JP2013535207A5 - - Google Patents

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Publication number
JP2013535207A5
JP2013535207A5 JP2013522234A JP2013522234A JP2013535207A5 JP 2013535207 A5 JP2013535207 A5 JP 2013535207A5 JP 2013522234 A JP2013522234 A JP 2013522234A JP 2013522234 A JP2013522234 A JP 2013522234A JP 2013535207 A5 JP2013535207 A5 JP 2013535207A5
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JP
Japan
Prior art keywords
chromatography
exchange chromatography
sialyltransferase
resin
cation exchange
Prior art date
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Application number
JP2013522234A
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English (en)
Japanese (ja)
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JP2013535207A (ja
JP5984267B2 (ja
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Publication date
Priority claimed from EP20100171626 external-priority patent/EP2415779B1/en
Application filed filed Critical
Publication of JP2013535207A publication Critical patent/JP2013535207A/ja
Publication of JP2013535207A5 publication Critical patent/JP2013535207A5/ja
Application granted granted Critical
Publication of JP5984267B2 publication Critical patent/JP5984267B2/ja
Active legal-status Critical Current
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JP2013522234A 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法 Active JP5984267B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
EP10171626.4 2010-08-02
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (3)

Publication Number Publication Date
JP2013535207A JP2013535207A (ja) 2013-09-12
JP2013535207A5 true JP2013535207A5 (https=) 2014-09-11
JP5984267B2 JP5984267B2 (ja) 2016-09-06

Family

ID=42736163

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013522234A Active JP5984267B2 (ja) 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法

Country Status (9)

Country Link
US (1) US9222081B2 (https=)
EP (2) EP2415779B1 (https=)
JP (1) JP5984267B2 (https=)
CN (1) CN103154017B (https=)
CA (1) CA2807397C (https=)
ES (1) ES2534936T3 (https=)
MX (1) MX2013001447A (https=)
PT (1) PT2415779E (https=)
WO (1) WO2012016984A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
KR20150112978A (ko) * 2013-02-06 2015-10-07 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 제제 내의 응집체 함량을 감소시키기 위한 혼합 다관능 금속 친화성 표면들
EP3301115B1 (en) 2013-05-06 2021-11-10 Sanofi Continuous multistep process for purifying antibodies
BR112017021016A2 (pt) * 2015-03-31 2018-07-03 Yeda Research And Development Co Ltd preparação de proteína, composição farmacêutica, proteína de fusão, polinucleotídeo isolado, estrutura de ácido nucleico, método para tratamento de doença ou condição associada ao excesso de glutamato, e método de purificação de um polipeptídeo
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
CN117887794A (zh) 2016-12-21 2024-04-16 豪夫迈·罗氏有限公司 用于体外糖工程化抗体的方法
JP6931058B2 (ja) * 2016-12-21 2021-09-01 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 抗体のインビトロ糖鎖工学における酵素の再使用
IL272987B (en) 2017-09-04 2022-08-01 89Bio Ltd fgf-21 mutant peptide conjugates and uses thereof
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
DK3613486T3 (da) * 2018-08-24 2021-01-04 Uga Biopharma Gmbh Metode og anlæg til rengøring af epo og/eller et epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
WO1995018217A1 (en) 1993-12-24 1995-07-06 The Institute Of Physical And Chemical Research Novel sugar-chain synthetase and process for producing the same
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
ES2411007T3 (es) 2001-10-10 2013-07-04 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
CN1897812B (zh) * 2003-12-03 2011-08-17 生物种属学股份公司 糖聚乙二醇化的粒细胞集落刺激因子
EP1765993A4 (en) 2004-06-03 2008-08-20 Neose Technologies Inc ST6GALNACL TRUNC POLYPEPTIDES AND NUCLEIC ACIDS
US20100143969A1 (en) 2005-03-24 2010-06-10 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
JP2008538181A (ja) * 2005-03-30 2008-10-16 ネオス テクノロジーズ インコーポレイテッド 昆虫細胞系において増殖させたペプチドを生産するための製造方法
US20090047710A1 (en) 2005-11-09 2009-02-19 Neose Technologies, Inc. ST3Gal-1/ST6GalNAc-1 Chimeras
EP2054521A4 (en) * 2006-10-03 2012-12-19 Novo Nordisk As PROCESS FOR CLEANING POLYPEPTIDE CONJUGATES
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

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