JP2012529912A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2012529912A5 JP2012529912A5 JP2012516171A JP2012516171A JP2012529912A5 JP 2012529912 A5 JP2012529912 A5 JP 2012529912A5 JP 2012516171 A JP2012516171 A JP 2012516171A JP 2012516171 A JP2012516171 A JP 2012516171A JP 2012529912 A5 JP2012529912 A5 JP 2012529912A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- host cell
- polypeptide
- compared
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001184 polypeptide Polymers 0.000 claims description 53
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 53
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 53
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 34
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 230000000813 microbial effect Effects 0.000 claims description 26
- 235000020978 long-chain polyunsaturated fatty acids Nutrition 0.000 claims description 20
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 18
- 150000001413 amino acids Chemical class 0.000 claims description 18
- 235000020669 docosahexaenoic acid Nutrition 0.000 claims description 18
- 229940090949 docosahexaenoic acid Drugs 0.000 claims description 18
- 102000018659 1-Acylglycerophosphocholine O-Acyltransferase Human genes 0.000 claims description 17
- 108010052187 1-Acylglycerophosphocholine O-Acyltransferase Proteins 0.000 claims description 17
- 125000002252 acyl group Chemical group 0.000 claims description 17
- 230000000694 effects Effects 0.000 claims description 17
- 235000020673 eicosapentaenoic acid Nutrition 0.000 claims description 17
- 102000040430 polynucleotide Human genes 0.000 claims description 17
- 108091033319 polynucleotide Proteins 0.000 claims description 17
- 239000002157 polynucleotide Substances 0.000 claims description 17
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 claims description 16
- 229960005135 eicosapentaenoic acid Drugs 0.000 claims description 16
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 claims description 16
- 230000001105 regulatory effect Effects 0.000 claims description 12
- 102000004223 1-acyl-sn-glycerol-3-phosphate acyltransferase Human genes 0.000 claims description 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 6
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 6
- 229930195729 fatty acid Natural products 0.000 claims description 6
- 239000000194 fatty acid Substances 0.000 claims description 6
- 150000004665 fatty acids Chemical class 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 101710124165 1-acyl-sn-glycerol-3-phosphate acyltransferase Proteins 0.000 claims description 4
- 101710097496 Lysophospholipid acyltransferase Proteins 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 108700013165 1-acyl-sn-glycerol-3-phosphate acyltransferase family Proteins 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 50
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 241000235015 Yarrowia lipolytica Species 0.000 description 5
- 244000005700 microbiome Species 0.000 description 3
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- XSXIVVZCUAHUJO-AVQMFFATSA-N (11e,14e)-icosa-11,14-dienoic acid Chemical compound CCCCC\C=C\C\C=C\CCCCCCCCCC(O)=O XSXIVVZCUAHUJO-AVQMFFATSA-N 0.000 description 1
- BBWMTEYXFFWPIF-CJBMEHDJSA-N (2e,4e,6e)-icosa-2,4,6-trienoic acid Chemical compound CCCCCCCCCCCCC\C=C\C=C\C=C\C(O)=O BBWMTEYXFFWPIF-CJBMEHDJSA-N 0.000 description 1
- FPRKGXIOSIUDSE-SYACGTDESA-N (2z,4z,6z,8z)-docosa-2,4,6,8-tetraenoic acid Chemical compound CCCCCCCCCCCCC\C=C/C=C\C=C/C=C\C(O)=O FPRKGXIOSIUDSE-SYACGTDESA-N 0.000 description 1
- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 1
- HOBAELRKJCKHQD-UHFFFAOYSA-N (8Z,11Z,14Z)-8,11,14-eicosatrienoic acid Natural products CCCCCC=CCC=CCC=CCCCCCCC(O)=O HOBAELRKJCKHQD-UHFFFAOYSA-N 0.000 description 1
- 235000021298 Dihomo-γ-linolenic acid Nutrition 0.000 description 1
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 1
- 235000021292 Docosatetraenoic acid Nutrition 0.000 description 1
- 235000021297 Eicosadienoic acid Nutrition 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- HOBAELRKJCKHQD-QNEBEIHSSA-N dihomo-γ-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCCCC(O)=O HOBAELRKJCKHQD-QNEBEIHSSA-N 0.000 description 1
- IQLUYYHUNSSHIY-HZUMYPAESA-N eicosatetraenoic acid Chemical compound CCCCCCCCCCC\C=C\C=C\C=C\C=C\C(O)=O IQLUYYHUNSSHIY-HZUMYPAESA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18735909P | 2009-06-16 | 2009-06-16 | |
| US61/187,359 | 2009-06-16 | ||
