JP2010516232A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010516232A5 JP2010516232A5 JP2009545787A JP2009545787A JP2010516232A5 JP 2010516232 A5 JP2010516232 A5 JP 2010516232A5 JP 2009545787 A JP2009545787 A JP 2009545787A JP 2009545787 A JP2009545787 A JP 2009545787A JP 2010516232 A5 JP2010516232 A5 JP 2010516232A5
- Authority
- JP
- Japan
- Prior art keywords
- csr
- chimeric protein
- protein
- chimeric
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000037865 fusion proteins Human genes 0.000 claims 32
- 108020001507 fusion proteins Proteins 0.000 claims 32
- 238000000034 method Methods 0.000 claims 23
- 210000004027 cell Anatomy 0.000 claims 20
- 101000659767 Homo sapiens Taste receptor type 1 member 1 Proteins 0.000 claims 13
- 102100035941 Taste receptor type 1 member 1 Human genes 0.000 claims 13
- 101000659774 Homo sapiens Taste receptor type 1 member 3 Proteins 0.000 claims 10
- 102100035942 Taste receptor type 1 member 3 Human genes 0.000 claims 10
- 239000003795 chemical substances by application Substances 0.000 claims 10
- 235000019583 umami taste Nutrition 0.000 claims 10
- 108091006027 G proteins Proteins 0.000 claims 9
- 102000030782 GTP binding Human genes 0.000 claims 9
- 108091000058 GTP-Binding Proteins 0.000 claims 9
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 9
- 239000003446 ligand Substances 0.000 claims 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 5
- 229910052791 calcium Inorganic materials 0.000 claims 5
- 239000011575 calcium Substances 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 239000000556 agonist Substances 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- 230000003834 intracellular effect Effects 0.000 claims 3
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 2
- 102100039215 Guanine nucleotide-binding protein G(t) subunit alpha-3 Human genes 0.000 claims 2
- 239000005557 antagonist Substances 0.000 claims 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims 2
- 239000001110 calcium chloride Substances 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 108010005995 gustducin Proteins 0.000 claims 2
- 239000000833 heterodimer Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- -1 inverse agonist Substances 0.000 claims 2
- 229940125425 inverse agonist Drugs 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000004031 partial agonist Substances 0.000 claims 2
- 102000005962 receptors Human genes 0.000 claims 2
- 108020003175 receptors Proteins 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 claims 1
- 239000003012 bilayer membrane Substances 0.000 claims 1
- 238000002306 biochemical method Methods 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000003623 enhancer Substances 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 238000001506 fluorescence spectroscopy Methods 0.000 claims 1
- 244000000013 helminth Species 0.000 claims 1
- 239000013642 negative control Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000011664 signaling Effects 0.000 claims 1
- 239000007790 solid phase Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 235000019640 taste Nutrition 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88098107P | 2007-01-18 | 2007-01-18 | |
| US60/880,981 | 2007-01-18 | ||
| PCT/CH2007/000636 WO2008086634A2 (en) | 2007-01-18 | 2007-12-14 | Chimeric umami taste receptor, nucleic acid encoding it and use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010516232A JP2010516232A (ja) | 2010-05-20 |
| JP2010516232A5 true JP2010516232A5 (enExample) | 2012-12-27 |
| JP5301464B2 JP5301464B2 (ja) | 2013-09-25 |
Family
ID=39521493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009545787A Active JP5301464B2 (ja) | 2007-01-18 | 2007-12-14 | キメラうま味受容体、それをコードする核酸およびその利用 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8039233B2 (enExample) |
| EP (1) | EP2121748B1 (enExample) |
| JP (1) | JP5301464B2 (enExample) |
| KR (1) | KR101511394B1 (enExample) |
| WO (1) | WO2008086634A2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198836C (zh) * | 1998-07-28 | 2005-04-27 | 加利福尼亚大学董事会 | 编码与感觉转导有关的g－蛋白偶联受体的核酸 |
| CN102369275A (zh) * | 2009-02-02 | 2012-03-07 | 卓莫赛尔公司 | 新型细胞系和方法 |
| WO2013072332A1 (en) | 2011-11-14 | 2013-05-23 | Givaudan Sa | Methods of using antagonists of bitter taste receptors |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5825980B2 (ja) | 1976-02-12 | 1983-05-31 | ヤマサ醤油株式会社 | サイクリックヌクレオチドの定量法 |
