JP2009540804A5 - - Google Patents
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- Publication number
- JP2009540804A5 JP2009540804A5 JP2009515685A JP2009515685A JP2009540804A5 JP 2009540804 A5 JP2009540804 A5 JP 2009540804A5 JP 2009515685 A JP2009515685 A JP 2009515685A JP 2009515685 A JP2009515685 A JP 2009515685A JP 2009540804 A5 JP2009540804 A5 JP 2009540804A5
- Authority
- JP
- Japan
- Prior art keywords
- csr
- protein
- chimeric protein
- seq
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000037865 fusion proteins Human genes 0.000 claims 27
- 108020001507 fusion proteins Proteins 0.000 claims 27
- 210000004027 cell Anatomy 0.000 claims 24
- 238000000034 method Methods 0.000 claims 20
- 239000003795 chemical substances by application Substances 0.000 claims 10
- 108091006027 G proteins Proteins 0.000 claims 9
- 102000030782 GTP binding Human genes 0.000 claims 9
- 108091000058 GTP-Binding Proteins 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 101000659774 Homo sapiens Taste receptor type 1 member 3 Proteins 0.000 claims 7
- 102100035942 Taste receptor type 1 member 3 Human genes 0.000 claims 7
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 229920001184 polypeptide Polymers 0.000 claims 7
- 102000004196 processed proteins & peptides Human genes 0.000 claims 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims 7
- 101000659765 Homo sapiens Taste receptor type 1 member 2 Proteins 0.000 claims 6
- 102100035948 Taste receptor type 1 member 2 Human genes 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 5
- 229910052791 calcium Inorganic materials 0.000 claims 5
- 239000011575 calcium Substances 0.000 claims 5
- 239000003446 ligand Substances 0.000 claims 5
- 210000004962 mammalian cell Anatomy 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 2
- 102100039215 Guanine nucleotide-binding protein G(t) subunit alpha-3 Human genes 0.000 claims 2
- 241000238631 Hexapoda Species 0.000 claims 2
- 239000000556 agonist Substances 0.000 claims 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims 2
- 239000001110 calcium chloride Substances 0.000 claims 2
- 239000003623 enhancer Substances 0.000 claims 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims 2
- 235000003599 food sweetener Nutrition 0.000 claims 2
- 108010005995 gustducin Proteins 0.000 claims 2
- 244000000013 helminth Species 0.000 claims 2
- 239000000833 heterodimer Substances 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000003834 intracellular effect Effects 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000003765 sweetening agent Substances 0.000 claims 2
- 210000005253 yeast cell Anatomy 0.000 claims 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 1
- 108010034145 Helminth Proteins Proteins 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 238000005481 NMR spectroscopy Methods 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 claims 1
- 238000002306 biochemical method Methods 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 238000001506 fluorescence spectroscopy Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 239000013642 negative control Substances 0.000 claims 1
- 239000007790 solid phase Substances 0.000 claims 1
- 235000019605 sweet taste sensations Nutrition 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81486606P | 2006-06-19 | 2006-06-19 | |
| US60/814,866 | 2006-06-19 | ||
| US88140207P | 2007-01-19 | 2007-01-19 | |
| US60/881,402 | 2007-01-19 | ||
| PCT/CH2007/000297 WO2007147275A1 (en) | 2006-06-19 | 2007-06-15 | Nucleic acid, polypeptide and its use |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009540804A JP2009540804A (ja) | 2009-11-26 |
| JP2009540804A5 true JP2009540804A5 (enExample) | 2012-06-07 |
| JP5225986B2 JP5225986B2 (ja) | 2013-07-03 |
Family
ID=38608811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009515685A Active JP5225986B2 (ja) | 2006-06-19 | 2007-06-15 | 核酸、ポリペプチドおよびその利用 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7964376B2 (enExample) |
| EP (1) | EP2035827B1 (enExample) |
| JP (1) | JP5225986B2 (enExample) |
| KR (1) | KR101440639B1 (enExample) |
| WO (1) | WO2007147275A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101511394B1 (ko) * | 2007-01-18 | 2015-04-10 | 지보당 에스아 | 키메라 우마미 미각 수용체, 이를 코딩하는 핵산 및 이의 용도 |
| BR122021005297B1 (pt) | 2010-06-02 | 2022-02-22 | Evolva, Inc | Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol |
| JP6262653B2 (ja) | 2011-08-08 | 2018-01-17 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | ステビオール配糖体の組換え生産 |
| WO2013072332A1 (en) | 2011-11-14 | 2013-05-23 | Givaudan Sa | Methods of using antagonists of bitter taste receptors |
| KR20150115002A (ko) | 2013-02-06 | 2015-10-13 | 에볼바 에스아 | 레바우디오시드 ｄ 및 레바우디오시드 ｍ의 개선된 생산 방법 |
| CN105189771B (zh) | 2013-02-11 | 2019-11-05 | 埃沃尔瓦公司 | 在重组宿主中有效产生甜菊醇糖苷 |
| MX384586B (es) | 2013-05-10 | 2025-03-14 | Pepsico Inc | Ensayo de internalización del receptor del gusto. |
| US10107794B2 (en) | 2013-05-10 | 2018-10-23 | Pepsico, Inc. | Methods of using U2-OS cells to identify potential sweet taste modulators |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| US10612064B2 (en) | 2014-09-09 | 2020-04-07 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP4148137A1 (en) | 2015-01-30 | 2023-03-15 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| CA2979931A1 (en) | 2015-03-16 | 2016-09-22 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| BR112018002564A2 (pt) | 2015-08-07 | 2018-09-25 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| CN109195457A (zh) | 2016-04-13 | 2019-01-11 | 埃沃尔瓦公司 | 在重组宿主中产生甜菊醇糖苷 |
| US10815514B2 (en) | 2016-05-16 | 2020-10-27 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CN110100006A (zh) | 2016-11-07 | 2019-08-06 | 埃沃尔瓦公司 | 重组宿主中甜菊糖苷的生产 |
| KR102624889B1 (ko) * | 2021-11-30 | 2024-01-15 | 서울대학교산학협력단 | 트립토판 형광 분석을 이용한 인간 단맛 수용체의 리간드 결합 도메인의 맛 물질에 대한 리간드 효능 분석 방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002320294B2 (en) * | 2001-07-03 | 2009-01-15 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Mammalian sweet and amino acid heterodimeric taste receptors |
| NZ619746A (en) | 2003-08-06 | 2014-05-30 | Senomyx Inc | Novel flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof |
-
2007
- 2007-06-15 WO PCT/CH2007/000297 patent/WO2007147275A1/en not_active Ceased
- 2007-06-15 KR KR1020087030956A patent/KR101440639B1/ko active Active
- 2007-06-15 JP JP2009515685A patent/JP5225986B2/ja active Active
- 2007-06-15 EP EP07720194A patent/EP2035827B1/en active Active
- 2007-06-15 US US12/305,605 patent/US7964376B2/en active Active
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