MX353148B - Plataforma giratoria para realizar secuenciacion de acidos nucleicos. - Google Patents
Plataforma giratoria para realizar secuenciacion de acidos nucleicos.Info
- Publication number
- MX353148B MX353148B MX2014009367A MX2014009367A MX353148B MX 353148 B MX353148 B MX 353148B MX 2014009367 A MX2014009367 A MX 2014009367A MX 2014009367 A MX2014009367 A MX 2014009367A MX 353148 B MX353148 B MX 353148B
- Authority
- MX
- Mexico
- Prior art keywords
- support surface
- nucleic acid
- well
- acid sequencing
- platform
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 238000012163 sequencing technique Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000012175 pyrosequencing Methods 0.000 abstract 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
- B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
La presente invención se refiere a métodos y aparatos para conducir la secuenciación de ácido nucleico. El método comprende las etapas de proporcionar una plataforma que tiene al menos un pozo para contener al menos una superficie de soporte, y proporcionar al menos una superficie de soporte dentro de cada pozo, en donde la superficie de soporte se adapta para inmovilizar una primera pareja de unión o inmovilizar selectivamente una segunda pareja de unión. El método comprende además las etapas de unir o inmovilizar la primera o segunda pareja de unión a la superficie de soporte y distribuir un reactivo en cada pozo desde un punto externo de dicha plataforma, en donde después de la etapa de distribución la plataforma se gira lo suficiente de tal manera que cualquier reactivo residual o sin reaccionar se elimine sustancialmente de forma centrífuga de cada pozo y/o de cada superficie de soporte, en donde durante la rotación cada superficie de soporte se mantiene dentro de cada pozo. La invención también se refiere a equipos y usos de los equipos para conducir la secuenciación del ácido nucleico. En particular, la invención se ha desarrollado principalmente para utilizarse en la secuenciación del ácido nucleico mediante pirosecuenciación, sin embargo, la invención no se limita a este campo.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/AU2012/000089 WO2013113054A1 (en) | 2012-02-03 | 2012-02-03 | Rotatable platform for conducting nucleic acid sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014009367A MX2014009367A (es) | 2015-09-22 |
| MX353148B true MX353148B (es) | 2017-12-19 |
Family
ID=48904296
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014009367A MX353148B (es) | 2012-02-03 | 2012-02-03 | Plataforma giratoria para realizar secuenciacion de acidos nucleicos. |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP2809799B1 (es) |
| JP (1) | JP6047586B2 (es) |
| KR (1) | KR101916989B1 (es) |
| CN (1) | CN104379762B (es) |
| DK (1) | DK2809799T3 (es) |
| ES (1) | ES2632423T3 (es) |
| HU (1) | HUE032997T2 (es) |
| IN (1) | IN2014DN07114A (es) |
| MX (1) | MX353148B (es) |
| PH (1) | PH12014501746A1 (es) |
| PL (1) | PL2809799T3 (es) |
| PT (1) | PT2809799T (es) |
| RU (1) | RU2601139C2 (es) |
| SG (1) | SG11201404440RA (es) |
| WO (1) | WO2013113054A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2809767B1 (en) * | 2012-02-03 | 2017-11-08 | Axxin Pty Ltd | Nucleic acid amplification and detection apparatus |
| EP2864503B1 (en) | 2012-06-26 | 2019-10-09 | Axxin Pty Ltd | Nucleic acid amplification and detection kit |
| ES2809600T3 (es) | 2014-11-14 | 2021-03-04 | Axxin Pty Ltd | Conjunto de recogida y almacenamiento de muestras biológicas |
