JP2008173116A5 - - Google Patents

Download PDF

Info

Publication number
JP2008173116A5
JP2008173116A5 JP2007254762A JP2007254762A JP2008173116A5 JP 2008173116 A5 JP2008173116 A5 JP 2008173116A5 JP 2007254762 A JP2007254762 A JP 2007254762A JP 2007254762 A JP2007254762 A JP 2007254762A JP 2008173116 A5 JP2008173116 A5 JP 2008173116A5
Authority
JP
Japan
Prior art keywords
amino acid
isoleucine
acid sequence
seq
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2007254762A
Other languages
Japanese (ja)
Other versions
JP5246639B2 (en
JP2008173116A (en
Filing date
Publication date
Application filed filed Critical
Priority to JP2007254762A priority Critical patent/JP5246639B2/en
Priority claimed from JP2007254762A external-priority patent/JP5246639B2/en
Publication of JP2008173116A publication Critical patent/JP2008173116A/en
Publication of JP2008173116A5 publication Critical patent/JP2008173116A5/ja
Application granted granted Critical
Publication of JP5246639B2 publication Critical patent/JP5246639B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Claims (25)

微生物由来の、イソロイシンから４−ヒドロキシイソロイシンを生成する水酸化酵素存在下でイソロイシン又はその塩を水酸化反応に供し、４−ヒドロキシイソロイシンを生成させる工程を含むことを特徴とする、４−ヒドロキシイソロイシン又はその塩の製造方法。 4-hydroxyisoleucine characterized in that it comprises a step of producing 4-hydroxyisoleucine by subjecting isoleucine or a salt thereof to a hydroxylation reaction in the presence of a hydroxylase that produces 4-hydroxyisoleucine from isoleucine derived from a microorganism. Or the manufacturing method of the salt. 水酸化反応が、Hydroxylation reaction
(f) 配列番号２、８、１３、１７又は２１のアミノ酸配列を有するタンパク質、(f) a protein having the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21;
(g)配列番号２、８、１３、１７又は２１のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質、及び、(g) L-isoleucine having an amino acid sequence comprising substitution, deletion, insertion, addition or inversion of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 A protein having dioxygenase activity, and
(h)配列番号２、８、１３、１７又は２１のアミノ酸配列に対し少なくとも７０％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質からなる群から選ばれる少なくとも１種のL-イソロイシンジオキシゲナーゼの存在下で水性溶媒中で行われる、請求項１記載の製造方法。(h) at least one selected from the group consisting of proteins having an amino acid sequence showing at least 70% homology to the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 and having L-isoleucine dioxygenase activity The production method according to claim 1, which is carried out in an aqueous solvent in the presence of a seed L-isoleucine dioxygenase. 水酸化反応が、Hydroxylation reaction
(f) 配列番号２、８、１３、１７又は２１のアミノ酸配列を有するタンパク質、(f) a protein having the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21;
(g)配列番号２、８、１３、１７又は２１のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質、及び、(g) L-isoleucine having an amino acid sequence comprising substitution, deletion, insertion, addition or inversion of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 A protein having dioxygenase activity, and
(h)配列番号２、８、１３、１７又は２１のアミノ酸配列に対し少なくとも７０％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質からなる群から選ばれるL-イソロイシンジオキシゲナーゼを含む細菌の存在下で水性溶媒中で行なわれる、請求項１の製造方法。(h) L- selected from the group consisting of proteins having an amino acid sequence showing at least 70% homology to the amino acid sequence of SEQ ID NO: 2, 8, 13, 17, or 21 and having L-isoleucine dioxygenase activity The production method according to claim 1, which is carried out in an aqueous solvent in the presence of a bacterium containing isoleucine dioxygenase. 微生物がバチルス属に属する微生物である、請求項１記載の製造方法。 The production method according to claim 1, wherein the microorganism is a microorganism belonging to the genus Bacillus. バチルス属に属する微生物がBacillus thuringiensis、Bacillus licheniformis、及び、Bacillus sphaericusから選択される微生物である、請求項４記載の製造方法。 Microorganism Bacillus thuringiensis belongs to the genus Bacillus, Bacillus licheniformis, and a microorganism selected from Bacillus sphaericus, a manufacturing method of claim 4 wherein. 水酸化反応が、微生物の対数増殖期細胞から調製された破砕液の存在下で行われる請求項１〜２及び４〜５のいずれか１項に記載の製造方法。 The production method according to any one of claims 1 to 2 and 4 to 5 , wherein the hydroxylation reaction is carried out in the presence of a disrupted solution prepared from logarithmic growth phase cells of a microorganism. L-イソロイシンを水酸化反応に供する、請求項１〜６のいずれか１項に記載の製造方法。 The production method according to any one of claims 1 to 6 , wherein L-isoleucine is subjected to a hydroxylation reaction. 水酸化酵素が、ジオキシゲナーゼである、請求項１〜７のいずれか１項に記載の製造方法。 The production method according to any one of claims 1 to 7 , wherein the hydroxylase is dioxygenase. 水酸化酵素が以下の性質を有する、請求項１〜８のいずれか１項に記載の製造方法。