| PCT/US2010/038527 WO2010147900A1 (en) | 2009-06-16 | 2010-06-14 | IMPROVEMENT OF LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED FATTY ACID BIOSYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYLTRANSFERASES |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012529912A JP2012529912A (ja) | 2012-11-29 |
| JP2012529912A5 true JP2012529912A5 (enExample) | 2013-08-01 |
| JP5931721B2 JP5931721B2 (ja) | 2016-06-08 |
Family
ID=43356702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012516171A Expired - Fee Related JP5931721B2 (ja) | 2009-06-16 | 2010-06-14 | アシルＣｏＡリゾリン脂質アシルトランスフェラーゼの発現による長鎖オメガ−３及びオメガ−６多価不飽和脂肪酸生合成の改良 |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP2443248B1 (enExample) |
| JP (1) | JP5931721B2 (enExample) |
| CN (1) | CN102459627B (enExample) |
| AU (1) | AU2010260227B2 (enExample) |
| BR (1) | BRPI1009690A2 (enExample) |
| CA (1) | CA2763424C (enExample) |
| CL (1) | CL2011003161A1 (enExample) |
| DK (1) | DK2443248T3 (enExample) |
| NO (1) | NO2443248T3 (enExample) |
| WO (1) | WO2010147900A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2442666T3 (da) | 2009-06-16 | 2015-07-13 | Du Pont | Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica |
| IL313986B1 (en) | 2012-02-17 | 2026-05-01 | Alcresta Inc | Methods, compositions and devices for supplying dietary fatty acid needs |
| US8946460B2 (en) | 2012-06-15 | 2015-02-03 | Commonwealth Scientific And Industrial Research Organisation | Process for producing polyunsaturated fatty acids in an esterified form |
| WO2013192002A1 (en) * | 2012-06-19 | 2013-12-27 | E. I. Du Pont De Nemours And Company | IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES |
| WO2015196250A1 (en) | 2014-06-27 | 2015-12-30 | Commonwealth Scientific And Industrial Research Organisation | Lipid comprising docosapentaenoic acid |
| KR102386838B1 (ko) * | 2013-12-18 | 2022-04-15 | 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 | 장쇄 다중불포화 지방산을 포함하는 지질 |
| CN113980730B (zh) * | 2014-06-27 | 2025-05-27 | 联邦科学技术研究组织 | 包含二十二碳五烯酸的提取的植物脂质 |
| EA037184B1 (ru) * | 2014-06-27 | 2021-02-16 | Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн | Липид, содержащий докозапентаеновую кислоту |
| US10258590B2 (en) | 2015-10-14 | 2019-04-16 | Alcresta Therapeutics, Inc. | Enteral feeding device and related methods of use |
| JP7097014B2 (ja) * | 2018-02-13 | 2022-07-07 | 国立大学法人富山大学 | 藻類及びその使用 |
| EP3586640A1 (en) | 2018-06-21 | 2020-01-01 | Nuseed Pty Ltd | Dha enriched polyunsaturated fatty acid compositions |
| EP3586641A1 (en) | 2018-06-21 | 2020-01-01 | Nuseed Pty Ltd | Dha enriched polyunsaturated fatty acid compositions |
| EP3586643A1 (en) | 2018-06-21 | 2020-01-01 | Nuseed Pty Ltd | Dha enriched polyunsaturated fatty acid compositions |
| EP3586642A1 (en) | 2018-06-21 | 2020-01-01 | Nuseed Pty Ltd | Ala enriched polyunsaturated fatty acid compositions |
| IL300525A (en) * | 2020-08-11 | 2023-04-01 | Viva Star Biosciences Ltd | Triazole-pyridinyl-substituted azacyclohexyl acetic acid compounds as LPA receptor antagonists |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4792523A (en) | 1984-08-31 | 1988-12-20 | Cetus Corporation | 3'Expression enhancing fragments and method |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| TWI377253B (en) | 2001-04-16 | 2012-11-21 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
| BRPI0407138A (pt) | 2003-02-27 | 2006-01-10 | Basf Plant Science Gmbh | Sequência de ácido nucleico isolada, sequência de aminoácido, construção de gene, vetor, organismo transgênico não humano, processo para produzir ácidos graxos poliinsaturados, óleo, lipìdeo, ou um ácido graxo poliinsaturado ou uma fração dos mesmos, composições de óleo, de lipìdeos, ou de ácido graxo, e, uso do óleo, lipìdeos ou ácidos graxos ou de composições de óleo, de lipìdeos ou de ácido graxo |
| AU2004225838B2 (en) | 2003-03-31 | 2009-11-05 | University Of Bristol | Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids |
| US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7504259B2 (en) | 2003-11-12 | 2009-03-17 | E. I. Du Pont De Nemours And Company | Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast |
| EP1758991B1 (en) | 2004-06-25 | 2013-01-16 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US7189559B2 (en) * | 2004-11-04 | 2007-03-13 | E. I. Du Pont De Nemours And Company | Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| DE102004062294A1 (de) | 2004-12-23 | 2006-07-06 | Basf Plant Science Gmbh | Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen |
| AU2006227165B2 (en) | 2005-03-18 | 2011-11-10 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| US7470532B2 (en) | 2005-10-19 | 2008-12-30 | E.I. Du Pont De Nemours And Company | Mortierella alpina C16/18 fatty acid elongase |
| CA2624661C (en) | 2005-11-23 | 2015-06-30 | E.I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
| AU2007299219A1 (en) * | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| DK2087105T3 (da) | 2006-10-30 | 2015-05-26 | Du Pont | Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer |
| US7709239B2 (en) | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| US8846374B2 (en) | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
| US7732155B2 (en) * | 2006-12-13 | 2010-06-08 | National Research Council Of Canada | Methods for identifying lysophosphatidylcholine acyltransferases |
| BRPI0808577A2 (pt) | 2007-04-03 | 2019-09-24 | E.I. Du Pont De Nemours And Company | multienzima, polinuleotídeos isolados, construção recombinante, célula hospedeira, yarrowia sp. transformada, método de transformaçao celular, método para a produção de uma planta transformada, de leveduras, planta, sementes, óleos, produtos alimentícios ou rações, bebidas, moléculas isoladas de ácido nucléico, método de produção de uma multienzima, método para alterar o perfil de ácidos graxos, progênie, células hospedeiras microbianas recombinantes, métodos para conversão de ácido linoléico para ácido dihomo gamma-linolênico, ácido a-linolênico para ácido eicosatrienóico, ácido eicosapentaenóico para ácido docosahexaenóico, ácido araquidônico para ácido docosapentaenóico, método para a identificação de um polipeptídeo |
| WO2009001315A2 (en) * | 2007-06-26 | 2008-12-31 | Staahl Ulf | Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine |
| JP5346290B2 (ja) | 2007-07-23 | 2013-11-20 | サントリーホールディングス株式会社 | 新規な脂肪酸組成を有する脂肪酸組成物 |
| CN101883843B (zh) | 2007-10-03 | 2016-03-02 | 纳幕尔杜邦公司 | 破坏过氧化物酶体生物合成因子蛋白(pex)以改变含油真核生物中多不饱和脂肪酸和总脂质含量 |
| US8323935B2 (en) * | 2007-10-03 | 2012-12-04 | E I Du Pont De Nemours And Company | Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production |
-
2010
- 2010-06-14 JP JP2012516171A patent/JP5931721B2/ja not_active Expired - Fee Related
- 2010-06-14 WO PCT/US2010/038527 patent/WO2010147900A1/en not_active Ceased
- 2010-06-14 BR BRPI1009690-6A patent/BRPI1009690A2/pt not_active Application Discontinuation
- 2010-06-14 AU AU2010260227A patent/AU2010260227B2/en not_active Ceased
- 2010-06-14 EP EP10790004.5A patent/EP2443248B1/en not_active Not-in-force
- 2010-06-14 CA CA2763424A patent/CA2763424C/en not_active Expired - Fee Related
- 2010-06-14 NO NO10790004A patent/NO2443248T3/no unknown
- 2010-06-14 CN CN201080026825.0A patent/CN102459627B/zh not_active Expired - Fee Related
- 2010-06-14 DK DK10790004.5T patent/DK2443248T3/en active
-
2011
- 2011-12-15 CL CL2011003161A patent/CL2011003161A1/es unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7465565B2 (en) | Methods of increasing triacylglycerol content in oleaginous yeasts via expression of acyltransferases | |
| Sakuradani et al. | Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4 | |
| CN100591773C (zh) | 在含油酵母中生产多不饱和脂肪酸 | |
| US7202356B2 (en) | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast | |
| US7259255B2 (en) | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast | |
| JP5931721B2 (ja) | アシルＣｏＡリゾリン脂質アシルトランスフェラーゼの発現による長鎖オメガ−３及びオメガ−６多価不飽和脂肪酸生合成の改良 | |
| JP2007504839A5 (enExample) | ||
| JP2010527244A5 (enExample) | ||
| JP2012502659A5 (enExample) | ||
| JP2013535987A5 (enExample) | ||
| JP2009534032A5 (enExample) | ||
| EP2516641A1 (en) | Method for the production of very long chain fatty acids (vlcfa) by fermentation with a recombinant yarrowia sp | |
| JP2014519810A (ja) | 藻類のデサチュラーゼ | |
| JP2012530182A5 (enExample) | ||
| US7459546B2 (en) | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast | |
| JP2012529913A5 (enExample) | ||
| JP2013541328A5 (enExample) | ||
| JP2016502852A (ja) | 乾燥細胞重量で少なくとも２８％のエイコサペンタエン酸を産生する組換え微生物細胞 | |
| CN111575301A (zh) | 一种非从头合成的卷枝毛霉产油细胞工厂构建及其发酵技术 | |
| US20140335578A1 (en) | Integrated biodiesel process | |
| Zhu et al. | Production of omega-3 and omega-6 polyunsaturated fatty acids by metabolic engineering of Yarrowia lipolytica | |
| Lee et al. | Identification of Δ9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris | |
| JP2016502851A (ja) | 微生物細胞中における脂質生成を改変するためのｓｏｕ２ソルビトール利用タンパク質をコードするポリヌクレオチドの下方制御 | |
| CN119931853B (zh) | 高效生产dha的裂殖壶菌基因工程菌株、构建方法和应用 | |
| US10131897B2 (en) | Molecules associated with fatty acid biosynthetic pathways and uses thereof |