JP2013518598A - ランダム化配列を含むプライマー及び特異的プライマーを用いる核酸の等温増幅並びにその使用 - Google Patents

ランダム化配列を含むプライマー及び特異的プライマーを用いる核酸の等温増幅並びにその使用 Download PDF

Info

Publication number
JP2013518598A
JP2013518598A JP2012552146A JP2012552146A JP2013518598A JP 2013518598 A JP2013518598 A JP 2013518598A JP 2012552146 A JP2012552146 A JP 2012552146A JP 2012552146 A JP2012552146 A JP 2012552146A JP 2013518598 A JP2013518598 A JP 2013518598A
Authority
JP
Japan
Prior art keywords
nucleic acid
primer
amplification
dna
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2012552146A
Other languages
English (en)
Japanese (ja)
Inventor
ネルソン，ジョン・リチャード
ギャオ，ウェイ
ツァオ，ミン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of JP2013518598A publication Critical patent/JP2013518598A/ja
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2012552146A 2010-02-09 2011-02-08 ランダム化配列を含むプライマー及び特異的プライマーを用いる核酸の等温増幅並びにその使用 Ceased JP2013518598A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/702,884 US20110195457A1 (en) 2010-02-09 2010-02-09 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
US12/702,884 2010-02-09
PCT/US2011/023996 WO2011142861A2 (en) 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof

Publications (1)

Publication Number Publication Date
JP2013518598A true JP2013518598A (ja) 2013-05-23

Family

ID=44354018

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012552146A Ceased JP2013518598A (ja) 2010-02-09 2011-02-08 ランダム化配列を含むプライマー及び特異的プライマーを用いる核酸の等温増幅並びにその使用

Country Status (9)

Country Link
US (1) US20110195457A1 (enExample)
EP (1) EP2534268A4 (enExample)
JP (1) JP2013518598A (enExample)
CN (1) CN102753707A (enExample)
AU (1) AU2011253427B2 (enExample)
BR (1) BR112012019396A2 (enExample)
CA (1) CA2788821A1 (enExample)
IN (1) IN2012DN06299A (enExample)
WO (1) WO2011142861A2 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016529915A (ja) * 2013-09-16 2016-09-29 ゼネラル・エレクトリック・カンパニイ オリゴカチオン複合プライマー配列を使用する等温増幅
JP2016530878A (ja) * 2013-07-26 2016-10-06 ゼネラル・エレクトリック・カンパニイ 循環核酸の回収及び増幅のための方法及び装置
JP2019500852A (ja) * 2015-11-05 2019-01-17 ゼネラル・エレクトリック・カンパニイ リガーゼ支援核酸環状化および増幅
JP2023071073A (ja) * 2021-11-10 2023-05-22 国立大学法人広島大学 鋳型ｄｎａの製造方法
JP2024012528A (ja) * 2015-08-17 2024-01-30 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 微小液滴ベースの多置換増幅（ｍｄａ）方法及び関連組成物

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012050393A (ja) * 2010-09-02 2012-03-15 Sony Corp 核酸等温増幅方法
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US9353393B2 (en) * 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
EP4071253A1 (en) * 2012-07-26 2022-10-12 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
JP6366896B2 (ja) * 2013-03-07 2018-08-01 国立研究開発法人農業・食品産業技術総合研究機構 Ｄｎａ親和性物質含有組成物、ｄｎａ親和性物質の精製方法、ｄｎａ親和性物質含有組成物の製造方法およびｄｎａ親和性物質を精製するためのキット
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9938568B2 (en) 2013-07-26 2018-04-10 General Electric Company Ligase-assisted nucleic acid circularization and amplification
KR101670232B1 (ko) * 2013-10-28 2016-10-31 한국과학기술연구원 다공성 구조체 및 이의 제조방법
WO2015084802A1 (en) * 2013-12-02 2015-06-11 Regents Of The University Of Minnesota Rna amplification and oligonucleotide library preparation
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
AU2015273480A1 (en) 2014-06-11 2016-12-08 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
US11220705B2 (en) 2015-04-24 2022-01-11 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法
CN110506114B (zh) 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒
CN110195056A (zh) * 2019-05-31 2019-09-03 汪雪 一种双引物及其应用
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004512823A (ja) * 2000-06-28 2004-04-30 モレキュラー　ステージング，インコーポレイテッド 核酸配列の多重プライミング増幅プロセス
JP2006525023A (ja) * 2003-04-29 2006-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸配列の多重開始増幅
US20090136924A1 (en) * 2005-04-08 2009-05-28 Larionov Vladimir L Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20090203085A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions
WO2010005365A1 (en) * 2008-07-09 2010-01-14 General Electric Company Unprocessed rolling circle amplification product