| CA2003345A1 (en) | 1988-11-18 | 1990-05-18 | Joel F. Habener | Camp-responsive transcriptional enhancer binding protein |
| US5462856A (en) | 1990-07-19 | 1995-10-31 | Bunsen Rush Laboratories, Inc. | Methods for identifying chemicals that act as agonists or antagonists for receptors and other proteins involved in signal transduction via pathways that utilize G-proteins |
| US5401629A (en) | 1990-08-07 | 1995-03-28 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Assay methods and compositions useful for measuring the transduction of an intracellular signal |
| JP4339542B2 (ja) | 1999-09-10 | 2009-10-07 | ザ、リージェンツ、オブ、ザ、ユニバーシティ、オブ、カリフォルニア | Ｔ２ｒ、すなわち味覚受容体の新規ファミリー |
| TW201022287A (en) | 2001-01-03 | 2010-06-16 | Senomyx Inc | T1R taste receptors and genes encoding same |
| US7368285B2 (en) | 2001-03-07 | 2008-05-06 | Senomyx, Inc. | Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same |
| IL159243A0 (en) * | 2001-06-26 | 2004-06-01 | Senomyx Inc | Tir hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds |
| EP1585954B1 (en) * | 2001-07-03 | 2014-03-26 | The Regents of The University of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| WO2004055048A2 (en) | 2002-12-18 | 2004-07-01 | Givaudan Sa | Chimeric alpha q-gustducin g-proteins |
| CN103275058B (zh) | 2003-08-06 | 2016-02-24 | 西诺米克斯公司 | 调味剂、味道调节剂、促味剂、味觉增强剂、鲜味剂或甜味剂和/或增强剂及其用途 |
| US20060045953A1 (en) | 2004-08-06 | 2006-03-02 | Catherine Tachdjian | Aromatic amides and ureas and their uses as sweet and/or umami flavor modifiers, tastants and taste enhancers |
| EP2035827B1 (en) * | 2006-06-19 | 2012-10-17 | Givaudan S.A. | Nucleic acid, polypeptide and its use |
-
2007
- 2007-12-14 KR KR1020097017081A patent/KR101511394B1/ko active Active
- 2007-12-14 WO PCT/CH2007/000636 patent/WO2008086634A2/en not_active Ceased
- 2007-12-14 US US12/523,669 patent/US8039233B2/en active Active
- 2007-12-14 JP JP2009545787A patent/JP5301464B2/ja active Active
- 2007-12-14 EP EP07845633.2A patent/EP2121748B1/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2009540804A5 (enExample) | ||
| Koval et al. | Mix and match: investigating heteromeric and heterotypic gap junction channels in model systems and native tissues | |
| Navarro et al. | Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs | |
| Bakker et al. | Discovery of naturally occurring splice variants of the rat histamine H3 receptor that act as dominant-negative isoforms | |
| JP2009534018A5 (enExample) | ||
| BR112019018196A2 (pt) | método para identificar compostos de modulação de mau odor | |
| JP2004513608A5 (enExample) | ||
| De-la-Rosa et al. | Coarse architecture of the transient receptor potential vanilloid 1 (TRPV1) ion channel determined by fluorescence resonance energy transfer | |
| JP2009533065A5 (enExample) | ||
| Harikumar et al. | Pattern of intra-family hetero-oligomerization involving the G-protein-coupled secretin receptor | |
| JP6251252B2 (ja) | アッセイ | |
| Zheng et al. | Analysis of the interactions between the C-terminal cytoplasmic domains of KCNQ1 and KCNE1 channel subunits | |
| CA2929392A1 (en) | Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through camp pathways | |
| Swan et al. | Differential effects of mutations of POPDC proteins on heteromeric interaction and membrane trafficking | |
| JP2019037197A (ja) | ロースト様香料素材のスクリーニング方法 | |
| JP2010516232A5 (enExample) | ||
| Xu et al. | The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands | |
| JP2019129772A (ja) | ２−ヘプタノンの匂いを抑制する物質のスクリーニング方法 | |
| Ma et al. | Movement of hClC-1 C-termini during common gating and limits on their cytoplasmic location | |
| Vitko et al. | Orientation of the calcium channel β relative to the α12. 2 subunit is critical for its regulation of channel activity | |
| JP2019010058A (ja) | フェノリック香料素材のスクリーニング方法 | |
| Ashikawa et al. | GFP‐based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs | |
| JP2019129773A (ja) | ２−ノナノンの匂いを抑制する物質のスクリーニング方法 | |
| US20190277845A1 (en) | Screening assays to identify compounds which modulate t1r associated taste modalities which eliminate false positives | |
| Skafar et al. | Understanding the human estrogen receptor-alpha using targeted mutagenesis |