JP2013539370A5 - - Google Patents

Download PDF

Info

Publication number
JP2013539370A5
JP2013539370A5 JP2013528269A JP2013528269A JP2013539370A5 JP 2013539370 A5 JP2013539370 A5 JP 2013539370A5 JP 2013528269 A JP2013528269 A JP 2013528269A JP 2013528269 A JP2013528269 A JP 2013528269A JP 2013539370 A5 JP2013539370 A5 JP 2013539370A5
Authority
JP
Japan
Prior art keywords
host cell
recombinant host
conversion
product
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2013528269A
Other languages
English (en)
Japanese (ja)
Other versions
JP6008858B2 (ja
JP2013539370A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2011/050689 external-priority patent/WO2012033832A2/en
Publication of JP2013539370A publication Critical patent/JP2013539370A/ja
Publication of JP2013539370A5 publication Critical patent/JP2013539370A5/ja
Application granted granted Critical
Publication of JP6008858B2 publication Critical patent/JP6008858B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2013528269A 2010-09-07 2011-09-07 ピルベートからアセトラクテートへの変換を触媒するポリペプチドをコードするポリヌクレオチドの組込み Active JP6008858B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38056310P 2010-09-07 2010-09-07
US61/380,563 2010-09-07
US201161466557P 2011-03-23 2011-03-23
US61/466,557 2011-03-23
PCT/US2011/050689 WO2012033832A2 (en) 2010-09-07 2011-09-07 Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016138178A Division JP6266707B2 (ja) 2010-09-07 2016-07-13 ピルベートからアセトラクテートへの変換を触媒するポリペプチドをコードするポリヌクレオチドの組込み

Publications (3)

Publication Number Publication Date
JP2013539370A JP2013539370A (ja) 2013-10-24
JP2013539370A5 true JP2013539370A5 (https=) 2014-10-16
JP6008858B2 JP6008858B2 (ja) 2016-10-19

Family

ID=45811143

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013528269A Active JP6008858B2 (ja) 2010-09-07 2011-09-07 ピルベートからアセトラクテートへの変換を触媒するポリペプチドをコードするポリヌクレオチドの組込み
JP2016138178A Expired - Fee Related JP6266707B2 (ja) 2010-09-07 2016-07-13 ピルベートからアセトラクテートへの変換を触媒するポリペプチドをコードするポリヌクレオチドの組込み

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016138178A Expired - Fee Related JP6266707B2 (ja) 2010-09-07 2016-07-13 ピルベートからアセトラクテートへの変換を触媒するポリペプチドをコードするポリヌクレオチドの組込み

Country Status (12)

