IN2014MN01822A - - Google Patents

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Publication number
IN2014MN01822A
IN2014MN01822A IN1822MUN2014A IN2014MN01822A IN 2014MN01822 A IN2014MN01822 A IN 2014MN01822A IN 1822MUN2014 A IN1822MUN2014 A IN 1822MUN2014A IN 2014MN01822 A IN2014MN01822 A IN 2014MN01822A
Authority
IN
India
Prior art keywords
endonuclease
sequence
residue
seq
enzymes
Prior art date
Application number
Inventor
Olav Lanes
Linda Havdalen
Terese Solstad
Marit Lorentzen
Bjørn Altermark
Ingar Leiros
Ronny Helland
Original Assignee
Biotec Pharmacon Asa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1202768.6A external-priority patent/GB201202768D0/en
Priority claimed from GBGB1216029.7A external-priority patent/GB201216029D0/en
Application filed by Biotec Pharmacon Asa filed Critical Biotec Pharmacon Asa
Publication of IN2014MN01822A publication Critical patent/IN2014MN01822A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Abstract

The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.
IN1822MUN2014 2012-02-17 2013-02-18 IN2014MN01822A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1202768.6A GB201202768D0 (en) 2012-02-17 2012-02-17 Endonucleases
GBGB1216029.7A GB201216029D0 (en) 2012-09-07 2012-09-07 Endonucleases
PCT/GB2013/050387 WO2013121228A1 (en) 2012-02-17 2013-02-18 Endonucleases

Publications (1)

Publication Number Publication Date
IN2014MN01822A true IN2014MN01822A (en) 2015-07-03

Family

ID=47741183

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1822MUN2014 IN2014MN01822A (en) 2012-02-17 2013-02-18

Country Status (12)

Country Link
US (2) US9422595B2 (en)
EP (1) EP2814954B1 (en)
JP (2) JP6259775B2 (en)
KR (1) KR102111178B1 (en)
CN (1) CN104245928B (en)
AU (1) AU2013220102A1 (en)
CA (1) CA2863756C (en)
DK (1) DK2814954T3 (en)
ES (1) ES2622906T3 (en)
IN (1) IN2014MN01822A (en)
LT (1) LT2814954T (en)
WO (1) WO2013121228A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
US11246905B2 (en) 2016-08-15 2022-02-15 President And Fellows Of Harvard College Treating infections using IdsD from Proteus mirabilis
CN109207563A (en) * 2017-06-30 2019-01-15 青岛科技大学 A kind of antipollution method of LAMP
WO2020227415A1 (en) * 2019-05-06 2020-11-12 President And Fellows Of Harvard College Dna-degrading proteins and uses thereof
CN112176028B (en) * 2019-07-05 2024-04-16 深圳华大生命科学研究院 Rapid WGS library construction method based on endonuclease
PL431144A1 (en) 2019-09-13 2021-03-22 Blirt Spółka Akcyjna New non-specific thermolabile nuclease, active in low temperatures, with a wide pH range and high salt concentrations
EP4347868A1 (en) * 2021-05-27 2024-04-10 Lumiradx Uk Ltd Improvements in or relating to digestion of reaction products
WO2023139240A1 (en) 2022-01-20 2023-07-27 C-Lecta Gmbh Nuclease having improved salt tolerance and/or temperature performance
CN116640743B (en) * 2023-07-24 2023-11-07 北京志道生物科技有限公司 Endonuclease and application thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5006472A (en) 1988-06-03 1991-04-09 Miles Inc. Enzymatic purification process
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US20020172972A1 (en) 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
DE102005009479A1 (en) 2005-03-02 2006-09-07 Molzym Gmbh & Co. Kg Use of nucleases for the degradation of nucleic acid in the presence of chaotropic agents and / or surfactants
WO2006095769A1 (en) * 2005-03-08 2006-09-14 Takara Bio Inc. Microorganism-derived psychrophilic endonuclease
WO2007143206A2 (en) 2006-06-02 2007-12-13 Epicentre Technologies Compositions and methods for removal of dna from a sample
MX2008015689A (en) 2006-06-08 2009-07-03 Agave Inc Highly water-soluble agave inulin, agave inulin-containing product, agave inulin-origin product and by product and method of producing the same.
CN102112627A (en) 2008-05-23 2011-06-29 生命技术公司 Methods for removing nucleic acid contamination from reagents
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions

Also Published As

Publication number Publication date
KR20140135962A (en) 2014-11-27
JP2018038414A (en) 2018-03-15
CN104245928B (en) 2017-08-18
CA2863756A1 (en) 2013-08-22
EP2814954B1 (en) 2017-04-05
CN104245928A (en) 2014-12-24
JP6259775B2 (en) 2018-01-10
EP2814954A1 (en) 2014-12-24
LT2814954T (en) 2017-05-10
CA2863756C (en) 2018-03-06
KR102111178B1 (en) 2020-05-15
WO2013121228A1 (en) 2013-08-22
US9650618B2 (en) 2017-05-16
ES2622906T3 (en) 2017-07-07
US20160355798A1 (en) 2016-12-08
US20140370514A1 (en) 2014-12-18
AU2013220102A1 (en) 2014-08-21
JP2015507927A (en) 2015-03-16
DK2814954T3 (en) 2017-05-15
US9422595B2 (en) 2016-08-23

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