JP2013059337A - 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊 - Google Patents

体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊 Download PDF

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JP2013059337A
JP2013059337A JP2012248660A JP2012248660A JP2013059337A JP 2013059337 A JP2013059337 A JP 2013059337A JP 2012248660 A JP2012248660 A JP 2012248660A JP 2012248660 A JP2012248660 A JP 2012248660A JP 2013059337 A JP2013059337 A JP 2013059337A
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cells
cell
somatic cells
culture
cell mass
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Shigeyoshi Fujiwara
重良 藤原
Jiro Kishimoto
治郎 岸本
Tsutomu Soma
勤 相馬
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Shiseido Co Ltd
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Shiseido Co Ltd
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0666—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled

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  • Oral & Maxillofacial Surgery (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2012248660A 2006-06-27 2012-11-12 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊 Pending JP2013059337A (ja)

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JP2012248660A JP2013059337A (ja) 2006-06-27 2012-11-12 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊

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JP2006176763 2006-06-27
JP2006176763 2006-06-27
JP2012248660A JP2013059337A (ja) 2006-06-27 2012-11-12 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊

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JP2013019652A Pending JP2013078344A (ja) 2006-06-27 2013-02-04 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊
JP2015131234A Active JP5976172B2 (ja) 2006-06-27 2015-06-30 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊

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JP2015131234A Active JP5976172B2 (ja) 2006-06-27 2015-06-30 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊

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US (3) US8563309B2 (enExample)
EP (2) EP2034011B1 (enExample)
JP (3) JP2013059337A (enExample)
KR (1) KR101472594B1 (enExample)
CN (1) CN101479376A (enExample)
ES (2) ES2641528T3 (enExample)
WO (1) WO2008001938A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009086000A2 (en) * 2007-12-19 2009-07-09 Aderans Research Institute, Inc. Biomarkers for trichogenicity
CN101658518B (zh) * 2009-08-19 2011-06-08 新疆奥斯曼生物化妆品有限公司 靛玉红用作制备育毛发的药物中的用途
GB201112922D0 (en) * 2011-07-27 2011-09-14 Univ Durham Micro-organ
AU2014259787B2 (en) * 2013-05-03 2018-03-08 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Skin substitutes and methods for hair follicle neogenesis
WO2015155738A2 (en) 2014-04-09 2015-10-15 Christopher Rudd Use of gsk-3 inhibitors or activators which modulate pd-1 or t-bet expression to modulate t cell immunity
JP6218238B2 (ja) * 2014-07-10 2017-10-25 国立大学法人大阪大学 人工皮膚及びその製造方法
CN120924475A (zh) 2014-09-18 2025-11-11 北卡罗来纳大学 哺乳动物肺球体和肺球体细胞及其应用
JP6425319B2 (ja) 2015-10-30 2018-11-28 国立大学法人横浜国立大学 複数の再生毛包原基の製造方法、毛包組織含有シートの製造方法、毛包組織含有シート及び培養基板の使用
JP7150604B2 (ja) * 2016-01-28 2022-10-11 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 無血清合成のためのｗｎｔ組成物及び方法
FR3061207B1 (fr) * 2016-12-23 2023-03-10 Oreal Utilisation des cellules germinales pour la preparation d’un microfollicule pileux
KR101948396B1 (ko) * 2016-12-26 2019-02-14 동국대학교 산학협력단 특정 전자기파 처리를 통한 효율적 3d 중뇌 유사 오가노이드 제조 방법
CN110392686A (zh) 2016-12-30 2019-10-29 频率治疗公司 1h-吡咯-2,5-二酮化合物以及使用它们来诱导干/祖支持细胞自我更新的方法
JP2020516282A (ja) * 2017-04-11 2020-06-11 フリークエンシー セラピューティクス インコーポレイテッド 毛包幹細胞増殖のための方法
WO2019092667A1 (en) * 2017-11-10 2019-05-16 Vitroscreen S.R.L. Method for preparing three-dimensional scaffold-free microtissues for use in the preclinical screening of active substances
CN108486035B (zh) * 2018-03-26 2021-11-26 西北大学 一种三维类器官的液滴培养方法
JP7142840B2 (ja) 2018-05-16 2022-09-28 国立大学法人横浜国立大学 細胞含有ハイドロゲル体及びその製造方法
JP7246595B2 (ja) * 2018-11-08 2023-03-28 国立大学法人横浜国立大学 毛包原基、毛包原基の製造方法、及び毛包原基に含まれる細胞の活性化方法
JP7702229B2 (ja) * 2018-11-19 2025-07-03 株式会社 資生堂 毛包を有するヒト皮膚組織を再構成するための組成物、ヒト皮膚組織モデル動物およびその製造方法
KR102636511B1 (ko) * 2020-03-20 2024-02-14 주식회사 에이엔케이 기판 상에 형성된 모낭 세포 스페로이드 및 이의 제조방법
KR102636512B1 (ko) * 2020-03-20 2024-02-14 주식회사 에이엔케이 모유두 세포의 반복 시딩 및 배양을 통한 모유두 스페로이드의 제조방법
WO2022039165A1 (ja) 2020-08-18 2022-02-24 東ソー株式会社 多能性幹細胞の外胚葉、中胚葉及び内胚葉系細胞への分化誘導方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003082234A1 (fr) * 2002-03-28 2003-10-09 Shiseido Co., Ltd. Derives de phenylacetonitrile, tonique capillaire et preparations externes pour la peau contenant ces derives
JP2004248632A (ja) * 2003-02-21 2004-09-09 Osaka Industrial Promotion Organization 男性型脱毛症治療薬のスクリーニング方法、男性型脱毛症治療薬の製造方法および男性型脱毛症治療薬。
JP2005021171A (ja) * 2004-10-04 2005-01-27 Shiseido Co Ltd ヒト毛芽の活性化作用の検出方法
JP2005237273A (ja) * 2004-02-26 2005-09-08 Shiseido Co Ltd 育毛剤の評価方法
JP2006083082A (ja) * 2004-09-15 2006-03-30 National Institute Of Advanced Industrial & Technology 毛髪成長促進剤、発毛促進剤及び脱毛症治療剤