Country Link
US (4) US9267157B2 (https=)
EP (2) EP3269806B1 (https=)
JP (2) JP6008858B2 (https=)
KR (1) KR20130105649A (https=)
CN (2) CN103080298A (https=)
AU (2) AU2011299303B2 (https=)
BR (1) BR112013005386A2 (https=)
CA (2) CA2810244C (https=)
MX (2) MX356668B (https=)
NZ (2) NZ722791A (https=)
WO (1) WO2012033832A2 (https=)
ZA (1) ZA201301237B (https=)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
EP2134849B1 (en) 2007-04-18 2013-07-10 Butamax (TM) Advanced Biofuels LLC Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US9206447B2 (en) 2008-09-29 2015-12-08 Butamax Advanced Biofuels Llc Recombinant yeast host cells comprising FE-S cluster proteins
EP2483401B1 (en) * 2009-09-29 2017-06-21 Butamax (TM) Advanced Biofuels LLC Improved yeast production host cells
NZ600509A (en) * 2009-12-29 2014-08-29 Butamax Tm Advanced Biofuels Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
NZ600585A (en) 2009-12-29 2014-07-25 Butamax Tm Advanced Biofuels Expression of hexose kinase in recombinant host cells
PH12012501658A1 (en) 2010-02-17 2023-04-28 Butamax Tm Advanced Biofuels Improving activity of fe-s cluster requiring proteins
BR112012032172A2 (pt) 2010-06-18 2015-11-24 Butamax Tm Advanced Biofuels método para produzir um álcool e composição
WO2012033832A2 (en) 2010-09-07 2012-03-15 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
BRPI1004630B1 (pt) * 2010-11-12 2019-01-02 Jose Antonio Fabre fonte energética líquida com iniciação por compressão
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
MX347976B (es) 2011-03-24 2017-05-22 Butamax(Tm) Advanced Biofuels Llc Celulas huesped y metodos para producir isobutanol.
CA2837722A1 (en) 2011-06-17 2012-12-20 Butamax Advanced Biofuels Llc Co-products from biofuel production processes and methods of making
WO2013016717A2 (en) * 2011-07-28 2013-01-31 Butamax(Tm) Advanced Biofuels Llc Keto-isovalerate decarboxylase enzymes and methods of use thereof
CA2862450A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Genetic switches for butanol production
CA2861613A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Fermentative production of alcohols
US9689004B2 (en) 2012-03-23 2017-06-27 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
NZ701099A (en) * 2012-05-11 2017-04-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
BR112015003701A2 (pt) 2012-08-22 2017-12-12 Butamax Advanced Biofuels Llc células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição
BR112015005439A8 (pt) * 2012-09-12 2022-01-11 Butamax Advanced Biofuels Llc Método para recuperar um produto alcoólico de um caldo de fermentação e sistema
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
WO2014052670A1 (en) * 2012-09-26 2014-04-03 Butamax (Tm) Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
US9523104B2 (en) 2013-03-12 2016-12-20 Butamax Advanced Biofuels Llc Processes and systems for the production of alcohols
US9441250B2 (en) 2013-03-14 2016-09-13 Butamax Advanced Biofuels Llc Glycerol 3- phosphate dehydrogenase for butanol production
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
WO2014151645A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Process for maximizing biomass growth and butanol yield by feedback control
WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2015002913A1 (en) 2013-07-03 2015-01-08 Butamax Advanced Biofuels Llc Partial adaptation for butanol production
CN103627698A (zh) * 2013-12-05 2014-03-12 江南大学 乙偶姻高耐受性菌株的选育和用该菌株发酵生产乙偶姻
EP3180440A4 (en) 2014-08-11 2018-01-10 Butamax Advanced Biofuels LLC Yeast preparations and methods of making the same
DK3230459T3 (da) * 2014-12-08 2020-12-07 Lanzatech New Zealand Ltd Rekombinante mikroorganismer med øget strømning gennem en fermenteringsbane
CN109897871A (zh) * 2017-12-07 2019-06-18 中国科学院大连化学物理研究所 一种酶法催化二元醇制备二元胺的方法
CN110468092B (zh) * 2019-08-26 2021-10-01 天津科技大学 一株高产l-缬氨酸的基因工程菌及其构建方法与应用
CN115927136B (zh) * 2022-08-01 2023-06-09 长江师范学院 一种产四甲基吡嗪的重组乳酸菌、其构建方法及其应用
CN116024201B (zh) * 2022-12-23 2024-03-19 天津大学 α-乙酰乳酸脱羧酶突变体及其在生产乙偶姻中的应用
CN116640753B (zh) * 2023-07-20 2023-10-13 中国科学院天津工业生物技术研究所 丙酮酸脱羧酶基因FvPDC6及其在提高威尼斯镰刀菌菌丝蛋白产量中的应用

