JP2013500015A - シンドビスウイルスエンベロープ糖タンパク質により偽型化したレンチウイルスベクター - Google Patents

Info

Publication number

JP2013500015A

JP2013500015A JP2012521778A JP2012521778A JP2013500015A JP 2013500015 A JP2013500015 A JP 2013500015A JP 2012521778 A JP2012521778 A JP 2012521778A JP 2012521778 A JP2012521778 A JP 2012521778A JP 2013500015 A JP2013500015 A JP 2013500015A

Authority

JP

Japan

Prior art keywords

sequence

lentiviral vector

cells

virus

seq

Prior art date

2009-07-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Withdrawn

Links

• Espacenet
• Global Dossier
• Discuss

• 239000013598 vector Substances 0.000 title claims abstract description 223
• 241000710960 Sindbis virus Species 0.000 title claims description 51
• 108010003533 Viral Envelope Proteins Proteins 0.000 title claims description 25
• 102100021696 Syncytin-1 Human genes 0.000 title 1
• 239000002245 particle Substances 0.000 claims abstract description 144
• 210000004443 dendritic cell Anatomy 0.000 claims abstract description 117
• 102000003886 Glycoproteins Human genes 0.000 claims abstract description 65
• 108090000288 Glycoproteins Proteins 0.000 claims abstract description 65
• 208000015181 infectious disease Diseases 0.000 claims abstract description 30
• 101500008206 Sindbis virus Spike glycoprotein E2 Proteins 0.000 claims abstract description 19
• 210000004027 cell Anatomy 0.000 claims description 233
• 241000700605 Viruses Species 0.000 claims description 115
• 239000000427 antigen Substances 0.000 claims description 109
• 108091007433 antigens Proteins 0.000 claims description 108
• 102000036639 antigens Human genes 0.000 claims description 108
• 108090000623 proteins and genes Proteins 0.000 claims description 85
• 150000001413 amino acids Chemical class 0.000 claims description 67
• 229940024606 amino acid Drugs 0.000 claims description 62
• 235000001014 amino acid Nutrition 0.000 claims description 62
• 238000012217 deletion Methods 0.000 claims description 52
• 230000037430 deletion Effects 0.000 claims description 52
• 238000000034 method Methods 0.000 claims description 52
• 150000007523 nucleic acids Chemical class 0.000 claims description 47
• 235000018102 proteins Nutrition 0.000 claims description 45
• 102000004169 proteins and genes Human genes 0.000 claims description 45
• 102000039446 nucleic acids Human genes 0.000 claims description 43
• 108020004707 nucleic acids Proteins 0.000 claims description 43
• 230000035772 mutation Effects 0.000 claims description 41
• 108010061833 Integrases Proteins 0.000 claims description 38
• 108010037897 DC-specific ICAM-3 grabbing nonintegrin Proteins 0.000 claims description 34
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 32
• 238000004806 packaging method and process Methods 0.000 claims description 28
• 235000018977 lysine Nutrition 0.000 claims description 27
• 206010028980 Neoplasm Diseases 0.000 claims description 26
• 235000013922 glutamic acid Nutrition 0.000 claims description 25
• 239000004220 glutamic acid Substances 0.000 claims description 25
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 24
• 108010076039 Polyproteins Proteins 0.000 claims description 23
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 22
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 21
• 239000004472 Lysine Substances 0.000 claims description 21
• 238000006467 substitution reaction Methods 0.000 claims description 21
• 230000008859 change Effects 0.000 claims description 17
• 239000004471 Glycine Substances 0.000 claims description 14
• 239000013604 expression vector Substances 0.000 claims description 14
• 241001465754 Metazoa Species 0.000 claims description 13
• 238000011282 treatment Methods 0.000 claims description 12
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 11
• 238000000338 in vitro Methods 0.000 claims description 11
• 230000001225 therapeutic effect Effects 0.000 claims description 11
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 10
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 10
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 10
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 10
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 10
• 229960000310 isoleucine Drugs 0.000 claims description 10
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 10
• 102220036548 rs140382474 Human genes 0.000 claims description 10
• 239000004474 valine Substances 0.000 claims description 10
• 235000003704 aspartic acid Nutrition 0.000 claims description 8
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 8
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 7
• 235000004279 alanine Nutrition 0.000 claims description 7
• 108020001507 fusion proteins Proteins 0.000 claims description 7
• 102000037865 fusion proteins Human genes 0.000 claims description 7
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims description 7
• 238000003780 insertion Methods 0.000 claims description 6
• 230000037431 insertion Effects 0.000 claims description 6
• 210000004779 membrane envelope Anatomy 0.000 claims description 6
• 101710177291 Gag polyprotein Proteins 0.000 claims description 5
• 101001051709 Homo sapiens Ribosomal protein S6 kinase-related protein Proteins 0.000 claims description 5
• 102100024914 Ribosomal protein S6 kinase-related protein Human genes 0.000 claims description 5
• 108010089520 pol Gene Products Proteins 0.000 claims description 5
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
• 238000002156 mixing Methods 0.000 claims description 4
• 101710125418 Major capsid protein Proteins 0.000 claims description 3
• 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
• 229940021993 prophylactic vaccine Drugs 0.000 claims description 2
• 229940021747 therapeutic vaccine Drugs 0.000 claims description 2
• 102100034343 Integrase Human genes 0.000 claims 2
• 230000027455 binding Effects 0.000 abstract description 41
• 229920002971 Heparan sulfate Polymers 0.000 abstract description 22
• 230000002829 reductive effect Effects 0.000 abstract description 13
• 108090000765 processed proteins & peptides Proteins 0.000 description 205
• 102000004196 processed proteins & peptides Human genes 0.000 description 193
• 229920001184 polypeptide Polymers 0.000 description 187
• 230000003612 virological effect Effects 0.000 description 84
• 102100034349 Integrase Human genes 0.000 description 76
• 230000014509 gene expression Effects 0.000 description 71
• 239000002671 adjuvant Substances 0.000 description 41
• 239000013603 viral vector Substances 0.000 description 41
• 239000013612 plasmid Substances 0.000 description 37
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 33
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 33
• 239000005090 green fluorescent protein Substances 0.000 description 33
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 31
• 239000000047 product Substances 0.000 description 29
• 239000003623 enhancer Substances 0.000 description 28
• 101710121417 Envelope glycoprotein Proteins 0.000 description 26
• 238000003776 cleavage reaction Methods 0.000 description 26
• 230000028993 immune response Effects 0.000 description 26
• 230000007017 scission Effects 0.000 description 25
• 241000725303 Human immunodeficiency virus Species 0.000 description 24
• 210000001744 T-lymphocyte Anatomy 0.000 description 23
• 102100034922 T-cell surface glycoprotein CD8 alpha chain Human genes 0.000 description 22
• 230000000694 effects Effects 0.000 description 22
• 230000010354 integration Effects 0.000 description 22
• 241000699670 Mus sp. Species 0.000 description 21
• 230000001177 retroviral effect Effects 0.000 description 21
• 238000010361 transduction Methods 0.000 description 20
• 230000026683 transduction Effects 0.000 description 20
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
• 241000701022 Cytomegalovirus Species 0.000 description 18
• -1 aliphatic amino acids Chemical class 0.000 description 18
• 201000010099 disease Diseases 0.000 description 18
• 230000035800 maturation Effects 0.000 description 17
• 239000002609 medium Substances 0.000 description 17
• 230000001105 regulatory effect Effects 0.000 description 17
• 125000001183 hydrocarbyl group Chemical group 0.000 description 16
• 102000040430 polynucleotide Human genes 0.000 description 15
• 108091033319 polynucleotide Proteins 0.000 description 15
• 239000002157 polynucleotide Substances 0.000 description 15
• 239000006228 supernatant Substances 0.000 description 15
• 102000004127 Cytokines Human genes 0.000 description 14
• 108090000695 Cytokines Proteins 0.000 description 14
• 208000035475 disorder Diseases 0.000 description 13
• 238000004519 manufacturing process Methods 0.000 description 13
• 102000004961 Furin Human genes 0.000 description 12
• 108090001126 Furin Proteins 0.000 description 12
• 101710125507 Integrase/recombinase Proteins 0.000 description 12
• 230000006870 function Effects 0.000 description 12
• 108020004414 DNA Proteins 0.000 description 11
• 241000713311 Simian immunodeficiency virus Species 0.000 description 11
• 230000004927 fusion Effects 0.000 description 11
• 238000010186 staining Methods 0.000 description 11
• 102000005593 Endopeptidases Human genes 0.000 description 10
• 108010059378 Endopeptidases Proteins 0.000 description 10
• 102100037850 Interferon gamma Human genes 0.000 description 10
• 108010074328 Interferon-gamma Proteins 0.000 description 10
