JP2014111577A - トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 - Google Patents
トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 Download PDFInfo
- Publication number
- JP2014111577A JP2014111577A JP2013224888A JP2013224888A JP2014111577A JP 2014111577 A JP2014111577 A JP 2014111577A JP 2013224888 A JP2013224888 A JP 2013224888A JP 2013224888 A JP2013224888 A JP 2013224888A JP 2014111577 A JP2014111577 A JP 2014111577A
- Authority
- JP
- Japan
- Prior art keywords
- yeast
- colon cancer
- glucosylceramide
- extraction
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000002305 glucosylceramides Chemical class 0.000 title claims abstract description 33
- 206010009944 Colon cancer Diseases 0.000 title claims abstract description 28
- 208000029742 colonic neoplasm Diseases 0.000 title claims abstract description 23
- 239000002246 antineoplastic agent Substances 0.000 title claims abstract description 10
- 241000006364 Torula Species 0.000 title claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract description 39
- 238000000605 extraction Methods 0.000 claims abstract description 26
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 14
- 239000012138 yeast extract Substances 0.000 claims description 14
- 210000005253 yeast cell Anatomy 0.000 claims description 9
- 235000013361 beverage Nutrition 0.000 claims description 6
- 239000000284 extract Substances 0.000 abstract description 16
- 239000000203 mixture Substances 0.000 abstract description 16
- 230000002401 inhibitory effect Effects 0.000 abstract description 8
- 206010028980 Neoplasm Diseases 0.000 abstract description 5
- 201000011510 cancer Diseases 0.000 abstract description 3
- 239000002440 industrial waste Substances 0.000 abstract description 3
- 230000035755 proliferation Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 16
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 14
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 14
- 229940106189 ceramide Drugs 0.000 description 14
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 14
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- DGAKHGXRMXWHBX-ONEGZZNKSA-N Azoxymethane Chemical compound C\N=[N+](/C)[O-] DGAKHGXRMXWHBX-ONEGZZNKSA-N 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 208000005623 Carcinogenesis Diseases 0.000 description 5
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000036952 cancer formation Effects 0.000 description 5
- 231100000504 carcinogenesis Toxicity 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 238000000105 evaporative light scattering detection Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- -1 manganese, iron ions Chemical class 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 2
- 206010057248 Cell death Diseases 0.000 description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 description 2
- 208000004232 Enteritis Diseases 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 208000015634 Rectal Neoplasms Diseases 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 229930182558 Sterol Natural products 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 229910002091 carbon monoxide Inorganic materials 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 230000009422 growth inhibiting effect Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- YLLIGHVCTUPGEH-UHFFFAOYSA-M potassium;ethanol;hydroxide Chemical compound [OH-].[K+].CCO YLLIGHVCTUPGEH-UHFFFAOYSA-M 0.000 description 2