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US20070031950A1 (en) 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
US6432688B1 (en) 1999-01-18 2002-08-13 Daicel Chemical Industries, Ltd. Amino alcohol dehydrogenase converts keto alcohol to amino alcohol and amino alcohol to keto alcohol
US7405068B2 (en) 2003-05-02 2008-07-29 Tate & Lyle Ingredients Americas, Inc. Pyruvate producing yeast strain
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9297028B2 (en) 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
MX359740B (es) 2005-10-26 2018-10-09 Du Pont Produccion fermentativa de alcoholes de cuatro atomos de carbono.
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
US8273558B2 (en) 2005-10-26 2012-09-25 Butamax(Tm) Advanced Biofuels Llc Fermentive production of four carbon alcohols
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
US8206970B2 (en) 2006-05-02 2012-06-26 Butamax(Tm) Advanced Biofuels Llc Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
US8828704B2 (en) 2006-05-02 2014-09-09 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US7659104B2 (en) 2006-05-05 2010-02-09 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US7541173B2 (en) 2006-06-15 2009-06-02 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US8017364B2 (en) 2006-12-12 2011-09-13 Butamax(Tm) Advanced Biofuels Llc Solvent tolerant microorganisms
EP2134849B1 (en) 2007-04-18 2013-07-10 Butamax (TM) Advanced Biofuels LLC Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8426174B2 (en) * 2007-05-02 2013-04-23 Butamax(Tm) Advanced Biofuels Llc Method for the production of 2-butanol
US20080274522A1 (en) * 2007-05-02 2008-11-06 Bramucci Michael G Method for the production of 2-butanone
ES2553726T3 (es) 2007-12-20 2015-12-11 Butamax (Tm) Advanced Biofuels Llc Cetol-ácido reductoisomerasa que utiliza NADH
US8372612B2 (en) 2007-12-21 2013-02-12 Butamax(Tm) Advanced Biofuels Llc Production of four carbon alcohols using improved strain
US20090162911A1 (en) 2007-12-21 2009-06-25 E.I. Du Pont De Nemours And Company Strain for butanol production
US8518678B2 (en) 2007-12-21 2013-08-27 Butamax(Tm) Advanced Biofuels Llc Strain comprising increased expression of a CFA coding region for butanol production
US8455239B2 (en) 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
DE102008010121B4 (de) * 2008-02-20 2013-11-21 Butalco Gmbh Fermentative Produktion von Isobutanol mit Hefe
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US8389252B2 (en) 2008-05-12 2013-03-05 Butamax(Tm) Advanced Biofuels Llc Yeast strain for production of four carbon alcohols
WO2009143455A2 (en) 2008-05-22 2009-11-26 Microbia, Inc. Engineering resistance to aliphatic alcohols
WO2009149240A1 (en) * 2008-06-04 2009-12-10 E. I. Du Pont De Nemours And Company Deletion mutants for the production of isobutanol
US8828695B2 (en) 2008-06-04 2014-09-09 Butamax Advanced Biofuels Llc Method for producing butanol using two-phase extractive fermentation
AU2009256148B2 (en) 2008-06-04 2014-11-27 Butamax(Tm) Advanced Biofuels Llc A method for producing butanol using two-phase extractive fermentation
BRPI0909989A2 (pt) 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
US9206447B2 (en) 2008-09-29 2015-12-08 Butamax Advanced Biofuels Llc Recombinant yeast host cells comprising FE-S cluster proteins
AU2009296227A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
CA2736420A1 (en) 2008-09-29 2010-04-01 Butamaxtm Advanced Biofuels Llc Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
MX2011003238A (es) 2008-09-29 2011-04-28 Butamax Tm Advanced Biofuels Identificacion y uso de dihidroxiacido deshidratasas [2fe-2s] bacterianas.
WO2010037119A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
US20160222370A1 (en) 2008-09-29 2016-08-04 Butamax Advanced Biofuels Llc Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
CA2737112A1 (en) 2008-10-27 2010-06-03 Butamax Advanced Biofuels Llc Carbon pathway optimized production hosts for the production of isobutanol
US8828694B2 (en) 2008-11-13 2014-09-09 Butamax Advanced Biofuels Llc Production of isobutanol in yeast mitochondria
US8465964B2 (en) 2008-11-13 2013-06-18 Butamax (TM) Advanced Biofules LLC Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
US8652823B2 (en) 2008-12-03 2014-02-18 Butamax(Tm) Advanced Biofuels Llc Strain for butanol production with increased membrane unsaturated trans fatty acids
DE102008062847A1 (de) 2008-12-23 2010-06-24 Jt Optical Engine Gmbh + Co. Kg Spleißverbindung zwischen zwei optischen Fasern sowie Verfahren zum Herstellen einer solchen Spleißverbindung
WO2010075504A2 (en) 2008-12-23 2010-07-01 Gevo, Inc. Engineered microorganisms for the production of one or more target compounds
US8795992B2 (en) 2008-12-29 2014-08-05 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall integrity pathway
