JP2015509366A - 神経幹細胞および運動ニューロンの生成 - Google Patents

神経幹細胞および運動ニューロンの生成 Download PDF

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JP2015509366A
JP2015509366A JP2014558252A JP2014558252A JP2015509366A JP 2015509366 A JP2015509366 A JP 2015509366A JP 2014558252 A JP2014558252 A JP 2014558252A JP 2014558252 A JP2014558252 A JP 2014558252A JP 2015509366 A JP2015509366 A JP 2015509366A
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mir
mirna
cells
differentiation
group
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JP2015509366A5 (enExample
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チャヤ ブローディー，
チャヤ ブローディー，
シモン スラヴィン，
シモン スラヴィン，
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Henry Ford Health System
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Henry Ford Health System
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JP2014558252A 2012-02-22 2013-02-21 神経幹細胞および運動ニューロンの生成 Pending JP2015509366A (ja)

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US201261601596P 2012-02-22 2012-02-22
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PCT/IB2013/051429 WO2013124816A2 (en) 2012-02-22 2013-02-21 Generation of neural stem cells and motor neurons

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017115789A1 (ja) * 2015-12-28 2017-07-06 国立研究開発法人理化学研究所 加齢による生理機能の低下を回復または改善する組成物
JP2019509065A (ja) * 2016-03-16 2019-04-04 サイナータ セラピューティクス リミテッド コロニー形成培地及びその使用
JP2019529425A (ja) * 2016-09-19 2019-10-17 フォンダツィオーネ・イスティトゥート・イタリアーノ・ディ・テクノロジャＦｏｎｄａｚｉｏｎｅ Ｉｓｔｉｔｕｔｏ Ｉｔａｌｉａｎｏ Ｄｉ Ｔｅｃｎｏｌｏｇｉａ ｍｉＲＮＡ医薬組成物およびその治療的使用
WO2023238948A1 (ja) * 2022-06-09 2023-12-14 国立大学法人九州大学 ｍｉＲＮＡを含む新規脳神経新生促進剤

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2354246A1 (en) 2010-02-05 2011-08-10 febit holding GmbH miRNA in the diagnosis of ovarian cancer
US10385314B2 (en) 2010-08-16 2019-08-20 Exostem Biotec Ltd. Methods of generating oligodendrocytes and cell populations comprising same
US9783781B2 (en) 2010-08-16 2017-10-10 Exostem Biotec Ltd. Methods of generating oligodendrocytes and cell populations comprising same
JP2015509366A (ja) 2012-02-22 2015-03-30 ブレインステム バイオテック リミテッド 神経幹細胞および運動ニューロンの生成
US10034902B2 (en) 2012-02-22 2018-07-31 Exostem Biotec Ltd. MicroRNAs for the generation of astrocytes
US20150079046A1 (en) * 2012-04-03 2015-03-19 Reneuron Limited Stem cell microparticles
GB201317887D0 (en) 2013-10-09 2013-11-20 Reneuron Ltd Product
WO2015051510A1 (zh) * 2013-10-09 2015-04-16 中山大学 一种小分子rna及其制备方法和在特异性上调基因转录活性的药物中的应用
WO2015091892A1 (en) * 2013-12-19 2015-06-25 Comprehensive Biomarker Center Gmbh Mirnas as non-invasive biomarkers for parkinson's disease
WO2016138287A1 (en) * 2015-02-25 2016-09-01 Washington University METHODS TO DETECT MOTOR NEURON DISEASE COMPRISING MICRO-RNAs
EP3273971A4 (en) * 2015-03-27 2018-12-05 Yeda Research and Development Co. Ltd. Methods of treating motor neuron diseases
WO2016160918A1 (en) 2015-03-31 2016-10-06 The University Of North Carolina At Chapel Hill Delivery vehicles for stem cells and uses thereof
JP2019535691A (ja) * 2016-11-03 2019-12-12 エグゾステム バイオテック リミテッド 間葉系幹細胞集団、それらの生成物およびそれらの使用
IT201700012604A1 (it) * 2017-02-06 2018-08-06 Consiglio Naz Delle Ricerche Cnr Nuovi regolatori dell’angiogenesi
KR102113647B1 (ko) 2017-03-30 2020-05-22 울산대학교 산학협력단 신경줄기세포 사멸 억제 효과를 가지는 miR-494-3p 억제제를 유효성분으로 포함하는 퇴행성 신경질환 예방 또는 치료용 조성물
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KR101964748B1 (ko) 2017-06-01 2019-04-03 울산대학교 산학협력단 신경줄기세포 사멸 억제 효과를 가지는 miR-141-5p 억제제를 유효성분으로 포함하는 퇴행성 신경질환 예방 또는 치료용 조성물
CN107982537A (zh) * 2017-11-17 2018-05-04 厦门大学 针对microRNA-155的治疗性药物及其应用
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WO2020037254A1 (en) * 2018-08-17 2020-02-20 City Of Hope Compounds of chemically modified oligonucleotides and methods of use thereof
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US20230372403A1 (en) * 2020-10-09 2023-11-23 The Regents Of The University Of California Immunoengineering biomaterials for treatment of graft rejection
KR102791586B1 (ko) 2021-11-23 2025-04-08 재단법인 아산사회복지재단 miR-92b-5p 억제제를 유효성분으로 포함하는 퇴행성 뇌신경질환 예방 또는 치료용 조성물
CN116333994A (zh) * 2021-12-07 2023-06-27 上海鲸奇生物科技有限公司 非编码rna介导的神经性疾病治疗

