AU2010260227B2 - Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases - Google Patents

Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases Download PDF

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AU2010260227B2
AU2010260227B2 AU2010260227A AU2010260227A AU2010260227B2 AU 2010260227 B2 AU2010260227 B2 AU 2010260227B2 AU 2010260227 A AU2010260227 A AU 2010260227A AU 2010260227 A AU2010260227 A AU 2010260227A AU 2010260227 B2 AU2010260227 B2 AU 2010260227B2
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host cell
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AU2010260227A1 (en
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Narendra S. Yadav
Hongxiang Zhang
Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
AU2010260227A 2009-06-16 2010-06-14 Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases Ceased AU2010260227B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18735909P 2009-06-16 2009-06-16
US61/187,359 2009-06-16
PCT/US2010/038527 WO2010147900A1 (en) 2009-06-16 2010-06-14 IMPROVEMENT OF LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED FATTY ACID BIOSYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYLTRANSFERASES

Publications (2)

Publication Number Publication Date
AU2010260227A1 AU2010260227A1 (en) 2011-11-10
AU2010260227B2 true AU2010260227B2 (en) 2015-01-29

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AU2010260227A Ceased AU2010260227B2 (en) 2009-06-16 2010-06-14 Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases

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EP (1) EP2443248B1 (enExample)
JP (1) JP5931721B2 (enExample)
CN (1) CN102459627B (enExample)
AU (1) AU2010260227B2 (enExample)
BR (1) BRPI1009690A2 (enExample)
CA (1) CA2763424C (enExample)
CL (1) CL2011003161A1 (enExample)
DK (1) DK2443248T3 (enExample)
NO (1) NO2443248T3 (enExample)
WO (1) WO2010147900A1 (enExample)

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DK2442666T3 (da) 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
IL313986B1 (en) 2012-02-17 2026-05-01 Alcresta Inc Methods, compositions and devices for supplying dietary fatty acid needs
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form
WO2013192002A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
KR102386838B1 (ko) * 2013-12-18 2022-04-15 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 장쇄 다중불포화 지방산을 포함하는 지질
CN113980730B (zh) * 2014-06-27 2025-05-27 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质
EA037184B1 (ru) * 2014-06-27 2021-02-16 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Липид, содержащий докозапентаеновую кислоту
US10258590B2 (en) 2015-10-14 2019-04-16 Alcresta Therapeutics, Inc. Enteral feeding device and related methods of use
JP7097014B2 (ja) * 2018-02-13 2022-07-07 国立大学法人富山大学 藻類及びその使用
EP3586640A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
EP3586641A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
EP3586643A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
EP3586642A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Ala enriched polyunsaturated fatty acid compositions
IL300525A (en) * 2020-08-11 2023-04-01 Viva Star Biosciences Ltd Triazole-pyridinyl-substituted azacyclohexyl acetic acid compounds as LPA receptor antagonists

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WO2006052807A2 (en) * 2004-11-04 2006-05-18 E.I. Dupont De Nemours And Company A dna molecule of mortierella alpina lpaat homolog
WO2006052870A2 (en) * 2004-11-04 2006-05-18 E.I. Dupont De Nemours And Company High eicosapentaenoic acid producing strains of yarrowia lipolytica
WO2008076377A2 (en) * 2006-12-13 2008-06-26 National Research Council Of Canada Genes encoding a novel type of lysophophatidylcholine acyltransferases and their use to increase triacylglycerol production and/or modify fatty acid composition
WO2009001315A2 (en) * 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine

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TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
BRPI0407138A (pt) 2003-02-27 2006-01-10 Basf Plant Science Gmbh Sequência de ácido nucleico isolada, sequência de aminoácido, construção de gene, vetor, organismo transgênico não humano, processo para produzir ácidos graxos poliinsaturados, óleo, lipìdeo, ou um ácido graxo poliinsaturado ou uma fração dos mesmos, composições de óleo, de lipìdeos, ou de ácido graxo, e, uso do óleo, lipìdeos ou ácidos graxos ou de composições de óleo, de lipìdeos ou de ácido graxo
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WO2006052870A2 (en) * 2004-11-04 2006-05-18 E.I. Dupont De Nemours And Company High eicosapentaenoic acid producing strains of yarrowia lipolytica
WO2008076377A2 (en) * 2006-12-13 2008-06-26 National Research Council Of Canada Genes encoding a novel type of lysophophatidylcholine acyltransferases and their use to increase triacylglycerol production and/or modify fatty acid composition
WO2009001315A2 (en) * 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine

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Also Published As

Publication number Publication date
JP2012529912A (ja) 2012-11-29
BRPI1009690A2 (pt) 2015-08-25
NO2443248T3 (enExample) 2018-05-26
EP2443248B1 (en) 2017-12-27
AU2010260227A1 (en) 2011-11-10
JP5931721B2 (ja) 2016-06-08
DK2443248T3 (en) 2018-03-12
CA2763424A1 (en) 2010-12-23
WO2010147900A1 (en) 2010-12-23
CA2763424C (en) 2018-06-12
CN102459627B (zh) 2015-07-29
EP2443248A4 (en) 2013-07-10
EP2443248A1 (en) 2012-04-25
CN102459627A (zh) 2012-05-16
CL2011003161A1 (es) 2012-05-25

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