CN102686747A - 使用探针杂交和限制性通过信号扩大而检测靶核酸的方法 - Google Patents

使用探针杂交和限制性通过信号扩大而检测靶核酸的方法 Download PDF

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CN102686747A
CN102686747A CN2010800606469A CN201080060646A CN102686747A CN 102686747 A CN102686747 A CN 102686747A CN 2010800606469 A CN2010800606469 A CN 2010800606469A CN 201080060646 A CN201080060646 A CN 201080060646A CN 102686747 A CN102686747 A CN 102686747A
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sequence
probe
oligonucleotide
nucleic acid
oligonucleotide probe
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J·纳德乌
T·赫雷尔
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Becton Dickinson and Co
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Becton Dickinson and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
  • Biotechnology (AREA)
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  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2010800606469A 2009-11-23 2010-11-23 使用探针杂交和限制性通过信号扩大而检测靶核酸的方法 Pending CN102686747A (zh)

Applications Claiming Priority (3)

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US26359609P 2009-11-23 2009-11-23
US61/263,596 2009-11-23
PCT/US2010/057781 WO2011063388A2 (en) 2009-11-23 2010-11-23 Assay method for target nucleic acid by signal amplification using probe hybridization and restriction

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CN102686747A true CN102686747A (zh) 2012-09-19

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US (1) US8916349B2 (https=)
EP (1) EP2504453A4 (https=)
JP (1) JP2013511292A (https=)
CN (1) CN102686747A (https=)
WO (1) WO2011063388A2 (https=)

Cited By (4)

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CN105555969A (zh) * 2013-06-18 2016-05-04 Lgc有限公司 具有通用元件的基于荧光团的寡核苷酸探针
CN108707652A (zh) * 2013-12-02 2018-10-26 苏州新波生物技术有限公司 核酸探针和检测基因组片段的方法
CN110730825A (zh) * 2017-05-23 2020-01-24 新泽西鲁特格斯州立大学 用双相互作用发夹探针进行的靶标介导的原位信号放大
CN113637728A (zh) * 2014-08-11 2021-11-12 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针

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EP2473631A4 (en) * 2009-08-31 2013-06-05 Univ Alberta BINDING-INDUCED HAIR NEEDLE DETECTION SYSTEM
US9603949B2 (en) 2011-09-01 2017-03-28 University Of Iowa Research Foundation Oligonucleotide-based probes for detection of bacterial nucleases
SG11201404899VA (en) 2012-02-14 2014-10-30 Univ Cornell Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
GB201217770D0 (en) * 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
US10619219B2 (en) 2014-02-07 2020-04-14 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
WO2015193483A1 (en) * 2014-06-19 2015-12-23 Multiplicom N.V. Restriction mediated quantitative polymerase chain reactions
US20180179576A1 (en) * 2015-03-02 2018-06-28 The Regents Of The University Of California Homogeneous entropy-driven biomolecular assay (heba)
KR20180053679A (ko) * 2015-09-03 2018-05-23 나노스트링 테크놀로지스, 인크. 단일 뉴클레오타이드 해상도를 가진 다가 프로브
ITUB20155706A1 (it) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Procedimento di rivelazione di acidi nucleici e relativo kit.
ITUB20155686A1 (it) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Procedimento di rivelazione di acidi nucleici e relativo kit.
CN109790566A (zh) * 2016-09-20 2019-05-21 贝斯4创新公司 单核苷酸检测方法及相关的探针
US20190023675A1 (en) * 2017-07-20 2019-01-24 Eastman Chemical Company Method for producing purified dialkyl-furan-2,5-dicarboxylate
EP3688181A4 (en) 2017-09-29 2021-06-16 University of Iowa Research Foundation COMPOSITIONS AND METHOD OF DETECTING DIGITAL NUCLEASES
EP4162068A4 (en) * 2020-06-04 2024-10-02 Logicink Corporation MULTIPLE DETECTION OF NUCLEIC ACID MARKERS AND SMALL MOLECULES
CN111944928B (zh) * 2020-09-01 2024-05-31 湖南工程学院 基于三链dna结构的多维立体网状杂交链式信号放大的检测eb病毒的序列组合和方法
CN114002200B (zh) * 2021-11-01 2024-05-07 中国科学院苏州纳米技术与纳米仿生研究所 近红外二区激活型探针及其应用
US20260078430A1 (en) * 2022-09-08 2026-03-19 Advanced Cell Diagnostics, Inc. Methods for detecting target nucleic acids using rna blocking molecules

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JP2007075023A (ja) * 2005-09-15 2007-03-29 Shimadzu Corp 蛍光共鳴エネルギー移動法を用いた遺伝子多型検出法及びキット
CN101215600A (zh) * 2007-01-04 2008-07-09 中国科学院广州生物医药与健康研究院 核酸目的序列的检测探针及检测方法
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CN101328498A (zh) * 2008-06-11 2008-12-24 北京大学 一种通用、实时的核酸酶荧光检测方法
WO2009055366A1 (en) * 2007-10-22 2009-04-30 Translational Genomics Research Institute (Tgen) Method and kit used in performing high resolution genomic profiling of clonal cell populations in heterogeneous tissues
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US20040142369A1 (en) * 1999-11-29 2004-07-22 Sara Alajem Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
JP2007075023A (ja) * 2005-09-15 2007-03-29 Shimadzu Corp 蛍光共鳴エネルギー移動法を用いた遺伝子多型検出法及びキット
CN101215600A (zh) * 2007-01-04 2008-07-09 中国科学院广州生物医药与健康研究院 核酸目的序列的检测探针及检测方法
WO2009055366A1 (en) * 2007-10-22 2009-04-30 Translational Genomics Research Institute (Tgen) Method and kit used in performing high resolution genomic profiling of clonal cell populations in heterogeneous tissues
CN101250593A (zh) * 2008-02-03 2008-08-27 广州益善生物技术有限公司 乳头瘤病毒检测与分型方法及其液相芯片
CN101328498A (zh) * 2008-06-11 2008-12-24 北京大学 一种通用、实时的核酸酶荧光检测方法
WO2010030716A1 (en) * 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105555969A (zh) * 2013-06-18 2016-05-04 Lgc有限公司 具有通用元件的基于荧光团的寡核苷酸探针
CN108707652A (zh) * 2013-12-02 2018-10-26 苏州新波生物技术有限公司 核酸探针和检测基因组片段的方法
CN108707652B (zh) * 2013-12-02 2022-04-15 苏州新波生物技术有限公司 核酸探针和检测基因组片段的方法
CN113637728A (zh) * 2014-08-11 2021-11-12 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针
CN113637728B (zh) * 2014-08-11 2025-04-29 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针
CN110730825A (zh) * 2017-05-23 2020-01-24 新泽西鲁特格斯州立大学 用双相互作用发夹探针进行的靶标介导的原位信号放大
CN110730825B (zh) * 2017-05-23 2023-08-11 新泽西鲁特格斯州立大学 用双相互作用发夹探针进行的靶标介导的原位信号放大

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EP2504453A2 (en) 2012-10-03
US20120322067A1 (en) 2012-12-20
EP2504453A4 (en) 2013-07-17
JP2013511292A (ja) 2013-04-04
WO2011063388A3 (en) 2011-10-20
US8916349B2 (en) 2014-12-23
WO2011063388A2 (en) 2011-05-26

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Application publication date: 20120919