JP2013511292A5 - - Google Patents
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- Publication number
- JP2013511292A5 JP2013511292A5 JP2012541159A JP2012541159A JP2013511292A5 JP 2013511292 A5 JP2013511292 A5 JP 2013511292A5 JP 2012541159 A JP2012541159 A JP 2012541159A JP 2012541159 A JP2012541159 A JP 2012541159A JP 2013511292 A5 JP2013511292 A5 JP 2013511292A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- oligonucleotide probe
- nucleic acid
- probe
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 73
- 239000002751 oligonucleotide probe Substances 0.000 claims 73
- 150000007523 nucleic acids Chemical class 0.000 claims 24
- 108020004707 nucleic acids Proteins 0.000 claims 22
- 102000039446 nucleic acids Human genes 0.000 claims 22
- 239000000523 sample Substances 0.000 claims 19
- 238000000034 method Methods 0.000 claims 15
- 230000000295 complement effect Effects 0.000 claims 8
- 108010042407 Endonucleases Proteins 0.000 claims 7
- 102000004533 Endonucleases Human genes 0.000 claims 7
- 238000009396 hybridization Methods 0.000 claims 5
- 238000010791 quenching Methods 0.000 claims 5
- 230000000171 quenching effect Effects 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 238000002844 melting Methods 0.000 claims 4
- 230000008018 melting Effects 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 238000005382 thermal cycling Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 241000701806 Human papillomavirus Species 0.000 claims 1
- 241000341655 Human papillomavirus type 16 Species 0.000 claims 1
- 241000701784 Human papillomavirus type 58 Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000010494 dissociation reaction Methods 0.000 claims 1
- 230000005593 dissociations Effects 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 239000001022 rhodamine dye Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000001018 xanthene dye Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26359609P | 2009-11-23 | 2009-11-23 | |
| US61/263,596 | 2009-11-23 | ||
| PCT/US2010/057781 WO2011063388A2 (en) | 2009-11-23 | 2010-11-23 | Assay method for target nucleic acid by signal amplification using probe hybridization and restriction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013511292A JP2013511292A (ja) | 2013-04-04 |
| JP2013511292A5 true JP2013511292A5 (https=) | 2014-01-23 |
Family
ID=44060410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012541159A Withdrawn JP2013511292A (ja) | 2009-11-23 | 2010-11-23 | プローブハイブリダイゼーションおよび制限を使用したシグナル増幅による標的核酸のアッセイ方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8916349B2 (https=) |
| EP (1) | EP2504453A4 (https=) |
| JP (1) | JP2013511292A (https=) |
| CN (1) | CN102686747A (https=) |
| WO (1) | WO2011063388A2 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2473631A4 (en) * | 2009-08-31 | 2013-06-05 | Univ Alberta | BINDING-INDUCED HAIR NEEDLE DETECTION SYSTEM |
| US9603949B2 (en) | 2011-09-01 | 2017-03-28 | University Of Iowa Research Foundation | Oligonucleotide-based probes for detection of bacterial nucleases |
| SG11201404899VA (en) | 2012-02-14 | 2014-10-30 | Univ Cornell | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions |
| GB201217770D0 (en) * | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Biological probes and the use thereof |
| GB201310823D0 (en) * | 2013-06-18 | 2013-07-31 | Lgc Ltd | Fluorophore-based oligonucleotide probes with a universal element |
| GB2520763A (en) * | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Nucleic acid probe and method of detecting genomic fragments |
| US10619219B2 (en) | 2014-02-07 | 2020-04-14 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
| WO2015193483A1 (en) * | 2014-06-19 | 2015-12-23 | Multiplicom N.V. | Restriction mediated quantitative polymerase chain reactions |