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144698A2 (en) * 2009-06-10 2010-12-16 Henry Ford Health System Methods, systems, and compositions for neuronal differentiation of multipotent stromal cells

Family Cites Families (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5023243A (en) 1981-10-23 1991-06-11 Molecular Biosystems, Inc. Oligonucleotide therapeutic agent and method of making same
US4476301A (en) 1982-04-29 1984-10-09 Centre National De La Recherche Scientifique Oligonucleotides, a process for preparing the same and their application as mediators of the action of interferon
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US5550111A (en) 1984-07-11 1996-08-27 Temple University-Of The Commonwealth System Of Higher Education Dual action 2',5'-oligoadenylate antiviral derivatives and uses thereof
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US5166315A (en) 1989-12-20 1992-11-24 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5405938A (en) 1989-12-20 1995-04-11 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5185444A (en) 1985-03-15 1993-02-09 Anti-Gene Deveopment Group Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5264423A (en) 1987-03-25 1993-11-23 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
US5276019A (en) 1987-03-25 1994-01-04 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
US5188897A (en) 1987-10-22 1993-02-23 Temple University Of The Commonwealth System Of Higher Education Encapsulated 2',5'-phosphorothioate oligoadenylates
US4924624A (en) 1987-10-22 1990-05-15 Temple University-Of The Commonwealth System Of Higher Education 2,',5'-phosphorothioate oligoadenylates and plant antiviral uses thereof
WO1989009221A1 (en) 1988-03-25 1989-10-05 University Of Virginia Alumni Patents Foundation Oligonucleotide n-alkylphosphoramidates
US5278302A (en) 1988-05-26 1994-01-11 University Patents, Inc. Polynucleotide phosphorodithioates
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
CA2006008C (en) 1988-12-20 2000-02-15 Donald J. Kessler Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5399676A (en) 1989-10-23 1995-03-21 Gilead Sciences Oligonucleotides with inverted polarity
US5264562A (en) 1989-10-24 1993-11-23 Gilead Sciences, Inc. Oligonucleotide analogs with novel linkages
US5264564A (en) 1989-10-24 1993-11-23 Gilead Sciences Oligonucleotide analogs with novel linkages
US5177198A (en) 1989-11-30 1993-01-05 University Of N.C. At Chapel Hill Process for preparing oligoribonucleoside and oligodeoxyribonucleoside boranophosphates
US5587361A (en) 1991-10-15 1996-12-24 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US5321131A (en) 1990-03-08 1994-06-14 Hybridon, Inc. Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
US5470967A (en) 1990-04-10 1995-11-28 The Dupont Merck Pharmaceutical Company Oligonucleotide analogs with sulfamate linkages
US5489677A (en) 1990-07-27 1996-02-06 Isis Pharmaceuticals, Inc. Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5618704A (en) 1990-07-27 1997-04-08 Isis Pharmacueticals, Inc. Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling
US5610289A (en) 1990-07-27 1997-03-11 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogues
US5623070A (en) 1990-07-27 1997-04-22 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5608046A (en) 1990-07-27 1997-03-04 Isis Pharmaceuticals, Inc. Conjugated 4'-desmethyl nucleoside analog compounds
US5677437A (en) 1990-07-27 1997-10-14 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5541307A (en) 1990-07-27 1996-07-30 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs and solid phase synthesis thereof
KR100211552B1 (ko) 1990-08-03 1999-08-02 디. 꼬쉬 유전자 발현 억제용 화합물 및 방법
US5177196A (en) 1990-08-16 1993-01-05 Microprobe Corporation Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof
US5214134A (en) 1990-09-12 1993-05-25 Sterling Winthrop Inc. Process of linking nucleosides with a siloxane bridge
US5561225A (en) 1990-09-19 1996-10-01 Southern Research Institute Polynucleotide analogs containing sulfonate and sulfonamide internucleoside linkages
JPH06505704A (ja) 1990-09-20 1994-06-30 ギリアド　サイエンシズ，インコーポレイテッド 改変ヌクレオシド間結合
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US6933286B2 (en) 1991-03-19 2005-08-23 R. Martin Emanuele Therapeutic delivery compositions and methods of use thereof
US20020123476A1 (en) 1991-03-19 2002-09-05 Emanuele R. Martin Therapeutic delivery compositions and methods of use thereof
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5571799A (en) 1991-08-12 1996-11-05 Basco, Ltd. (2'-5') oligoadenylate analogues useful as inhibitors of host-v5.-graft response
US6235887B1 (en) 1991-11-26 2001-05-22 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines
US5633360A (en) 1992-04-14 1997-05-27 Gilead Sciences, Inc. Oligonucleotide analogs capable of passive cell membrane permeation
