JP2011514144A5 - - Google Patents
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- Publication number
- JP2011514144A5 JP2011514144A5 JP2010544817A JP2010544817A JP2011514144A5 JP 2011514144 A5 JP2011514144 A5 JP 2011514144A5 JP 2010544817 A JP2010544817 A JP 2010544817A JP 2010544817 A JP2010544817 A JP 2010544817A JP 2011514144 A5 JP2011514144 A5 JP 2011514144A5
- Authority
- JP
- Japan
- Prior art keywords
- crei
- chain
- meganuclease
- crei meganuclease
- mutation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010050663 endodeoxyribonuclease CreI Proteins 0.000 claims 28
- 230000035772 mutation Effects 0.000 claims 9
- 108020004414 DNA Proteins 0.000 claims 6
- 108091033319 polynucleotide Proteins 0.000 claims 6
- 239000002157 polynucleotide Substances 0.000 claims 6
- 102000040430 polynucleotide Human genes 0.000 claims 6
- 210000004899 c-terminal region Anatomy 0.000 claims 5
- 239000012634 fragment Substances 0.000 claims 5
- 150000001413 amino acids Chemical class 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 239000013598 vector Substances 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 2
- 238000001727 in vivo Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000178 monomer Substances 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 230000008685 targeting Effects 0.000 claims 2
- 101100467529 Homo sapiens RAG1 gene Proteins 0.000 claims 1
- 102220527183 Immunoglobulin heavy joining 1_R51E_mutation Human genes 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 102220602177 Synaptotagmin-3_F54A_mutation Human genes 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000000539 dimer Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 238000010353 genetic engineering Methods 0.000 claims 1
- 239000000710 homodimer Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 102220223852 rs1060502978 Human genes 0.000 claims 1
- 102200081526 rs121913583 Human genes 0.000 claims 1
- 102200155456 rs35947557 Human genes 0.000 claims 1
- 102220122845 rs767335346 Human genes 0.000 claims 1
- 208000011580 syndromic disease Diseases 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IB2008/001331 WO2009095742A1 (en) | 2008-01-31 | 2008-01-31 | New i-crei derived single-chain meganuclease and uses thereof |
| PCT/IB2009/000439 WO2009095793A1 (en) | 2008-01-31 | 2009-02-02 | New i-crei derived single-chain meganuclease and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011514144A JP2011514144A (ja) | 2011-05-06 |
| JP2011514144A5 true JP2011514144A5 (https=) | 2012-03-08 |
Family
ID=39884323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010544817A Pending JP2011514144A (ja) | 2008-01-31 | 2009-02-02 | 新しいＩ−ＣｒｅＩ由来単鎖メガヌクレアーゼ及びその使用 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8927247B2 (https=) |
| EP (1) | EP2245147A1 (https=) |
| JP (1) | JP2011514144A (https=) |
| CN (1) | CN101970649B (https=) |
| AU (1) | AU2009208732A1 (https=) |
| CA (1) | CA2729124A1 (https=) |
| WO (2) | WO2009095742A1 (https=) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060078552A1 (en) † | 2002-03-15 | 2006-04-13 | Sylvain Arnould | Hybrid and single chain meganucleases and use thereof |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| EP2327771A1 (en) | 2005-03-15 | 2011-06-01 | Cellectis | I-crel meganuclease variants with modified specificity, method of preparation and uses thereof |
| WO2007060495A1 (en) * | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| WO2009019528A1 (en) * | 2007-08-03 | 2009-02-12 | Cellectis | Meganuclease variants cleaving a dna target sequence from the human interleukin-2 receptor gamma chain gene and uses thereof |