• 125000000217 alkyl group Chemical group 0.000 description 10
• 238000013459 approach Methods 0.000 description 10
• 238000000684 flow cytometry Methods 0.000 description 10
• 230000001939 inductive effect Effects 0.000 description 10
• 239000007924 injection Substances 0.000 description 10
• 238000002347 injection Methods 0.000 description 10
• 239000000203 mixture Substances 0.000 description 10
• 238000002360 preparation method Methods 0.000 description 10
• 102000005962 receptors Human genes 0.000 description 10
• 108020003175 receptors Proteins 0.000 description 10
• 238000001890 transfection Methods 0.000 description 10
• 241001430294 unidentified retrovirus Species 0.000 description 10
• DRHZYJAUECRAJM-DWSYSWFDSA-N (2s,3s,4s,5r,6r)-6-[[(3s,4s,4ar,6ar,6bs,8r,8ar,12as,14ar,14br)-8a-[(2s,3r,4s,5r,6r)-3-[(2s,3r,4s,5r,6s)-5-[(2s,3r,4s,5r)-4-[(2s,3r,4r)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-3,5-dihydroxyoxan-2-yl]oxy-3,4-dihydroxy-6-methyloxan-2-yl]oxy-5-[(3s,5s, Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@H]5CC(C)(C)CC[C@@]5([C@@H](C[C@@]4(C)[C@]3(C)CC[C@H]2[C@@]1(C=O)C)O)C(=O)O[C@@H]1O[C@H](C)[C@@H]([C@@H]([C@H]1O[C@H]1[C@@H]([C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@](O)(CO)CO3)O)[C@H](O)CO2)O)[C@H](C)O1)O)O)OC(=O)C[C@@H](O)C[C@H](OC(=O)C[C@@H](O)C[C@@H]([C@@H](C)CC)O[C@H]1[C@@H]([C@@H](O)[C@H](CO)O1)O)[C@@H](C)CC)C(O)=O)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O DRHZYJAUECRAJM-DWSYSWFDSA-N 0.000 description 9
• 101710091045 Envelope protein Proteins 0.000 description 9
• 241000713666 Lentivirus Species 0.000 description 9
• 101710188315 Protein X Proteins 0.000 description 9
• 238000004458 analytical method Methods 0.000 description 9
• 125000004432 carbon atom Chemical group C* 0.000 description 9
• 125000003588 lysine group Chemical class [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 9
• 230000007246 mechanism Effects 0.000 description 9
• 239000012528 membrane Substances 0.000 description 9
• 108700011259 MicroRNAs Proteins 0.000 description 8
• 102000014450 RNA Polymerase III Human genes 0.000 description 8
• 108010078067 RNA Polymerase III Proteins 0.000 description 8
• 230000002950 deficient Effects 0.000 description 8
• 239000008194 pharmaceutical composition Substances 0.000 description 8
• 239000013600 plasmid vector Substances 0.000 description 8
• 230000010076 replication Effects 0.000 description 8
• 238000002255 vaccination Methods 0.000 description 8
• 229960005486 vaccine Drugs 0.000 description 8
• 241000710929 Alphavirus Species 0.000 description 7
• 108091026890 Coding region Proteins 0.000 description 7
• 102000009572 RNA Polymerase II Human genes 0.000 description 7
• 108010009460 RNA Polymerase II Proteins 0.000 description 7
• 235000009697 arginine Nutrition 0.000 description 7
• 125000000637 arginyl group Chemical class N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 7
• 230000001580 bacterial effect Effects 0.000 description 7
• 150000001875 compounds Chemical class 0.000 description 7
• 230000002068 genetic effect Effects 0.000 description 7
• 230000003053 immunization Effects 0.000 description 7
• 238000002649 immunization Methods 0.000 description 7
• 230000003834 intracellular effect Effects 0.000 description 7
• 239000002679 microRNA Substances 0.000 description 7
• 210000001519 tissue Anatomy 0.000 description 7
• 238000013518 transcription Methods 0.000 description 7
• 230000035897 transcription Effects 0.000 description 7
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 6
• 101710154606 Hemagglutinin Proteins 0.000 description 6
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 6
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 6
• 108010058846 Ovalbumin Proteins 0.000 description 6
• 239000004365 Protease Substances 0.000 description 6
• 101710176177 Protein A56 Proteins 0.000 description 6
• 241000714474 Rous sarcoma virus Species 0.000 description 6
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 6
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 6
• 229940037003 alum Drugs 0.000 description 6
• 239000003795 chemical substances by application Substances 0.000 description 6
• 238000002474 experimental method Methods 0.000 description 6
• 239000000185 hemagglutinin Substances 0.000 description 6
• IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 6
• 238000001727 in vivo Methods 0.000 description 6
• 230000000670 limiting effect Effects 0.000 description 6
• 238000010839 reverse transcription Methods 0.000 description 6
• 210000000952 spleen Anatomy 0.000 description 6
• PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 6
• 239000003981 vehicle Substances 0.000 description 6
• 241000255925 Diptera Species 0.000 description 5
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 5
• 241000282412 Homo Species 0.000 description 5
• 108091008036 Immune checkpoint proteins Proteins 0.000 description 5
• 102000037982 Immune checkpoint proteins Human genes 0.000 description 5
• 229910019142 PO4 Inorganic materials 0.000 description 5
• 239000004098 Tetracycline Substances 0.000 description 5
• 108700019146 Transgenes Proteins 0.000 description 5
• 108090000631 Trypsin Proteins 0.000 description 5
• 102000004142 Trypsin Human genes 0.000 description 5
• 230000002378 acidificating effect Effects 0.000 description 5
• 230000008901 benefit Effects 0.000 description 5
• 201000011510 cancer Diseases 0.000 description 5
• 230000001413 cellular effect Effects 0.000 description 5
• 210000000349 chromosome Anatomy 0.000 description 5
• 230000000295 complement effect Effects 0.000 description 5
• 239000002299 complementary DNA Substances 0.000 description 5
• 230000001419 dependent effect Effects 0.000 description 5
• 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 5
• 230000002458 infectious effect Effects 0.000 description 5
• 229940092253 ovalbumin Drugs 0.000 description 5
• 230000035515 penetration Effects 0.000 description 5
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 5
• 239000010452 phosphate Substances 0.000 description 5
• 239000002243 precursor Substances 0.000 description 5
• 230000008569 process Effects 0.000 description 5
• 230000028327 secretion Effects 0.000 description 5
• 230000004936 stimulating effect Effects 0.000 description 5
• 239000000126 substance Substances 0.000 description 5
• 230000004083 survival effect Effects 0.000 description 5
• 229960002180 tetracycline Drugs 0.000 description 5
• 229930101283 tetracycline Natural products 0.000 description 5
• 235000019364 tetracycline Nutrition 0.000 description 5
• 150000003522 tetracyclines Chemical class 0.000 description 5
• 238000003146 transient transfection Methods 0.000 description 5
• 239000012588 trypsin Substances 0.000 description 5
• 210000004881 tumor cell Anatomy 0.000 description 5
• 108020005544 Antisense RNA Proteins 0.000 description 4
• 239000004475 Arginine Substances 0.000 description 4
• 102100021992 CD209 antigen Human genes 0.000 description 4
• 102000004190 Enzymes Human genes 0.000 description 4
• 108090000790 Enzymes Proteins 0.000 description 4
• 101000897416 Homo sapiens CD209 antigen Proteins 0.000 description 4
• 108010002350 Interleukin-2 Proteins 0.000 description 4
• 101100519207 Mus musculus Pdcd1 gene Proteins 0.000 description 4
• 102100037935 Polyubiquitin-C Human genes 0.000 description 4
• 230000005867 T cell response Effects 0.000 description 4
• 108010056354 Ubiquitin C Proteins 0.000 description 4
• 208000036142 Viral infection Diseases 0.000 description 4
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
• 239000003184 complementary RNA Substances 0.000 description 4
• 239000003814 drug Substances 0.000 description 4
• 229940066758 endopeptidases Drugs 0.000 description 4
• 229940088598 enzyme Drugs 0.000 description 4
• 238000009472 formulation Methods 0.000 description 4
• 238000001415 gene therapy Methods 0.000 description 4
• 239000002955 immunomodulating agent Substances 0.000 description 4
• 229940121354 immunomodulator Drugs 0.000 description 4
• 230000002584 immunomodulator Effects 0.000 description 4
• 230000003308 immunostimulating effect Effects 0.000 description 4
• 230000001976 improved effect Effects 0.000 description 4
• 230000001965 increasing effect Effects 0.000 description 4
• 238000001802 infusion Methods 0.000 description 4
• 230000002401 inhibitory effect Effects 0.000 description 4
• 238000001990 intravenous administration Methods 0.000 description 4
• 239000003550 marker Substances 0.000 description 4
• 230000001404 mediated effect Effects 0.000 description 4
• 239000002773 nucleotide Substances 0.000 description 4
• 125000003729 nucleotide group Chemical group 0.000 description 4
• 230000035939 shock Effects 0.000 description 4
• 239000000243 solution Substances 0.000 description 4
• 241000894007 species Species 0.000 description 4
• 101800001643 6K protein Proteins 0.000 description 3
• 208000030507 AIDS Diseases 0.000 description 3
• 241000238876 Acari Species 0.000 description 3
• 241000271566 Aves Species 0.000 description 3
• 108010029697 CD40 Ligand Proteins 0.000 description 3
• 102100032937 CD40 ligand Human genes 0.000 description 3
• 241000725619 Dengue virus Species 0.000 description 3
• 206010059866 Drug resistance Diseases 0.000 description 3
• 241000283086 Equidae Species 0.000 description 3
• 241000282326 Felis catus Species 0.000 description 3
• 241000287828 Gallus gallus Species 0.000 description 3
• 239000012981 Hank's balanced salt solution Substances 0.000 description 3
• 108010034145 Helminth Proteins Proteins 0.000 description 3
• 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 3
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 3
• 108700018351 Major Histocompatibility Complex Proteins 0.000 description 3
• 101710127721 Membrane protein Proteins 0.000 description 3
• 235000021314 Palmitic acid Nutrition 0.000 description 3
• 102000035195 Peptidases Human genes 0.000 description 3
• 108091005804 Peptidases Proteins 0.000 description 3
• NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 3
• 108010076504 Protein Sorting Signals Proteins 0.000 description 3
• 101710185720 Putative ethidium bromide resistance protein Proteins 0.000 description 3
• 108700008625 Reporter Genes Proteins 0.000 description 3
• 108091028664 Ribonucleotide Proteins 0.000 description 3
• 102000003667 Serine Endopeptidases Human genes 0.000 description 3
• 108090000083 Serine Endopeptidases Proteins 0.000 description 3
• 241000272534 Struthio camelus Species 0.000 description 3
• 241000710959 Venezuelan equine encephalitis virus Species 0.000 description 3
• 108010067390 Viral Proteins Proteins 0.000 description 3
• 210000000612 antigen-presenting cell Anatomy 0.000 description 3
• 229940009098 aspartate Drugs 0.000 description 3
• 210000003719 b-lymphocyte Anatomy 0.000 description 3
• 210000001185 bone marrow Anatomy 0.000 description 3
• 239000000872 buffer Substances 0.000 description 3
• 239000001506 calcium phosphate Substances 0.000 description 3
• 229910000389 calcium phosphate Inorganic materials 0.000 description 3
• 235000011010 calcium phosphates Nutrition 0.000 description 3
• 210000000234 capsid Anatomy 0.000 description 3
• 238000004113 cell culture Methods 0.000 description 3
• 239000006143 cell culture medium Substances 0.000 description 3
• 210000000170 cell membrane Anatomy 0.000 description 3
• 210000002421 cell wall Anatomy 0.000 description 3
• 235000013330 chicken meat Nutrition 0.000 description 3
• 210000005220 cytoplasmic tail Anatomy 0.000 description 3
• 238000001514 detection method Methods 0.000 description 3
• 238000010586 diagram Methods 0.000 description 3
• 238000010790 dilution Methods 0.000 description 3
• 239000012895 dilution Substances 0.000 description 3
• 229940079593 drug Drugs 0.000 description 3
• 239000003937 drug carrier Substances 0.000 description 3
• 239000000839 emulsion Substances 0.000 description 3
• 210000003527 eukaryotic cell Anatomy 0.000 description 3
• 244000000013 helminth Species 0.000 description 3
• 230000002163 immunogen Effects 0.000 description 3
• 229960001438 immunostimulant agent Drugs 0.000 description 3
• 239000003022 immunostimulating agent Substances 0.000 description 3
• 239000012212 insulator Substances 0.000 description 3
• 230000003993 interaction Effects 0.000 description 3
• 239000003446 ligand Substances 0.000 description 3
• 150000002632 lipids Chemical class 0.000 description 3
• 239000007788 liquid Substances 0.000 description 3
• 210000001165 lymph node Anatomy 0.000 description 3
• 230000004048 modification Effects 0.000 description 3
• 238000012986 modification Methods 0.000 description 3
• WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 3
• 229920000642 polymer Polymers 0.000 description 3
• 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
• 229920000053 polysorbate 80 Polymers 0.000 description 3
• 230000001737 promoting effect Effects 0.000 description 3
• 230000000069 prophylactic effect Effects 0.000 description 3
• 230000004044 response Effects 0.000 description 3
• 239000002336 ribonucleotide Substances 0.000 description 3
• 125000002652 ribonucleotide group Chemical group 0.000 description 3
• 150000007949 saponins Chemical class 0.000 description 3
• 238000002864 sequence alignment Methods 0.000 description 3
• 238000010561 standard procedure Methods 0.000 description 3
• 150000003431 steroids Chemical class 0.000 description 3
• 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 3
• 230000010474 transient expression Effects 0.000 description 3
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
• 241000701161 unidentified adenovirus Species 0.000 description 3
• 230000009385 viral infection Effects 0.000 description 3
• 210000002845 virion Anatomy 0.000 description 3
• MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 2
• YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 2
• NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
• DLZKEQQWXODGGZ-KCJUWKMLSA-N 2-[[(2r)-2-[[(2s)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]propanoyl]amino]acetic acid Chemical compound OC(=O)CNC(=O)[C@@H](C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 DLZKEQQWXODGGZ-KCJUWKMLSA-N 0.000 description 2
• 102100023635 Alpha-fetoprotein Human genes 0.000 description 2
• NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
• 102000006734 Beta-Globulins Human genes 0.000 description 2
• 108010087504 Beta-Globulins Proteins 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• 206010006187 Breast cancer Diseases 0.000 description 2
• 208000026310 Breast neoplasm Diseases 0.000 description 2
• 108010004032 Bromelains Proteins 0.000 description 2
• 101150013553 CD40 gene Proteins 0.000 description 2
• 108010032088 Calpain Proteins 0.000 description 2
• 102000007590 Calpain Human genes 0.000 description 2
• 241000282472 Canis lupus familiaris Species 0.000 description 2
• 102100024423 Carbonic anhydrase 9 Human genes 0.000 description 2
• 102000003908 Cathepsin D Human genes 0.000 description 2
• 108090000258 Cathepsin D Proteins 0.000 description 2
• 102000019034 Chemokines Human genes 0.000 description 2
• 108010012236 Chemokines Proteins 0.000 description 2
• 108090000746 Chymosin Proteins 0.000 description 2
• 108090000317 Chymotrypsin Proteins 0.000 description 2
• 102000029816 Collagenase Human genes 0.000 description 2
• 108060005980 Collagenase Proteins 0.000 description 2
• 206010009944 Colon cancer Diseases 0.000 description 2
• 208000035473 Communicable disease Diseases 0.000 description 2
• 108090000395 Cysteine Endopeptidases Proteins 0.000 description 2
• 102000003950 Cysteine Endopeptidases Human genes 0.000 description 2
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
• 101710201734 E3 protein Proteins 0.000 description 2
• 241000214054 Equine rhinitis A virus Species 0.000 description 2
• 108010048049 Factor IXa Proteins 0.000 description 2
• 108010014173 Factor X Proteins 0.000 description 2
• 241000710831 Flavivirus Species 0.000 description 2
• 241000710198 Foot-and-mouth disease virus Species 0.000 description 2
• 108090000079 Glucocorticoid Receptors Proteins 0.000 description 2
• 102100033417 Glucocorticoid receptor Human genes 0.000 description 2
• 102100041003 Glutamate carboxypeptidase 2 Human genes 0.000 description 2
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 2
• 108010010369 HIV Protease Proteins 0.000 description 2
• HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
• 101000892862 Homo sapiens Glutamate carboxypeptidase 2 Proteins 0.000 description 2
• 208000031300 Hydrocephalus with stenosis of the aqueduct of Sylvius Diseases 0.000 description 2
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
• 206010021143 Hypoxia Diseases 0.000 description 2
• 206010061598 Immunodeficiency Diseases 0.000 description 2
• 208000029462 Immunodeficiency disease Diseases 0.000 description 2
• 102100022297 Integrin alpha-X Human genes 0.000 description 2
• 239000000232 Lipid Bilayer Substances 0.000 description 2
• 208000016604 Lyme disease Diseases 0.000 description 2
• 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
• 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 2
• 102100028389 Melanoma antigen recognized by T-cells 1 Human genes 0.000 description 2
• 108090000131 Metalloendopeptidases Proteins 0.000 description 2
• 102000003843 Metalloendopeptidases Human genes 0.000 description 2
• 108090000157 Metallothionein Proteins 0.000 description 2
• 241000204031 Mycoplasma Species 0.000 description 2
• 108700020354 N-acetylmuramyl-threonyl-isoglutamine Proteins 0.000 description 2
• 102000005348 Neuraminidase Human genes 0.000 description 2
• 108010006232 Neuraminidase Proteins 0.000 description 2
• PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
• 108091028043 Nucleic acid sequence Proteins 0.000 description 2
• 108090000526 Papain Proteins 0.000 description 2
• 101800001821 Precursor of protein E3/E2 Proteins 0.000 description 2
• RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
• 102000007066 Prostate-Specific Antigen Human genes 0.000 description 2
• 108010072866 Prostate-Specific Antigen Proteins 0.000 description 2
• 241000508269 Psidium Species 0.000 description 2
• 241000711798 Rabies lyssavirus Species 0.000 description 2
• 208000006265 Renal cell carcinoma Diseases 0.000 description 2
• 108091027981 Response element Proteins 0.000 description 2
• 241000242678 Schistosoma Species 0.000 description 2
• 102000012479 Serine Proteases Human genes 0.000 description 2
• 108010022999 Serine Proteases Proteins 0.000 description 2
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
• 108010023197 Streptokinase Proteins 0.000 description 2