US5434257A (en) 1992-06-01 1995-07-18 Gilead Sciences, Inc. Binding compentent oligomers containing unsaturated 3',5' and 2',5' linkages
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5721138A (en) 1992-12-15 1998-02-24 Sandford University Apolipoprotein(A) promoter and regulatory sequence constructs and methods of use
US5476925A (en) 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
GB9304618D0 (en) 1993-03-06 1993-04-21 Ciba Geigy Ag Chemical compounds
WO1994022891A1 (en) 1993-03-31 1994-10-13 Sterling Winthrop Inc. Oligonucleotides with amide linkages replacing phosphodiester linkages
US5625050A (en) 1994-03-31 1997-04-29 Amgen Inc. Modified oligonucleotides and intermediates useful in nucleic acid therapeutics
US5541110A (en) 1994-05-17 1996-07-30 Bristol-Myers Squibb Cloning and expression of a gene encoding bryodin 1 from Bryonia dioica
US5807718A (en) 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
WO1999056759A1 (en) 1998-05-07 1999-11-11 University Of South Florida Bone marrow cells as a source of neurons for brain and spinal cord repair
US6348185B1 (en) 1998-06-20 2002-02-19 Washington University School Of Medicine Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy
US6303374B1 (en) 2000-01-18 2001-10-16 Isis Pharmaceuticals Inc. Antisense modulation of caspase 3 expression
GB0001309D0 (en) 2000-01-20 2000-03-08 Nestle Sa Valve arrangement
EP2270146A1 (en) 2002-02-06 2011-01-05 Sanbio, Inc. Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene
US20050058641A1 (en) 2002-05-22 2005-03-17 Siemionow Maria Z. Tolerance induction and maintenance in hematopoietic stem cell allografts
ES2702942T3 (es) 2003-04-17 2019-03-06 Alnylam Pharmaceuticals Inc Agentes de ARNi modificados
EP1506997A1 (en) * 2003-08-14 2005-02-16 NeuroProgen GmbH Leipzig Method of generating neural stem cells
US20050182005A1 (en) 2004-02-13 2005-08-18 Tuschl Thomas H. Anti-microRNA oligonucleotide molecules
AU2005244154C1 (en) 2004-04-28 2010-07-01 Fibrogen, Inc. Treatments for pancreatic cancer
EP1799810A2 (en) 2004-10-12 2007-06-27 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
EP2314688B1 (en) 2004-11-12 2014-07-16 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
DE602005014537D1 (de) * 2005-03-23 2009-07-02 Stiftung Caesar Neurale Stammzellen
ES2524996T3 (es) 2005-06-16 2014-12-16 Ramot At Tel Aviv University Ltd. Células aisladas y poblaciones que comprenden a las mismas para el tratamiento de enfermedades del SNC
KR101022401B1 (ko) 2005-09-29 2011-03-15 아주대학교산학협력단 자살유전자를 발현하는 중간엽 줄기세포를 포함하는 암치료용 조성물
WO2007066338A1 (en) 2005-12-08 2007-06-14 Ramot At Tel Aviv University Ltd. Isolated oligodendrocyte-like cells and populations comprising same for the treatment of cns diseases
EP2064319B1 (en) 2006-08-28 2017-02-22 Yeda Research and Development Co. Ltd. Methods of generating glial and neuronal cells and use of same for the treatment of medical conditions of the cns
US20080176328A1 (en) * 2007-01-19 2008-07-24 Seoul National University Industry Foundation Method of inducing differentiation of mesenchymal stem cells into neurons
US8580757B2 (en) * 2007-08-09 2013-11-12 Thermo Fisher Scientific Biosciences Inc. Methods of modulating mesenchymal stem cell differentiation
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
EP2268797A1 (en) * 2008-03-31 2011-01-05 Hadasit Medical Research Services & Development Limited Motor neurons developed from stem cells
DK2285951T3 (en) 2008-05-28 2018-10-22 Univ Ramot TREATMENT OF CNS DISEASES OF CNS DISEASES
CA2756670A1 (en) 2009-03-26 2010-09-30 The Regents Of The University Of California Mesenchymal stem cells producing inhibitory rna for disease modification
US8852940B2 (en) * 2009-04-01 2014-10-07 The Regents Of The University Of California Embryonic stem cell specific microRNAs promote induced pluripotency
WO2011030336A1 (en) 2009-09-08 2011-03-17 Ramot At Tel-Aviv University Ltd. Methods of diagnosing amyotrophic lateral sclerosis (als)
WO2011159075A2 (ko) * 2010-06-14 2011-12-22 차의과학대학교 산학협력단 2차원 배양을 이용한 성체줄기세포의 신경전구세포로의 분화방법 및 신경전구세포를 이용한 신경손상 질환 치료용 약학 조성물
KR101183620B1 (ko) 2010-06-18 2012-09-18 서울대학교산학협력단 지방조직 유래의 간엽줄기세포를 포함하는 파킨슨병 진단용 조성물 및 파킨슨병 진단용 바이오마커
US9987309B2 (en) * 2010-07-08 2018-06-05 Duke University Direct reprogramming of cells to cardiac myocyte fate
US9783781B2 (en) 2010-08-16 2017-10-10 Exostem Biotec Ltd. Methods of generating oligodendrocytes and cell populations comprising same
JP2015509366A (ja) 2012-02-22 2015-03-30 ブレインステム バイオテック リミテッド 神経幹細胞および運動ニューロンの生成
US10034902B2 (en) 2012-02-22 2018-07-31 Exostem Biotec Ltd. MicroRNAs for the generation of astrocytes
JP2015510401A (ja) 2012-02-22 2015-04-09 ブレインステム バイオテック リミテッド ヒト疾患のインビトロモデル化および細胞に基づく治療のための間葉系幹細胞ならびにそのバンク