| EP2215223B1 (en) | 2007-10-31 | 2013-05-01 | Precision Biosciences, Inc. | Rationally-designed single-chain meganucleases with non-palindromic recognition sequences |
| WO2009074842A1 (en) * | 2007-12-13 | 2009-06-18 | Cellectis | Improved chimeric meganuclease enzymes and uses thereof |
| JP2012501641A (ja) * | 2008-09-08 | 2012-01-26 | セレクティス | グルタミンシンセターゼ遺伝子からのｄｎａ標的配列を切断するメガヌクレアーゼ変異型及びその使用 |
| EP2180058A1 (en) | 2008-10-23 | 2010-04-28 | Cellectis | Meganuclease recombination system |
| US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| US20120171191A1 (en) | 2009-05-26 | 2012-07-05 | Cellectis | Meganuclease variants cleaving the genome of a pathogenic non-integrating virus and uses thereof |
| WO2011021062A1 (en) * | 2009-08-21 | 2011-02-24 | Cellectis | Meganuclease variants cleaving a dna target sequence from the human lysosomal acid alpha-glucosidase gene and uses thereof |
| WO2011036640A2 (en) | 2009-09-24 | 2011-03-31 | Cellectis | Meganuclease reagents of uses thereof for treating genetic diseases caused by frame shift/non sense mutations |
| HUE041436T2 (hu) | 2009-12-10 | 2019-05-28 | Univ Minnesota | Tal-effektor-közvetített DNS-módosítás |
| AU2011219716A1 (en) | 2010-02-26 | 2012-09-27 | Cellectis | Use of endonucleases for inserting transgenes into safe harbor loci |
| US20130183282A1 (en) | 2010-05-12 | 2013-07-18 | Cellectis | Meganuclease variants cleaving a DNA target sequence from the rhodopsin gene and uses thereof |
| US20130145487A1 (en) | 2010-05-12 | 2013-06-06 | Cellectis | Meganuclease variants cleaving a dna target sequence from the dystrophin gene and uses thereof |
| JP6208580B2 (ja) | 2010-05-17 | 2017-10-04 | サンガモ セラピューティクス， インコーポレイテッド | 新規のｄｎａ結合タンパク質及びその使用 |
| US20130189759A1 (en) * | 2010-07-07 | 2013-07-25 | Cellectis | Meganucleases variants cleaving a dna target sequence in the nanog gene and uses thereof |
| WO2012010976A2 (en) | 2010-07-15 | 2012-01-26 | Cellectis | Meganuclease variants cleaving a dna target sequence in the tert gene and uses thereof |
| WO2012007848A2 (en) | 2010-07-16 | 2012-01-19 | Cellectis | Meganuclease variants cleaving a dna target sequence in the was gene and uses thereof |
| WO2012017329A2 (en) | 2010-08-02 | 2012-02-09 | Cellectis S.A. | Method for targeted genomic events in algae |
| EP2633040B1 (en) * | 2010-10-27 | 2019-07-10 | Cellectis | Method for increasing the efficiency of double-strand break-induced mutagenesis |
| US9044492B2 (en) | 2011-02-04 | 2015-06-02 | Cellectis Sa | Method for modulating the efficiency of double-strand break-induced mutagenesis |
| WO2012138901A1 (en) | 2011-04-05 | 2012-10-11 | Cellectis Sa | Method for enhancing rare-cutting endonuclease efficiency and uses thereof |
| EP2694089B1 (en) | 2011-04-05 | 2024-06-05 | Cellectis | New tale-protein scaffolds and uses thereof |
| KR102068107B1 (ko) | 2011-04-27 | 2020-01-20 | 아미리스 인코퍼레이티드 | 게놈 변형 방법 |
| WO2012158778A1 (en) * | 2011-05-16 | 2012-11-22 | Cellectis S.A. | Doubly secure transgenic algae or cyanobacteria strains to prevent their establishment and spread in natural ecosystems |
| DK2714936T3 (en) | 2011-06-01 | 2019-03-25 | Prec Biosciences Inc | METHODS AND PRODUCTS FOR PRODUCING MANIPULATED MAMMAL CELL LINES WITH AMPLIFIED TRANSGENES |
| EP2861737B1 (en) | 2012-06-19 | 2019-04-17 | Regents Of The University Of Minnesota | Gene targeting in plants using dna viruses |
| US20140230083A1 (en) | 2012-07-24 | 2014-08-14 | Cellectis | Modular base-specific nucleic acid binding domains from burkholderia rhizoxinica proteins |
| ES2894832T3 (es) | 2012-11-01 | 2022-02-16 | Cellectis | Plantas para la producción de proteínas terapéuticas |
