JP2014525735A5 - - Google Patents
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- Publication number
- JP2014525735A5 JP2014525735A5 JP2014516358A JP2014516358A JP2014525735A5 JP 2014525735 A5 JP2014525735 A5 JP 2014525735A5 JP 2014516358 A JP2014516358 A JP 2014516358A JP 2014516358 A JP2014516358 A JP 2014516358A JP 2014525735 A5 JP2014525735 A5 JP 2014525735A5
- Authority
- JP
- Japan
- Prior art keywords
- single variable
- variable domain
- immunoglobulin single
- protein
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108060003951 Immunoglobulin Proteins 0.000 claims 33
- 102000018358 immunoglobulin Human genes 0.000 claims 33
- 108091006027 G proteins Proteins 0.000 claims 15
- 102000030782 GTP binding Human genes 0.000 claims 15
- 108091000058 GTP-Binding Proteins 0.000 claims 15
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims 12
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims 12
- 229920001184 polypeptide Polymers 0.000 claims 7
- 102000004196 processed proteins & peptides Human genes 0.000 claims 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims 7
- 150000001413 amino acids Chemical class 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000003446 ligand Substances 0.000 claims 5
- 238000000034 method Methods 0.000 claims 5
- 102000005962 receptors Human genes 0.000 claims 5
- 108020003175 receptors Proteins 0.000 claims 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 150000007523 nucleic acids Chemical group 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 238000012216 screening Methods 0.000 claims 3
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims 2
- 238000010494 dissociation reaction Methods 0.000 claims 2
- 230000005593 dissociations Effects 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- XOFLBQFBSOEHOG-UUOKFMHZSA-N γS-GTP Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=S)[C@@H](O)[C@H]1O XOFLBQFBSOEHOG-UUOKFMHZSA-N 0.000 claims 2
- 102000006395 Globulins Human genes 0.000 claims 1
- 108010044091 Globulins Proteins 0.000 claims 1
- 102000003839 Human Proteins Human genes 0.000 claims 1
- 108090000144 Human Proteins Proteins 0.000 claims 1
- 108010003723 Single-Domain Antibodies Proteins 0.000 claims 1
- 239000000556 agonist Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 102000034345 heterotrimeric G proteins Human genes 0.000 claims 1
- 108091006093 heterotrimeric G proteins Proteins 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000007781 signaling event Effects 0.000 claims 1
- 230000000087 stabilizing effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161571159P | 2011-06-21 | 2011-06-21 | |
| US61/571,159 | 2011-06-21 | ||
| EP11181357.2 | 2011-09-15 | ||
| EP11181357 | 2011-09-15 | ||
| PCT/EP2012/062036 WO2012175643A2 (en) | 2011-06-21 | 2012-06-21 | Binding domains directed against gpcr:g protein complexes and uses derived thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017026632A Division JP2017127306A (ja) | 2011-06-21 | 2017-02-16 | Ｇｐｃｒ：ｇタンパク質複合体に対して作製された結合ドメインおよびそれに由来する使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014525735A JP2014525735A (ja) | 2014-10-02 |
| JP2014525735A5 true JP2014525735A5 (enExample) | 2015-08-13 |
| JP6164535B2 JP6164535B2 (ja) | 2017-07-19 |
Family
ID=47423019
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014516358A Active JP6164535B2 (ja) | 2011-06-21 | 2012-06-21 | Ｇｐｃｒ：ｇタンパク質複合体に対して作製された結合ドメインおよびそれに由来する使用 |
| JP2017026632A Pending JP2017127306A (ja) | 2011-06-21 | 2017-02-16 | Ｇｐｃｒ：ｇタンパク質複合体に対して作製された結合ドメインおよびそれに由来する使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017026632A Pending JP2017127306A (ja) | 2011-06-21 | 2017-02-16 | Ｇｐｃｒ：ｇタンパク質複合体に対して作製された結合ドメインおよびそれに由来する使用 |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US9695227B2 (enExample) |