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144698A2 (en) * 2009-06-10 2010-12-16 Henry Ford Health System Methods, systems, and compositions for neuronal differentiation of multipotent stromal cells

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017115789A1 (ja) * 2015-12-28 2017-07-06 国立研究開発法人理化学研究所 加齢による生理機能の低下を回復または改善する組成物
JPWO2017115789A1 (ja) * 2015-12-28 2018-10-18 国立研究開発法人理化学研究所 加齢による生理機能の低下を回復または改善する組成物
US11041006B2 (en) 2015-12-28 2021-06-22 Riken Compositions for use in recovering or ameliorating deterioration of physiological functions due to aging
JP2019509065A (ja) * 2016-03-16 2019-04-04 サイナータ セラピューティクス リミテッド コロニー形成培地及びその使用
JP7048977B2 (ja) 2016-03-16 2022-04-06 サイナータ セラピューティクス リミテッド コロニー形成培地及びその使用
JP2019529425A (ja) * 2016-09-19 2019-10-17 フォンダツィオーネ・イスティトゥート・イタリアーノ・ディ・テクノロジャＦｏｎｄａｚｉｏｎｅ Ｉｓｔｉｔｕｔｏ Ｉｔａｌｉａｎｏ Ｄｉ Ｔｅｃｎｏｌｏｇｉａ ｍｉＲＮＡ医薬組成物およびその治療的使用
WO2023238948A1 (ja) * 2022-06-09 2023-12-14 国立大学法人九州大学 ｍｉＲＮＡを含む新規脳神経新生促進剤

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