| EP2920310A2 (en) | 2012-11-16 | 2015-09-23 | Total Marketing Services | Method for targeted modification of algae genomes |
| ES2778033T3 (es) | 2012-11-16 | 2020-08-07 | Poseida Therapeutics Inc | Enzimas específicas de sitio y métodos de uso |
| EP2934097B1 (en) | 2012-12-21 | 2018-05-02 | Cellectis | Potatoes with reduced cold-induced sweetening |
| WO2014102688A1 (en) | 2012-12-27 | 2014-07-03 | Cellectis | New design matrix for improvement of homology-directed gene targeting |
| US10113162B2 (en) | 2013-03-15 | 2018-10-30 | Cellectis | Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes |
| MX2016007797A (es) | 2013-12-19 | 2016-09-07 | Amyris Inc | Metodos para integracion genomica. |
| WO2015164748A1 (en) | 2014-04-24 | 2015-10-29 | Sangamo Biosciences, Inc. | Engineered transcription activator like effector (tale) proteins |
| CA2952906A1 (en) | 2014-06-20 | 2015-12-23 | Cellectis | Potatoes with reduced granule-bound starch synthase |
| EP3929286A1 (en) | 2015-06-17 | 2021-12-29 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
| US10837024B2 (en) | 2015-09-17 | 2020-11-17 | Cellectis | Modifying messenger RNA stability in plant transformations |
| EP3410843B1 (en) | 2016-02-02 | 2025-06-18 | Cellectis | Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes |
| WO2017189893A1 (en) | 2016-04-27 | 2017-11-02 | University Of Puerto Rico | 1,5-disubstituted 1,2,3-triazoles are inhibitors of rac/cdc42 gtpases |
| DK3452583T3 (da) | 2016-05-03 | 2022-01-10 | Prec Biosciences Inc | Konstruerede nukleaser, der er nyttige til behandling af hæmofili a |
| EP4219731A3 (en) | 2016-05-18 | 2023-08-09 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| JP2019525759A (ja) * | 2016-07-25 | 2019-09-12 | ブルーバード バイオ， インコーポレイテッド | Ｂｃｌ１１ａホーミングエンドヌクレアーゼバリアント、組成物、および使用方法 |
| UY37482A (es) | 2016-11-16 | 2018-05-31 | Cellectis | Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco |
| US11479782B2 (en) | 2017-04-25 | 2022-10-25 | Cellectis | Alfalfa with reduced lignin composition |
| IL313037A (en) | 2017-05-25 | 2024-07-01 | 2Seventy Bio Inc | Cblb endonuclease variants, compositions, and methods of use |
| US20200299658A1 (en) | 2017-11-01 | 2020-09-24 | Precision Biosciences, Inc. | Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a |
| WO2019126578A1 (en) | 2017-12-20 | 2019-06-27 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
| WO2019126558A1 (en) * | 2017-12-20 | 2019-06-27 | Bluebird Bio, Inc. | Ahr homing endonuclease variants, compositions, and methods of use |
| JP2021527409A (ja) | 2018-06-14 | 2021-10-14 | ブルーバード バイオ， インコーポレイテッド | Ｃｄ７９ａキメラ抗原受容体 |
| WO2020072059A1 (en) | 2018-10-04 | 2020-04-09 | Bluebird Bio, Inc. | Cblb endonuclease variants, compositions, and methods of use |
| NL2022714B1 (en) | 2019-03-11 | 2020-09-18 | Academisch Ziekenhuis Leiden | Optimised RAG1 deficient SCID Gene Therapy |
| WO2024236547A1 (en) | 2023-05-18 | 2024-11-21 | Inceptor Bio, Llc | Modified phagocytic cells expressing chimeric antigen receptors comprising a herpes virus entry mediator (hvem) co-stimulatory domain and uses thereof |
| WO2025046513A1 (en) | 2023-08-29 | 2025-03-06 | Inceptor Bio, Llc | Methods of manufacturing myeloid-derived cells from hematopoietic stem cells and compositions and uses thereof |
| WO2025101946A1 (en) * | 2023-11-08 | 2025-05-15 | Precision Biosciences, Inc. | Polypeptide linkers for use in engineered meganucleases |
| WO2026047626A1 (en) | 2024-08-30 | 2026-03-05 | Inceptor Bio, Llc | Compositions and methods involving immune cells engineered for binding bispecific antibodies for use in cell therapy |