US8557562B2 (en) 2008-12-29 2013-10-15 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving filamentous growth response
US8455225B2 (en) 2008-12-29 2013-06-04 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
BRPI1006492A2 (pt) 2009-04-13 2019-09-24 Butamax Advanced Biofuels Llc método para a recuperação de butanol a partir de um meio de fermentação, método para a produção de butanol e mistura de duas fases
US20120058541A1 (en) 2009-05-22 2012-03-08 Butamax(Tm) Advanced Biofuels Llc Engineering resistance to aliphatic alcohols
AU2010300722A1 (en) 2009-09-29 2012-04-12 Butamax(Tm) Advanced Biofuels Llc Improved flux to acetolactate-derived products in lactic acid bacteria
EP2483401B1 (en) 2009-09-29 2017-06-21 Butamax (TM) Advanced Biofuels LLC Improved yeast production host cells
AU2010300642B2 (en) 2009-09-29 2015-05-14 Butamax(Tm) Advanced Biofuels Llc Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes
US20110195505A1 (en) 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
NZ600509A (en) 2009-12-29 2014-08-29 Butamax Tm Advanced Biofuels Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
NZ600585A (en) 2009-12-29 2014-07-25 Butamax Tm Advanced Biofuels Expression of hexose kinase in recombinant host cells
PH12012501658A1 (en) 2010-02-17 2023-04-28 Butamax Tm Advanced Biofuels Improving activity of fe-s cluster requiring proteins
CA2801576A1 (en) 2010-06-17 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Yeast production culture for the production of butanol
BR112012032172A2 (pt) 2010-06-18 2015-11-24 Butamax Tm Advanced Biofuels método para produzir um álcool e composição
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
US8697404B2 (en) 2010-06-18 2014-04-15 Butamax Advanced Biofuels Llc Enzymatic production of alcohol esters for recovery of diols produced by fermentation
WO2012033832A2 (en) 2010-09-07 2012-03-15 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
MX347976B (es) 2011-03-24 2017-05-22 Butamax(Tm) Advanced Biofuels Llc Celulas huesped y metodos para producir isobutanol.
US20120258873A1 (en) 2011-04-06 2012-10-11 Butamax(Tm) Advanced Biofuels Llc Reduction of 2,3-dihydroxy-2-methyl butyrate (dhmb) in butanol production
CA2838519A1 (en) 2011-06-17 2012-12-20 Butamax Advanced Biofuels Llc Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
WO2013016717A2 (en) 2011-07-28 2013-01-31 Butamax(Tm) Advanced Biofuels Llc Keto-isovalerate decarboxylase enzymes and methods of use thereof
CA2862450A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Genetic switches for butanol production
CA2861613A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Fermentative production of alcohols
US9689004B2 (en) 2012-03-23 2017-06-27 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
NZ701099A (en) 2012-05-11 2017-04-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
BR112015003701A2 (pt) 2012-08-22 2017-12-12 Butamax Advanced Biofuels Llc células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
WO2014052670A1 (en) 2012-09-26 2014-04-03 Butamax (Tm) Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US20140186911A1 (en) 2012-12-28 2014-07-03 Butamax Advanced Biofuels Llc Recombinant host cells and methods for producing butanol
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
US9441250B2 (en) 2013-03-14 2016-09-13 Butamax Advanced Biofuels Llc Glycerol 3- phosphate dehydrogenase for butanol production
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2015009601A1 (en) 2013-07-16 2015-01-22 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall proteins
US20160326552A1 (en) 2013-12-31 2016-11-10 Butamax Advanced Biofuels Llc Expression of a hap transcriptional complex subunit
WO2015103002A1 (en) 2013-12-31 2015-07-09 Butamax Advanced Biofuels Llc Isobutanol tolerance in yeast with an altered lipid profile

Similar Documents

Publication Publication Date Title
JP2013539370A5 (https=)
CN104619835B (zh) 酮醇酸还原异构酶及其使用方法
US10184139B2 (en) Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
AU2009246622B2 (en) Improved yeast strain for production of four carbon alcohols
US9689004B2 (en) Acetate supplemention of medium for butanologens
US9422581B2 (en) Host cells and methods for production of isobutanol
US9944954B2 (en) Glycerol 3-phosphate dehydrogenase for butanol production
US20110136193A1 (en) Method for producing butanol using extractive fermentation with osmolyte addition
US20140186911A1 (en) Recombinant host cells and methods for producing butanol
CN105102616A (zh) 具有酮醇酸还原异构酶活性的多肽
CN104321436A (zh) 醇类的发酵生产
US20170283765A1 (en) Partial Adaption for Butanol Production
CA2723877A1 (en) A method for producing butanol using two-phase extractive fermentation
US20140093931A1 (en) Production of fermentation products
WO2014151645A1 (en) Process for maximizing biomass growth and butanol yield by feedback control
US20160326551A1 (en) Isobutanol tolerance in yeast with an altered lipid profile