JP2014527072A5 - - Google Patents

Info

Publication number

JP2014527072A5

JP2014527072A5 JP2014529962A JP2014529962A JP2014527072A5 JP 2014527072 A5 JP2014527072 A5 JP 2014527072A5 JP 2014529962 A JP2014529962 A JP 2014529962A JP 2014529962 A JP2014529962 A JP 2014529962A JP 2014527072 A5 JP2014527072 A5 JP 2014527072A5

Authority

JP

Japan

Prior art keywords

core protein

seq

concentration

amino acid

item

Prior art date

2012-09-10

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims description 83
• 101710132601 Capsid protein Proteins 0.000 claims description 38
• 241000700721 Hepatitis B virus Species 0.000 claims description 38
• 210000000234 capsid Anatomy 0.000 claims description 33
• 239000000243 solution Substances 0.000 claims description 28
• 239000003814 drug Substances 0.000 claims description 26
• 150000001413 amino acids Chemical group 0.000 claims description 25
• 229940079593 drug Drugs 0.000 claims description 24
• 235000001014 amino acid Nutrition 0.000 claims description 22
• 239000003638 chemical reducing agent Substances 0.000 claims description 22
• 239000003398 denaturant Substances 0.000 claims description 19
• 239000004472 Lysine Substances 0.000 claims description 14
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 14
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 10
• 239000012895 dilution Substances 0.000 claims description 10
• 238000010790 dilution Methods 0.000 claims description 10
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 8
• WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 6
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 6
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
• 238000007865 diluting Methods 0.000 claims description 6
• 230000003612 virological effect Effects 0.000 claims description 6
• 239000003795 chemical substances by application Substances 0.000 claims description 5
• 239000004475 Arginine Substances 0.000 claims description 4
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
• PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims description 4
• RHQDFWAXVIIEBN-UHFFFAOYSA-N Trifluoroethanol Chemical compound OCC(F)(F)F RHQDFWAXVIIEBN-UHFFFAOYSA-N 0.000 claims description 4
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 4
• 210000004899 c-terminal region Anatomy 0.000 claims description 4
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 4
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 4
• 235000018417 cysteine Nutrition 0.000 claims description 4
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims description 4
• 238000009792 diffusion process Methods 0.000 claims description 4
• VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims description 4
• 229960003180 glutathione Drugs 0.000 claims description 4
• 229960000789 guanidine hydrochloride Drugs 0.000 claims description 4
• PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 4
• ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims description 4
• 238000012986 modification Methods 0.000 claims description 4
• 230000004048 modification Effects 0.000 claims description 4
• 108020004707 nucleic acids Proteins 0.000 claims description 4
• 102000039446 nucleic acids Human genes 0.000 claims description 4
• 150000007523 nucleic acids Chemical class 0.000 claims description 4
• 229920000642 polymer Polymers 0.000 claims description 4
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
• 235000018102 proteins Nutrition 0.000 claims description 4
• 108090000623 proteins and genes Proteins 0.000 claims description 4
• 102000004169 proteins and genes Human genes 0.000 claims description 4
• 150000003384 small molecules Chemical class 0.000 claims description 4
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 4
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
• VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 2
• 108010024636 Glutathione Proteins 0.000 claims description 2
• WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 2
• 230000015572 biosynthetic process Effects 0.000 claims description 2
• 239000004202 carbamide Substances 0.000 claims description 2
• UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims description 2
• 239000003937 drug carrier Substances 0.000 claims description 2
• 229910052744 lithium Inorganic materials 0.000 claims description 2
• 229960003151 mercaptamine Drugs 0.000 claims description 2
• 229940124597 therapeutic agent Drugs 0.000 claims description 2
• 239000000203 mixture Substances 0.000 claims 2
• 239000003607 modifier Substances 0.000 claims 2
• 230000001225 therapeutic effect Effects 0.000 claims 1