Family Cites Families (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4179337A (en) * | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
| US5006333A (en) * | 1987-08-03 | 1991-04-09 | Ddi Pharmaceuticals, Inc. | Conjugates of superoxide dismutase coupled to high molecular weight polyalkylene glycols |
| FR2646438B1 (fr) * | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
| US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| AU8081491A (en) | 1990-06-01 | 1991-12-31 | Cetus Corporation | Compositions and methods for identifying biologically active molecules |
| US5723286A (en) | 1990-06-20 | 1998-03-03 | Affymax Technologies N.V. | Peptide library and screening systems |
| US5843701A (en) * | 1990-08-02 | 1998-12-01 | Nexstar Pharmaceticals, Inc. | Systematic polypeptide evolution by reverse translation |
| US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
| US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| US6063630A (en) * | 1991-11-05 | 2000-05-16 | Transkaryotic Therapies, Inc. | Targeted introduction of DNA into primary or secondary cells and their use for gene therapy |
| US5436150A (en) * | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
| US20040019916A1 (en) * | 1992-04-24 | 2004-01-29 | Zarling David A. | In vivo homologous sequence targeting in eukaryotic cells |
| US5474896A (en) * | 1992-05-05 | 1995-12-12 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
| US6395959B1 (en) * | 1992-05-05 | 2002-05-28 | Institut Pasteur | Nucleotide sequence encoding the enzyme I SceI and the use thereof |
| US5792632A (en) * | 1992-05-05 | 1998-08-11 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
| ATE239785T1 (de) | 1993-02-12 | 2003-05-15 | Univ Johns Hopkins | Funktionelle domänen der restriktionsendonukleasen aus -i(flavobakterium okeanokoites)(foki) |
| US5801030A (en) * | 1995-09-01 | 1998-09-01 | Genvec, Inc. | Methods and vectors for site-specific recombination |
| CA2232727C (en) * | 1995-09-22 | 2002-03-26 | Novo Nordisk A/S | Novel variants of green fluorescent protein, gfp |
| US5830729A (en) * | 1996-04-18 | 1998-11-03 | Institut Pasteur | I Sce I-induced gene replacement and gene conversion in embryonic stem cells |
| US6261804B1 (en) | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
| IL120241A0 (en) * | 1997-02-17 | 1997-06-10 | B G Negev Technologies And App | DNA segments encoding a domain of ho-endonuclease |
| US6232112B1 (en) * | 1997-11-24 | 2001-05-15 | Flinders Technologies Pty Ltd Of Flinders University | Reagents and methods for diversification of DNA |
| JP2002535994A (ja) | 1999-02-03 | 2002-10-29 | ザ チルドレンズ メディカル センター コーポレイション | 標的ｄｎａのインビボ除去による遺伝子修復 |
| WO2000046386A2 (en) | 1999-02-03 | 2000-08-10 | The Children's Medical Center Corporation | Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site |
| AU2001247676A1 (en) | 2000-03-21 | 2001-10-03 | Neugenesis Corporation | Methods for in vivo diversification of single genes |
| CA2429971A1 (en) | 2000-11-27 | 2002-05-30 | Sloan-Kettering Institute For Cancer Research | Methods using fluorescens energy transfer probes for detecting cleavage of nucleic acids |
| US20030096249A1 (en) * | 2000-12-21 | 2003-05-22 | Westphal Ryan S. | Nucleic acid molecules and polypeptides for a human cation channel polypeptide |
| WO2002099105A2 (en) | 2001-06-05 | 2002-12-12 | Cellectis | Methods for modifying the cpg content of polynucleotides |
| DE10131786A1 (de) * | 2001-07-04 | 2003-01-16 | Sungene Gmbh & Co Kgaa | Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen |
| US20030228583A1 (en) * | 2001-10-31 | 2003-12-11 | Amacher David E. | Biomarkers of liver response |
