JP2015006203A - Pcr反応をリアルタイムで監視するための光検出システム - Google Patents
Pcr反応をリアルタイムで監視するための光検出システム Download PDFInfo
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Abstract
【解決手段】本発明は、複数の試料チャンバ(101−104)中のPCR反応を複数の光学ユニット(106,107)によってリアルタイムで監視する光検出システムに関する。各光学ユニットが各試料チャンバに対して相対運動することにより、色多重の効果と空間多重の効果とが組み合わされ、PCR反応の期間内で試料中の病原体を光学的に検出して定量結果を出力することができる。
【選択図】図1
Description
本願は、2009年4月15日付欧州出願EP09157910.2の優先権を主張する。この出願の内容は参照によりすべて本願に組み込まれるものとする。
本発明は光検出システムに関する。本発明は、特に、少なくとも2つの異なる試料チャンバ中の複数の試料成分を検出する光多重システム、少なくとも2つの異なる試料チャンバ中の複数の試料成分を検出する方法、コンピュータプログラムエレメント、ならびに、コンピュータで読み出し可能な媒体に関する。
ポリメラーゼ連鎖反応PCRは分子生物学の分野で広汎に利用されている技術である。この技術の名称は、鍵となる1つの成分、すなわち、試験管内の酵素複製によってDNA片を増幅するデオキシリボ核酸DNAのポリメラーゼに由来している。PCRが進行すると、形成されたDNAは複製のテンプレートとして用いられる。これにより、DNAテンプレートが指数的に増幅されるという連鎖反応が生じる。PCRにより、1個または数個のみのDNA片を数オーダーの規模で増幅して、数100万個以上のDNA片の複製を得ることができる。なお、PCRは、遺伝子操作技術の種々の手法を実行するために集中的に修正可能である。
本発明の課題は、試料成分を検出する手法を改善することである。
試料チャンバ:本発明においては、試料、特に液体試料を収容できるものであれば、カートリッジ、チューブ、コンテナなどのいずれの形態の容器であっても、“試料チャンバ”と称する。特に、PCRチャンバとしてのカートリッジ、チューブ、コンテナは、所望の透光性を有するか、あるいは、ポリプロピレンまたはその他の熱可塑性ポリマーなどの材料から形成され、“試料チャンバ”に含まれる概念として用いられる。
以下に、本発明の特徴および利点を図示の実施例に則して詳細に説明する。ただし、実施例は説明のためのものであり、本発明を限定しない。
Claims (1)
- 少なくとも2つの試料チャンバ(101−104)中の複数の試料成分を検出する光多重システム(100)であって、
前記光多重システムは、
第1の光源(108)および第1の検出器(109)を含む第1の光学ユニット(106)であって、前記第1の光学ユニット(106)は、第1のポリメラーゼ連鎖反応(PCR)の生成物の検出を行う、第1の光学ユニット(106)と、
第2の光源(110)および第2の検出器(111)を含む第2の光学ユニット(107)であって、前記第2の光学ユニット(107)は、第2のポリメラーゼ連鎖反応(PCR)の生成物の検出を行い、前記第2の光学ユニットは、前記第1の光学ユニットと空間的に相互に離隔されている、第2の光学ユニット(107)と、
前記少なくとも2つの試料チャンバの第1の試料チャンバと関連する第1のヒータであって、前記第1のヒータは、前記第1の試料チャンバにおいて第1の温度経過を形成する、第1のヒータと、
前記少なくとも2つの試料チャンバの第2の試料チャンバと関連する第2のヒータであって、前記第2のヒータは、前記第2の試料チャンバにおいて第2の温度経過を形成する、第2のヒータと、
前記第1の光学ユニット(106)および前記第2の光学ユニット(107)が固定されている回転フレームと、
前記第1の光学ユニット(106)および前記第2の光学ユニット(107)が、第1の位置と第2の位置との間を移動するように前記回転フレームを回転させるように構成されたモータと、
PCRプロトコルを受け取る制御ユニットと
を備えており、
前記第1の位置にあるときに、前記第1の光源(108)が前記第1の試料チャンバを照明し、かつ、前記第1の検出器(109)が前記第1の試料チャンバからの光を受け取るように、前記第1の光学ユニット(106)が、前記第1の試料チャンバおよび前記第1のヒータに対して配置され、一方で、前記第2の光源(110)が前記第2の試料チャンバを照明し、かつ、前記第2の検出器(111)が前記第2の試料チャンバからの光を受け取るように、前記第2の光学ユニット(107)が、前記第2の試料チャンバおよび前記第2のヒータに対して配置され、
前記第1の光学ユニット(106)および前記第2の光学ユニット(107)は、前記少なくとも2つの試料チャンバを同時に照明し、前記第1の検出器(109)および前記第2の検出器(111)は、前記少なくとも2つの試料チャンバからの光を同時に受け取り、ここで、前記第1の光学ユニット(106)および前記第2の光学ユニット(107)は、前記少なくとも2つの試料チャンバにおいて、2つの異なる光波長を用いて、第1の光学測定および第2の光学測定を同時に行い、
前記制御ユニットは、前記PCRプロトコルにしたがって、前記第1の試料チャンバおよび前記第2の試料チャンバにおいてリアルタイムPCR反応を生じさせるように、前記第1のヒータおよび前記第2のヒータを制御し、前記制御ユニットは、前記少なくとも2つの試料チャンバに対して、前記第1の光学ユニットおよび前記第2の光学ユニットを相対運動させることにより増幅された目標DNA分子を同時に定量化する
ことを特徴とする光多重システム。
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EP09157910 | 2009-04-15 | ||
EP09157910.2 | 2009-04-15 |
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US (1) | US8441629B2 (ja) |
EP (1) | EP2419743B1 (ja) |
JP (2) | JP2012524242A (ja) |
KR (1) | KR20120012468A (ja) |
CN (1) | CN102341710B (ja) |
AU (1) | AU2010237532B2 (ja) |
BR (1) | BRPI1012522B1 (ja) |
CA (1) | CA2752760C (ja) |
ES (1) | ES2822105T3 (ja) |
RU (1) | RU2548606C2 (ja) |
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KR102111679B1 (ko) | 2018-04-18 | 2020-05-15 | 주식회사 창 헬스케어 | 핵산 분석 장치 및 방법 |
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EP2419743A1 (en) | 2012-02-22 |
BRPI1012522A2 (pt) | 2017-06-27 |
CA2752760C (en) | 2019-12-24 |
US8441629B2 (en) | 2013-05-14 |
RU2548606C2 (ru) | 2015-04-20 |
CN102341710A (zh) | 2012-02-01 |
US20120033210A1 (en) | 2012-02-09 |
EP2419743B1 (en) | 2020-08-05 |
KR20120012468A (ko) | 2012-02-10 |
CN102341710B (zh) | 2015-04-01 |
CA2752760A1 (en) | 2010-10-21 |
RU2011146151A (ru) | 2013-05-20 |
BRPI1012522B1 (pt) | 2019-11-26 |
JP2012524242A (ja) | 2012-10-11 |
ZA201105624B (en) | 2012-04-25 |
ES2822105T3 (es) | 2021-04-29 |
AU2010237532B2 (en) | 2014-11-20 |
JP6296955B2 (ja) | 2018-03-20 |
AU2010237532A1 (en) | 2011-08-18 |
WO2010118541A1 (en) | 2010-10-21 |
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