌中表达α-淀粉酶的方法

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
JP2018014960A (ja) * 2016-07-29 2018-02-01 横浜ゴム株式会社 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド
JP2023524097A (ja) * 2020-04-30 2023-06-08 ストロ バイオファーマ, インコーポレイテッド 大腸菌を用いた全長抗体の作製方法
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Publication number Publication date
US9388431B2 (en) 2016-07-12
EP2794863A1 (en) 2014-10-29
CN104245927A (zh) 2014-12-24
US20150218590A1 (en) 2015-08-06
SG11201403163QA (en) 2014-09-26
US8975051B2 (en) 2015-03-10
BR112014014947A2 (pt) 2020-10-27
US20130164808A1 (en) 2013-06-27
CA2859885A1 (en) 2013-06-27
MX2014007623A (es) 2015-02-04
WO2013096925A1 (en) 2013-06-27
AU2012358149A1 (en) 2014-07-03

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