• 101800001271 Surface protein Proteins 0.000 description 2
• 108700026226 TATA Box Proteins 0.000 description 2
• BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 2
• 108090001109 Thermolysin Proteins 0.000 description 2
• 108090000190 Thrombin Proteins 0.000 description 2
• 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 2
• 102000003425 Tyrosinase Human genes 0.000 description 2
• 108060008724 Tyrosinase Proteins 0.000 description 2
• 102000044159 Ubiquitin Human genes 0.000 description 2
• 108090000848 Ubiquitin Proteins 0.000 description 2
• 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 2
• 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 2
• 108020005202 Viral DNA Proteins 0.000 description 2
• 241001492404 Woodchuck hepatitis virus Species 0.000 description 2
• 208000026197 X-linked hydrocephalus with stenosis of the aqueduct of Sylvius Diseases 0.000 description 2
• 239000002253 acid Substances 0.000 description 2
• 125000002015 acyclic group Chemical group 0.000 description 2
• 230000002411 adverse Effects 0.000 description 2
• 125000003342 alkenyl group Chemical group 0.000 description 2
• 125000000304 alkynyl group Chemical group 0.000 description 2
• NWMHDZMRVUOQGL-CZEIJOLGSA-N almurtide Chemical compound OC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)CO[C@@H]([C@H](O)[C@H](O)CO)[C@@H](NC(C)=O)C=O NWMHDZMRVUOQGL-CZEIJOLGSA-N 0.000 description 2
• 108010026331 alpha-Fetoproteins Proteins 0.000 description 2
• 230000000890 antigenic effect Effects 0.000 description 2
• 239000013011 aqueous formulation Substances 0.000 description 2
• 210000002798 bone marrow cell Anatomy 0.000 description 2
• 235000019835 bromelain Nutrition 0.000 description 2
• 210000004899 c-terminal region Anatomy 0.000 description 2
• 229940080701 chymosin Drugs 0.000 description 2
• 229960002376 chymotrypsin Drugs 0.000 description 2
• 229960002424 collagenase Drugs 0.000 description 2
• 208000029742 colonic neoplasm Diseases 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 238000012258 culturing Methods 0.000 description 2
• 210000000805 cytoplasm Anatomy 0.000 description 2
• 230000001086 cytosolic effect Effects 0.000 description 2
• 230000003247 decreasing effect Effects 0.000 description 2
• 230000004041 dendritic cell maturation Effects 0.000 description 2
• 230000029087 digestion Effects 0.000 description 2
• 239000000975 dye Substances 0.000 description 2
• 230000012202 endocytosis Effects 0.000 description 2
• 230000002255 enzymatic effect Effects 0.000 description 2
• 210000000981 epithelium Anatomy 0.000 description 2
• 102000034287 fluorescent proteins Human genes 0.000 description 2
• 108091006047 fluorescent proteins Proteins 0.000 description 2
• 230000002538 fungal effect Effects 0.000 description 2
• 238000001476 gene delivery Methods 0.000 description 2
• 238000010353 genetic engineering Methods 0.000 description 2
• 108010028403 hemagglutinin esterase Proteins 0.000 description 2
• 229920000669 heparin Polymers 0.000 description 2
• 229960002897 heparin Drugs 0.000 description 2
• 208000002672 hepatitis B Diseases 0.000 description 2
• 230000028996 humoral immune response Effects 0.000 description 2
• 239000001257 hydrogen Substances 0.000 description 2
• 229910052739 hydrogen Inorganic materials 0.000 description 2
• 230000007954 hypoxia Effects 0.000 description 2
• 210000002865 immune cell Anatomy 0.000 description 2
• 230000008105 immune reaction Effects 0.000 description 2
• 230000007813 immunodeficiency Effects 0.000 description 2
• 230000005847 immunogenicity Effects 0.000 description 2
• 238000011065 in-situ storage Methods 0.000 description 2
• 238000010348 incorporation Methods 0.000 description 2
• 239000003112 inhibitor Substances 0.000 description 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
• GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical class O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 2
• 238000011068 loading method Methods 0.000 description 2
• 230000007774 longterm Effects 0.000 description 2
• 210000004698 lymphocyte Anatomy 0.000 description 2
• 210000005210 lymphoid organ Anatomy 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 238000005259 measurement Methods 0.000 description 2
• 201000001441 melanoma Diseases 0.000 description 2
• 229910021645 metal ion Inorganic materials 0.000 description 2
• 229960005225 mifamurtide Drugs 0.000 description 2
• JMUHBNWAORSSBD-WKYWBUFDSA-N mifamurtide Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCCCCCCCCCC)COP(O)(=O)OCCNC(=O)[C@H](C)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1NC(C)=O JMUHBNWAORSSBD-WKYWBUFDSA-N 0.000 description 2
• 238000012544 monitoring process Methods 0.000 description 2
• GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical compound C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 description 2
• JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 2
• 239000007764 o/w emulsion Substances 0.000 description 2
• 210000000496 pancreas Anatomy 0.000 description 2
• 229940055729 papain Drugs 0.000 description 2
• 235000019834 papain Nutrition 0.000 description 2
• 230000008506 pathogenesis Effects 0.000 description 2
• 230000003389 potentiating effect Effects 0.000 description 2
• 239000002244 precipitate Substances 0.000 description 2
• 230000002265 prevention Effects 0.000 description 2
• 230000001566 pro-viral effect Effects 0.000 description 2
• 235000019419 proteases Nutrition 0.000 description 2
• 238000000746 purification Methods 0.000 description 2
• 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
• 230000008707 rearrangement Effects 0.000 description 2
• 238000011160 research Methods 0.000 description 2
• 108091008146 restriction endonucleases Proteins 0.000 description 2
• 238000012552 review Methods 0.000 description 2
• 229930182490 saponin Natural products 0.000 description 2
• FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 2
• 238000000926 separation method Methods 0.000 description 2
• 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 2
• 210000004988 splenocyte Anatomy 0.000 description 2
• 229940032094 squalane Drugs 0.000 description 2
• 229940031439 squalene Drugs 0.000 description 2
• TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 2
• 229960005202 streptokinase Drugs 0.000 description 2
• 238000007920 subcutaneous administration Methods 0.000 description 2
• 230000008685 targeting Effects 0.000 description 2
• 238000002560 therapeutic procedure Methods 0.000 description 2
• 229960004072 thrombin Drugs 0.000 description 2
• 230000002103 transcriptional effect Effects 0.000 description 2
• 238000012546 transfer Methods 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• 125000002889 tridecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
• 230000004614 tumor growth Effects 0.000 description 2
• 125000002948 undecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
• 241001529453 unidentified herpesvirus Species 0.000 description 2
• 230000007502 viral entry Effects 0.000 description 2
• 210000000605 viral structure Anatomy 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
• JVJGCCBAOOWGEO-RUTPOYCXSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-4-amino-2-[[(2s,3s)-2-[[(2s,3s)-2-[[(2s)-2-azaniumyl-3-hydroxypropanoyl]amino]-3-methylpentanoyl]amino]-3-methylpentanoyl]amino]-4-oxobutanoyl]amino]-3-phenylpropanoyl]amino]-4-carboxylatobutanoyl]amino]-6-azaniumy Chemical compound OC[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O)CC1=CC=CC=C1 JVJGCCBAOOWGEO-RUTPOYCXSA-N 0.000 description 1
• YHQZWWDVLJPRIF-JLHRHDQISA-N (4R)-4-[[(2S,3R)-2-[acetyl-[(3R,4R,5S,6R)-3-amino-4-[(1R)-1-carboxyethoxy]-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]amino]-3-hydroxybutanoyl]amino]-5-amino-5-oxopentanoic acid Chemical compound C(C)(=O)N([C@@H]([C@H](O)C)C(=O)N[C@H](CCC(=O)O)C(N)=O)C1[C@H](N)[C@@H](O[C@@H](C(=O)O)C)[C@H](O)[C@H](O1)CO YHQZWWDVLJPRIF-JLHRHDQISA-N 0.000 description 1
• WHTVZRBIWZFKQO-AWEZNQCLSA-N (S)-chloroquine Chemical compound ClC1=CC=C2C(N[C@@H](C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-AWEZNQCLSA-N 0.000 description 1
• 125000004973 1-butenyl group Chemical group C(=CCC)* 0.000 description 1
• 125000004972 1-butynyl group Chemical group [H]C([H])([H])C([H])([H])C#C* 0.000 description 1
• 125000006023 1-pentenyl group Chemical group 0.000 description 1
• OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
• 125000006069 2,3-dimethyl-2-butenyl group Chemical group 0.000 description 1
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
• GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
• 125000004974 2-butenyl group Chemical group C(C=CC)* 0.000 description 1
• 125000000069 2-butynyl group Chemical group [H]C([H])([H])C#CC([H])([H])* 0.000 description 1
• 125000006029 2-methyl-2-butenyl group Chemical group 0.000 description 1
• 125000006024 2-pentenyl group Chemical group 0.000 description 1
• 125000006027 3-methyl-1-butenyl group Chemical group 0.000 description 1
• 241000235389 Absidia Species 0.000 description 1
• 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
• 241000186046 Actinomyces Species 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 102100027211 Albumin Human genes 0.000 description 1
• 108010088751 Albumins Proteins 0.000 description 1
• 241001136782 Alca Species 0.000 description 1
• 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