| WO2016130962A1 (en) | 2015-02-13 | 2016-08-18 | Abbott Laboratories | Automated storage modules for diagnostic analyzer liquids and related systems and methods |
| WO2017011862A1 (en) | 2015-07-17 | 2017-01-26 | Axxin Pty Ltd | Diagnostic test assembly, apparatus, method |
| KR102651024B1 (ko) | 2017-09-27 | 2024-03-26 | 액신 피티와이 엘티디 | 진단 테스트 시스템 및 방법 |
| CN108142214A (zh) * | 2018-01-03 | 2018-06-12 | 河南天蚕生物科技有限公司 | 一种立式虫草菌扩培装置及其扩培瓶 |
| CN108325761B (zh) * | 2018-02-10 | 2018-12-14 | 东台市苏泰饲料有限公司 | 一种饲料用酶制备装置 |
| CN108855600B (zh) * | 2018-06-22 | 2019-12-24 | 江西振彪建筑工程有限公司 | 一种建材石英砂磁选用旋转式送料设备 |
| CN111558402B (zh) * | 2020-03-10 | 2021-05-11 | 青岛英赛特生物科技有限公司 | 一种气压驱动的离心式微流控检测芯片 |
| CN112246140B (zh) * | 2020-10-12 | 2021-07-27 | 广州爱索达生物医药技术有限公司 | 一种基于肿瘤诊断及预测的外泌体中rna检测装置 |
| US20220403466A1 (en) * | 2021-06-17 | 2022-12-22 | Mgi Tech Co., Ltd. | Sequencing systems and methods utilizing curved imaging paths on rotating substrates |
| CN118416976A (zh) * | 2024-05-27 | 2024-08-02 | 北京京东方技术开发有限公司 | 微流控芯片和回收不同片段大小核酸的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE556149T1 (de) * | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| AU2002952797A0 (en) * | 2002-11-20 | 2002-12-05 | Bio-Molecular Holdings Pty Limited | Centrifugal device and method using same |
| RU2328531C2 (ru) * | 2006-06-09 | 2008-07-10 | Всероссийский государственный научно-исследовательский институт животноводства (ВИЖ) | Способ диагностики аллелей t30n, s52g, i199v и r200q гена prkag3 |
| NZ598226A (en) * | 2009-07-29 | 2013-09-27 | Pyrobett Pte Ltd | Method and apparatus for conducting an assay |
-
2012
- 2012-02-03 MX MX2014009367A patent/MX353148B/es active IP Right Grant
- 2012-02-03 IN IN7114DEN2014 patent/IN2014DN07114A/en unknown
- 2012-02-03 JP JP2014555038A patent/JP6047586B2/ja not_active Expired - Fee Related
- 2012-02-03 PT PT128675196T patent/PT2809799T/pt unknown
- 2012-02-03 KR KR1020147024457A patent/KR101916989B1/ko not_active Expired - Fee Related
- 2012-02-03 HU HUE12867519A patent/HUE032997T2/en unknown
- 2012-02-03 SG SG11201404440RA patent/SG11201404440RA/en unknown
- 2012-02-03 ES ES12867519.6T patent/ES2632423T3/es active Active
- 2012-02-03 EP EP12867519.6A patent/EP2809799B1/en active Active
- 2012-02-03 PL PL12867519T patent/PL2809799T3/pl unknown
- 2012-02-03 DK DK12867519.6T patent/DK2809799T3/en active
- 2012-02-03 WO PCT/AU2012/000089 patent/WO2013113054A1/en not_active Ceased
- 2012-02-03 CN CN201280072351.2A patent/CN104379762B/zh not_active Expired - Fee Related
- 2012-02-03 RU RU2014134097/10A patent/RU2601139C2/ru active
-
2014
- 2014-08-01 PH PH12014501746A patent/PH12014501746A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2809799B1 (en) | 2017-04-05 |
| HUE032997T2 (en) | 2017-11-28 |
| KR101916989B1 (ko) | 2018-11-08 |
| WO2013113054A1 (en) | 2013-08-08 |
| PH12014501746B1 (en) | 2014-11-10 |
| RU2601139C2 (ru) | 2016-10-27 |
| EP2809799A1 (en) | 2014-12-10 |
| CN104379762A (zh) | 2015-02-25 |
| JP2015506690A (ja) | 2015-03-05 |
| PL2809799T3 (pl) | 2017-09-29 |
| NZ628992A (en) | 2016-09-30 |
| CN104379762B (zh) | 2016-08-24 |
| KR20150014908A (ko) | 2015-02-09 |
| DK2809799T3 (en) | 2017-07-17 |
| SG11201404440RA (en) | 2014-08-28 |
| IN2014DN07114A (es) | 2015-04-24 |
| PH12014501746A1 (en) | 2014-11-10 |
| ES2632423T3 (es) | 2017-09-13 |
| RU2014134097A (ru) | 2016-03-27 |
| JP6047586B2 (ja) | 2016-12-21 |
| MX2014009367A (es) | 2015-09-22 |
| PT2809799T (pt) | 2017-06-05 |
| EP2809799A4 (en) | 2015-10-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |