JP2014504865A - 標的分子との結合親和性を有する核酸分子およびその生成方法 - Google Patents

標的分子との結合親和性を有する核酸分子およびその生成方法 Download PDF

Info

Publication number
JP2014504865A
JP2014504865A JP2013547842A JP2013547842A JP2014504865A JP 2014504865 A JP2014504865 A JP 2014504865A JP 2013547842 A JP2013547842 A JP 2013547842A JP 2013547842 A JP2013547842 A JP 2013547842A JP 2014504865 A JP2014504865 A JP 2014504865A
Authority
JP
Japan
Prior art keywords
nucleic acid
acid molecule
reference nucleic
nucleotide
level
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013547842A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014504865A5 (enExample
Inventor
ジャロシュク，フロリアン
クラスマン，スヴェン
セル，シモーネ
パーシェク，ウェルナー
マーシュク，クリスティアン
ヴァター，アクセル
ホフリグ，カイ
Original Assignee
ノクソン ファーマ エージー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2011/002068 external-priority patent/WO2011131371A1/en
Application filed by ノクソン ファーマ エージー filed Critical ノクソン ファーマ エージー
Publication of JP2014504865A publication Critical patent/JP2014504865A/ja
Publication of JP2014504865A5 publication Critical patent/JP2014504865A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2013547842A 2011-01-10 2012-01-10 標的分子との結合親和性を有する核酸分子およびその生成方法 Pending JP2014504865A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP11000117.9 2011-01-10
EP11000117 2011-01-10
EPPCT/EP2011/002068 2011-04-21
PCT/EP2011/002068 WO2011131371A1 (en) 2010-04-21 2011-04-21 Lipid binding nucleic acids
EP11008467.0 2011-10-21
EP11008467 2011-10-21
PCT/EP2012/000089 WO2012095303A1 (en) 2011-01-10 2012-01-10 Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017075557A Division JP2017148055A (ja) 2011-01-10 2017-04-05 標的分子との結合親和性を有する核酸分子およびその生成方法

Publications (2)

Publication Number Publication Date
JP2014504865A true JP2014504865A (ja) 2014-02-27
JP2014504865A5 JP2014504865A5 (enExample) 2015-03-05

Family

ID=45464586

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013547842A Pending JP2014504865A (ja) 2011-01-10 2012-01-10 標的分子との結合親和性を有する核酸分子およびその生成方法
JP2017075557A Pending JP2017148055A (ja) 2011-01-10 2017-04-05 標的分子との結合親和性を有する核酸分子およびその生成方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2017075557A Pending JP2017148055A (ja) 2011-01-10 2017-04-05 標的分子との結合親和性を有する核酸分子およびその生成方法

Country Status (8)

Country Link
US (2) US9976145B2 (enExample)
EP (1) EP2663640B1 (enExample)
JP (2) JP2014504865A (enExample)
KR (2) KR20140026357A (enExample)
CN (1) CN103339258A (enExample)
AU (1) AU2012206750A1 (enExample)
CA (1) CA2824073A1 (enExample)
WO (1) WO2012095303A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012325233A1 (en) * 2011-10-21 2014-04-24 Noxxon Pharma Ag Glucagon binding nucleic acids
MX2014008456A (es) * 2012-01-10 2014-11-25 Noxxon Pharma Ag Acidos nucleicos que enlazan especificamente a cgrp.
PL2802660T3 (pl) * 2012-01-10 2020-07-27 Aptarion Biotech Ag Nowe kwasy nukleinowe wiążące c5a
US11172873B2 (en) 2018-05-17 2021-11-16 The Procter & Gamble Company Systems and methods for hair analysis
MX2020012225A (es) 2018-05-17 2021-01-29 Procter & Gamble Sistemas y metodos para el analisis de la cobertura del cabello.
US11384357B2 (en) 2018-06-29 2022-07-12 The Procter And Gamble Company Aptamers for personal care applications
WO2020214784A1 (en) 2019-04-16 2020-10-22 The Procter & Gamble Company Aptamers for odor control applications
US12039732B2 (en) 2021-04-14 2024-07-16 The Procter & Gamble Company Digital imaging and learning systems and methods for analyzing pixel data of a scalp region of a users scalp to generate one or more user-specific scalp classifications
EP4306640A1 (en) 2022-06-21 2024-01-17 TME Pharma AG Method for treating a tumor in a subject
CA3257623A1 (en) 2022-06-21 2023-12-28 TME Pharma AG METHODS OF TREATING A TUMOR IN A SUBJECT

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6011020A (en) 1990-06-11 2000-01-04 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand complexes
EP0533838B1 (en) 1990-06-11 1997-12-03 NeXstar Pharmaceuticals, Inc. Nucleic acid ligands
DE59708838D1 (de) 1996-08-30 2003-01-09 Jens Peter Fuerste Spiegelselektion und spiegelevolution von nucleinsäuren
US6395029B1 (en) 1999-01-19 2002-05-28 The Children's Hospital Of Philadelphia Sustained delivery of polyionic bioactive agents
US6652886B2 (en) 2001-02-16 2003-11-25 Expression Genetics Biodegradable cationic copolymers of poly (alkylenimine) and poly (ethylene glycol) for the delivery of bioactive agents
ATE452655T1 (de) 2001-06-10 2010-01-15 Noxxon Pharma Ag Verwendung von l-polynukleotiden zur diagnostischen bilderzeugung
ATE469164T1 (de) 2001-10-26 2010-06-15 Noxxon Pharma Ag Modifizierte l-nukleinsäure
EP1306382A1 (de) 2001-10-26 2003-05-02 Noxxon Pharma AG Modifizierte L-Nukleinsäure
ATE441706T1 (de) 2002-02-20 2009-09-15 Gen Hospital Corp Konjugate mit biologisch abbaubarem polymer und verwendung dafür
RU2394041C2 (ru) 2003-04-13 2010-07-10 Энзон Фармасьютикалз, Инк. Полимерные олигонуклеотидные пролекарства
GB0314472D0 (en) 2003-06-20 2003-07-23 Warwick Effect Polymers Ltd Polymer
CN1894407A (zh) * 2003-11-10 2007-01-10 诺松制药股份公司 特异性结合生物活性生长素释放肽的核酸
EP1713509A2 (de) 2004-02-09 2006-10-25 Noxxon Pharma AG Verfahren zur herstellung von konjugaten aus polysacchariden und polynukleotiden
CA2940803A1 (en) 2004-03-23 2005-10-27 Ascendis Pharma Gmbh Prodrug linker
US7579450B2 (en) 2004-04-26 2009-08-25 Archemix Corp. Nucleic acid ligands specific to immunoglobulin E and their use as atopic disease therapeutics
WO2006052790A2 (en) 2004-11-05 2006-05-18 The Children's Hospital Of Philadelphia Biodegradable linkers for molecular therapies
US20090075342A1 (en) 2005-04-26 2009-03-19 Sharon Cload Metabolic profile directed aptamer medicinal chemistry
US20120101267A1 (en) 2005-10-07 2012-04-26 Alison Velyian Todd Multicomponent nucleic acid enzymes with cleavage, ligase or other activity and methods for their use
ATE516369T1 (de) 2005-10-07 2011-07-15 Johnson & Johnson Res Pty Ltd Nukleinsäure-enzyme mit mehreren bestandteilen und verfahren zu deren verwendung
AU2007296054B2 (en) 2006-09-15 2013-03-28 Enzon Pharmaceuticals, Inc. Hindered ester-based biodegradable linkers for oligonucleotide delivery
KR101583834B1 (ko) 2007-04-05 2016-01-19 존슨 앤드 존슨 리서치 피티와이 리미티드 핵산 효소 및 복합체 및 이의 사용 방법
CA2732142A1 (en) * 2008-07-28 2010-02-04 Idera Pharmaceuticals, Inc. Modulation of toll-like receptor 9 expression by antisense oligonucleotides
EP2561079A1 (en) 2010-04-21 2013-02-27 Noxxon Pharma AG Lipid binding nucleic acids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JPN5014003217; ＥＬＯＹ　ＢＥＲＮＡＬ-ＭＥＮＤＥＺ: 'ＣＯＮＦＯＲＭＡＴＩＯＮＡＬ　ＤＩＶＥＲＳＩＴＹ　ＶＥＲＳＵＳ　ＮＵＣＬＥＩＣ　ＡＣＩＤ　ＴＲＩＰＬＥＸ　ＳＴＡＢＩＬＩＴＹ，　Ａ　ＣＯＭＢＩＮＡＴＯＲＩＡＬ　ＳＴＵＤＹ' ＪＯＵＲＮＡＬ　ＯＦ　ＢＩＯＬＯＧＩＣＡＬ　ＣＨＥＭＩＳＴＲＹ Ｖ２７６　Ｎ３８, 20010914, Ｐ３５３２０-３５３２７ *
JPN5014003218; ＥＡＴＯＮ　ＢＲＵＣＥ　Ｅ: 'ＰＯＳＴ　ＳＥＬＥＸ　ＣＯＭＢＩＮＡＴＯＲＩＡＬ　ＯＰＴＩＭＩＺＡＴＩＯＮ　ＯＦ　ＡＰＴＡＭＥＲＳ' ＢＩＯＯＲＧＡＮＩＣ　＆　ＭＥＤＩＣＩＮＡＬ　ＣＨＥＭＩＳＴＲＹ Ｖ５　Ｎ６, 19970101, Ｐ１０８７-１０９６, ＰＥＲＧＡＭＯＮ *
JPN5014003221; ＨＯＨＥＩＳＥＬ　ＪＯＥＲＧ　Ｄ: 'ＳＥＱＵＥＮＣＥ-ＩＮＤＥＰＥＮＤＥＮＴ　ＡＮＤ　ＬＩＮＥＡＲ　ＶＡＲＩＡＴＩＯＮ　ＯＦ　ＯＬＩＧＯＮＵＣＬＥＯＴＩＤＥ　ＤＮＡ　ＢＩＮＤＩＮＧ　ＳＴＡＢＩＬＩＴＩＥＳ' ＮＵＣＬＥＩＣ　ＡＣＩＤＳ　ＲＥＳＥＡＲＣＨ Ｖ２４　Ｎ３, 19960201, Ｐ４３０-４３２, ＯＸＦＯＲＤ　ＵＮＩＶＥＲＳＩＴＹ　ＰＲＥＳＳ *
JPN6016047098; ＲＮＡ， ２０００， Ｖｏｌ． ６， ｐｐ． ６５９-６６７ *