• 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
• 102100037982 Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Human genes 0.000 description 1
• 241000223600 Alternaria Species 0.000 description 1
• 101710145634 Antigen 1 Proteins 0.000 description 1
• 241000272478 Aquila Species 0.000 description 1
• 241000219194 Arabidopsis Species 0.000 description 1
• 241000239290 Araneae Species 0.000 description 1
• 241000238421 Arthropoda Species 0.000 description 1
• 241000244186 Ascaris Species 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 241000228212 Aspergillus Species 0.000 description 1
• 208000023275 Autoimmune disease Diseases 0.000 description 1
• 241000713842 Avian sarcoma virus Species 0.000 description 1
• 102100035526 B melanoma antigen 1 Human genes 0.000 description 1
• 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 1
• 102000008096 B7-H1 Antigen Human genes 0.000 description 1
• 108010074708 B7-H1 Antigen Proteins 0.000 description 1
• 241000223836 Babesia Species 0.000 description 1
• 241000193830 Bacillus <bacterium> Species 0.000 description 1
• 241000894006 Bacteria Species 0.000 description 1
• 208000035143 Bacterial infection Diseases 0.000 description 1
• 241000606125 Bacteroides Species 0.000 description 1
• 102100021663 Baculoviral IAP repeat-containing protein 5 Human genes 0.000 description 1
• 241000606660 Bartonella Species 0.000 description 1
• 241001465178 Bipolaris Species 0.000 description 1
• 241000588807 Bordetella Species 0.000 description 1
• 241000589968 Borrelia Species 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 241000701822 Bovine papillomavirus Species 0.000 description 1
• 241000589562 Brucella Species 0.000 description 1
• 241000244036 Brugia Species 0.000 description 1
• 102100040840 C-type lectin domain family 7 member A Human genes 0.000 description 1
• 102000002086 C-type lectin-like Human genes 0.000 description 1
• 108050009406 C-type lectin-like Proteins 0.000 description 1
• 108700012439 CA9 Proteins 0.000 description 1
• 102100032912 CD44 antigen Human genes 0.000 description 1
• 101100314454 Caenorhabditis elegans tra-1 gene Proteins 0.000 description 1
• 241000589876 Campylobacter Species 0.000 description 1
• 102100025570 Cancer/testis antigen 1 Human genes 0.000 description 1
• 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
• 241000253350 Capillaria Species 0.000 description 1
• 241000190890 Capnocytophaga Species 0.000 description 1
• 108090000565 Capsid Proteins Proteins 0.000 description 1
• 101710132601 Capsid protein Proteins 0.000 description 1
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
• 239000004215 Carbon black (E152) Substances 0.000 description 1
• 108010033547 Carbonic Anhydrase I Proteins 0.000 description 1
• 102100025518 Carbonic anhydrase 1 Human genes 0.000 description 1
• 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
• 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 1
• 241001428800 Cell fusing agent virus Species 0.000 description 1
• 102100023321 Ceruloplasmin Human genes 0.000 description 1
• 241000606161 Chlamydia Species 0.000 description 1
• 108091062157 Cis-regulatory element Proteins 0.000 description 1
• 241000193403 Clostridium Species 0.000 description 1
• 108020004705 Codon Proteins 0.000 description 1
• 241001480517 Conidiobolus Species 0.000 description 1
• 241000711573 Coronaviridae Species 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 241001445332 Coxiella <snail> Species 0.000 description 1
• 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
• 241000223935 Cryptosporidium Species 0.000 description 1
• 102000012466 Cytochrome P450 1B1 Human genes 0.000 description 1
• 108050002014 Cytochrome P450 1B1 Proteins 0.000 description 1
• 102100039498 Cytotoxic T-lymphocyte protein 4 Human genes 0.000 description 1
• YVGGHNCTFXOJCH-UHFFFAOYSA-N DDT Chemical compound C1=CC(Cl)=CC=C1C(C(Cl)(Cl)Cl)C1=CC=C(Cl)C=C1 YVGGHNCTFXOJCH-UHFFFAOYSA-N 0.000 description 1
• 102000053602 DNA Human genes 0.000 description 1
• 238000001712 DNA sequencing Methods 0.000 description 1
• 230000006820 DNA synthesis Effects 0.000 description 1
• 230000004568 DNA-binding Effects 0.000 description 1
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
• 108090000204 Dipeptidase 1 Proteins 0.000 description 1
• 241000189163 Dipetalonema Species 0.000 description 1
• 241000243990 Dirofilaria Species 0.000 description 1
• 208000000655 Distemper Diseases 0.000 description 1
• SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
• 238000008157 ELISA kit Methods 0.000 description 1
• 101150029707 ERBB2 gene Proteins 0.000 description 1
• 241001115402 Ebolavirus Species 0.000 description 1
• 241000605314 Ehrlichia Species 0.000 description 1
• 241000223924 Eimeria Species 0.000 description 1
• 241000498256 Enterobius Species 0.000 description 1
• 241000194033 Enterococcus Species 0.000 description 1
• 241000709661 Enterovirus Species 0.000 description 1
• 241000991587 Enterovirus C Species 0.000 description 1
• 101710189136 Envelope fusion protein Proteins 0.000 description 1
• 101800001467 Envelope glycoprotein E2 Proteins 0.000 description 1
• 101710204837 Envelope small membrane protein Proteins 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• 241000713730 Equine infectious anemia virus Species 0.000 description 1
• 241000588722 Escherichia Species 0.000 description 1
• 241000588724 Escherichia coli Species 0.000 description 1
• 102100038595 Estrogen receptor Human genes 0.000 description 1
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
• XZWYTXMRWQJBGX-VXBMVYAYSA-N FLAG peptide Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=C(O)C=C1 XZWYTXMRWQJBGX-VXBMVYAYSA-N 0.000 description 1
• 108010074860 Factor Xa Proteins 0.000 description 1
• 241000986243 Filaroides Species 0.000 description 1
• 241000710781 Flaviviridae Species 0.000 description 1
• 241000700662 Fowlpox virus Species 0.000 description 1
• 241000589601 Francisella Species 0.000 description 1
• 206010017533 Fungal infection Diseases 0.000 description 1
• 241000233866 Fungi Species 0.000 description 1
• 241001183197 Fusobacteriales Species 0.000 description 1
• 241000224466 Giardia Species 0.000 description 1
• 241000257324 Glossina <genus> Species 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 108010070675 Glutathione transferase Proteins 0.000 description 1
• 102000005720 Glutathione transferase Human genes 0.000 description 1
• 244000068988 Glycine max Species 0.000 description 1
• 235000010469 Glycine max Nutrition 0.000 description 1
• 229920002683 Glycosaminoglycan Polymers 0.000 description 1
• 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
• 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 1
• 239000007995 HEPES buffer Substances 0.000 description 1
• 208000031886 HIV Infections Diseases 0.000 description 1
• 241000606790 Haemophilus Species 0.000 description 1
• 241000606768 Haemophilus influenzae Species 0.000 description 1
• 241000406101 Hammondia Species 0.000 description 1
• 241000589989 Helicobacter Species 0.000 description 1
• 241000700721 Hepatitis B virus Species 0.000 description 1
• 108700008783 Hepatitis C virus E1 Proteins 0.000 description 1
• 108010073141 Hepatitis C virus glycoprotein E2 Proteins 0.000 description 1
• 208000007514 Herpes zoster Diseases 0.000 description 1
• 241000238631 Hexapoda Species 0.000 description 1
• 241000228402 Histoplasma Species 0.000 description 1
• 108010025076 Holoenzymes Proteins 0.000 description 1
• 102100028092 Homeobox protein Nkx-3.1 Human genes 0.000 description 1
• 101000874316 Homo sapiens B melanoma antigen 1 Proteins 0.000 description 1
• 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 1
• 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 1
• 101000856237 Homo sapiens Cancer/testis antigen 1 Proteins 0.000 description 1
• 101000980898 Homo sapiens Cell division cycle-associated protein 4 Proteins 0.000 description 1
• 101000889276 Homo sapiens Cytotoxic T-lymphocyte protein 4 Proteins 0.000 description 1
• 101000882584 Homo sapiens Estrogen receptor Proteins 0.000 description 1
• 101000578249 Homo sapiens Homeobox protein Nkx-3.1 Proteins 0.000 description 1
• 101001046870 Homo sapiens Hypoxia-inducible factor 1-alpha Proteins 0.000 description 1
• 101001046687 Homo sapiens Integrin alpha-E Proteins 0.000 description 1
• 101000578784 Homo sapiens Melanoma antigen recognized by T-cells 1 Proteins 0.000 description 1
• 101000628547 Homo sapiens Metalloreductase STEAP1 Proteins 0.000 description 1
• 101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 description 1
• 101000763314 Homo sapiens Thrombomodulin Proteins 0.000 description 1
• 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
• 241000713340 Human immunodeficiency virus 2 Species 0.000 description 1
• 241000701806 Human papillomavirus Species 0.000 description 1
• 208000022361 Human papillomavirus infectious disease Diseases 0.000 description 1
• 241000257303 Hymenoptera Species 0.000 description 1
• 102100022875 Hypoxia-inducible factor 1-alpha Human genes 0.000 description 1
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
• 108060003951 Immunoglobulin Proteins 0.000 description 1
• 108700005091 Immunoglobulin Genes Proteins 0.000 description 1
• 108090001061 Insulin Proteins 0.000 description 1
• 102100023915 Insulin Human genes 0.000 description 1