| EP (3) | EP4282882A3 (enExample) |
| JP (2) | JP6164535B2 (enExample) |
| CN (2) | CN107857810A (enExample) |
| AU (1) | AU2012273987B2 (enExample) |
| CA (1) | CA2839834A1 (enExample) |
| DK (1) | DK2723764T3 (enExample) |
| ES (1) | ES2662372T3 (enExample) |
| HR (1) | HRP20180443T1 (enExample) |
| HU (1) | HUE036982T2 (enExample) |
| NO (1) | NO2723764T3 (enExample) |
| PL (1) | PL2723764T3 (enExample) |
| PT (1) | PT2723764T (enExample) |
| WO (1) | WO2012175643A2 (enExample) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201014715D0 (en) | 2010-09-06 | 2010-10-20 | Vib Vzw | Nanobodies stabilizing functional conformational states of GPCRS |
| ES2662372T3 (es) | 2011-06-21 | 2018-04-06 | Vib Vzw | Dominios de unión dirigidos contra complejos GPCR:proteína G y usos derivados de los mismos |
| EP2867674B1 (en) | 2012-06-28 | 2018-10-10 | UCB Biopharma SPRL | A method for identifying compounds of therapeutic interest |
| US9409139B2 (en) | 2013-08-05 | 2016-08-09 | Twist Bioscience Corporation | De novo synthesized gene libraries |
| US10174115B2 (en) | 2014-07-04 | 2019-01-08 | INSERM (Institut National de la Santé et de la Recherche Médicale | Anti-mGluR2 conformational single domain antibodies and uses thereof |
| US20170316147A1 (en) | 2014-10-27 | 2017-11-02 | King Abdullah University Of Science And Technology | Methods and systems for identifying ligand-protein binding sites |
| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| AU2016324296A1 (en) | 2015-09-18 | 2018-04-12 | Twist Bioscience Corporation | Oligonucleic acid variant libraries and synthesis thereof |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| CN108603307A (zh) | 2015-12-01 | 2018-09-28 | 特韦斯特生物科学公司 | 功能化表面及其制备 |
| CN105802970A (zh) * | 2016-05-30 | 2016-07-27 | 东北师范大学 | 靶向沉默Gβ1的shRNA |
| CA3034769A1 (en) | 2016-08-22 | 2018-03-01 | Twist Bioscience Corporation | De novo synthesized nucleic acid libraries |
| CN110248724B (zh) | 2016-09-21 | 2022-11-18 | 特韦斯特生物科学公司 | 基于核酸的数据存储 |
| CN107037116B (zh) * | 2016-12-01 | 2019-04-23 | 白波 | 检测gpcr之间相互作用及其交界面的方法 |
| CN110366613A (zh) | 2016-12-16 | 2019-10-22 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| WO2018156792A1 (en) | 2017-02-22 | 2018-08-30 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CN110913865A (zh) | 2017-03-15 | 2020-03-24 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| US11407837B2 (en) * | 2017-09-11 | 2022-08-09 | Twist Bioscience Corporation | GPCR binding proteins and synthesis thereof |
| KR102637566B1 (ko) | 2017-10-20 | 2024-02-16 | 트위스트 바이오사이언스 코포레이션 | 폴리뉴클레오타이드 합성을 위한 가열된 나노웰 |
| WO2019086548A1 (en) | 2017-10-31 | 2019-05-09 | Vib Vzw | Novel antigen-binding chimeric proteins and methods and uses thereof |
| JP7343488B2 (ja) * | 2017-12-21 | 2023-09-12 | フイルメニツヒ ソシエテ アノニム | 匂い物質受容体のためのポジティブなアロステリックモジュレーターを同定するための方法 |
| CN112041438B (zh) | 2018-01-04 | 2025-05-23 | 特韦斯特生物科学公司 | 基于dna的数字信息存储 |
| WO2019155041A1 (en) * | 2018-02-12 | 2019-08-15 | Vib Vzw | Gβγ COMPLEX ANTIBODIES AND USES THEREOF |
| CN108586620B (zh) * | 2018-05-10 | 2021-11-23 | 山东大学 | 筛选昆虫性信息素受体的配体的传感器分子以及筛选方法 |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| FR3084365B1 (fr) * | 2018-07-27 | 2020-10-23 | Cisbio Bioassays | Anticorps a domaine unique qui se lient a la proteine g alpha |
| CA3122045A1 (en) * | 2018-12-21 | 2020-06-25 | Vib Vzw | Fusion proteins comprising a cytokine and scaffold protein |
| CA3124980A1 (en) | 2018-12-26 | 2020-07-02 | Twist Bioscience Corporation | Highly accurate de novo polynucleotide synthesis |
| FR3092172B1 (fr) * | 2019-01-30 | 2021-02-12 | Cisbio Bioassays | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
