JP2009050178A - 連続発酵による化学品の製造方法 - Google Patents
連続発酵による化学品の製造方法 Download PDFInfo
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- JP2009050178A JP2009050178A JP2007217976A JP2007217976A JP2009050178A JP 2009050178 A JP2009050178 A JP 2009050178A JP 2007217976 A JP2007217976 A JP 2007217976A JP 2007217976 A JP2007217976 A JP 2007217976A JP 2009050178 A JP2009050178 A JP 2009050178A
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- Prior art keywords
- membrane
- fermentation
- lactic acid
- porous
- continuous fermentation
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Abstract
【解決手段】本発明は、発酵培養液を多孔性分離膜によって濾液と未濾過液に分離し、濾液から所望の発酵生産物である化学品を回収するとともに、未濾過液を発酵培養液に保持または還流する連続発酵方法において、反応槽内の化学的消泡剤の濃度を1ppm以上150ppm以下にすることで、低い膜間差圧で安定に濾過処理でき、発酵による化学品の生産効率を著しく向上させることが可能となる。
【選択図】なし
Description
(1)微生物または培養細胞の培養液を、平均細孔径が0.01μm以上1μm未満の多孔性分離膜で濾過し、濾液から生産物を回収するとともに未濾過液を培養液に保持または還流し、かつ、発酵原料を培養液に追加する連続発酵による化学品の製造方法において、培養液中の化学的消泡剤の濃度が1ppm以上150ppm以下であることを特徴とする連続発酵による化学品の製造方法。
(2)物理的な消泡手段で消泡する(1)記載の連続発酵による化学品の製造方法。
(3)多孔性分離膜の膜素材にポリフッ化ビニリデンを用いる(1)または(2)に記載の連続発酵による化学品の製造方法。
(4)化学品が乳酸である(1)〜(3)のいずれかに記載の連続発酵による化学品の製造方法。
(5)微生物が酵母である(1)〜(4)のいずれかに記載の連続発酵による化学品の製造方法。
乳酸生産能力を持つ酵母株を下記のように造成した。具体的には、ヒト由来LDH遺伝子を酵母ゲノム上のPDC1プロモーターの下流に連結することでL−乳酸生産能力を持つ酵母株を造成する。ポリメラーゼ・チェーン・リアクション(PCR)には、La−Taq(宝酒造)、あるいはKOD-Plus-polymerase(東洋紡)を用い、付属の取扱説明に従って行った。
移動相:5mM p−トルエンスルホン酸(流速0.8mL/min)
反応液:5mM p−トルエンスルホン酸、20mM ビストリス、0.1mM EDTA・2Na(流速0.8mL/min)
検出方法:電気伝導度
温度:45℃。
移動相:1mM 硫酸銅水溶液
流速:1.0ml/min
検出方法:UV254nm
温度:30℃
また、L−乳酸の光学純度は次式で計算される。
ここで、LはL−乳酸の濃度、DはD−乳酸の濃度を表す。
実施例1 膜分離型連続発酵装置を用いた連続発酵によるL−乳酸の製造
膜分離型連続発酵装置を稼働させることにより、L−乳酸連続発酵系が得られるかどうかを調べるため、表1に示す組成の乳酸発酵培地を用い、この装置の連続発酵試験を行った。該培地は高圧蒸気滅菌(121℃、15分)して用いた。多孔性分離膜としてはポリフッ化ビニリデン(PVDF)を主成分とする多孔性分離膜を用いた。該多孔質分離膜の特性を調べたところ、平均細孔径が0.08μm、純水透過係数が50×10−9m3/m2/s/paであった。本実施例における運転条件は、特に断らない限り、以下のとおりである。
使用多孔性分離膜:PVDF製濾過膜
膜分離エレメント有効濾過面積:120平方cm
膜濾過流束:0.2m/日
温度調整:30(℃)
反応槽通気量:0.2(L/min)
反応槽攪拌速度:400(rpm)
pH調整:0.5N NaOHによりpH5に調整
発酵液循環装置による循環液量:0.1(L/min)
膜透過水量制御:膜分離槽水頭差により流量を制御(水頭差は2m以内で制御した。)。
実施例1の連続発酵の後、化学的消泡剤のブトキシポリプロピレングリコールの投入量を0.8g/日となるように6時間ごとに0.2g投入した。その結果、反応槽内の消泡剤の濃度が上昇し、それに伴い膜濾過差圧も急上昇し、膜濾過の安定運転が出来なくなった。運転結果を図3に示す。
実施例2 膜分離型連続発酵装置を用いた連続発酵によるL−乳酸の製造
実施例1と同条件で、L−乳酸の連続発酵を行った結果、460時間まで膜濾過の安定運転が可能であった。運転結果を図4に示す。
比較例2
実施例2の連続発酵の後、化学的消泡剤のブトキシポリプロピレングリコールを2g/日と大量投入した。その結果、膜濾過差圧は急上昇し、膜濾過の安定運転が出来なくなった。運転結果を図4に示す。
2 膜分離槽
3 膜分離エレメント
4 空気供給器
5 攪拌機
6 水頭差圧制御装置
7 培地供給ポンプ
8 pH調整溶液供給ポンプ
9 pHセンサ・制御装置
10 発酵液循環ポンプ
11 レベルセンサ
12 温度調節器
13 消泡剤投入ポンプ
14 物理的消泡手段
Claims (5)
- 微生物または培養細胞の培養液を、平均細孔径が0.01μm以上1μm未満の多孔性分離膜で濾過し、濾液から生産物を回収するとともに未濾過液を培養液に保持または還流し、かつ、発酵原料を培養液に追加する連続発酵による化学品の製造方法において、培養液中の化学的消泡剤の濃度が1ppm以上150ppm以下であることを特徴とする連続発酵による化学品の製造方法。
- 物理的な消泡手段で消泡する請求項1記載の連続発酵による化学品の製造方法。
- 多孔性分離膜の膜素材にポリフッ化ビニリデンを用いる請求項1または2に記載の連続発酵による化学品の製造方法。
- 化学品が乳酸である請求項1〜3のいずれかに記載の連続発酵による化学品の製造方法。
- 微生物が酵母である請求項1〜4のいずれかに記載の連続発酵による化学品の製造方法。
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WO2014156998A1 (ja) * | 2013-03-28 | 2014-10-02 | 旭硝子株式会社 | 化成品の製造方法および製造装置 |
WO2016140045A1 (ja) * | 2015-03-02 | 2016-09-09 | 三菱レイヨン株式会社 | 界面活性剤含有アミド化合物水溶液 |
CN111109591A (zh) * | 2019-10-25 | 2020-05-08 | 新疆红旗坡农业发展集团有限公司 | 肠道微生态高效重建型苹果酵素及加工技术 |
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WO2014156998A1 (ja) * | 2013-03-28 | 2014-10-02 | 旭硝子株式会社 | 化成品の製造方法および製造装置 |
WO2016140045A1 (ja) * | 2015-03-02 | 2016-09-09 | 三菱レイヨン株式会社 | 界面活性剤含有アミド化合物水溶液 |
JPWO2016140045A1 (ja) * | 2015-03-02 | 2017-04-27 | 三菱レイヨン株式会社 | 界面活性剤含有アミド化合物水溶液 |
CN111109591A (zh) * | 2019-10-25 | 2020-05-08 | 新疆红旗坡农业发展集团有限公司 | 肠道微生态高效重建型苹果酵素及加工技术 |
WO2023054556A1 (ja) * | 2021-09-30 | 2023-04-06 | 富士フイルム株式会社 | 細胞培養方法、及び有用物質の製造方法 |
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