JP2015507927A5 - - Google Patents
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- Publication number
- JP2015507927A5 JP2015507927A5 JP2014557124A JP2014557124A JP2015507927A5 JP 2015507927 A5 JP2015507927 A5 JP 2015507927A5 JP 2014557124 A JP2014557124 A JP 2014557124A JP 2014557124 A JP2014557124 A JP 2014557124A JP 2015507927 A5 JP2015507927 A5 JP 2015507927A5
- Authority
- JP
- Japan
- Prior art keywords
- endonuclease
- enzymatically active
- active fragment
- sample
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 21
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 21
- 239000012634 fragment Substances 0.000 claims 17
- 238000000034 method Methods 0.000 claims 16
- 235000018102 proteins Nutrition 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 102000004533 Endonucleases Human genes 0.000 claims 4
- 108010042407 Endonucleases Proteins 0.000 claims 4
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 4
- 102000040430 polynucleotide Human genes 0.000 claims 4
- 239000002157 polynucleotide Substances 0.000 claims 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 2
- 230000000996 additive effect Effects 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 230000003197 catalytic effect Effects 0.000 claims 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 2
- 235000013922 glutamic acid Nutrition 0.000 claims 2
- 239000004220 glutamic acid Substances 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 2
- JPSHPWJJSVEEAX-NFJMKROFSA-N (2s)-2-amino-4-fluoropentanedioic acid Chemical compound OC(=O)[C@@H](N)CC(F)C(O)=O JPSHPWJJSVEEAX-NFJMKROFSA-N 0.000 claims 1
- OGMADIBCHLQMIP-UHFFFAOYSA-N 2-aminoethanethiol;hydron;chloride Chemical compound Cl.NCCS OGMADIBCHLQMIP-UHFFFAOYSA-N 0.000 claims 1
- 241000122170 Aliivibrio salmonicida Species 0.000 claims 1
- OYIFNHCXNCRBQI-SCSAIBSYSA-N D-2-aminoadipic acid Chemical compound OC(=O)[C@H](N)CCCC(O)=O OYIFNHCXNCRBQI-SCSAIBSYSA-N 0.000 claims 1
- 238000001712 DNA sequencing Methods 0.000 claims 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 1
- 101710096438 DNA-binding protein Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 1
- GHAZCVNUKKZTLG-UHFFFAOYSA-N N-ethyl-succinimide Natural products CCN1C(=O)CCC1=O GHAZCVNUKKZTLG-UHFFFAOYSA-N 0.000 claims 1
- HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 claims 1
- 238000003559 RNA-seq method Methods 0.000 claims 1
- 241000607626 Vibrio cholerae Species 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 235000003704 aspartic acid Nutrition 0.000 claims 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- UHBYWPGGCSDKFX-UHFFFAOYSA-N carboxyglutamic acid Chemical compound OC(=O)C(N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-UHFFFAOYSA-N 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 229940118696 vibrio cholerae Drugs 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1202768.6A GB201202768D0 (en) | 2012-02-17 | 2012-02-17 | Endonucleases |
| GB1202768.6 | 2012-02-17 | ||
| GB1216029.7 | 2012-09-07 | ||
| GBGB1216029.7A GB201216029D0 (en) | 2012-09-07 | 2012-09-07 | Endonucleases |
| PCT/GB2013/050387 WO2013121228A1 (en) | 2012-02-17 | 2013-02-18 | Endonucleases |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017198348A Division JP2018038414A (ja) | 2012-02-17 | 2017-10-12 | エンドヌクレアーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015507927A JP2015507927A (ja) | 2015-03-16 |
| JP2015507927A5 true JP2015507927A5 (enExample) | 2017-03-09 |
| JP6259775B2 JP6259775B2 (ja) | 2018-01-10 |
Family
ID=47741183
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557124A Active JP6259775B2 (ja) | 2012-02-17 | 2013-02-18 | エンドヌクレアーゼ |
| JP2017198348A Pending JP2018038414A (ja) | 2012-02-17 | 2017-10-12 | エンドヌクレアーゼ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017198348A Pending JP2018038414A (ja) | 2012-02-17 | 2017-10-12 | エンドヌクレアーゼ |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9422595B2 (enExample) |
| EP (1) | EP2814954B1 (enExample) |
| JP (2) | JP6259775B2 (enExample) |
| KR (1) | KR102111178B1 (enExample) |