• 102000012330 Integrases Human genes 0.000 description 1
• 108010050904 Interferons Proteins 0.000 description 1
• 102000014150 Interferons Human genes 0.000 description 1
• 108010002352 Interleukin-1 Proteins 0.000 description 1
• 102000000589 Interleukin-1 Human genes 0.000 description 1
• 108090000172 Interleukin-15 Proteins 0.000 description 1
• 108010065637 Interleukin-23 Proteins 0.000 description 1
• 108090000978 Interleukin-4 Proteins 0.000 description 1
• 108090001005 Interleukin-6 Proteins 0.000 description 1
• 108010002586 Interleukin-7 Proteins 0.000 description 1
• 102000015696 Interleukins Human genes 0.000 description 1
• 108010063738 Interleukins Proteins 0.000 description 1
• 241000567229 Isospora Species 0.000 description 1
• 241000588748 Klebsiella Species 0.000 description 1
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
• 241000222722 Leishmania <genus> Species 0.000 description 1
• 241001131651 Leptosomus discolor Species 0.000 description 1
• 241000589902 Leptospira Species 0.000 description 1
• 108010028921 Lipopeptides Proteins 0.000 description 1
• 241000186781 Listeria Species 0.000 description 1
• 108060001084 Luciferase Proteins 0.000 description 1
• 239000005089 Luciferase Substances 0.000 description 1
• 101710157884 Lymphocyte antigen 75 Proteins 0.000 description 1
• 102100033486 Lymphocyte antigen 75 Human genes 0.000 description 1
• 206010025323 Lymphomas Diseases 0.000 description 1
• 101710145006 Lysis protein Proteins 0.000 description 1
• 108010010995 MART-1 Antigen Proteins 0.000 description 1
• 102000043131 MHC class II family Human genes 0.000 description 1
• 108091054438 MHC class II family Proteins 0.000 description 1
• GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• 108010031099 Mannose Receptor Proteins 0.000 description 1
• 241000142892 Mansonella Species 0.000 description 1
• 241001115401 Marburgvirus Species 0.000 description 1
• 101710085938 Matrix protein Proteins 0.000 description 1
• 102000018697 Membrane Proteins Human genes 0.000 description 1
• 108010052285 Membrane Proteins Proteins 0.000 description 1
• 102000003735 Mesothelin Human genes 0.000 description 1
• 108090000015 Mesothelin Proteins 0.000 description 1
• 102100026712 Metalloreductase STEAP1 Human genes 0.000 description 1
• 102000003792 Metallothionein Human genes 0.000 description 1
• 241000243190 Microsporidia Species 0.000 description 1
• 241000542980 Mimidae Species 0.000 description 1
• 241001092142 Molina Species 0.000 description 1
• 229930191564 Monensin Natural products 0.000 description 1
• GAOZTHIDHYLHMS-UHFFFAOYSA-N Monensin A Natural products O1C(CC)(C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)CCC1C(O1)(C)CCC21CC(O)C(C)C(C(C)C(OC)C(C)C(O)=O)O2 GAOZTHIDHYLHMS-UHFFFAOYSA-N 0.000 description 1
• 241000908267 Moniliella Species 0.000 description 1
• 241000235575 Mortierella Species 0.000 description 1
• 241000713333 Mouse mammary tumor virus Species 0.000 description 1
• 241000699666 Mus <mouse, genus> Species 0.000 description 1
• 208000031888 Mycoses Diseases 0.000 description 1
• 108700015872 N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine Proteins 0.000 description 1
• 230000004988 N-glycosylation Effects 0.000 description 1
• 108010057466 NF-kappa B Proteins 0.000 description 1
• 102000003945 NF-kappa B Human genes 0.000 description 1
• 241000588653 Neisseria Species 0.000 description 1
• 241000244206 Nematoda Species 0.000 description 1
• 241000216206 Neogobius Species 0.000 description 1
• 229930193140 Neomycin Natural products 0.000 description 1
• 108700019961 Neoplasm Genes Proteins 0.000 description 1
• 102000048850 Neoplasm Genes Human genes 0.000 description 1
• 241001468109 Neorickettsia Species 0.000 description 1
• 241001147660 Neospora Species 0.000 description 1
• 241000187654 Nocardia Species 0.000 description 1
• 241001126829 Nosema Species 0.000 description 1
• 101710163270 Nuclease Proteins 0.000 description 1
• 102000011931 Nucleoproteins Human genes 0.000 description 1
• 108010061100 Nucleoproteins Proteins 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 241000243981 Onchocerca Species 0.000 description 1
• 108700020796 Oncogene Proteins 0.000 description 1
• 241000283973 Oryctolagus cuniculus Species 0.000 description 1
• 108700006640 OspA Proteins 0.000 description 1
• 241000243795 Ostertagia Species 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 108010067372 Pancreatic elastase Proteins 0.000 description 1
• 102000016387 Pancreatic elastase Human genes 0.000 description 1
• 241001631646 Papillomaviridae Species 0.000 description 1
• 229930040373 Paraformaldehyde Natural products 0.000 description 1
• 241001480233 Paragonimus Species 0.000 description 1
• 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
• 208000030852 Parasitic disease Diseases 0.000 description 1
• 241000606860 Pasteurella Species 0.000 description 1
• 235000019483 Peanut oil Nutrition 0.000 description 1
• 241001494479 Pecora Species 0.000 description 1
• 241000228143 Penicillium Species 0.000 description 1
• 241000206591 Peptococcus Species 0.000 description 1
• 241000191992 Peptostreptococcus Species 0.000 description 1
• 208000037581 Persistent Infection Diseases 0.000 description 1
• 241000710778 Pestivirus Species 0.000 description 1
• 241000255129 Phlebotominae Species 0.000 description 1
• 241001674048 Phthiraptera Species 0.000 description 1
• 241000709664 Picornaviridae Species 0.000 description 1
• 206010035226 Plasma cell myeloma Diseases 0.000 description 1
• 241000224016 Plasmodium Species 0.000 description 1
• 241000233870 Pneumocystis Species 0.000 description 1
• 241000281047 Polioptila plumbea Species 0.000 description 1
• 108010020346 Polyglutamic Acid Proteins 0.000 description 1
• 108010039918 Polylysine Proteins 0.000 description 1
• 241001505332 Polyomavirus sp. Species 0.000 description 1
• ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
• 102100025803 Progesterone receptor Human genes 0.000 description 1
• 206010060862 Prostate cancer Diseases 0.000 description 1
• 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
• 241000588769 Proteus <enterobacteria> Species 0.000 description 1
• 241000125945 Protoparvovirus Species 0.000 description 1
• 241000196250 Prototheca Species 0.000 description 1
• 241000589516 Pseudomonas Species 0.000 description 1
• 235000009001 Quillaja saponaria Nutrition 0.000 description 1
• 241001454523 Quillaja saponaria Species 0.000 description 1
• 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
• 230000004570 RNA-binding Effects 0.000 description 1
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
• 101001023863 Rattus norvegicus Glucocorticoid receptor Proteins 0.000 description 1
• 241000702263 Reovirus sp. Species 0.000 description 1
• 108010073443 Ribi adjuvant Proteins 0.000 description 1
• 241000606701 Rickettsia Species 0.000 description 1
• 241000702670 Rotavirus Species 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 108010077895 Sarcosine Proteins 0.000 description 1
• 241000710961 Semliki Forest virus Species 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 235000005775 Setaria Nutrition 0.000 description 1
• 241000232088 Setaria <nematode> Species 0.000 description 1
• 241000607768 Shigella Species 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• 241000256103 Simuliidae Species 0.000 description 1
• 108020004459 Small interfering RNA Proteins 0.000 description 1
• PRXRUNOAOLTIEF-ADSICKODSA-N Sorbitan trioleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCC\C=C/CCCCCCCC)[C@H]1OC[C@H](O)[C@H]1OC(=O)CCCCCCC\C=C/CCCCCCCC PRXRUNOAOLTIEF-ADSICKODSA-N 0.000 description 1
• 241000203992 Spirometra Species 0.000 description 1
• 241000191940 Staphylococcus Species 0.000 description 1
• 241000194017 Streptococcus Species 0.000 description 1
• 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
• 241000193996 Streptococcus pyogenes Species 0.000 description 1
• 241000244174 Strongyloides Species 0.000 description 1
• 101710172711 Structural protein Proteins 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• 208000002847 Surgical Wound Diseases 0.000 description 1
• 108010002687 Survivin Proteins 0.000 description 1
• 230000024932 T cell mediated immunity Effects 0.000 description 1
• 230000037453 T cell priming Effects 0.000 description 1
• 241001365914 Taira Species 0.000 description 1
• 108010017842 Telomerase Proteins 0.000 description 1
• 102100036407 Thioredoxin Human genes 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• 102100026966 Thrombomodulin Human genes 0.000 description 1
• 108020000411 Toll-like receptor Proteins 0.000 description 1
• 241000607216 Toxascaris Species 0.000 description 1
• 101710120037 Toxin CcdB Proteins 0.000 description 1
• 241000223996 Toxoplasma Species 0.000 description 1
• 108090000333 Transgelin Proteins 0.000 description 1
• 102000003932 Transgelin Human genes 0.000 description 1
• 102100023935 Transmembrane glycoprotein NMB Human genes 0.000 description 1
• 241000589886 Treponema Species 0.000 description 1
• 241000243774 Trichinella Species 0.000 description 1
• 241000893966 Trichophyton verrucosum Species 0.000 description 1
• 241000223104 Trypanosoma Species 0.000 description 1
• 208000025865 Ulcer Diseases 0.000 description 1
• 241000571986 Uncinaria Species 0.000 description 1
• 241000700618 Vaccinia virus Species 0.000 description 1
• 108700005077 Viral Genes Proteins 0.000 description 1
• 108010052104 Viral Regulatory and Accessory Proteins Proteins 0.000 description 1
• 108010066342 Virus Receptors Proteins 0.000 description 1
• 102000018265 Virus Receptors Human genes 0.000 description 1
• 241000713325 Visna/maedi virus Species 0.000 description 1
• 108091093126 WHP Posttrascriptional Response Element Proteins 0.000 description 1
• 241000710886 West Nile virus Species 0.000 description 1
• 241000244005 Wuchereria bancrofti Species 0.000 description 1
• 241000710772 Yellow fever virus Species 0.000 description 1
• 240000008042 Zea mays Species 0.000 description 1
• 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
• 230000004913 activation Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000004480 active ingredient Substances 0.000 description 1
• 230000006978 adaptation Effects 0.000 description 1
• 230000033289 adaptive immune response Effects 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 238000012382 advanced drug delivery Methods 0.000 description 1
• 239000013566 allergen Substances 0.000 description 1
• 108010034034 alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase Proteins 0.000 description 1
• 230000004075 alteration Effects 0.000 description 1
• AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
• ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
• 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
• DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
• 235000019270 ammonium chloride Nutrition 0.000 description 1
• 229960000723 ampicillin Drugs 0.000 description 1
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
• 230000003321 amplification Effects 0.000 description 1
• 238000010171 animal model Methods 0.000 description 1
• 239000005557 antagonist Substances 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 230000000692 anti-sense effect Effects 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 230000007503 antigenic stimulation Effects 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
• 239000005441 aurora Substances 0.000 description 1
• 201000008680 babesiosis Diseases 0.000 description 1
• 208000022362 bacterial infectious disease Diseases 0.000 description 1
• 229930192649 bafilomycin Natural products 0.000 description 1
• XDHNQDDQEHDUTM-UHFFFAOYSA-N bafliomycin A1 Natural products COC1C=CC=C(C)CC(C)C(O)C(C)C=C(C)C=C(OC)C(=O)OC1C(C)C(O)C(C)C1(O)OC(C(C)C)C(C)C(O)C1 XDHNQDDQEHDUTM-UHFFFAOYSA-N 0.000 description 1
• 230000004888 barrier function Effects 0.000 description 1
• 102000006635 beta-lactamase Human genes 0.000 description 1
• 230000008827 biological function Effects 0.000 description 1
• 238000001574 biopsy Methods 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• 229930189065 blasticidin Natural products 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 229910052799 carbon Inorganic materials 0.000 description 1
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
• 239000000969 carrier Substances 0.000 description 1
• 230000015556 catabolic process Effects 0.000 description 1
• 230000003197 catalytic effect Effects 0.000 description 1
• 238000006555 catalytic reaction Methods 0.000 description 1
• 230000010261 cell growth Effects 0.000 description 1
• 239000006285 cell suspension Substances 0.000 description 1
• 230000007969 cellular immunity Effects 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 235000013339 cereals Nutrition 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 238000006243 chemical reaction Methods 0.000 description 1
• 239000003153 chemical reaction reagent Substances 0.000 description 1
• 102000021178 chitin binding proteins Human genes 0.000 description 1
• 108091011157 chitin binding proteins Proteins 0.000 description 1
• 229960003677 chloroquine Drugs 0.000 description 1
• WHTVZRBIWZFKQO-UHFFFAOYSA-N chloroquine Natural products ClC1=CC=C2C(NC(C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-UHFFFAOYSA-N 0.000 description 1
• 239000013611 chromosomal DNA Substances 0.000 description 1
• 230000002759 chromosomal effect Effects 0.000 description 1
• 229910017052 cobalt Inorganic materials 0.000 description 1
• 239000010941 cobalt Substances 0.000 description 1
• GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
• 238000004891 communication Methods 0.000 description 1
• 230000001268 conjugating effect Effects 0.000 description 1
• 230000021615 conjugation Effects 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 238000012136 culture method Methods 0.000 description 1
• 239000012228 culture supernatant Substances 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 125000000392 cycloalkenyl group Chemical group 0.000 description 1
• 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
• 125000000596 cyclohexenyl group Chemical group C1(=CCCCC1)* 0.000 description 1
• 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
• 125000002433 cyclopentenyl group Chemical group C1(=CCCC1)* 0.000 description 1
• 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
• 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 description 1
• 230000009089 cytolysis Effects 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 108010025838 dectin 1 Proteins 0.000 description 1
• 230000007547 defect Effects 0.000 description 1
• 238000006731 degradation reaction Methods 0.000 description 1
• 238000013461 design Methods 0.000 description 1
• 239000008121 dextrose Substances 0.000 description 1
• 230000003467 diminishing effect Effects 0.000 description 1
• 230000008034 disappearance Effects 0.000 description 1
• 208000037765 diseases and disorders Diseases 0.000 description 1
• BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
• 229910000397 disodium phosphate Inorganic materials 0.000 description 1
• 235000019800 disodium phosphate Nutrition 0.000 description 1
• 239000012153 distilled water Substances 0.000 description 1
• 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 241001493065 dsRNA viruses Species 0.000 description 1
• 108010057988 ecdysone receptor Proteins 0.000 description 1
• 244000078703 ectoparasite Species 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 230000009881 electrostatic interaction Effects 0.000 description 1
• 238000010828 elution Methods 0.000 description 1
• 210000001163 endosome Anatomy 0.000 description 1
• 230000002708 enhancing effect Effects 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• DANUORFCFTYTSZ-UHFFFAOYSA-N epinigericin Natural products O1C2(C(CC(C)(O2)C2OC(C)(CC2)C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)C)C(C)C(OC)CC1CC1CCC(C)C(C(C)C(O)=O)O1 DANUORFCFTYTSZ-UHFFFAOYSA-N 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 210000003743 erythrocyte Anatomy 0.000 description 1
• 210000003238 esophagus Anatomy 0.000 description 1
• 108010038795 estrogen receptors Proteins 0.000 description 1
• 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
• 125000005469 ethylenyl group Chemical group 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 239000013613 expression plasmid Substances 0.000 description 1
• 239000003889 eye drop Substances 0.000 description 1
• 229940012356 eye drops Drugs 0.000 description 1
• 230000012953 feeding on blood of other organism Effects 0.000 description 1
• 239000012894 fetal calf serum Substances 0.000 description 1
• 239000000706 filtrate Substances 0.000 description 1
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 239000012634 fragment Substances 0.000 description 1
• 239000012737 fresh medium Substances 0.000 description 1
• 108700004026 gag Genes Proteins 0.000 description 1
• 230000030279 gene silencing Effects 0.000 description 1
• 230000009368 gene silencing by RNA Effects 0.000 description 1
• 108060003196 globin Proteins 0.000 description 1
• 102000018146 globin Human genes 0.000 description 1
• 229930195712 glutamate Natural products 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
• 208000006454 hepatitis Diseases 0.000 description 1
• 231100000283 hepatitis Toxicity 0.000 description 1
• 102000044493 human CDCA4 Human genes 0.000 description 1
• 230000002209 hydrophobic effect Effects 0.000 description 1
• 230000002519 immonomodulatory effect Effects 0.000 description 1
• 230000036039 immunity Effects 0.000 description 1
• 102000018358 immunoglobulin Human genes 0.000 description 1
• 230000004957 immunoregulator effect Effects 0.000 description 1
• 238000009169 immunotherapy Methods 0.000 description 1
• 230000000415 inactivating effect Effects 0.000 description 1
• 238000011534 incubation Methods 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 206010022000 influenza Diseases 0.000 description 1
• 208000037797 influenza A Diseases 0.000 description 1
• 239000004615 ingredient Substances 0.000 description 1
• 230000005764 inhibitory process Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 229940125396 insulin Drugs 0.000 description 1
• 229940079322 interferon Drugs 0.000 description 1
• 108010074108 interleukin-21 Proteins 0.000 description 1
• 108090000237 interleukin-24 Proteins 0.000 description 1
• 102000003898 interleukin-24 Human genes 0.000 description 1
• 229940047122 interleukins Drugs 0.000 description 1
• 230000000968 intestinal effect Effects 0.000 description 1
• 201000008267 intestinal tuberculosis Diseases 0.000 description 1
• 238000007912 intraperitoneal administration Methods 0.000 description 1
• 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
• 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
• 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
• 208000032839 leukemia Diseases 0.000 description 1
• 238000007798 limiting dilution analysis Methods 0.000 description 1
• 239000002502 liposome Substances 0.000 description 1
• 210000004185 liver Anatomy 0.000 description 1
• 210000005229 liver cell Anatomy 0.000 description 1
• 230000002132 lysosomal effect Effects 0.000 description 1
• 235000009973 maize Nutrition 0.000 description 1
• 201000004792 malaria Diseases 0.000 description 1
• 230000003211 malignant effect Effects 0.000 description 1
• 125000002960 margaryl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
• 230000034217 membrane fusion Effects 0.000 description 1
• 108020004999 messenger RNA Proteins 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• 150000002739 metals Chemical class 0.000 description 1
• 229960000485 methotrexate Drugs 0.000 description 1
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
• 230000011987 methylation Effects 0.000 description 1
• 238000007069 methylation reaction Methods 0.000 description 1
• 238000012737 microarray-based gene expression Methods 0.000 description 1
• 238000000520 microinjection Methods 0.000 description 1
• 238000000386 microscopy Methods 0.000 description 1
• 230000005012 migration Effects 0.000 description 1
• 238000013508 migration Methods 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 238000000302 molecular modelling Methods 0.000 description 1
• 229960005358 monensin Drugs 0.000 description 1
• GAOZTHIDHYLHMS-KEOBGNEYSA-N monensin A Chemical compound C([C@@](O1)(C)[C@H]2CC[C@@](O2)(CC)[C@H]2[C@H](C[C@@H](O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C[C@@]21C[C@H](O)[C@@H](C)[C@@H]([C@@H](C)[C@@H](OC)[C@H](C)C(O)=O)O2 GAOZTHIDHYLHMS-KEOBGNEYSA-N 0.000 description 1
• 238000012243 multiplex automated genomic engineering Methods 0.000 description 1
• 125000001446 muramyl group Chemical group N[C@@H](C=O)[C@@H](O[C@@H](C(=O)*)C)[C@H](O)[C@H](O)CO 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• 201000000050 myeloid neoplasm Diseases 0.000 description 1
• 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
• 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 125000001280 n-hexyl group Chemical group C(CCCCC)* 0.000 description 1
• 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 230000001537 neural effect Effects 0.000 description 1
• 230000007935 neutral effect Effects 0.000 description 1
• 229910052759 nickel Inorganic materials 0.000 description 1
• DANUORFCFTYTSZ-BIBFWWMMSA-N nigericin Chemical compound C([C@@H]1C[C@H]([C@H]([C@]2([C@@H](C[C@](C)(O2)C2O[C@@](C)(CC2)C2[C@H](CC(O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C)O1)C)OC)[C@H]1CC[C@H](C)C([C@@H](C)C(O)=O)O1 DANUORFCFTYTSZ-BIBFWWMMSA-N 0.000 description 1
• 108091027963 non-coding RNA Proteins 0.000 description 1
• 102000042567 non-coding RNA Human genes 0.000 description 1
• 230000009871 nonspecific binding Effects 0.000 description 1
• 238000010899 nucleation Methods 0.000 description 1
• 238000003199 nucleic acid amplification method Methods 0.000 description 1
• 210000004940 nucleus Anatomy 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 210000000056 organ Anatomy 0.000 description 1
• 230000008212 organismal development Effects 0.000 description 1
• 230000008520 organization Effects 0.000 description 1
• VYNDHICBIRRPFP-UHFFFAOYSA-N pacific blue Chemical compound FC1=C(O)C(F)=C2OC(=O)C(C(=O)O)=CC2=C1 VYNDHICBIRRPFP-UHFFFAOYSA-N 0.000 description 1
• 238000012856 packing Methods 0.000 description 1
• 229920002866 paraformaldehyde Polymers 0.000 description 1
• 244000045947 parasite Species 0.000 description 1
• 230000003071 parasitic effect Effects 0.000 description 1
• 244000052769 pathogen Species 0.000 description 1
• 230000007918 pathogenicity Effects 0.000 description 1
• 230000037361 pathway Effects 0.000 description 1
• 239000000312 peanut oil Substances 0.000 description 1
• 239000008188 pellet Substances 0.000 description 1
• 210000005259 peripheral blood Anatomy 0.000 description 1
• 239000011886 peripheral blood Substances 0.000 description 1
• 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
• 230000002093 peripheral effect Effects 0.000 description 1
• 206010034674 peritonitis Diseases 0.000 description 1
• 238000007747 plating Methods 0.000 description 1
• 201000000317 pneumocystosis Diseases 0.000 description 1
• 108700004029 pol Genes Proteins 0.000 description 1
• 101150088264 pol gene Proteins 0.000 description 1
• 229920002643 polyglutamic acid Polymers 0.000 description 1
• 229920000656 polylysine Polymers 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• 230000027317 positive regulation of immune response Effects 0.000 description 1
• 229910052700 potassium Inorganic materials 0.000 description 1
• 239000011591 potassium Substances 0.000 description 1
• 239000000843 powder Substances 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• 238000003825 pressing Methods 0.000 description 1
• 230000010469 pro-virus integration Effects 0.000 description 1
• 238000012545 processing Methods 0.000 description 1
• 229960003387 progesterone Drugs 0.000 description 1
• 239000000186 progesterone Substances 0.000 description 1
• 108090000468 progesterone receptors Proteins 0.000 description 1
• 125000005470 propylenyl group Chemical group 0.000 description 1
• 125000002568 propynyl group Chemical group [*]C#CC([H])([H])[H] 0.000 description 1
• 210000002307 prostate Anatomy 0.000 description 1
• 235000019833 protease Nutrition 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 230000004853 protein function Effects 0.000 description 1
• 230000007026 protein scission Effects 0.000 description 1
• 210000001938 protoplast Anatomy 0.000 description 1
• 230000005855 radiation Effects 0.000 description 1
• 230000006798 recombination Effects 0.000 description 1
• 238000005215 recombination Methods 0.000 description 1
• 230000008929 regeneration Effects 0.000 description 1
• 238000011069 regeneration method Methods 0.000 description 1
• 238000009877 rendering Methods 0.000 description 1
• 230000008261 resistance mechanism Effects 0.000 description 1
• 230000008593 response to virus Effects 0.000 description 1
• 150000004492 retinoid derivatives Chemical class 0.000 description 1
• 102000027483 retinoid hormone receptors Human genes 0.000 description 1
• 108091008679 retinoid hormone receptors Proteins 0.000 description 1
• 102220128858 rs200860772 Human genes 0.000 description 1
• 150000003839 salts Chemical class 0.000 description 1
• 229940043230 sarcosine Drugs 0.000 description 1
• 201000004409 schistosomiasis Diseases 0.000 description 1
• 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
• 238000012764 semi-quantitative analysis Methods 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 229910052708 sodium Inorganic materials 0.000 description 1
• 239000011734 sodium Substances 0.000 description 1
• 239000011780 sodium chloride Substances 0.000 description 1
• 239000007787 solid Substances 0.000 description 1
• 210000004989 spleen cell Anatomy 0.000 description 1
• 230000006641 stabilisation Effects 0.000 description 1
• 238000011105 stabilization Methods 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
• 238000011146 sterile filtration Methods 0.000 description 1
• 239000008223 sterile water Substances 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 239000011550 stock solution Substances 0.000 description 1
• 230000035892 strand transfer Effects 0.000 description 1
• 238000010254 subcutaneous injection Methods 0.000 description 1
• 239000007929 subcutaneous injection Substances 0.000 description 1
• 239000000758 substrate Substances 0.000 description 1
• 208000024891 symptom Diseases 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1
• 238000010189 synthetic method Methods 0.000 description 1
• 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
• 210000001550 testis Anatomy 0.000 description 1
• 229940124597 therapeutic agent Drugs 0.000 description 1
• 108060008226 thioredoxin Proteins 0.000 description 1
• 229940094937 thioredoxin Drugs 0.000 description 1
• 102000004217 thyroid hormone receptors Human genes 0.000 description 1
• 108090000721 thyroid hormone receptors Proteins 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• 108700012359 toxins Proteins 0.000 description 1
• 108091007466 transmembrane glycoproteins Proteins 0.000 description 1
• 230000032258 transport Effects 0.000 description 1
• 239000013638 trimer Substances 0.000 description 1
• 201000008827 tuberculosis Diseases 0.000 description 1
• 231100000397 ulcer Toxicity 0.000 description 1
• 238000005199 ultracentrifugation Methods 0.000 description 1
• 241000712461 unidentified influenza virus Species 0.000 description 1
• 230000003827 upregulation Effects 0.000 description 1
• 238000011144 upstream manufacturing Methods 0.000 description 1
• 210000003462 vein Anatomy 0.000 description 1
• 230000035899 viability Effects 0.000 description 1
• 230000007442 viral DNA synthesis Effects 0.000 description 1
• 238000003260 vortexing Methods 0.000 description 1
• 229940051021 yellow-fever virus Drugs 0.000 description 1
• 230000004572 zinc-binding Effects 0.000 description 1