- 206010038038 rectal cancer Diseases 0.000 description 2
- 201000001275 rectum cancer Diseases 0.000 description 2
- 230000028043 self proteolysis Effects 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- CTIHYOZNWNAKHD-UHFFFAOYSA-N 4-methoxybenzaldehyde;sulfuric acid Chemical compound OS(O)(=O)=O.COC1=CC=C(C=O)C=C1 CTIHYOZNWNAKHD-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000010609 cell counting kit-8 assay Methods 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 125000001549 ceramide group Chemical group 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- IJKVHSBPTUYDLN-UHFFFAOYSA-N dihydroxy(oxo)silane Chemical compound O[Si](O)=O IJKVHSBPTUYDLN-UHFFFAOYSA-N 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012156 elution solvent Substances 0.000 description 1
- 235000015897 energy drink Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 125000001925 glucosylceramide group Chemical group 0.000 description 1
- 150000002339 glycosphingolipids Chemical class 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002700 inhibitory effect on cancer Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 235000011147 magnesium chloride Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000020124 milk-based beverage Nutrition 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- 239000006012 monoammonium phosphate Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 125000005342 perphosphate group Chemical group 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000011118 potassium hydroxide Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000011962 puddings Nutrition 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 235000012046 side dish Nutrition 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 150000003410 sphingosines Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 230000005740 tumor formation Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
【解決手段】酵母エキス抽出後の酵母菌体から抽出したグルコシルセラミド組成物をモデルマウス及びヒト大腸癌細胞HT−29を用いて癌細胞の増殖抑制作用を評価したところ、酵母由来のグルコシルセラミドは、強い大腸癌抑制効果があることを見出した。
【選択図】図1
Description
(1)、酵母エキス抽出後のトルラ酵母菌体から抽出されたグルコシルセラミドを含有する大腸癌抑制剤、
(2)、(1)の大腸癌抑制剤を含有する飲食品。
前処理としてグルコシルセラミド組成物20mgを2mlの0.4N KOH EtOHに溶解し、37℃で2時間インキュベートした。インキュベート後は0.4N HCl EtOHを2ml加えて中和し、遠心分離後の上清4μlをTLCに供した。TLCはシリカゲルプレート(メルク社製Silicagel60、層厚0.25m)を使用し、クロロホルム:エタノール:水=65:16:2(容量比)で展開した。展開後はシリカゲルプレートを乾燥させ、アニスアルデヒド硫酸試液を噴霧して加熱することで発色させた。
前処理としてグルコシルセラミド組成物20mgを2mlの0.4N KOH EtOHに溶解し、37℃で2時間インキュベートした。インキュベート後は0.4N HCl EtOHを2ml加えて中和し、塩類をフィルター除去してからHPLCに供した。カラムにはGLサイエンス社製Inertsil 100Aを用い、グルコシルセラミドの検出は蒸発光散乱検出器(島津製ELSD−LTII)で行った。溶出溶媒にはクロロホルムとメタノール:水=95:5(容量比)のグラジエントを用いた。カラム温度は35℃、流速は1ml/min、ドリフトチューブ温度は40℃で窒素ガス圧力は350kPaであった。
キャンディダ・ウチリスCS7529株(FERMP−3340)を予めYPD培地(酵母エキス1%、ポリペプトン2%、グルコース2%)を含む三角フラスコで種母培養し、これを30L容発酵槽に18L培地に1〜2%植菌した。培地組成は、グルコース4%、燐酸一アンモニウム0.3%、硫酸アンモニウム0.161%、塩化カリウム0.137%、硫酸マグネシウム0.08%、硫酸銅1.6ppm、硫酸鉄14ppm、硫酸マンガン16ppm、硫酸亜鉛14ppmを用いた。培養条件は、pH4.0、培養温度30℃、通気量1vvm、撹拌600rpmで行い、アンモニアを添加しpHのコントロールを行った。16時間菌体培養した後、培養液を回収し、遠心分離により集菌し、180gの湿潤酵母菌体を得た。
菌体培養後の湿潤酵母菌体を蒸留水に懸濁して遠心分離を繰り返すことで洗浄した。洗浄後は湿潤菌体を蒸留水に再度懸濁するか、凍結乾燥または熱風乾燥させた乾燥菌体を蒸留水に懸濁し、以下の条件に調整することでエキス抽出を行った。
自己消化: 1N HClでpH5.0に調整後、55℃で4時間攪拌
酵素抽出:1N NaOHでpH7に調整後、細胞壁溶解酵素(ツニカーゼ)或いはプロテアーゼで55℃、4時間攪拌抽出(再現性確認中)
酸抽出:1N硫酸でpH2以下に調整後、65℃で2分間攪拌抽出
アルカリ抽出:2N NaOHでpH13に調整後、70℃で20分間攪拌抽出
菌体培養後に洗浄し、熱風乾燥させたトルラ酵母の乾燥菌体5kgを蒸留水50Lに懸濁し、2N NaOHでpH13.0に調整した後、70℃で30分間エキス抽出した。抽出後は遠心分離で酵母残渣を回収し、酵母残渣の蒸留水への懸濁と遠心分離を3回繰り返すことで洗浄した。洗浄後は酵母残渣を真空乾燥することで3.3kgのエキス抽出酵母残渣が得られた。得られた酵母残渣全量を2倍量の90%エタノールに懸濁し、60℃で10時間攪拌してグルコシルセラミドを抽出した。遠心分離により抽出液を回収し、酵母残渣をエタノールで3回洗浄した洗浄液と合わせて濃縮した結果、抽出物300gが得られた。これをグルコシルセラミド含有組成物とし、HPLC−ELSDで分析した結果、グルコシルセラミドが2.1%含有されていた。またTLCによる分析の結果、夾雑物のステロール配糖体は確認されなかった。大腸癌抑制効果の確認用のサンプルには、上記抽出物15gをさらにシリカカラムで精製することで、グルコシルセラミド含量を34%含有する組成物11gが得られた。得られた酵母由来グルコシルセラミド組精製物を用いで大腸癌抑制作用の評価を行った。
製造例1で得た酵母セラミドについて、癌細胞に対する増殖抑制作用を評価した。
酵母セラミドを99.7%エタノールに溶解し、各濃度に調整して被検物質とした。
発癌物質であるAOM(アゾキシメタン)を腹腔内投与後、腸炎誘発剤DSS(デキストラン硫酸ナトリウム)で腸炎を起こさせ、発癌を促進させるモデル(Rosenberg, D.W. et al.. Carcinogenesis, (2009) 30 (2): 183-196)により、製造例1で得た酵母セラミドのマウスでの効果を確認した。
Claims (2)
- 酵母エキス抽出後のトルラ酵母菌体から抽出されたグルコシルセラミドを含有する大腸癌抑制剤。
- 請求項1の大腸癌抑制剤を含有する飲食品。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2013224888A JP6261031B2 (ja) | 2012-10-30 | 2013-10-30 | トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012238744 | 2012-10-30 | ||
JP2012238744 | 2012-10-30 | ||
JP2013224888A JP6261031B2 (ja) | 2012-10-30 | 2013-10-30 | トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2014111577A true JP2014111577A (ja) | 2014-06-19 |
JP6261031B2 JP6261031B2 (ja) | 2018-01-17 |
Family
ID=51169011
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013224888A Active JP6261031B2 (ja) | 2012-10-30 | 2013-10-30 | トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP6261031B2 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014087278A (ja) * | 2012-10-30 | 2014-05-15 | Kohjin Life Sciences Co Ltd | トルラ酵母由来グルコシルセラミドの線維芽細胞増殖促進剤としての利用 |
US20170224790A1 (en) * | 2014-10-01 | 2017-08-10 | KOHJIN Life Sciences Co., Ltd. | Fungal glucosylceramide as a vaccine for fungal infections |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4821482B1 (ja) * | 1963-02-08 | 1973-06-29 | ||
JP2001253829A (ja) * | 2000-03-10 | 2001-09-18 | Yakult Honsha Co Ltd | 2次胆汁酸産生抑制剤及び飲食品 |
JP2005501837A (ja) * | 2001-08-01 | 2005-01-20 | ネオーズ テクノロジーズ, インコーポレイテッド | 中性スフィンゴ糖脂質およびグリコシルスフィンゴシンおよびそれらを単離する方法 |
JP2005080583A (ja) * | 2003-09-09 | 2005-03-31 | Kohjin Co Ltd | 吸油抑制剤及び油ちょう食品 |
JP2005187392A (ja) * | 2003-12-25 | 2005-07-14 | Nippon Flour Mills Co Ltd | 大腸ガン抑制剤及びそれを含む食品 |
JP2006328041A (ja) * | 2005-04-25 | 2006-12-07 | Nippon Flour Mills Co Ltd | 抗腫瘍剤、腫瘍細胞増殖抑制剤、免疫賦活剤及び食品 |
JP2012171901A (ja) * | 2011-02-21 | 2012-09-10 | Fiburo Seiyaku Kk | 男性用の発毛および育毛用経口摂取食品 |
JP2014088340A (ja) * | 2012-10-30 | 2014-05-15 | Kohjin Life Sciences Co Ltd | トルラ酵母由来グルコシルセラミドの美白剤としての利用 |
JP6172700B2 (ja) * | 2012-10-30 | 2017-08-02 | 興人ライフサイエンス株式会社 | トルラ酵母由来グルコシルセラミドの線維芽細胞増殖促進剤としての利用 |
-
2013
- 2013-10-30 JP JP2013224888A patent/JP6261031B2/ja active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4821482B1 (ja) * | 1963-02-08 | 1973-06-29 | ||
JP2001253829A (ja) * | 2000-03-10 | 2001-09-18 | Yakult Honsha Co Ltd | 2次胆汁酸産生抑制剤及び飲食品 |
JP2005501837A (ja) * | 2001-08-01 | 2005-01-20 | ネオーズ テクノロジーズ, インコーポレイテッド | 中性スフィンゴ糖脂質およびグリコシルスフィンゴシンおよびそれらを単離する方法 |
JP2005080583A (ja) * | 2003-09-09 | 2005-03-31 | Kohjin Co Ltd | 吸油抑制剤及び油ちょう食品 |
JP2005187392A (ja) * | 2003-12-25 | 2005-07-14 | Nippon Flour Mills Co Ltd | 大腸ガン抑制剤及びそれを含む食品 |
JP2006328041A (ja) * | 2005-04-25 | 2006-12-07 | Nippon Flour Mills Co Ltd | 抗腫瘍剤、腫瘍細胞増殖抑制剤、免疫賦活剤及び食品 |
JP2012171901A (ja) * | 2011-02-21 | 2012-09-10 | Fiburo Seiyaku Kk | 男性用の発毛および育毛用経口摂取食品 |
JP2014088340A (ja) * | 2012-10-30 | 2014-05-15 | Kohjin Life Sciences Co Ltd | トルラ酵母由来グルコシルセラミドの美白剤としての利用 |
JP6172700B2 (ja) * | 2012-10-30 | 2017-08-02 | 興人ライフサイエンス株式会社 | トルラ酵母由来グルコシルセラミドの線維芽細胞増殖促進剤としての利用 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014087278A (ja) * | 2012-10-30 | 2014-05-15 | Kohjin Life Sciences Co Ltd | トルラ酵母由来グルコシルセラミドの線維芽細胞増殖促進剤としての利用 |
US20170224790A1 (en) * | 2014-10-01 | 2017-08-10 | KOHJIN Life Sciences Co., Ltd. | Fungal glucosylceramide as a vaccine for fungal infections |
Also Published As
Publication number | Publication date |
---|---|
JP6261031B2 (ja) | 2018-01-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
TWI558722B (zh) | 酵母萃取物萃取殘渣之利用方法 | |
EP2258831A1 (en) | Yeast mutant and yeast extract | |
JP6172700B2 (ja) | トルラ酵母由来グルコシルセラミドの線維芽細胞増殖促進剤としての利用 | |
JP6281890B2 (ja) | トルラ酵母由来グルコシルセラミドの美白剤としての利用 | |
EP3714701B1 (en) | Composition for type iv allergy | |
JP6261031B2 (ja) | トルラ酵母由来グルコシルセラミドの大腸癌抑制剤としての利用 | |
JP2009207464A (ja) | サッカロマイセス・セレビシエ変異株、及び該変異株を用いたrna高含有酵母の製造方法。 | |
KR20080036612A (ko) | 미생물에 의한 n-아세틸-d-글루코사민의 발효 생산방법 | |
JP6629512B2 (ja) | トルラ酵母由来グルコシルセラミド含有コラーゲン産生促進組成物 | |
JP4068103B2 (ja) | 雲南sl−001菌の培養によるビタミンkの生産方法 | |
CA3085995C (en) | Composition for type i allergy | |
TWI816735B (zh) | 酵母甘露聚糖之用途 | |
JP4620404B2 (ja) | 酵母変異株、グルタチオン高含有酵母の製造方法、その培養物、その分画物、酵母エキスおよびグルタチオン含有飲食品 | |
TWI670016B (zh) | 酵母萃取物之用途 | |
JP5667365B2 (ja) | サッカロマイセス・セレビシエ変異株、及び該変異株を用いた含硫化合物高含有酵母の製造方法。 | |
JP2022110750A (ja) | コラーゲン産生促進組成物 | |
AU2014256403B2 (en) | Yeast mutant and yeast extract | |
WO2022186355A1 (ja) | 6,3'-ジヒドロキシエクオールの産生のための組成物 | |
JPH0889262A (ja) | イノシトールの製造方法および抗生物質耐性株の取得法 | |
JP2018093742A (ja) | オロット酸カリウム塩含有組成物及びその製造方法 | |
JP2011037822A (ja) | トゲドコロ根茎の酵素処理物及び麹菌発酵処理物 | |
JP6552808B2 (ja) | フラボノイド配糖体の製造方法、飲食品の製造方法、及びフラボノイドの配糖体に飲食品適性を付与する方法 | |
Alves | Fracionamento de levedura residual cervejeira para produção de biomoléculas de alto valor agregado | |
ALVES | FRACTIONATION OF SPENT BREWER'S YEAST FOR HIGH VALUE-ADDED BIOMOLECULES PRODUCTION. | |
KR20170035004A (ko) | 알긴산 올리고당의 제조방법 및 상기 제조방법에 의해 제조된 알긴산 올리고당을 포함하는 비만치료 또는 예방용 조성물 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20161005 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20170725 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170823 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20171019 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20171205 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20171207 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6261031 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |