JP2010235551A - 美白剤、抗老化剤、プロフィラグリンmRNA発現上昇促進剤、育毛剤及び毛乳頭細胞増殖促進剤 - Google Patents
美白剤、抗老化剤、プロフィラグリンmRNA発現上昇促進剤、育毛剤及び毛乳頭細胞増殖促進剤 Download PDFInfo
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Abstract
【解決手段】本発明の美白剤、抗老化剤、プロフィラグリンmRNA発現上昇促進剤、育毛剤又は毛乳頭細胞増殖促進剤に、琥珀熱水抽出物を含有せしめる。
【選択図】なし
Description
本実施形態の美白剤、抗老化剤、プロフィラグリンmRNA発現上昇促進剤、育毛剤又は毛乳頭細胞増殖促進剤は、琥珀熱水抽出物を有効成分として含有する。
琥珀の粉砕物50gを90℃の熱水1000mL中に加え、2時間抽出し、熱時濾過した。残渣についてさらに同様の抽出処理をした。得られた抽出液を合わせて減圧下で濃縮し、さらに乾燥して琥珀熱水抽出物0.0005gを得た(試料1)。
製品名:Agilent 1100(Agilent Technologies社製)
固定相:Wakosil-II 5C18 HG(和光純薬工業社製)
カラム径:4.6mm
カラム長:150mm
移動相:85%リン酸バッファー
移動相流速:1.0mL/分
検出器:UV
検出波長:210nm
製造例1により得られた琥珀熱水抽出物(試料1)について、以下のようにしてB16メラノーマ細胞に対するメラニン産生抑制作用を試験した。
式中、Aは「試料無添加時の475nmにおける吸光度」を表し、Bは「試料添加時の475nmにおける吸光度」を表し、Cは「試料無添加時の540nmにおける吸光度」を表し、Dは「試料添加時の540nmにおける吸光度」を表す。
結果を表1に示す。
製造例1により得られた琥珀熱水抽出物(試料1)について、以下のようにしてグルタチオン産生促進作用を試験した。
式中、Aは「試料無添加時の細胞中における総タンパク量当たりのグルタチオン量(対照)」を表し、Bは「試料添加時の細胞中における総タンパク量当たりのグルタチオン量」を表す。
結果を表2に示す。
製造例1により得られた琥珀熱水抽出物(試料1)について、以下のようにしてプロフィラグリンmRNA発現上昇促進作用を試験した。
式中Aは「試料添加時の補正値」を表し、Bは「試料無添加時の補正値」を表す。
結果を表3に示す。
製造例1により得られた琥珀熱水抽出物(試料1)について、以下のようにして毛乳頭細胞増殖促進作用を試験した。
なお、式中、Aは「試料添加時の吸光度」を表し、Bは「試料無添加時の吸光度」を表す。
結果を表4に示す。
Claims (6)
- 琥珀熱水抽出物を有効成分として含有することを特徴とする美白剤。
- 前記琥珀熱水抽出物が、メラニン産生抑制作用及び/又はグルタチオン産生促進作用を有することを特徴とする請求項1に記載の美白剤。
- 琥珀熱水抽出物を有効成分として含有することを特徴とする抗老化剤。
- 琥珀熱水抽出物を有効成分として含有することを特徴とするプロフィラグリンmRNA発現上昇促進剤。
- 琥珀熱水抽出物を有効成分として含有することを特徴とする育毛剤。
- 琥珀熱水抽出物を有効成分として含有することを特徴とする毛乳頭細胞増殖促進剤。
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JP2012240967A (ja) * | 2011-05-19 | 2012-12-10 | Institute Of Physical & Chemical Research | 琥珀から得られるエンドセリン−1産生抑制剤、scf産生抑制剤、及びメラニン産生抑制剤並びにその使用 |
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JP2015160821A (ja) * | 2014-02-26 | 2015-09-07 | 共栄化学工業株式会社 | 美白用組成物及び化粧料 |
WO2017119143A1 (ja) * | 2016-01-06 | 2017-07-13 | 株式会社琥珀研究所byヤマノ | 皮膚外用剤 |
WO2018212362A1 (ja) | 2017-05-19 | 2018-11-22 | 株式会社ヤマノビューティケミカル | 糖質の分解・吸収抑制剤 |
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JP2019194169A (ja) * | 2018-05-02 | 2019-11-07 | 国立大学法人岩手大学 | メラニン産生抑制剤 |
WO2020031638A1 (ja) | 2018-08-10 | 2020-02-13 | 琥珀バイオテクノロジー株式会社 | アンジオテンシンi変換酵素活性阻害剤 |
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