| EP1458875A2 (de) * | 2001-12-20 | 2004-09-22 | Sungene GmbH & Co. KGaA | Verfahren zur transformation von pflanzlichen plastiden |
| US20060078552A1 (en) * | 2002-03-15 | 2006-04-13 | Sylvain Arnould | Hybrid and single chain meganucleases and use thereof |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| US20060153826A1 (en) * | 2003-01-28 | 2006-07-13 | Sylvain Arnould | Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof |
| US7888121B2 (en) * | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| WO2006097784A1 (en) * | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| EP2327771A1 (en) | 2005-03-15 | 2011-06-01 | Cellectis | I-crel meganuclease variants with modified specificity, method of preparation and uses thereof |
| WO2007034262A1 (en) | 2005-09-19 | 2007-03-29 | Cellectis | Heterodimeric meganucleases and use thereof |
| CA2891996A1 (en) * | 2005-10-18 | 2007-04-26 | Duke University | Rationally-designed meganucleases with altered sequence specificity and dna-binding affinity |
| WO2007049095A1 (en) | 2005-10-25 | 2007-05-03 | Cellectis | Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| WO2007093836A1 (en) * | 2006-02-13 | 2007-08-23 | Cellectis | Meganuclease variants cleaving a dna target sequence from a xp gene and uses thereof |
| WO2008002199A2 (en) | 2006-06-23 | 2008-01-03 | Aichinger, Elena | Biocidal and virulicidal agent-clathrate 1,6,3,8-dimethan-tetra-aza-cyclodecane-sodium carbonate |
| RU2318466C1 (ru) | 2006-06-23 | 2008-03-10 | Давид Георгиевич Кочиев | Лазерная установка для абляции тканей и литотрипсии |
| WO2008002274A1 (en) | 2006-06-27 | 2008-01-03 | Inventys Pte Ltd | Method and system for client-side user interface enhancement to enable application integration and portalisation |
| WO2008010009A1 (en) | 2006-07-18 | 2008-01-24 | Cellectis | Meganuclease variants cleaving a dna target sequence from a rag gene and uses thereof |
| EP1884516A1 (en) | 2006-08-04 | 2008-02-06 | Ferrer Internacional, S.A. | Pyrazolo[1,5-a]pyrimidines, processes, uses and compositions |
| WO2008059317A1 (en) | 2006-11-14 | 2008-05-22 | Cellectis | Meganuclease variants cleaving a dna target sequence from the hprt gene and uses thereof |
| AU2007320880A1 (en) | 2006-11-14 | 2008-05-22 | Cellectis | Meganuclease variants cleaving a DNA target sequence from the HPRT gene and uses thereof |
| WO2008093152A1 (en) | 2007-02-01 | 2008-08-07 | Cellectis | Obligate heterodimer meganucleases and uses thereof |
| WO2008149176A1 (en) | 2007-06-06 | 2008-12-11 | Cellectis | Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof |
| CN101784658A (zh) | 2007-06-06 | 2010-07-21 | 赛莱克蒂斯公司 | 增强来自I-CreI的大范围核酸酶的切割活性的方法 |
| EP2215223B1 (en) | 2007-10-31 | 2013-05-01 | Precision Biosciences, Inc. | Rationally-designed single-chain meganucleases with non-palindromic recognition sequences |
| JP2012501641A (ja) | 2008-09-08 | 2012-01-26 | セレクティス | グルタミンシンセターゼ遺伝子からのｄｎａ標的配列を切断するメガヌクレアーゼ変異型及びその使用 |
-
2008
- 2008-01-31 WO PCT/IB2008/001331 patent/WO2009095742A1/en not_active Ceased
-
2009
- 2009-02-02 AU AU2009208732A patent/AU2009208732A1/en not_active Abandoned
- 2009-02-02 CN CN200980109080.1A patent/CN101970649B/zh not_active Expired - Fee Related
- 2009-02-02 EP EP09705925A patent/EP2245147A1/en not_active Withdrawn
- 2009-02-02 US US12/864,998 patent/US8927247B2/en not_active Expired - Fee Related
- 2009-02-02 JP JP2010544817A patent/JP2011514144A/ja active Pending
- 2009-02-02 WO PCT/IB2009/000439 patent/WO2009095793A1/en not_active Ceased
- 2009-02-02 CA CA2729124A patent/CA2729124A1/en not_active Abandoned
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