Also Published As

Publication number Publication date
KR102080142B1 (ko) 2020-02-21
AU2012206750A1 (en) 2013-07-18
WO2012095303A1 (en) 2012-07-19
CA2824073A1 (en) 2012-07-19
EP2663640B1 (en) 2020-11-04
KR20190067260A (ko) 2019-06-14
EP2663640A1 (en) 2013-11-20
US20140350088A1 (en) 2014-11-27
US9976145B2 (en) 2018-05-22
JP2017148055A (ja) 2017-08-31
KR20140026357A (ko) 2014-03-05
US20190367921A1 (en) 2019-12-05
CN103339258A (zh) 2013-10-02

Similar Documents

Publication Publication Date Title
JP2017148055A (ja) 標的分子との結合親和性を有する核酸分子およびその生成方法
US7589073B2 (en) Aptamers to von Willebrand Factor and their use as thrombotic disease therapeutics
CN103946381B (zh) Ngf适体及其应用
US7998940B2 (en) Aptamers to von Willebrand factor and their use as thrombotic disease therapeutics
US7566701B2 (en) Aptamers to von Willebrand Factor and their use as thrombotic disease therapeutics
KR101466931B1 (ko) Sdf-1 결합형 b형 핵산분자
WO2005111238A2 (en) Aptamer-mediated intracellular delivery of therapeutic oligonucleotides
CA2814076A1 (en) Nucleic acid modulators of clec-2
US9163243B2 (en) Nucleic acids specifically binding CGRP
JP2014533098A (ja) グルカゴン結合核酸
WO2007058323A1 (ja) 核酸ホモポリマー結合機能性核酸医薬品の製造法
WO2023176863A1 (ja) ＲＮＡｉ活性を有する化学修飾オリゴヌクレオチド
AU2012244176B2 (en) Modulators of pharmacological agents
CN113383079A (zh) Cxcl8结合性核酸
HK1146296A (en) C5a binding nucleic acids

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150109

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150109

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160426

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160729

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20161206