JP2014531196A5 - - Google Patents

Info

Publication number

JP2014531196A5

JP2014531196A5 JP2014522152A JP2014522152A JP2014531196A5 JP 2014531196 A5 JP2014531196 A5 JP 2014531196A5 JP 2014522152 A JP2014522152 A JP 2014522152A JP 2014522152 A JP2014522152 A JP 2014522152A JP 2014531196 A5 JP2014531196 A5 JP 2014531196A5

Authority

JP

Japan

Prior art keywords

polynucleotide

target polynucleotide

polymer

double

stranded

Prior art date

2012-07-25

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 108091033319 polynucleotide Proteins 0.000 claims description 133
• 102000040430 polynucleotide Human genes 0.000 claims description 133
• 239000002157 polynucleotide Substances 0.000 claims description 133
• 238000000034 method Methods 0.000 claims description 54
• 229920000642 polymer Polymers 0.000 claims description 38
• 239000011148 porous material Substances 0.000 claims description 38
• 230000000295 complement effect Effects 0.000 claims description 16
• 239000012528 membrane Substances 0.000 claims description 15
• 238000012163 sequencing technique Methods 0.000 claims description 15
• 108091008324 binding proteins Proteins 0.000 claims description 10
• 239000002773 nucleotide Substances 0.000 claims description 8
• 125000003729 nucleotide group Chemical group 0.000 claims description 8
• 108060004795 Methyltransferase Proteins 0.000 claims description 7
• 102000004169 proteins and genes Human genes 0.000 claims description 7
• 108090000623 proteins and genes Proteins 0.000 claims description 7
• 230000008878 coupling Effects 0.000 claims description 6
• 238000010168 coupling process Methods 0.000 claims description 6
• 238000005859 coupling reaction Methods 0.000 claims description 6
• 101710092462 Alpha-hemolysin Proteins 0.000 claims description 4
• 239000012530 fluid Substances 0.000 claims description 4
• 230000003993 interaction Effects 0.000 claims description 4
• 239000000463 material Substances 0.000 claims description 4
• 230000002194 synthesizing effect Effects 0.000 claims description 4
• 239000003550 marker Substances 0.000 claims description 3
• 239000007787 solid Substances 0.000 claims description 3
• 208000035657 Abasia Diseases 0.000 claims description 2
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 2
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 2
• 241000118313 Picovirinae Species 0.000 claims description 2
• 241000700605 Viruses Species 0.000 claims description 2
• 229920001184 polypeptide Polymers 0.000 claims description 2
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
• 239000000126 substance Chemical group 0.000 claims description 2
• 102000014914 Carrier Proteins Human genes 0.000 claims 4
• 108020004414 DNA Proteins 0.000 description 7
• 102000023732 binding proteins Human genes 0.000 description 6
• 230000000692 anti-sense effect Effects 0.000 description 5
• 108091081021 Sense strand Proteins 0.000 description 2
• 238000004132 cross linking Methods 0.000 description 2
• 125000005647 linker group Chemical group 0.000 description 2
• 230000010076 replication Effects 0.000 description 2
• 238000000926 separation method Methods 0.000 description 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
• 102000053602 DNA Human genes 0.000 description 1
• 239000000232 Lipid Bilayer Substances 0.000 description 1
• 230000001419 dependent effect Effects 0.000 description 1
• 102200026914 rs730882246 Human genes 0.000 description 1
• 102220072644 rs753744791 Human genes 0.000 description 1
• 230000007704 transition Effects 0.000 description 1