JP2013500021A5 - - Google Patents
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- Publication number
- JP2013500021A5 JP2013500021A5 JP2012521908A JP2012521908A JP2013500021A5 JP 2013500021 A5 JP2013500021 A5 JP 2013500021A5 JP 2012521908 A JP2012521908 A JP 2012521908A JP 2012521908 A JP2012521908 A JP 2012521908A JP 2013500021 A5 JP2013500021 A5 JP 2013500021A5
- Authority
- JP
- Japan
- Prior art keywords
- platform
- polynucleotide molecule
- reagent
- pyrosequencing
- rotatable platform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 23
- 239000002157 polynucleotide Substances 0.000 claims 18
- 102000040430 polynucleotide Human genes 0.000 claims 18
- 108091033319 polynucleotide Proteins 0.000 claims 18
- 239000003153 chemical reaction reagent Substances 0.000 claims 10
- 238000012175 pyrosequencing Methods 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 238000005406 washing Methods 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 claims 2
- 238000000576 coating method Methods 0.000 claims 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical group [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 2
- -1 polyethylene Polymers 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 102000007347 Apyrase Human genes 0.000 claims 1
- 108010007730 Apyrase Proteins 0.000 claims 1
- 108090001008 Avidin Proteins 0.000 claims 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims 1
- 239000004698 Polyethylene Substances 0.000 claims 1
- 239000004743 Polypropylene Substances 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 claims 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 claims 1
- 238000005411 Van der Waals force Methods 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 229920005669 high impact polystyrene Polymers 0.000 claims 1
- 239000004797 high-impact polystyrene Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 229920003023 plastic Polymers 0.000 claims 1
- 229920000515 polycarbonate Polymers 0.000 claims 1
- 239000004417 polycarbonate Substances 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 229920001155 polypropylene Polymers 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000010453 quartz Substances 0.000 claims 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 1
- 239000002699 waste material Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2009903546A AU2009903546A0 (en) | 2009-07-29 | Method and apparatus for conducting an assay | |
| AU2009903546 | 2009-07-29 | ||
| PCT/AU2010/000953 WO2011011823A1 (en) | 2009-07-29 | 2010-07-29 | Method and apparatus for conducting an assay |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013500021A JP2013500021A (ja) | 2013-01-07 |
| JP2013500021A5 true JP2013500021A5 (https=) | 2013-09-05 |
| JP5795313B2 JP5795313B2 (ja) | 2015-10-14 |
Family
ID=43528635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012521908A Expired - Fee Related JP5795313B2 (ja) | 2009-07-29 | 2010-07-29 | アッセイを行うための方法及び装置 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8921040B2 (https=) |
| EP (1) | EP2459750A1 (https=) |
| JP (1) | JP5795313B2 (https=) |
| KR (1) | KR20120089640A (https=) |
| CN (1) | CN102712950B (https=) |
| AU (1) | AU2010278668B2 (https=) |
| CA (1) | CA2769006A1 (https=) |
| MX (1) | MX2012001214A (https=) |
| NZ (1) | NZ598226A (https=) |
| SG (1) | SG177733A1 (https=) |
| WO (1) | WO2011011823A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012200646B2 (en) * | 2012-02-03 | 2014-09-25 | Pyrobett Pte Ltd. | Method and apparatus for conducting an assay |
| PT2809799T (pt) * | 2012-02-03 | 2017-06-05 | Pyrobett Pte Ltd | Plataforma giratória para realizar sequenciamento de ácido nucleico |
| US9146248B2 (en) | 2013-03-14 | 2015-09-29 | Intelligent Bio-Systems, Inc. | Apparatus and methods for purging flow cells in nucleic acid sequencing instruments |
| US9591268B2 (en) | 2013-03-15 | 2017-03-07 | Qiagen Waltham, Inc. | Flow cell alignment methods and systems |
| US10222304B2 (en) * | 2013-03-15 | 2019-03-05 | Massachusetts Institute Of Technology | Deposition and imaging of particles on planar substrates |
| WO2017210556A1 (en) * | 2016-06-02 | 2017-12-07 | Integrated Nano-Technologies, Inc. | System and method for optimizing heat transfer for target amplification within a diagnostic assay system |
| US11499962B2 (en) | 2017-11-17 | 2022-11-15 | Ultima Genomics, Inc. | Methods and systems for analyte detection and analysis |
| US10267790B1 (en) | 2017-11-17 | 2019-04-23 | Ultima Genomics, Inc. | Systems for biological sample processing and analysis |
| US12589394B2 (en) | 2018-11-13 | 2026-03-31 | A. National Research Council of Canada | World-to-chip automated interface for centrifugal microfluidic platforms |
