JP2009530378A5 - - Google Patents
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- Publication number
- JP2009530378A5 JP2009530378A5 JP2009501443A JP2009501443A JP2009530378A5 JP 2009530378 A5 JP2009530378 A5 JP 2009530378A5 JP 2009501443 A JP2009501443 A JP 2009501443A JP 2009501443 A JP2009501443 A JP 2009501443A JP 2009530378 A5 JP2009530378 A5 JP 2009530378A5
- Authority
- JP
- Japan
- Prior art keywords
- exchange chromatography
- mixture
- target protein
- deae
- steps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 claims 15
- 102000004169 proteins and genes Human genes 0.000 claims 14
- 108090000623 proteins and genes Proteins 0.000 claims 14
- 239000000203 mixture Substances 0.000 claims 9
- 238000005571 anion exchange chromatography Methods 0.000 claims 6
- -1 sulfoisobutyl Chemical group 0.000 claims 6
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims 5
- 238000005277 cation exchange chromatography Methods 0.000 claims 5
- 239000000919 ceramic Substances 0.000 claims 5
- 239000000356 contaminant Substances 0.000 claims 5
- 229920001467 poly(styrenesulfonates) Polymers 0.000 claims 5
- 229920005989 resin Polymers 0.000 claims 5
- 239000011347 resin Substances 0.000 claims 5
- 229920002684 Sepharose Polymers 0.000 claims 4
- 150000001450 anions Chemical class 0.000 claims 3
- 150000001768 cations Chemical class 0.000 claims 3
- 239000012528 membrane Substances 0.000 claims 3
- 229920000936 Agarose Polymers 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 229920001429 chelating resin Polymers 0.000 claims 2
- 230000014509 gene expression Effects 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 239000013017 sartobind Substances 0.000 claims 2
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 claims 2
- PUAQLLVFLMYYJJ-UHFFFAOYSA-N 2-aminopropiophenone Chemical compound CC(N)C(=O)C1=CC=CC=C1 PUAQLLVFLMYYJJ-UHFFFAOYSA-N 0.000 claims 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims 1
- UEEJHVSXFDXPFK-UHFFFAOYSA-N N-dimethylaminoethanol Chemical compound CN(C)CCO UEEJHVSXFDXPFK-UHFFFAOYSA-N 0.000 claims 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000000654 additive Substances 0.000 claims 1
- 150000001412 amines Chemical class 0.000 claims 1
- YOUGRGFIHBUKRS-UHFFFAOYSA-N benzyl(trimethyl)azanium Chemical compound C[N+](C)(C)CC1=CC=CC=C1 YOUGRGFIHBUKRS-UHFFFAOYSA-N 0.000 claims 1
- 239000013622 capto Q Substances 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 239000001768 carboxy methyl cellulose Substances 0.000 claims 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims 1
- QYJXLKYOBNZROU-UHFFFAOYSA-N carboxysulfonylformic acid Chemical compound OC(=O)S(=O)(=O)C(O)=O QYJXLKYOBNZROU-UHFFFAOYSA-N 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000004440 column chromatography Methods 0.000 claims 1
- 239000000470 constituent Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 claims 1
- 239000002158 endotoxin Substances 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 229920000768 polyamine Polymers 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 125000001453 quaternary ammonium group Chemical group 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- AGGIJOLULBJGTQ-UHFFFAOYSA-N sulfoacetic acid Chemical compound OC(=O)CS(O)(=O)=O AGGIJOLULBJGTQ-UHFFFAOYSA-N 0.000 claims 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78431106P | 2006-03-20 | 2006-03-20 | |
