JP2009511056A5 - - Google Patents

Info

Publication number

JP2009511056A5

JP2009511056A5 JP2008535692A JP2008535692A JP2009511056A5 JP 2009511056 A5 JP2009511056 A5 JP 2009511056A5 JP 2008535692 A JP2008535692 A JP 2008535692A JP 2008535692 A JP2008535692 A JP 2008535692A JP 2009511056 A5 JP2009511056 A5 JP 2009511056A5

Authority

JP

Japan

Prior art keywords

seq

mrej type

detection

nos

primers

Prior art date

2006-10-10

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000001514 detection method Methods 0.000 claims 75
• 239000000523 sample Substances 0.000 claims 67
• 238000000034 method Methods 0.000 claims 47
• 108091034117 Oligonucleotide Proteins 0.000 claims 13
• 230000003321 amplification Effects 0.000 claims 5
• 238000003199 nucleic acid amplification method Methods 0.000 claims 5
• 108091093088 Amplicon Proteins 0.000 claims 4
• 150000007523 nucleic acids Chemical class 0.000 claims 4
• 108020004707 nucleic acids Proteins 0.000 claims 3
• 102000039446 nucleic acids Human genes 0.000 claims 3
• 239000002773 nucleotide Substances 0.000 claims 3
• 125000003729 nucleotide group Chemical group 0.000 claims 3
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
• 238000004458 analytical method Methods 0.000 claims 2
• 238000000137 annealing Methods 0.000 claims 2
• 239000013611 chromosomal DNA Substances 0.000 claims 2
• 238000005096 rolling process Methods 0.000 claims 2
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
• 108020004414 DNA Proteins 0.000 claims 1
• 238000001712 DNA sequencing Methods 0.000 claims 1
• RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims 1
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
• 108010066717 Q beta Replicase Proteins 0.000 claims 1
• 241000239226 Scorpiones Species 0.000 claims 1
• 241000191967 Staphylococcus aureus Species 0.000 claims 1
• 238000000246 agarose gel electrophoresis Methods 0.000 claims 1
• 238000004737 colorimetric analysis Methods 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• 238000006073 displacement reaction Methods 0.000 claims 1
• 238000000684 flow cytometry Methods 0.000 claims 1
• 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 1
• 238000004949 mass spectrometry Methods 0.000 claims 1
• 238000005259 measurement Methods 0.000 claims 1
• 101150008979 mecA gene Proteins 0.000 claims 1
• 229960003085 meticillin Drugs 0.000 claims 1
• 239000002105 nanoparticle Substances 0.000 claims 1
• 230000010287 polarization Effects 0.000 claims 1
• 238000004313 potentiometry Methods 0.000 claims 1
• 239000007790 solid phase Substances 0.000 claims 1
• GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims 1