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5949544A (en) * 1997-07-14 1999-09-07 Manning; Christopher J. Methods and apparatus for performing very rapid scan spectrometry using a balanced mirror assembly driven at the resonance frequency
ATE346158T1 (de) * 1998-01-09 2006-12-15 Univ Utah Res Found Verfahren zur in vitro amplifikation zirkulärer dna
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
AU2004209001B2 (en) * 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP2374900B1 (en) * 2003-03-07 2016-07-13 Rubicon Genomics, Inc. Polynucleotides for the amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US7211384B2 (en) * 2003-05-28 2007-05-01 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide targets with initially small sample sizes and compositions for practicing the same
CN1950519A (zh) * 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
WO2006119066A2 (en) * 2005-04-29 2006-11-09 The J. Craig Venter Institute Amplification and cloning of single dna molecules using rolling circle amplification
WO2008118679A1 (en) * 2007-03-23 2008-10-02 Ge Healthcare Bio-Sciences Corp. Multiply-primed amplification of circular nucleic acid sequences
SG182159A1 (en) * 2007-06-13 2012-07-30 Decode Genetics Ehf Genetic variants on chr 15q24 as markers for use in diagnosis, prognosis and treatment of exfoliation syndrome and glaucoma
EP2291533B2 (en) * 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004512823A (ja) * 2000-06-28 2004-04-30 モレキュラー　ステージング，インコーポレイテッド 核酸配列の多重プライミング増幅プロセス
JP2006525023A (ja) * 2003-04-29 2006-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸配列の多重開始増幅
US20090136924A1 (en) * 2005-04-08 2009-05-28 Larionov Vladimir L Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20090203085A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions
WO2010005365A1 (en) * 2008-07-09 2010-01-14 General Electric Company Unprocessed rolling circle amplification product

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016530878A (ja) * 2013-07-26 2016-10-06 ゼネラル・エレクトリック・カンパニイ 循環核酸の回収及び増幅のための方法及び装置
JP2016529915A (ja) * 2013-09-16 2016-09-29 ゼネラル・エレクトリック・カンパニイ オリゴカチオン複合プライマー配列を使用する等温増幅
JP2024012528A (ja) * 2015-08-17 2024-01-30 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 微小液滴ベースの多置換増幅（ｍｄａ）方法及び関連組成物
JP2019500852A (ja) * 2015-11-05 2019-01-17 ゼネラル・エレクトリック・カンパニイ リガーゼ支援核酸環状化および増幅
JP2022002538A (ja) * 2015-11-05 2022-01-11 グローバル・ライフ・サイエンシズ・ソリューションズ・オペレーションズ・ユーケー・リミテッド リガーゼ支援核酸環状化および増幅
JP7782906B2 (ja) 2015-11-05 2025-12-09 グローバル ライフ サイエンシズ ソリューションズ オペレーションズ ユーケー リミテッド リガーゼ支援核酸環状化および増幅
JP2023071073A (ja) * 2021-11-10 2023-05-22 国立大学法人広島大学 鋳型ｄｎａの製造方法
JP7748093B2 (ja) 2021-11-10 2025-10-02 国立大学法人広島大学 鋳型ｄｎａの製造方法

Also Published As

Publication number Publication date
CA2788821A1 (en) 2011-11-17
CN102753707A (zh) 2012-10-24
BR112012019396A2 (pt) 2017-06-06
AU2011253427A1 (en) 2012-08-23
EP2534268A4 (en) 2013-09-04
AU2011253427B2 (en) 2015-06-25
WO2011142861A2 (en) 2011-11-17
US20110195457A1 (en) 2011-08-11
IN2012DN06299A (enExample) 2015-09-25
WO2011142861A3 (en) 2012-02-02
WO2011142861A9 (en) 2012-03-15
EP2534268A2 (en) 2012-12-19

Similar Documents

Publication Publication Date Title
AU2011253427B2 (en) Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
AU2013203624B2 (en) Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US6361940B1 (en) Compositions and methods for enhancing hybridization and priming specificity
CN106715706B (zh) 直接从未纯化的生物样本分析核酸的方法
ES2898088T3 (es) Inmuno-PETE
EP4081652B1 (en) Controlled strand-displacement for paired-end sequencing
EP2038429A2 (en) Methods for fragmentation and labeling of nucleic acids
EP2769007A1 (en) Compositions and methods for directional nucleic acid amplification and sequencing
JP2015526069A (ja) Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット
US10443094B2 (en) Solid phase isothermal amplification
CN104093850A (zh) 用于减少非特异性核酸扩增的方法和试剂盒
US20090023151A1 (en) Method For The Labeling And Detection Of Small Polynucleotides
JP6847364B2 (ja) オリゴカチオン複合プライマー配列を使用する等温増幅
CN106460040A (zh) 在低盐条件下的等温扩增
CN112423885A (zh) 多核苷酸合成方法、试剂盒和系统
JPWO2002062993A1 (ja) 増幅核酸及びその固定化物
JP2019500852A (ja) リガーゼ支援核酸環状化および増幅
JP5357893B2 (ja) 迅速超長鎖ｐｃｒのための単一酵素系
WO2013154304A2 (ko) 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
US20110269194A1 (en) Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
EP1624059A2 (en) Method of producing nucleic acid molecules with reduced secondary structure
HK1219297A1 (zh) 使用发卡寡核甘酸扩增核酸的方法
JP7191115B2 (ja) エンドヌクレアーゼ媒介移動平衡（ＥＭ－ＳＥｑ）による核酸の増幅のための方法
CN115279918A (zh) 用于测序的新型核酸模板结构
EP3798319A1 (en) An improved diagnostic and/or sequencing method and kit

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140128

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150414

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150714

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20151006

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160205

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20160212

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160405

A045 Written measure of dismissal of application [lapsed due to lack of payment]

Free format text: JAPANESE INTERMEDIATE CODE: A045

Effective date: 20160830