CA2645739A1 - Protein purification - Google Patents

Protein purification Download PDF

Info

Publication number
CA2645739A1
CA2645739A1 CA002645739A CA2645739A CA2645739A1 CA 2645739 A1 CA2645739 A1 CA 2645739A1 CA 002645739 A CA002645739 A CA 002645739A CA 2645739 A CA2645739 A CA 2645739A CA 2645739 A1 CA2645739 A1 CA 2645739A1
Authority
CA
Canada
Prior art keywords
protein
antibody
interest
exchange chromatography
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002645739A
Other languages
English (en)
French (fr)
Inventor
Alahari Arunakumari
Gisela Maria Marques Ferreira
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ER Squibb and Sons LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2645739A1 publication Critical patent/CA2645739A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA002645739A 2006-03-20 2007-03-09 Protein purification Abandoned CA2645739A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78431106P 2006-03-20 2006-03-20
US60/784,311 2006-03-20
PCT/US2007/006015 WO2007108955A1 (en) 2006-03-20 2007-03-09 Protein purification

Publications (1)

Publication Number Publication Date
CA2645739A1 true CA2645739A1 (en) 2007-09-27

Family

ID=38226591

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002645739A Abandoned CA2645739A1 (en) 2006-03-20 2007-03-09 Protein purification

Country Status (8)

Country Link
US (1) US20100190210A1 (https=)
EP (1) EP2001898A1 (https=)
JP (1) JP2009530378A (https=)
KR (1) KR20080111487A (https=)
CN (1) CN101437839A (https=)
AU (1) AU2007227700A1 (https=)
CA (1) CA2645739A1 (https=)
WO (1) WO2007108955A1 (https=)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1869067A1 (en) * 2005-04-11 2007-12-26 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
DE102007012439A1 (de) * 2007-03-15 2008-09-18 Emsland-Stärke GmbH Verfahren zur Gewinnung pflanzlicher Proteine und/oder Peptide, danach hergestellte Proteine und/oder Peptide und Verwendung derselben
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
ES2533266T5 (es) * 2007-10-30 2018-04-18 Genentech, Inc. Purificación de anticuerpos mediante cromatografía de intercambio catiónico
BRPI0823046B1 (pt) 2008-08-14 2021-12-14 Genentech, Inc Método para purificar um anticorpo monoclonal de uma composição compreendendo o anticorpo monoclonal e pelo menos uma proteína de ovário de hamster chinês (chop)
WO2010043703A1 (en) * 2008-10-17 2010-04-22 Dsm Ip Assets B.V. Removal of host cell proteins
JP2012513425A (ja) 2008-12-22 2012-06-14 エフ．ホフマン−ラ ロシュ アーゲー 免疫グロブリンの精製
EP3309168A1 (en) * 2009-08-06 2018-04-18 F. Hoffmann-La Roche AG Method to improve virus removal in protein purification
EP2480561B1 (en) 2009-09-23 2016-07-13 E. R. Squibb & Sons, L.L.C. Cation exchange chromatography
ES2982297T3 (es) 2009-12-18 2024-10-15 Novartis Ag Método de cromatografía de afinidad
WO2011073235A1 (en) 2009-12-18 2011-06-23 Csl Ltd. Method of purifying polypeptides
GB201012603D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
WO2012030512A1 (en) 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
AU2011325341B2 (en) * 2010-11-01 2015-12-17 Dpx Holdings B.V. Single unit ion exchange chromatography antibody purification
WO2012104339A1 (en) * 2011-02-01 2012-08-09 Novo Nordisk A/S Purification of insulin
JP2012214408A (ja) * 2011-03-31 2012-11-08 Asahi Kasei Medical Co Ltd 澄明液中に分散した不純物凝集体を除去することによるタンパク質の精製方法
US20130034893A1 (en) * 2011-08-05 2013-02-07 Zhiyu Li Methods for Coupling of Molecules to Metal/Metal Oxide Surfaces
JP6116565B2 (ja) * 2011-08-25 2017-04-19 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 陽イオンおよび陰イオン交換クロマトグラフィー法
US9150841B2 (en) 2012-06-29 2015-10-06 Shire Human Genetic Therapies, Inc. Cells for producing recombinant iduronate-2-sulfatase
KR101380740B1 (ko) 2012-06-29 2014-04-11 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 이듀로네이트-2-설파타제의 정제
CN105026418B (zh) * 2013-02-06 2019-01-11 新加坡科技研究局 蛋白质纯化方法
KR101569783B1 (ko) 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
JP6373376B2 (ja) * 2013-11-15 2018-08-15 ノバルティス アーゲー 残留細胞培養不純物の除去
US20170191101A1 (en) * 2014-03-17 2017-07-06 Regeneron Pharmaceuticals, Inc. Clarification of mammalian cell culture
CN104693303B (zh) * 2015-02-28 2018-03-13 苏州金盟生物技术有限公司 一种蛋白制品中的核酸去除方法
ES3006358T3 (en) 2015-03-13 2025-03-18 Novimmune Sa Methods of purifying bispecific antibodies
US10947268B2 (en) 2016-08-12 2021-03-16 Bristol-Myers Squibb Company Methods of purifying proteins
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102403334B1 (ko) * 2019-02-14 2022-06-02 고려대학교 산학협력단 생물학적 샘플로부터 미세 소포체를 추출하는 방법
EP3996828A4 (en) * 2019-07-09 2023-09-06 Tanvex Biopharma Usa, Inc. TEMPERATURE-CONTROLLED PURIFICATION OF GRANULOCYTE COLONY STIMULATING FACTOR
IL292594A (en) * 2019-11-07 2022-07-01 Amgen Inc Training with a high salt load in cation exchange chromatography to remove impurities associated with the product
KR102154952B1 (ko) * 2020-02-21 2020-09-10 프레스티지바이오로직스 주식회사 아달리무맙의 Non-Protein A 정제방법
KR102153258B1 (ko) * 2020-02-21 2020-09-07 프레스티지바이오로직스 주식회사 베바시주맙 정제의 최적화된 방법
CN113845560A (zh) * 2020-06-28 2021-12-28 佛山汉腾生物科技有限公司 目的蛋白纯化方法
CN118290514B (zh) * 2024-06-05 2025-04-04 东曜药业有限公司 基于阴离子膜的抗体偶联药物的纯化方法及抗体偶联药物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998039653A1 (en) * 1997-03-06 1998-09-11 University Of Washington Methods for producing pure perlecan and other heparan sulfate proteoglycans
CA2473144C (en) * 2002-02-05 2013-05-28 Genentech, Inc. Protein purification
WO2003102132A2 (en) * 2002-04-26 2003-12-11 Genetech, Inc. Non-affinity purification of proteins
KR101150050B1 (ko) * 2004-02-27 2012-07-05 지이 헬스케어 바이오-사이언시스 에이비 항체 정제 방법
GB0425569D0 (en) * 2004-11-19 2004-12-22 Celltech R&D Ltd Biological products
WO2007075283A2 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
CN102887955A (zh) * 2006-04-05 2013-01-23 艾博特生物技术有限公司 抗体纯化

Also Published As

Publication number Publication date
EP2001898A1 (en) 2008-12-17
AU2007227700A1 (en) 2007-09-27
JP2009530378A (ja) 2009-08-27
US20100190210A1 (en) 2010-07-29
WO2007108955A1 (en) 2007-09-27
CN101437839A (zh) 2009-05-20
WO2007108955A9 (en) 2008-11-06
KR20080111487A (ko) 2008-12-23

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Legal Events

Date Code Title Description
FZDE Discontinued