IN2014DE00902A - - Google Patents
Download PDFInfo
- Publication number
- IN2014DE00902A IN2014DE00902A IN902DE2014A IN2014DE00902A IN 2014DE00902 A IN2014DE00902 A IN 2014DE00902A IN 902DE2014 A IN902DE2014 A IN 902DE2014A IN 2014DE00902 A IN2014DE00902 A IN 2014DE00902A
- Authority
- IN
- India
- Prior art keywords
- recombinant
- ssopox
- nanobiocatalyst
- enzymes
- organophosphate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
- A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
- A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
- A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/08—Phosphoric triester hydrolases (3.1.8)
- C12Y301/08001—Aryldialkylphosphatase (3.1.8.1), i.e. paraoxonase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/08—Nanoparticles or nanotubes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Inorganic Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The present disclosure provides novel polynucleotide sequences for high level expression in Escherichia coli and encoding recombinant SsoPox wild-type enzyme and its variant having enhanced organophosphate (OP)-hydrolyzing activity. The invention also provides a novel method to produce the recombinant enzymes in highly pure and active form in an unprecedented high yield. The invention also provide a method to generate reusable nanobiocatalyst by covalently immobilizing pure recombinant SsoPox enzymes onto magnetic nanoparticles. The reusable nanobiocatalyst can be recovered from the reaction mixture after its use and can be stored and reused multiple times to degrade the organophosphate-compounds. The recombinant SsoPox enzymes and rSsoPox-immobilized nanobiocatalyst can be used as an economical, effective and environmental-friendly OPcompound biodegrading agent.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN902DE2014 IN2014DE00902A (en) | 2014-03-28 | 2014-09-18 | |
PCT/IB2014/064616 WO2015145222A2 (en) | 2014-03-28 | 2014-09-18 | Recombinant and stable ssopox enzymes, method of generation thereof and reusable nanobiocatalyst of the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN902DE2014 IN2014DE00902A (en) | 2014-03-28 | 2014-09-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014DE00902A true IN2014DE00902A (en) | 2015-10-02 |
Family
ID=54196509
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN902DE2014 IN2014DE00902A (en) | 2014-03-28 | 2014-09-18 |
Country Status (2)
Country | Link |
---|---|
IN (1) | IN2014DE00902A (en) |
WO (1) | WO2015145222A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9597672B2 (en) | 2011-03-10 | 2017-03-21 | Cornell University | Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use |
US10881102B2 (en) | 2015-05-18 | 2021-01-05 | Zymtronix, Llc | Magnetically immobilized microbiocidal enzymes |
WO2017011292A1 (en) | 2015-07-15 | 2017-01-19 | Zymtronix, Llc | Automated bionanocatalyst production |
CA3031802A1 (en) | 2016-08-13 | 2018-02-22 | Zymtronix Catalytic Systems, Inc. | Magnetically immobilized biocidal enzymes and biocidal chemicals |
SE1750143A1 (en) | 2017-02-15 | 2018-08-16 | Pharem Biotech Ab | Enzyme identification |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2915489B1 (en) * | 2007-04-27 | 2009-07-31 | Univ Henri Poincare Nancy I Et | MUTANT HYPERTHERMOPHILIC PHOSPHOTRIESTERASES AND USES THEREOF |
CA2895901A1 (en) * | 2012-12-20 | 2014-06-26 | Olivier-Georges BOUMENDIL | Enzyme having a nmda receptor antagonist activity and/or an anticholinergic activity |
-
2014
- 2014-09-18 IN IN902DE2014 patent/IN2014DE00902A/en unknown
- 2014-09-18 WO PCT/IB2014/064616 patent/WO2015145222A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2015145222A3 (en) | 2016-07-14 |
WO2015145222A2 (en) | 2015-10-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX2020010841A (en) | Detergent composition. | |
MX2016011752A (en) | Hppd variants and methods of use. | |
IN2014DE00902A (en) | ||
MY171604A (en) | Polypeptide for hydrolytic cleavage of zearalenone and/or zearalenone derivatives, isolated polynucleotide thereof and additive containing polypeptide, use of said polypeptide and method | |
MX2016011745A (en) | Hppd variants and methods of use. | |
EP3741849A3 (en) | Protease variants and polynucleotides encoding same | |
MX2017007731A (en) | Detergent compositions, lipase variants and polynucleotides encoding same. | |
MX2021014663A (en) | Compound targeting il-23a and tnf-alpha and uses thereof. | |
MX2018010288A (en) | Transposon system and methods of use. | |
MX2018007352A (en) | Modulators of complement activity. | |
MX355855B (en) | Method for the control of odour. | |
IN2015DN00849A (en) | ||
MX2017007105A (en) | Lipase variants and polynucleotides encoding same. | |
PH12018500155A1 (en) | Recombinant orf virus vector | |
MX2017007554A (en) | Detergents and cleaning agents comprising a combination of amylase and protease. | |
MX2021013879A (en) | Lipase variants and polynucleotides encoding same. | |
WO2015168617A3 (en) | Compositions and methods for modulating mtorc1 | |
MY181148A (en) | Microorganisms for producing diamine and process for producing diamine using them | |
MY178471A (en) | Microorganisms for producing diamine and process for producing diamine using them | |
PH12017502345A1 (en) | Novel thrombin inhibitors | |
PH12014502227A1 (en) | Plants having one or more enhanced yield-related traits and method for making the same | |
MX2018014301A (en) | Hppd variants and methods of use. | |
MX2018003040A (en) | Methods for treating relapsing forms of multiple sclerosis. | |
PH12015501466A1 (en) | Plants having enhanced yield-related traits and method for making the same | |
WO2016083735A8 (en) | Method for metathesis of a fatty acid or fatty acids |