| CN (1) | CN104245928B (enExample) |
| AU (1) | AU2013220102A1 (enExample) |
| CA (1) | CA2863756C (enExample) |
| DK (1) | DK2814954T3 (enExample) |
| ES (1) | ES2622906T3 (enExample) |
| IN (1) | IN2014MN01822A (enExample) |
| LT (1) | LT2814954T (enExample) |
| WO (1) | WO2013121228A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| US11246905B2 (en) | 2016-08-15 | 2022-02-15 | President And Fellows Of Harvard College | Treating infections using IdsD from Proteus mirabilis |
| CN109207563A (zh) * | 2017-06-30 | 2019-01-15 | 青岛科技大学 | 一种lamp防污染的方法 |
| US20220251146A1 (en) * | 2019-05-06 | 2022-08-11 | President And Fellows Of Harvard College | Dna-degrading proteins and uses thereof |
| CN112176028B (zh) * | 2019-07-05 | 2024-04-16 | 深圳华大生命科学研究院 | 一种基于核酸内切酶的快速wgs建库方法 |
| PL431144A1 (pl) * | 2019-09-13 | 2021-03-22 | Blirt Spółka Akcyjna | Nowa, niespecyficzna termolabilna nukleaza aktywna w niskiej temperaturze, szerokim zakresie pH oraz wysokich stężeniach soli |
| EP4347868A1 (en) | 2021-05-27 | 2024-04-10 | Lumiradx Uk Ltd | Improvements in or relating to digestion of reaction products |
| US20250092372A1 (en) | 2022-01-20 | 2025-03-20 | C-Lecta Gmbh | Nuclease having improved salt tolerance and/or temperature performance |
| CN119351373A (zh) * | 2023-07-24 | 2025-01-24 | 北京志道生物科技有限公司 | 一种核酸内切酶及其应用 |
| CN118165957B (zh) * | 2024-03-18 | 2025-06-13 | 北京石油化工学院 | 一种重组核酸酶及其应用 |
| WO2025227034A1 (en) * | 2024-04-26 | 2025-10-30 | New England Biolabs, Inc. | Salt active nuclease compositions and methods |
| CN120173915B (zh) * | 2025-05-21 | 2025-08-26 | 翌圣生物科技(上海)股份有限公司 | 一种全能核酸酶突变体及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5006472A (en) | 1988-06-03 | 1991-04-09 | Miles Inc. | Enzymatic purification process |
| US5700672A (en) | 1992-07-23 | 1997-12-23 | Stratagene | Purified thermostable pyrococcus furiousus DNA ligase |
| GB9716664D0 (en) | 1997-08-06 | 1997-10-15 | Norwegian Inst Of Fisheries & | A method of removing nucleic acid contamination reactions |
| US20020172972A1 (en) * | 2001-05-15 | 2002-11-21 | President And Fellows Of Harvard College | Use of a selectively inactivatable enzyme to digest contaminating nucleic acid |
| DE102005009479A1 (de) * | 2005-03-02 | 2006-09-07 | Molzym Gmbh & Co. Kg | Verwendung von Nukleasen zum Abbau von Nukleinsäure in Gegenwart von chaotropen Agenzien und/oder Tensiden |
| WO2006095769A1 (ja) * | 2005-03-08 | 2006-09-14 | Takara Bio Inc. | 低温性微生物由来エンドヌクレアーゼ |
| US20080044851A1 (en) | 2006-06-02 | 2008-02-21 | Epicentre Technologies | Compositions and methods for removal of DNA from a sample |
| WO2007142306A1 (ja) | 2006-06-08 | 2007-12-13 | Agave Inc. | 高水溶性アガベ・イヌリン、アガベ・イヌリン含有製品、アガベ・イヌリン由来製品、副産物及びそれらの製法 |
| WO2009143500A2 (en) | 2008-05-23 | 2009-11-26 | Life Technologies Corporation | Methods for removing nucleic acid contamination from reagents |
| GB2474225A (en) * | 2009-07-21 | 2011-04-13 | Biotec Pharmacon Asa | DNase for decontamination of reverse transcription and amplification reactions |
-
2013
- 2013-02-18 KR KR1020147024472A patent/KR102111178B1/ko active Active
- 2013-02-18 ES ES13705249.4T patent/ES2622906T3/es active Active
- 2013-02-18 JP JP2014557124A patent/JP6259775B2/ja active Active
- 2013-02-18 EP EP13705249.4A patent/EP2814954B1/en active Active
- 2013-02-18 IN IN1822MUN2014 patent/IN2014MN01822A/en unknown
- 2013-02-18 CA CA2863756A patent/CA2863756C/en active Active
- 2013-02-18 CN CN201380009915.2A patent/CN104245928B/zh active Active
- 2013-02-18 WO PCT/GB2013/050387 patent/WO2013121228A1/en not_active Ceased
- 2013-02-18 AU AU2013220102A patent/AU2013220102A1/en not_active Abandoned
- 2013-02-18 LT LTEP13705249.4T patent/LT2814954T/lt unknown
- 2013-02-18 DK DK13705249.4T patent/DK2814954T3/en active
- 2013-02-18 US US14/377,013 patent/US9422595B2/en active Active
-
2016
- 2016-07-15 US US15/211,843 patent/US9650618B2/en active Active
-
2017
- 2017-10-12 JP JP2017198348A patent/JP2018038414A/ja active Pending
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