| PCT/US2007/006015 WO2007108955A1 (en) | 2006-03-20 | 2007-03-09 | Protein purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009530378A JP2009530378A (ja) | 2009-08-27 |
| JP2009530378A5 true JP2009530378A5 (https=) | 2010-04-15 |
Family
ID=38226591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009501443A Withdrawn JP2009530378A (ja) | 2006-03-20 | 2007-03-09 | タンパク質精製法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20100190210A1 (https=) |
| EP (1) | EP2001898A1 (https=) |
| JP (1) | JP2009530378A (https=) |
| KR (1) | KR20080111487A (https=) |
| CN (1) | CN101437839A (https=) |
| AU (1) | AU2007227700A1 (https=) |
| CA (1) | CA2645739A1 (https=) |
| WO (1) | WO2007108955A1 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1869067A1 (en) * | 2005-04-11 | 2007-12-26 | Medarex, Inc. | Protein purification using hcic amd ion exchange chromatography |
| DE102007012439A1 (de) * | 2007-03-15 | 2008-09-18 | Emsland-Stärke GmbH | Verfahren zur Gewinnung pflanzlicher Proteine und/oder Peptide, danach hergestellte Proteine und/oder Peptide und Verwendung derselben |
| US9433922B2 (en) * | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
| ES2533266T5 (es) * | 2007-10-30 | 2018-04-18 | Genentech, Inc. | Purificación de anticuerpos mediante cromatografía de intercambio catiónico |
| BRPI0823046B1 (pt) | 2008-08-14 | 2021-12-14 | Genentech, Inc | Método para purificar um anticorpo monoclonal de uma composição compreendendo o anticorpo monoclonal e pelo menos uma proteína de ovário de hamster chinês (chop) |
| WO2010043703A1 (en) * | 2008-10-17 | 2010-04-22 | Dsm Ip Assets B.V. | Removal of host cell proteins |
| JP2012513425A (ja) | 2008-12-22 | 2012-06-14 | エフ．ホフマン−ラ ロシュ アーゲー | 免疫グロブリンの精製 |
| EP3309168A1 (en) * | 2009-08-06 | 2018-04-18 | F. Hoffmann-La Roche AG | Method to improve virus removal in protein purification |
| EP2480561B1 (en) | 2009-09-23 | 2016-07-13 | E. R. Squibb & Sons, L.L.C. | Cation exchange chromatography |
| ES2982297T3 (es) | 2009-12-18 | 2024-10-15 | Novartis Ag | Método de cromatografía de afinidad |
| WO2011073235A1 (en) | 2009-12-18 | 2011-06-23 | Csl Ltd. | Method of purifying polypeptides |
| GB201012603D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Protein purification |
| WO2012030512A1 (en) | 2010-09-03 | 2012-03-08 | Percivia Llc. | Flow-through protein purification process |
| AU2011325341B2 (en) * | 2010-11-01 | 2015-12-17 | Dpx Holdings B.V. | Single unit ion exchange chromatography antibody purification |
| WO2012104339A1 (en) * | 2011-02-01 | 2012-08-09 | Novo Nordisk A/S | Purification of insulin |
| JP2012214408A (ja) * | 2011-03-31 | 2012-11-08 | Asahi Kasei Medical Co Ltd | 澄明液中に分散した不純物凝集体を除去することによるタンパク質の精製方法 |
| US20130034893A1 (en) * | 2011-08-05 | 2013-02-07 | Zhiyu Li | Methods for Coupling of Molecules to Metal/Metal Oxide Surfaces |
| JP6116565B2 (ja) * | 2011-08-25 | 2017-04-19 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 陽イオンおよび陰イオン交換クロマトグラフィー法 |
| US9150841B2 (en) | 2012-06-29 | 2015-10-06 | Shire Human Genetic Therapies, Inc. | Cells for producing recombinant iduronate-2-sulfatase |
| KR101380740B1 (ko) | 2012-06-29 | 2014-04-11 | 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 | 이듀로네이트-2-설파타제의 정제 |
| CN105026418B (zh) * | 2013-02-06 | 2019-01-11 | 新加坡科技研究局 | 蛋白质纯化方法 |
| KR101569783B1 (ko) | 2013-06-05 | 2015-11-19 | 한화케미칼 주식회사 | 항체의 정제 방법 |
| JP6373376B2 (ja) * | 2013-11-15 | 2018-08-15 | ノバルティス アーゲー | 残留細胞培養不純物の除去 |
| US20170191101A1 (en) * | 2014-03-17 | 2017-07-06 | Regeneron Pharmaceuticals, Inc. | Clarification of mammalian cell culture |
| CN104693303B (zh) * | 2015-02-28 | 2018-03-13 | 苏州金盟生物技术有限公司 | 一种蛋白制品中的核酸去除方法 |
| ES3006358T3 (en) | 2015-03-13 | 2025-03-18 | Novimmune Sa | Methods of purifying bispecific antibodies |
| US10947268B2 (en) | 2016-08-12 | 2021-03-16 | Bristol-Myers Squibb Company | Methods of purifying proteins |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| KR102403334B1 (ko) * | 2019-02-14 | 2022-06-02 | 고려대학교 산학협력단 | 생물학적 샘플로부터 미세 소포체를 추출하는 방법 |
| EP3996828A4 (en) * | 2019-07-09 | 2023-09-06 | Tanvex Biopharma Usa, Inc. | TEMPERATURE-CONTROLLED PURIFICATION OF GRANULOCYTE COLONY STIMULATING FACTOR |
| IL292594A (en) * | 2019-11-07 | 2022-07-01 | Amgen Inc | Training with a high salt load in cation exchange chromatography to remove impurities associated with the product |
| KR102154952B1 (ko) * | 2020-02-21 | 2020-09-10 | 프레스티지바이오로직스 주식회사 | 아달리무맙의 Non-Protein A 정제방법 |
| KR102153258B1 (ko) * | 2020-02-21 | 2020-09-07 | 프레스티지바이오로직스 주식회사 | 베바시주맙 정제의 최적화된 방법 |
| CN113845560A (zh) * | 2020-06-28 | 2021-12-28 | 佛山汉腾生物科技有限公司 | 目的蛋白纯化方法 |
| CN118290514B (zh) * | 2024-06-05 | 2025-04-04 | 东曜药业有限公司 | 基于阴离子膜的抗体偶联药物的纯化方法及抗体偶联药物 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998039653A1 (en) * | 1997-03-06 | 1998-09-11 | University Of Washington | Methods for producing pure perlecan and other heparan sulfate proteoglycans |
| CA2473144C (en) * | 2002-02-05 | 2013-05-28 | Genentech, Inc. | Protein purification |
| WO2003102132A2 (en) * | 2002-04-26 | 2003-12-11 | Genetech, Inc. | Non-affinity purification of proteins |
| KR101150050B1 (ko) * | 2004-02-27 | 2012-07-05 | 지이 헬스케어 바이오-사이언시스 에이비 | 항체 정제 방법 |
| GB0425569D0 (en) * | 2004-11-19 | 2004-12-22 | Celltech R&D Ltd | Biological products |
| WO2007075283A2 (en) * | 2005-12-06 | 2007-07-05 | Amgen Inc. | Polishing steps used in multi-step protein purification processes |
| CN102887955A (zh) * | 2006-04-05 | 2013-01-23 | 艾博特生物技术有限公司 | 抗体纯化 |
-
2007
- 2007-03-09 CA CA002645739A patent/CA2645739A1/en not_active Abandoned
- 2007-03-09 EP EP07752697A patent/EP2001898A1/en not_active Withdrawn
- 2007-03-09 CN CNA2007800163473A patent/CN101437839A/zh active Pending
- 2007-03-09 US US12/293,732 patent/US20100190210A1/en not_active Abandoned
- 2007-03-09 AU AU2007227700A patent/AU2007227700A1/en not_active Abandoned
- 2007-03-09 JP JP2009501443A patent/JP2009530378A/ja not_active Withdrawn
- 2007-03-09 KR KR1020087025424A patent/KR20080111487A/ko not_active Withdrawn
- 2007-03-09 WO PCT/US2007/006015 patent/WO2007108955A1/en not_active Ceased
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