| KR20210143766A (ko) | 2019-02-26 | 2021-11-29 | 트위스트 바이오사이언스 코포레이션 | Glp1 수용체에 대한 변이체 핵산 라이브러리 |
| CN113785057A (zh) | 2019-02-26 | 2021-12-10 | 特韦斯特生物科学公司 | 用于抗体优化的变异核酸文库 |
| SG11202111980QA (en) * | 2019-04-29 | 2021-11-29 | Confo Therapeutics N V | Screening methods and assays for use with transmembrane proteins, in particular with gpcrs |
| US12123814B1 (en) * | 2019-06-14 | 2024-10-22 | Pierce Biotechnology, Inc. | G- protein coupled receptor extraction formulations |
| WO2020257405A1 (en) * | 2019-06-18 | 2020-12-24 | Musc Foundation For Research Development | Compositions and methods targeting the nucleotide free state of ras to block oncogenic signaling and transformation |
| JP2022550497A (ja) | 2019-06-21 | 2022-12-02 | ツイスト バイオサイエンス コーポレーション | バーコードに基づいた核酸配列アセンブリ |
| AU2020355027A1 (en) | 2019-09-23 | 2022-04-21 | Twist Bioscience Corporation | Antibodies that bind CD3 Epsilon |
| CA3155629A1 (en) | 2019-09-23 | 2021-04-01 | Twist Bioscience Corporation | Variant nucleic acid libraries for crth2 |
| FR3106830B1 (fr) | 2020-01-30 | 2022-02-11 | Cisbio Bioassays | Anticorps anti-protéine G alpha |
| US12391762B2 (en) | 2020-08-26 | 2025-08-19 | Twist Bioscience Corporation | Methods and compositions relating to GLP1R variants |
| WO2023154533A2 (en) * | 2022-02-14 | 2023-08-17 | Twist Bioscience Corporation | Combinatorial dna assembly for multispecific antibodies |
| US20250164508A1 (en) * | 2022-02-17 | 2025-05-22 | The Regents Of The University Of Michigan | G-protein coupled receptor assay |
| CN114702570B (zh) * | 2022-03-23 | 2022-12-06 | 山东大学 | aGPCR拮抗剂 |
| WO2024083843A1 (en) | 2022-10-18 | 2024-04-25 | Confo Therapeutics N.V. | Amino acid sequences directed against the melanocortin 4 receptor and polypeptides comprising the same for the treatment of mc4r-related diseases and disorders |
| US11862295B1 (en) | 2022-11-08 | 2024-01-02 | United Arab Emirates University | Method of classifying conformers |
| CN116425888B (zh) * | 2023-04-24 | 2024-11-05 | 徐州医科大学 | 一种多肽tat-v2r1c及其应用 |
| US12176075B1 (en) | 2023-11-14 | 2024-12-24 | United Arab Emirates University | Method of matching conformers of different molecules |
| WO2025129064A1 (en) * | 2023-12-15 | 2025-06-19 | Eve Bio, Llc | Spliced rna barcodes for multiplexed cell-based high throughput screening |
| WO2025149667A1 (en) | 2024-01-12 | 2025-07-17 | Pheon Therapeutics Ltd | Antibody drug conjugates and uses thereof |
| EP4620523A1 (en) * | 2024-03-22 | 2025-09-24 | Genome Research Limited | Mutants of the beta2-adrenergic receptor with altered activity and their use in disease treatment. |
| JP2025167890A (ja) * | 2024-04-26 | 2025-11-07 | 国立大学法人 東京大学 | 標的タンパク質に付加されるためのタンパク質 |
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|---|---|---|---|---|
| EP0656946B2 (en) | 1992-08-21 | 2010-03-31 | Vrije Universiteit Brussel | Immunoglobulins devoid of light chains |
| US5721121A (en) | 1995-06-06 | 1998-02-24 | Genentech, Inc. | Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein |
| WO1999037681A2 (en) | 1998-01-26 | 1999-07-29 | Unilever Plc | Method for producing antibody fragments |
| WO2000043507A1 (en) | 1999-01-19 | 2000-07-27 | Unilever Plc | Method for producing antibody fragments |
| US20030190598A1 (en) | 2000-05-26 | 2003-10-09 | Jasmid Tanha | Single-domain antigen-binding antibody fragments derived from llama antibodies |
| US7294472B2 (en) * | 2001-03-14 | 2007-11-13 | Caden Biosciences | Method for identifying modulators of G protein coupled receptor signaling |
| US7208279B2 (en) | 2001-03-14 | 2007-04-24 | Caden Biosciences, Inc. | Method for identifying inhibitors of G protein coupled receptor signaling |
| GB0110029D0 (en) | 2001-04-24 | 2001-06-13 | Grosveld Frank | Transgenic animal |