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4813734B2 (ja) * 2000-03-31 2011-11-09 ザ ジェネラル ホスピタル コーポレイション 毛の成長を調節する方法
AU2004317828B2 (en) * 2004-03-31 2010-06-24 Reliance Life Sciences Pvt Ltd Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003082234A1 (fr) * 2002-03-28 2003-10-09 Shiseido Co., Ltd. Derives de phenylacetonitrile, tonique capillaire et preparations externes pour la peau contenant ces derives
JP2004248632A (ja) * 2003-02-21 2004-09-09 Osaka Industrial Promotion Organization 男性型脱毛症治療薬のスクリーニング方法、男性型脱毛症治療薬の製造方法および男性型脱毛症治療薬。
JP2005237273A (ja) * 2004-02-26 2005-09-08 Shiseido Co Ltd 育毛剤の評価方法
JP2006083082A (ja) * 2004-09-15 2006-03-30 National Institute Of Advanced Industrial & Technology 毛髪成長促進剤、発毛促進剤及び脱毛症治療剤
JP2005021171A (ja) * 2004-10-04 2005-01-27 Shiseido Co Ltd ヒト毛芽の活性化作用の検出方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6014021222; ＫＩＳＨＩＭＯＴＯ，Ｊ．　ｅｔ　ａｌ．: 'Ｗｎｔ　ｓｉｇｎａｌｉｎｇ　ｍａｉｎｔａｉｎｓ　ｔｈｅ　ｈａｉｒ-ｉｎｄｕｃｉｎｇ　ａｃｔｉｖｉｔｙ　ｏｆ　ｔｈｅ　ｄｅｒｍａｌ　ｐａｐｉｌｌａ．' Ｇｅｎｅｓ　Ｄｅｖ． Ｖｏｌ．１４，　Ｎｏ．１０, 20000515, ｐｐ．１１８１-５ *
JPN6014021226; ＳＯＭＡ，Ｔ．　ｅｔ　ａｌ．: 'Ｇｅｎｅｒａｔｉｏｎ　ａｎｄ　ｃｈａｒａｃｔｅｒｉｚａｔｉｏｎ　ｏｆ　ｔｈｒｅｅ-ｄｉｍｅｎｓｉｏｎａｌ　ｈｕｍａｎ　ｈａｉｒ　ｆｏｌｌｉｃｌｅ　ｍｏｄｅｌ　ｕｓｉｎｇ　ｈａｎｇｉｎｇ　ｄｒｏｐ　ｃｕ' Ｊ．　Ｉｎｖｅｓｔ．　Ｄｅｒｍａｔｏｌ． Ｖｏｌ．１２８，　Ｓｕｐｐｌ, 200804, ｐ．Ｓ１５０ *

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EP2455111B1 (en) 2017-08-16
US20090280469A1 (en) 2009-11-12
US20160160185A1 (en) 2016-06-09
US10344266B2 (en) 2019-07-09
CN101479376A (zh) 2009-07-08
EP2034011A4 (en) 2009-11-25
EP2455111A2 (en) 2012-05-23
JP2015165823A (ja) 2015-09-24
US20140106432A1 (en) 2014-04-17
ES2641528T3 (es) 2017-11-10
JP5976172B2 (ja) 2016-08-23
WO2008001938A1 (en) 2008-01-03
KR101472594B1 (ko) 2014-12-15
ES2419232T3 (es) 2013-08-20
EP2455111A3 (en) 2012-06-13
EP2034011A1 (en) 2009-03-11
EP2034011B1 (en) 2013-05-22
US8563309B2 (en) 2013-10-22
KR20090021340A (ko) 2009-03-03
JP2013078344A (ja) 2013-05-02

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