CA2807397C - Method of producing and purifying an active soluble sialyltransferase - Google Patents

Method of producing and purifying an active soluble sialyltransferase Download PDF

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Publication number
CA2807397C
CA2807397C CA2807397A CA2807397A CA2807397C CA 2807397 C CA2807397 C CA 2807397C CA 2807397 A CA2807397 A CA 2807397A CA 2807397 A CA2807397 A CA 2807397A CA 2807397 C CA2807397 C CA 2807397C
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CA
Canada
Prior art keywords
sialyltransferase
polypeptide
chromatography
resin
st6galnaci
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CA2807397A
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English (en)
French (fr)
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CA2807397A1 (en
Inventor
Axel Angermann
Christian Scheckermann
Karsten Schmidt
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Ratiopharm GmbH
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Publication of CA2807397A1 publication Critical patent/CA2807397A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2807397A 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase Active CA2807397C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
EP10171626.4 2010-08-02
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (2)

Publication Number Publication Date
CA2807397A1 CA2807397A1 (en) 2012-02-09
CA2807397C true CA2807397C (en) 2019-03-19

Family

ID=42736163

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2807397A Active CA2807397C (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Country Status (9)

Country Link
US (1) US9222081B2 (https=)
EP (2) EP2415779B1 (https=)
JP (1) JP5984267B2 (https=)
CN (1) CN103154017B (https=)
CA (1) CA2807397C (https=)
ES (1) ES2534936T3 (https=)
MX (1) MX2013001447A (https=)
PT (1) PT2415779E (https=)
WO (1) WO2012016984A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
KR20150112978A (ko) * 2013-02-06 2015-10-07 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 제제 내의 응집체 함량을 감소시키기 위한 혼합 다관능 금속 친화성 표면들
EP3301115B1 (en) 2013-05-06 2021-11-10 Sanofi Continuous multistep process for purifying antibodies
BR112017021016A2 (pt) * 2015-03-31 2018-07-03 Yeda Research And Development Co Ltd preparação de proteína, composição farmacêutica, proteína de fusão, polinucleotídeo isolado, estrutura de ácido nucleico, método para tratamento de doença ou condição associada ao excesso de glutamato, e método de purificação de um polipeptídeo
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
CN117887794A (zh) 2016-12-21 2024-04-16 豪夫迈·罗氏有限公司 用于体外糖工程化抗体的方法
JP6931058B2 (ja) * 2016-12-21 2021-09-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 抗体のインビトロ糖鎖工学における酵素の再使用
IL272987B (en) 2017-09-04 2022-08-01 89Bio Ltd fgf-21 mutant peptide conjugates and uses thereof
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
DK3613486T3 (da) * 2018-08-24 2021-01-04 Uga Biopharma Gmbh Metode og anlæg til rengøring af epo og/eller et epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
WO1995018217A1 (en) 1993-12-24 1995-07-06 The Institute Of Physical And Chemical Research Novel sugar-chain synthetase and process for producing the same
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
ES2411007T3 (es) 2001-10-10 2013-07-04 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
CN1897812B (zh) * 2003-12-03 2011-08-17 生物种属学股份公司 糖聚乙二醇化的粒细胞集落刺激因子
EP1765993A4 (en) 2004-06-03 2008-08-20 Neose Technologies Inc ST6GALNACL TRUNC POLYPEPTIDES AND NUCLEIC ACIDS
US20100143969A1 (en) 2005-03-24 2010-06-10 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
JP2008538181A (ja) * 2005-03-30 2008-10-16 ネオス テクノロジーズ インコーポレイテッド 昆虫細胞系において増殖させたペプチドを生産するための製造方法
US20090047710A1 (en) 2005-11-09 2009-02-19 Neose Technologies, Inc. ST3Gal-1/ST6GalNAc-1 Chimeras
EP2054521A4 (en) * 2006-10-03 2012-12-19 Novo Nordisk As PROCESS FOR CLEANING POLYPEPTIDE CONJUGATES
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

Also Published As

Publication number Publication date
MX2013001447A (es) 2013-11-20
US9222081B2 (en) 2015-12-29
JP2013535207A (ja) 2013-09-12
WO2012016984A1 (en) 2012-02-09
PT2415779E (pt) 2015-05-13
CN103154017B (zh) 2016-02-03
JP5984267B2 (ja) 2016-09-06
US20140057316A1 (en) 2014-02-27
CA2807397A1 (en) 2012-02-09
EP2415779A1 (en) 2012-02-08
EP2601207A1 (en) 2013-06-12
CN103154017A (zh) 2013-06-12
EP2601207B1 (en) 2016-12-28
ES2534936T3 (es) 2015-04-30
EP2415779B1 (en) 2015-01-21

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