| WO2004035614A1 (en) * | 2001-07-11 | 2004-04-29 | Karo Bio Ab | Synthetic or partially purified peptides which can bind to specific subunits of g proteins and uses thereof |
| JP4213586B2 (ja) | 2001-09-13 | 2009-01-21 | 株式会社抗体研究所 | ラクダ抗体ライブラリーの作製方法 |
| JP2005289809A (ja) | 2001-10-24 | 2005-10-20 | Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) | 突然変異重鎖抗体 |
| GB0228210D0 (en) | 2002-12-03 | 2003-01-08 | Babraham Inst | Single chain antibodies |
| KR101025143B1 (ko) | 2002-12-31 | 2011-04-01 | 넥타르 테라퓨틱스 | 가수분해상으로 안정한 말레이미드－종결 중합체 |
| WO2006086883A1 (en) * | 2005-02-16 | 2006-08-24 | Universite De Montreal | Biosensors for monitoring receptor-mediated g-protein activation |
| EP2057191A1 (en) | 2006-08-18 | 2009-05-13 | Ablynx N.V. | Amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of deseases and disorders associated with il-6-mediated signalling |
| WO2009051633A1 (en) * | 2007-10-15 | 2009-04-23 | The Board Of Trustees Of The Leland Stanford Junior University | Gpcr crystalization method using an antibody |
| US9212226B2 (en) * | 2008-05-16 | 2015-12-15 | Ablynx N.V. | Amino acid sequences directed against CXCR4 and other GPCRs and compounds comprising the same |
| WO2009147196A1 (en) | 2008-06-04 | 2009-12-10 | Vib Vzw | Hiv-rev-multimerization inhibiting nanobodies |
| EP2352764B1 (en) * | 2008-10-14 | 2018-03-28 | Ablynx N.V. | AMINO ACID SEQUENCES TARGETING HUMAN CD4 and CXCR4, CCR5, TLR4, ALPHAV INTEGRIN, BETA3-INTEGRIN,BETA1-INTEGRIN, HUMAN ALPHA2-INTEGRIN, CD81, SR-BI, CLAUDIN-1, CLAUDIN-6 AND/OR CLAUDIN-9, RESPECTIVELY, AND NEUTRALIZING VIRAL ENTRY |
| JP2012510806A (ja) | 2008-12-08 | 2012-05-17 | コンプリクス エン ヴェー | 単鎖逆平行コイルドコイルタンパク質 |
| GB201014715D0 (en) | 2010-09-06 | 2010-10-20 | Vib Vzw | Nanobodies stabilizing functional conformational states of GPCRS |
| US8765414B2 (en) | 2011-03-15 | 2014-07-01 | The Board Of Trustees Of The Leland Stanford Junior University | GPCR fusion protein containing an N-terminal autonomously folding stable domain, and crystals of the same |
| ES2662372T3 (es) | 2011-06-21 | 2018-04-06 | Vib Vzw | Dominios de unión dirigidos contra complejos GPCR:proteína G y usos derivados de los mismos |
-
2012
- 2012-06-21 ES ES12730478.0T patent/ES2662372T3/es active Active
- 2012-06-21 NO NO12730478A patent/NO2723764T3/no unknown
- 2012-06-21 CN CN201710728087.8A patent/CN107857810A/zh active Pending
- 2012-06-21 DK DK12730478.0T patent/DK2723764T3/en active
- 2012-06-21 EP EP23193788.9A patent/EP4282882A3/en active Pending
- 2012-06-21 EP EP12730478.0A patent/EP2723764B1/en active Active
- 2012-06-21 EP EP17209368.4A patent/EP3345919A1/en active Pending
- 2012-06-21 PT PT127304780T patent/PT2723764T/pt unknown
- 2012-06-21 CA CA2839834A patent/CA2839834A1/en active Pending
- 2012-06-21 US US14/129,100 patent/US9695227B2/en active Active
- 2012-06-21 WO PCT/EP2012/062036 patent/WO2012175643A2/en not_active Ceased
- 2012-06-21 HU HUE12730478A patent/HUE036982T2/hu unknown
- 2012-06-21 CN CN201280040585.9A patent/CN104053667B/zh active Active
- 2012-06-21 JP JP2014516358A patent/JP6164535B2/ja active Active
- 2012-06-21 PL PL12730478T patent/PL2723764T3/pl unknown
- 2012-06-21 HR HRP20180443TT patent/HRP20180443T1/hr unknown
- 2012-06-21 AU AU2012273987A patent/AU2012273987B2/en active Active
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2017
- 2017-02-16 JP JP2017026632A patent/JP2017127306A/ja active Pending
- 2017-05-01 US US15/583,540 patent/US10626154B2/en active Active
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2020
- 2020-04-17 US US16/852,235 patent/US12252521B2/en active Active
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2024
- 2024-11-26 US US18/960,784 patent/US20250171510A1/en active Pending