JP2010532668A5 - - Google Patents

Info

Publication number

JP2010532668A5

JP2010532668A5 JP2010516161A JP2010516161A JP2010532668A5 JP 2010532668 A5 JP2010532668 A5 JP 2010532668A5 JP 2010516161 A JP2010516161 A JP 2010516161A JP 2010516161 A JP2010516161 A JP 2010516161A JP 2010532668 A5 JP2010532668 A5 JP 2010532668A5

Authority

JP

Japan

Prior art keywords

mipol1

detecting

gene

amplification

etv1

Prior art date

2008-07-03

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 238000003199 nucleic acid amplification method Methods 0.000 claims description 26
• 238000000034 method Methods 0.000 claims description 23
• 230000003321 amplification Effects 0.000 claims description 22
• 239000000523 sample Substances 0.000 claims description 19
• 108091034117 Oligonucleotide Proteins 0.000 claims description 13
• 108090000623 proteins and genes Proteins 0.000 claims description 13
• 230000008707 rearrangement Effects 0.000 claims description 13
• 108020004414 DNA Proteins 0.000 claims description 10
• 230000008711 chromosomal rearrangement Effects 0.000 claims description 10
• 239000000203 mixture Substances 0.000 claims description 10
• 101150015184 Etv1 gene Proteins 0.000 claims description 9
• 101100457323 Homo sapiens MIPOL1 gene Proteins 0.000 claims description 9
• 101150020920 MIPOL1 gene Proteins 0.000 claims description 9
• 206010060862 Prostate cancer Diseases 0.000 claims description 9
• 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 9
• 210000002700 urine Anatomy 0.000 claims description 6
• 239000000047 product Substances 0.000 claims description 5
• 238000007901 in situ hybridization Methods 0.000 claims description 4
• 238000007834 ligase chain reaction Methods 0.000 claims description 4
• 238000007899 nucleic acid hybridization Methods 0.000 claims description 4
• 150000007523 nucleic acids Chemical class 0.000 claims description 4
• 238000003752 polymerase chain reaction Methods 0.000 claims description 4
• 238000003757 reverse transcription PCR Methods 0.000 claims description 4
• 102100039563 ETS translocation variant 1 Human genes 0.000 claims description 2
• 101000813729 Homo sapiens ETS translocation variant 1 Proteins 0.000 claims description 2
• 101000628946 Homo sapiens Mirror-image polydactyly gene 1 protein Proteins 0.000 claims description 2
• 102100026928 Mirror-image polydactyly gene 1 protein Human genes 0.000 claims description 2
• 238000002105 Southern blotting Methods 0.000 claims description 2
• 238000004458 analytical method Methods 0.000 claims description 2
• 239000012472 biological sample Substances 0.000 claims description 2
• 210000004369 blood Anatomy 0.000 claims description 2
• 239000008280 blood Substances 0.000 claims description 2
• 238000006073 displacement reaction Methods 0.000 claims description 2
• 238000009396 hybridization Methods 0.000 claims description 2
• 238000009830 intercalation Methods 0.000 claims description 2
• 230000001404 mediated effect Effects 0.000 claims description 2
• 238000010208 microarray analysis Methods 0.000 claims description 2
• 108020004707 nucleic acids Proteins 0.000 claims description 2
• 102000039446 nucleic acids Human genes 0.000 claims description 2
• 239000008188 pellet Substances 0.000 claims description 2
• 210000002381 plasma Anatomy 0.000 claims description 2
• 210000002307 prostate Anatomy 0.000 claims description 2
• 210000005267 prostate cell Anatomy 0.000 claims description 2
• 230000028327 secretion Effects 0.000 claims description 2
• 210000000582 semen Anatomy 0.000 claims description 2
• 238000012163 sequencing technique Methods 0.000 claims description 2
• 210000002966 serum Anatomy 0.000 claims description 2
• 239000006228 supernatant Substances 0.000 claims description 2
• 210000001519 tissue Anatomy 0.000 claims description 2
• 238000013518 transcription Methods 0.000 claims description 2
• 230000035897 transcription Effects 0.000 claims description 2
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
• 238000002372 labelling Methods 0.000 description 1
• 239000000126 substance Substances 0.000 description 1