AU2011253427B2 - Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers - Google Patents

Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers Download PDF

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Publication number
AU2011253427B2
AU2011253427B2 AU2011253427A AU2011253427A AU2011253427B2 AU 2011253427 B2 AU2011253427 B2 AU 2011253427B2 AU 2011253427 A AU2011253427 A AU 2011253427A AU 2011253427 A AU2011253427 A AU 2011253427A AU 2011253427 B2 AU2011253427 B2 AU 2011253427B2
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Australia
Prior art keywords
nucleic acid
primer
dna
amplification
template
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Ceased
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AU2011253427A
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AU2011253427A1 (en
Inventor
Wei Gao
John Richard Nelson
Ming Zhao
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General Electric Co
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General Electric Co
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Priority to AU2013203624A priority Critical patent/AU2013203624B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2011253427A 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers Ceased AU2011253427B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2013203624A AU2013203624B2 (en) 2010-02-09 2013-04-11 Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/702,884 2010-02-09
US12/702,884 US20110195457A1 (en) 2010-02-09 2010-02-09 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
PCT/US2011/023996 WO2011142861A2 (en) 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2013203624A Division AU2013203624B2 (en) 2010-02-09 2013-04-11 Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers

Publications (2)

Publication Number Publication Date
AU2011253427A1 AU2011253427A1 (en) 2012-08-23
AU2011253427B2 true AU2011253427B2 (en) 2015-06-25

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AU2011253427A Ceased AU2011253427B2 (en) 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers

Country Status (9)

Country Link
US (1) US20110195457A1 (enExample)
EP (1) EP2534268A4 (enExample)
JP (1) JP2013518598A (enExample)
CN (1) CN102753707A (enExample)
AU (1) AU2011253427B2 (enExample)
BR (1) BR112012019396A2 (enExample)
CA (1) CA2788821A1 (enExample)
IN (1) IN2012DN06299A (enExample)
WO (1) WO2011142861A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012050393A (ja) * 2010-09-02 2012-03-15 Sony Corp 核酸等温増幅方法
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US9353393B2 (en) * 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
WO2014018093A1 (en) * 2012-07-26 2014-01-30 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
JP6366896B2 (ja) * 2013-03-07 2018-08-01 国立研究開発法人農業・食品産業技術総合研究機構 Ｄｎａ親和性物質含有組成物、ｄｎａ親和性物質の精製方法、ｄｎａ親和性物質含有組成物の製造方法およびｄｎａ親和性物質を精製するためのキット
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9644232B2 (en) * 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9938568B2 (en) 2013-07-26 2018-04-10 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
KR101670232B1 (ko) * 2013-10-28 2016-10-31 한국과학기술연구원 다공성 구조체 및 이의 제조방법
US10428376B2 (en) * 2013-12-02 2019-10-01 Regents Of The University Of Minnesota RNA amplification and oligonucleotide library preparation
US9587263B2 (en) 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
WO2015189336A1 (en) 2014-06-11 2015-12-17 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
EP3337907A4 (en) * 2015-08-17 2019-01-02 The Regents of The University of California Microdroplet-based multiple displacement amplification (mda) methods and related compositions
JP2019500852A (ja) * 2015-11-05 2019-01-17 ゼネラル・エレクトリック・カンパニイ リガーゼ支援核酸環状化および増幅
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法
WO2018232594A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 Pcr引物对及其应用
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒
CN110195056A (zh) * 2019-05-31 2019-09-03 汪雪 一种双引物及其应用
JP7748093B2 (ja) * 2021-11-10 2025-10-02 国立大学法人広島大学 鋳型ｄｎａの製造方法
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203085A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999002939A1 (en) * 1997-07-14 1999-01-21 Spectral Dimensions, Inc. Methods and devices for very rapid scan interferometry
ATE346158T1 (de) * 1998-01-09 2006-12-15 Univ Utah Res Found Verfahren zur in vitro amplifikation zirkulärer dna
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
CA2513535C (en) * 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
DK2374900T3 (en) * 2003-03-07 2016-10-17 Rubicon Genomics Inc Polynucleotides for amplification and analysis of the total genomic and total transcription libraries generated by a DNA polymerization
WO2004097003A2 (en) * 2003-04-29 2004-11-11 Amersham Biosciences Corp Multiply-primed amplification of nucleic acid sequences
US7211384B2 (en) * 2003-05-28 2007-05-01 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide targets with initially small sample sizes and compositions for practicing the same
AU2005216549A1 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US9139849B2 (en) * 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
DK1885880T3 (da) * 2005-04-29 2010-11-08 Synthetic Genomics Inc Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikation
WO2008118679A1 (en) * 2007-03-23 2008-10-02 Ge Healthcare Bio-Sciences Corp. Multiply-primed amplification of circular nucleic acid sequences
CN101784675B (zh) * 2007-06-13 2014-10-29 解码遗传学私营有限责任公司 用于剥脱性综合征和青光眼的诊断、预后和治疗的标记物的chr 15q24上的遗传变异
US8198028B2 (en) * 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100008939A1 (en) * 2008-07-09 2010-01-14 General Electric Company Unprocessed rolling circle amplification product

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203085A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions

Also Published As

Publication number Publication date
EP2534268A4 (en) 2013-09-04
CA2788821A1 (en) 2011-11-17
WO2011142861A9 (en) 2012-03-15
CN102753707A (zh) 2012-10-24
WO2011142861A3 (en) 2012-02-02
IN2012DN06299A (enExample) 2015-09-25
WO2011142861A2 (en) 2011-11-17
EP2534268A2 (en) 2012-12-19
JP2013518598A (ja) 2013-05-23
AU2011253427A1 (en) 2012-08-23
US20110195457A1 (en) 2011-08-11
BR112012019396A2 (pt) 2017-06-06

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