| US12239980B2 (en) | 2018-12-07 | 2025-03-04 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US10512911B1 (en) * | 2018-12-07 | 2019-12-24 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US10852518B1 (en) | 2019-03-14 | 2020-12-01 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10900078B2 (en) | 2019-03-14 | 2021-01-26 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US11118223B2 (en) | 2019-03-14 | 2021-09-14 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10830703B1 (en) | 2019-03-14 | 2020-11-10 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4971903A (en) | 1988-03-25 | 1990-11-20 | Edward Hyman | Pyrophosphate-based method and apparatus for sequencing nucleic acids |
| GB9210168D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Method of sequencing dna |
| US5639428A (en) * | 1994-07-19 | 1997-06-17 | Becton Dickinson And Company | Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US6258533B1 (en) * | 1996-11-01 | 2001-07-10 | The University Of Iowa Research Foundation | Iterative and regenerative DNA sequencing method |
| AUPO652997A0 (en) * | 1997-04-30 | 1997-05-29 | Kindconi Pty Limited | Temperature cycling device and method |
| US5922617A (en) * | 1997-11-12 | 1999-07-13 | Functional Genetics, Inc. | Rapid screening assay methods and devices |
| US20020177144A1 (en) * | 1997-12-30 | 2002-11-28 | Jose Remacle | Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc |
| GB9828785D0 (en) * | 1998-12-30 | 1999-02-17 | Amersham Pharm Biotech Ab | Sequencing systems |
| DE60045917D1 (de) * | 1999-02-23 | 2011-06-16 | Caliper Life Sciences Inc | Sequenzierung durch inkorporation |
| US6884395B2 (en) * | 2000-05-12 | 2005-04-26 | Gyros Ab | Integrated microfluidic disc |
| KR20020063359A (ko) * | 2001-01-27 | 2002-08-03 | 일렉트론 바이오 (주) | 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치 |
| US20040234971A1 (en) * | 2001-02-01 | 2004-11-25 | Joany Jackman | Diagnosis of pathogen infections using mass spectral analysis of immune system modulators in post-exposure biological samples |
| EP1493014A2 (en) * | 2001-04-11 | 2005-01-05 | Burstein Technologies, Inc. | Multi-parameter assays including analysis discs and methods relating thereto |
| AU2002952797A0 (en) | 2002-11-20 | 2002-12-05 | Bio-Molecular Holdings Pty Limited | Centrifugal device and method using same |
| WO2005003375A2 (en) * | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| EP2290106B1 (en) * | 2004-03-08 | 2018-01-03 | Rubicon Genomics, Inc. | Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| CA2559778C (en) | 2004-03-26 | 2011-07-05 | Infectio Recherche Inc. | Removable microfluidic flow cell |
| US8377377B2 (en) | 2005-05-24 | 2013-02-19 | Lee H. Angros | In situ heat induced antigen recovery and staining apparatus and method |
| JP5030110B2 (ja) * | 2005-12-21 | 2012-09-19 | サムスン エレクトロニクス カンパニー リミテッド | バイオメモリディスクドライブ装置及びそれを用いた分析方法 |
| US20140193807A1 (en) * | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
| US7727723B2 (en) * | 2006-04-18 | 2010-06-01 | Advanced Liquid Logic, Inc. | Droplet-based pyrosequencing |
| ATE540750T1 (de) * | 2006-05-11 | 2012-01-15 | Raindance Technologies Inc | Mikrofluidische vorrichtung und verfahren |
| US8481259B2 (en) * | 2007-02-05 | 2013-07-09 | Intelligent Bio-Systems, Inc. | Methods and devices for sequencing nucleic acids in smaller batches |
| US8470164B2 (en) * | 2008-06-25 | 2013-06-25 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US20100301398A1 (en) * | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| WO2010077859A2 (en) * | 2008-12-15 | 2010-07-08 | Advanced Liquid Logic, Inc. | Nucleic acid amplification and sequencing on a droplet actuator |
| US8574835B2 (en) * | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| US20130288254A1 (en) * | 2009-08-13 | 2013-10-31 | Advanced Liquid Logic, Inc. | Droplet Actuator and Droplet-Based Techniques |
| US8597882B2 (en) | 2012-02-03 | 2013-12-03 | Pyrobett Pte. Ltd. | Method and apparatus for conducting an assay |
| EP2852682B1 (en) * | 2012-05-21 | 2017-10-04 | Fluidigm Corporation | Single-particle analysis of particle populations |
-
2010
- 2010-07-29 MX MX2012001214A patent/MX2012001214A/es active IP Right Grant
- 2010-07-29 AU AU2010278668A patent/AU2010278668B2/en not_active Ceased
- 2010-07-29 NZ NZ598226A patent/NZ598226A/xx not_active IP Right Cessation
- 2010-07-29 WO PCT/AU2010/000953 patent/WO2011011823A1/en not_active Ceased
- 2010-07-29 US US13/386,834 patent/US8921040B2/en not_active Expired - Fee Related
- 2010-07-29 JP JP2012521908A patent/JP5795313B2/ja not_active Expired - Fee Related
- 2010-07-29 EP EP10803732A patent/EP2459750A1/en not_active Withdrawn
- 2010-07-29 CA CA2769006A patent/CA2769006A1/en not_active Abandoned
- 2010-07-29 CN CN201080042696.4A patent/CN102712950B/zh not_active Expired - Fee Related
- 2010-07-29 KR KR1020127005528A patent/KR20120089640A/ko not_active Ceased
- 2010-07-29 SG SG2012004800A patent/SG177733A1/en unknown
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