JP2008173116A5 - - Google Patents

Info

Publication number

JP2008173116A5

JP2008173116A5 JP2007254762A JP2007254762A JP2008173116A5 JP 2008173116 A5 JP2008173116 A5 JP 2008173116A5 JP 2007254762 A JP2007254762 A JP 2007254762A JP 2007254762 A JP2007254762 A JP 2007254762A JP 2008173116 A5 JP2008173116 A5 JP 2008173116A5

Authority

JP

Japan

Prior art keywords

amino acid

isoleucine

acid sequence

seq

protein

Prior art date

2007-09-28

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 150000001413 amino acids Chemical group 0.000 claims 31
• 108090000623 proteins and genes Proteins 0.000 claims 26
• 102000004169 proteins and genes Human genes 0.000 claims 23
• 230000000694 effects Effects 0.000 claims 20
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 19
• 101000840545 Bacillus thuringiensis L-isoleucine-4-hydroxylase Proteins 0.000 claims 19
• OSCCDBFHNMXNME-UHFFFAOYSA-N gamma-hydroxyisoleucine Natural products CC(O)C(C)C(N)C(O)=O OSCCDBFHNMXNME-UHFFFAOYSA-N 0.000 claims 12
• 229960000310 isoleucine Drugs 0.000 claims 12
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 10
• 238000004519 manufacturing process Methods 0.000 claims 10
• 108020004414 DNA Proteins 0.000 claims 9
• 102000016680 Dioxygenases Human genes 0.000 claims 9
• 108010028143 Dioxygenases Proteins 0.000 claims 9
• 229930182844 L-isoleucine Natural products 0.000 claims 8
• 238000000034 method Methods 0.000 claims 7
• 241000894006 Bacteria Species 0.000 claims 6
• 238000007792 addition Methods 0.000 claims 6
• 125000000539 amino acid group Chemical group 0.000 claims 6
• 238000012217 deletion Methods 0.000 claims 6
• 230000037430 deletion Effects 0.000 claims 6
• 238000005805 hydroxylation reaction Methods 0.000 claims 6
• 238000003780 insertion Methods 0.000 claims 6
• 230000037431 insertion Effects 0.000 claims 6
• 244000005700 microbiome Species 0.000 claims 6
• 238000006467 substitution reaction Methods 0.000 claims 6
• 238000006243 chemical reaction Methods 0.000 claims 5
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 4
• 241000193830 Bacillus <bacterium> Species 0.000 claims 4
• 239000003125 aqueous solvent Substances 0.000 claims 4
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 4
• 150000003839 salts Chemical class 0.000 claims 4
• OSCCDBFHNMXNME-WDCZJNDASA-N (2s,3s,4r)-2-amino-4-hydroxy-3-methylpentanoic acid Chemical compound C[C@@H](O)[C@@H](C)[C@H](N)C(O)=O OSCCDBFHNMXNME-WDCZJNDASA-N 0.000 claims 3
• 102000008109 Mixed Function Oxygenases Human genes 0.000 claims 3
• 108010074633 Mixed Function Oxygenases Proteins 0.000 claims 3
• KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims 2
• 241000193388 Bacillus thuringiensis Species 0.000 claims 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 2
• 108020004511 Recombinant DNA Proteins 0.000 claims 2
• 229960005070 ascorbic acid Drugs 0.000 claims 2
• 235000010323 ascorbic acid Nutrition 0.000 claims 2
• 239000011668 ascorbic acid Substances 0.000 claims 2
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 2
• 229940097012 bacillus thuringiensis Drugs 0.000 claims 2
• 229910052760 oxygen Inorganic materials 0.000 claims 2
• 239000001301 oxygen Substances 0.000 claims 2
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 2
• OSCCDBFHNMXNME-DSDZBIDZSA-N 4-Hydroxy-L-isoleucine Chemical compound CC(O)[C@H](C)[C@H](N)C(O)=O OSCCDBFHNMXNME-DSDZBIDZSA-N 0.000 claims 1
• 241000186063 Arthrobacter Species 0.000 claims 1
• 241000228212 Aspergillus Species 0.000 claims 1
• 241001037822 Bacillus bacterium Species 0.000 claims 1
• 241000194108 Bacillus licheniformis Species 0.000 claims 1
• 244000063299 Bacillus subtilis Species 0.000 claims 1
• 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
• 241000186216 Corynebacterium Species 0.000 claims 1
• 241000186226 Corynebacterium glutamicum Species 0.000 claims 1
• 241000588722 Escherichia Species 0.000 claims 1
• 241000588724 Escherichia coli Species 0.000 claims 1
• 241000193386 Lysinibacillus sphaericus Species 0.000 claims 1
• 241000203720 Pimelobacter simplex Species 0.000 claims 1
• 241000589516 Pseudomonas Species 0.000 claims 1
• 230000003698 anagen phase Effects 0.000 claims 1
• 230000001580 bacterial effect Effects 0.000 claims 1
• 150000001768 cations Chemical class 0.000 claims 1
• 239000013592 cell lysate Substances 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 230000033444 hydroxylation Effects 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 239000002773 nucleotide Substances 0.000 claims 1
• 125000003729 nucleotide group Chemical group 0.000 claims 1
• 230000001105 regulatory effect Effects 0.000 claims 1
• 241000894007 species Species 0.000 claims 1