JP2010516232A5 - - Google Patents
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- Publication number
- JP2010516232A5 JP2010516232A5 JP2009545787A JP2009545787A JP2010516232A5 JP 2010516232 A5 JP2010516232 A5 JP 2010516232A5 JP 2009545787 A JP2009545787 A JP 2009545787A JP 2009545787 A JP2009545787 A JP 2009545787A JP 2010516232 A5 JP2010516232 A5 JP 2010516232A5
- Authority
- JP
- Japan
- Prior art keywords
- csr
- chimeric protein
- protein
- chimeric
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000037865 fusion proteins Human genes 0.000 claims 32
- 108020001507 fusion proteins Proteins 0.000 claims 32
- 238000000034 method Methods 0.000 claims 23
- 210000004027 cell Anatomy 0.000 claims 20
- 101000659767 Homo sapiens Taste receptor type 1 member 1 Proteins 0.000 claims 13
- 102100035941 Taste receptor type 1 member 1 Human genes 0.000 claims 13
- 101000659774 Homo sapiens Taste receptor type 1 member 3 Proteins 0.000 claims 10
- 102100035942 Taste receptor type 1 member 3 Human genes 0.000 claims 10
- 239000003795 chemical substances by application Substances 0.000 claims 10
- 235000019583 umami taste Nutrition 0.000 claims 10
- 108091006027 G proteins Proteins 0.000 claims 9
- 102000030782 GTP binding Human genes 0.000 claims 9
- 108091000058 GTP-Binding Proteins 0.000 claims 9
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 9
- 239000003446 ligand Substances 0.000 claims 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 5
- 229910052791 calcium Inorganic materials 0.000 claims 5
- 239000011575 calcium Substances 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 239000000556 agonist Substances 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- 230000003834 intracellular effect Effects 0.000 claims 3
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 2
- 102100039215 Guanine nucleotide-binding protein G(t) subunit alpha-3 Human genes 0.000 claims 2
- 239000005557 antagonist Substances 0.000 claims 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims 2
- 239000001110 calcium chloride Substances 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 108010005995 gustducin Proteins 0.000 claims 2
- 239000000833 heterodimer Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- -1 inverse agonist Substances 0.000 claims 2
- 229940125425 inverse agonist Drugs 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000004031 partial agonist Substances 0.000 claims 2
- 102000005962 receptors Human genes 0.000 claims 2
- 108020003175 receptors Proteins 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 claims 1
- 239000003012 bilayer membrane Substances 0.000 claims 1
- 238000002306 biochemical method Methods 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000003623 enhancer Substances 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 238000001506 fluorescence spectroscopy Methods 0.000 claims 1
- 244000000013 helminth Species 0.000 claims 1
- 239000013642 negative control Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000011664 signaling Effects 0.000 claims 1
- 239000007790 solid phase Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 235000019640 taste Nutrition 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88098107P | 2007-01-18 | 2007-01-18 | |
| US60/880,981 | 2007-01-18 | ||
| PCT/CH2007/000636 WO2008086634A2 (en) | 2007-01-18 | 2007-12-14 | Chimeric umami taste receptor, nucleic acid encoding it and use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010516232A JP2010516232A (ja) | 2010-05-20 |
| JP2010516232A5 true JP2010516232A5 (enExample) | 2012-12-27 |
| JP5301464B2 JP5301464B2 (ja) | 2013-09-25 |
Family
ID=39521493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009545787A Active JP5301464B2 (ja) | 2007-01-18 | 2007-12-14 | キメラうま味受容体、それをコードする核酸およびその利用 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8039233B2 (enExample) |
| EP (1) | EP2121748B1 (enExample) |
| JP (1) | JP5301464B2 (enExample) |
| KR (1) | KR101511394B1 (enExample) |
| WO (1) | WO2008086634A2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9912545A (pt) * | 1998-07-28 | 2001-10-30 | Univ California | ácidos nucleicos codificando um receptorcopulado a proteìna g envolvido em transduçãosensorial |
| EP2924112A1 (en) * | 2009-02-02 | 2015-09-30 | Chromocell Corporation | Novel cell lines and methods |
| WO2013072332A1 (en) | 2011-11-14 | 2013-05-23 | Givaudan Sa | Methods of using antagonists of bitter taste receptors |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5825980B2 (ja) * | 1976-02-12 | 1983-05-31 | ヤマサ醤油株式会社 | サイクリックヌクレオチドの定量法 |
| AU4809290A (en) * | 1988-11-18 | 1990-06-12 | General Hospital Corporation, The | A camp-responsive transcriptional enhancer binding protein |
| US5462856A (en) * | 1990-07-19 | 1995-10-31 | Bunsen Rush Laboratories, Inc. | Methods for identifying chemicals that act as agonists or antagonists for receptors and other proteins involved in signal transduction via pathways that utilize G-proteins |
| US5401629A (en) * | 1990-08-07 | 1995-03-28 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Assay methods and compositions useful for measuring the transduction of an intracellular signal |
| EP1214343A2 (en) | 1999-09-10 | 2002-06-19 | The Regents Of The University Of California | T2r taste receptor family |
| TW201022287A (en) * | 2001-01-03 | 2010-06-16 | Senomyx Inc | T1R taste receptors and genes encoding same |
| US7368285B2 (en) * | 2001-03-07 | 2008-05-06 | Senomyx, Inc. | Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same |
| CN108329393A (zh) * | 2001-06-26 | 2018-07-27 | 塞诺米克斯公司 | T1r异源寡聚的味觉受体和表达所述受体的细胞系及其在鉴定味觉化合物中的用途 |
| WO2003004992A2 (en) * | 2001-07-03 | 2003-01-16 | The Regents Of The University Of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| AU2003286069A1 (en) * | 2002-12-18 | 2004-07-09 | Givaudan Sa | Chimeric alpha q-gustducin g-proteins |
| AR046078A1 (es) | 2003-08-06 | 2005-11-23 | Senomyx Inc | Nuevos sabores, modificadores de sabores, sustancias gustativas, mejoradores de gusto, sustancias gustativas y/o mejoradoras del gusto umani o dulce y uso de los mismos. |
| US20060045953A1 (en) * | 2004-08-06 | 2006-03-02 | Catherine Tachdjian | Aromatic amides and ureas and their uses as sweet and/or umami flavor modifiers, tastants and taste enhancers |
| US7964376B2 (en) * | 2006-06-19 | 2011-06-21 | Givaudan Sa | Chimeric proteins and their use in methods to screen for sweet taste modulators |
-
2007
- 2007-12-14 US US12/523,669 patent/US8039233B2/en active Active
- 2007-12-14 EP EP07845633.2A patent/EP2121748B1/en active Active
- 2007-12-14 JP JP2009545787A patent/JP5301464B2/ja active Active
- 2007-12-14 KR KR1020097017081A patent/KR101511394B1/ko active Active
- 2007-12-14 WO PCT/CH2007/000636 patent/WO2008086634A2/en not_active Ceased
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