| EP3180449B1 (en) * | 2014-08-11 | 2019-10-09 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| US20180179576A1 (en) * | 2015-03-02 | 2018-06-28 | The Regents Of The University Of California | Homogeneous entropy-driven biomolecular assay (heba) |
| KR20180053679A (ko) * | 2015-09-03 | 2018-05-23 | 나노스트링 테크놀로지스, 인크. | 단일 뉴클레오타이드 해상도를 가진 다가 프로브 |
| ITUB20155706A1 (it) * | 2015-11-18 | 2017-05-18 | Fondazione St Italiano Tecnologia | Procedimento di rivelazione di acidi nucleici e relativo kit. |
| ITUB20155686A1 (it) * | 2015-11-18 | 2017-05-18 | Fondazione St Italiano Tecnologia | Procedimento di rivelazione di acidi nucleici e relativo kit. |
| CN109790566A (zh) * | 2016-09-20 | 2019-05-21 | 贝斯4创新公司 | 单核苷酸检测方法及相关的探针 |
| US11459603B2 (en) * | 2017-05-23 | 2022-10-04 | Rutgers, The State University Of New Jersey | Target mediated in situ signal amplification with dual interacting hairpin probes |
| US20190023675A1 (en) * | 2017-07-20 | 2019-01-24 | Eastman Chemical Company | Method for producing purified dialkyl-furan-2,5-dicarboxylate |
| EP3688181A4 (en) | 2017-09-29 | 2021-06-16 | University of Iowa Research Foundation | COMPOSITIONS AND METHOD OF DETECTING DIGITAL NUCLEASES |
| EP4162068A4 (en) * | 2020-06-04 | 2024-10-02 | Logicink Corporation | MULTIPLE DETECTION OF NUCLEIC ACID MARKERS AND SMALL MOLECULES |
| CN111944928B (zh) * | 2020-09-01 | 2024-05-31 | 湖南工程学院 | 基于三链dna结构的多维立体网状杂交链式信号放大的检测eb病毒的序列组合和方法 |
| CN114002200B (zh) * | 2021-11-01 | 2024-05-07 | 中国科学院苏州纳米技术与纳米仿生研究所 | 近红外二区激活型探针及其应用 |
| US20260078430A1 (en) * | 2022-09-08 | 2026-03-19 | Advanced Cell Diagnostics, Inc. | Methods for detecting target nucleic acids using rna blocking molecules |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4766062A (en) | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5846726A (en) | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5935791A (en) | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| WO1999037806A2 (en) * | 1998-01-27 | 1999-07-29 | Cytocell Limited | Modified nucleic acid probes and uses thereof |
| US6316200B1 (en) | 2000-06-08 | 2001-11-13 | Becton, Dickinson And Company | Probes and methods for detection of nucleic acids |
| AU1728001A (en) | 1999-11-29 | 2001-06-04 | Gamida Sense Diagnostics Ltd. | Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample |
| US7361469B2 (en) | 2004-08-13 | 2008-04-22 | Stratagene California | Dual labeled fluorescent probes |
| JP2007075023A (ja) * | 2005-09-15 | 2007-03-29 | Shimadzu Corp | 蛍光共鳴エネルギー移動法を用いた遺伝子多型検出法及びキット |
| JP5665272B2 (ja) * | 2005-10-07 | 2015-02-04 | スピーデクス ピーティーワイ リミテッド | 多成分核酸酵素およびそれらの使用方法 |
| EP1969140A2 (en) | 2005-11-23 | 2008-09-17 | DiaSorin S.p.A. | Reagents and method for simultaneous nucleic acid amplification and detection |
| NZ548731A (en) * | 2006-07-24 | 2008-12-24 | Zygem Corp Ltd | Isothermal detection methods and uses thereof |
| CN101215600B (zh) * | 2007-01-04 | 2012-08-15 | 中国科学院广州生物医药与健康研究院 | 核酸目的序列的检测探针及检测方法 |
| WO2009055366A1 (en) * | 2007-10-22 | 2009-04-30 | Translational Genomics Research Institute (Tgen) | Method and kit used in performing high resolution genomic profiling of clonal cell populations in heterogeneous tissues |
| CN101250593B (zh) | 2008-02-03 | 2011-01-26 | 广州益善生物技术有限公司 | 乳头瘤病毒检测与分型方法及其液相芯片 |
| CN101328498B (zh) * | 2008-06-11 | 2010-10-27 | 北京大学 | 一种通用、实时的核酸酶荧光检测方法 |
| US20110171649A1 (en) * | 2008-09-10 | 2011-07-14 | Igor Kutyavin | Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v |
| CA2755615A1 (en) * | 2009-03-18 | 2010-09-23 | Sequenom, Inc. | Use of thermostable endonucleases for generating reporter molecules |
-
2010
- 2010-11-23 US US13/511,269 patent/US8916349B2/en active Active
- 2010-11-23 EP EP10832359.3A patent/EP2504453A4/en not_active Withdrawn
- 2010-11-23 JP JP2012541159A patent/JP2013511292A/ja not_active Withdrawn
- 2010-11-23 CN CN2010800606469A patent/CN102686747A/zh active Pending
- 2010-11-23 WO PCT/US2010/057781 patent/WO2011063388A2/en not_active Ceased
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