（ａ）補因子として、酸素、Ｆｅ2+、アスコルビン酸、及び、２−オキソグルタル酸を要求する。
（ｂ）反応至適ｐＨがｐＨ５〜ｐＨ８。
（ｃ）反応至適温度が４５℃以下。
（ｄ）５０℃以上で失活する。
（ｅ）ＥＤＴＡやＣｕ2+、Ｚｎ2+により阻害される。 The production method according to any one of claims 1 to 8 , wherein the hydroxylase has the following properties.
(A) Oxygen, Fe 2+ , ascorbic acid, and 2-oxoglutaric acid are required as cofactors.
(B) The optimum pH for the reaction is pH 5 to pH 8.
(C) The optimum reaction temperature is 45 ° C. or lower.
(D) Deactivates at 50 ° C. or higher.
(E) It is inhibited by EDTA, Cu 2+ and Zn 2+ . 以下の性質を有し、バチルス属細菌から単離されうるジオキシゲナーゼ。
（ａ）補因子として、酸素、Ｆｅ2+、アスコルビン酸、及び、２−オキソグルタル酸を要求する。
（ｂ）反応至適ｐＨがｐＨ５〜ｐＨ８。
（ｃ）反応至適温度が４５℃以下。
（ｄ）５０℃以上で失活する。
（ｅ）ＥＤＴＡやＣｕ2+、Ｚｎ2+により阻害される。
（ｆ）ドデシル硫酸ナトリウム−ポリアクリルアミドゲル電気泳動法により測定される分子量が３１，０００±２０，０００のサブユニットからなる。
（ｇ）配列番号２に示すアミノ酸配列をＮ末端に有する。 A dioxygenase having the following properties and capable of being isolated from Bacillus bacteria.
(A) Oxygen, Fe 2+ , ascorbic acid, and 2-oxoglutaric acid are required as cofactors.
(B) The optimum pH for the reaction is pH 5 to pH 8.
(C) The optimum reaction temperature is 45 ° C. or lower.
(D) Deactivates at 50 ° C. or higher.
(E) It is inhibited by EDTA, Cu 2+ and Zn 2+ .
(F) It consists of subunits having a molecular weight of 31,000 ± 20,000 as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
(G) It has the amino acid sequence shown in SEQ ID NO: 2 at the N-terminus. バチルス属細菌が、Bacillus thuringiensisである、請求項１０記載のジオキシゲナーゼ。 The dioxygenase according to claim 10 , wherein the Bacillus bacterium is Bacillus thuringiensis. (a) 配列番号１の塩基配列を含むDNA、
(b) 配列番号１の塩基配列に相補的な塩基配列を有するDNAとストリンジェントな条件でハイブリダイズし、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質をコードするDNA、
(c) 配列番号２のアミノ酸配列を有するタンパク質をコードするDNA、
(d)配列番号２のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質をコードするDNA、及び、
(e)配列番号２のアミノ酸配列に対し少なくとも９８％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質をコードするDNA
からなる群から選ばれるDNA。 (a) DNA comprising the nucleotide sequence of SEQ ID NO: 1,
(b) a DNA that hybridizes with a DNA having a base sequence complementary to the base sequence of SEQ ID NO: 1 under stringent conditions and encodes a protein having L-isoleucine dioxygenase activity;
(c) DNA encoding a protein having the amino acid sequence of SEQ ID NO: 2,
(d) a protein having an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acid residues and having L-isoleucine dioxygenase activity in the amino acid sequence of SEQ ID NO: 2 DNA to do and
(e) DNA encoding a protein having an amino acid sequence showing at least 98% homology to the amino acid sequence of SEQ ID NO: 2 and having L-isoleucine dioxygenase activity
DNA selected from the group consisting of 請求項１２に記載のDNAとベクターDNAとを連結することにより得られる組換えDNA。 A recombinant DNA obtained by linking the DNA according to claim 12 and a vector DNA. 請求項１３に記載の組換えDNAにより形質転換された細胞。 A cell transformed with the recombinant DNA according to claim 13 . 請求項１４に記載の細胞を培地に培養し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質を、培地、細胞又はそれらの両方に蓄積させることを含む、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質の製造方法。 A method for producing a protein having L-isoleucine dioxygenase activity, comprising culturing the cell according to claim 14 in a medium, and accumulating the protein having L-isoleucine dioxygenase activity in the medium, cells or both. . (f) 配列番号２のアミノ酸配列を有するタンパク質、
(g)配列番号２のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質、及び、
(h)配列番号２のアミノ酸配列に対し少なくとも９８％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質
からなる群から選ばれるタンパク質。 (f) a protein having the amino acid sequence of SEQ ID NO: 2,
(g) a protein having an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2 and having L-isoleucine dioxygenase activity; and ,
(h) a protein selected from the group consisting of proteins having an amino acid sequence showing at least 98% homology to the amino acid sequence of SEQ ID NO: 2 and having L-isoleucine dioxygenase activity; 下記の性質を有するタンパク質。
(A)L-イソロイシンの水酸化により(2S,3R,4S)-4-ヒドロキシ-L-イソロイシンを生成する反応を触媒する活性を有する。
(B)前記活性が二価カチオンFe2+に依存する。
(C)SDS-PAGEにより測定した分子量が約29±2.0 kDaである。 A protein having the following properties:
(A) It has an activity of catalyzing a reaction for producing (2S, 3R, 4S) -4-hydroxy-L-isoleucine by hydroxylation of L-isoleucine.
(B) The activity depends on the divalent cation Fe 2+ .
(C) The molecular weight measured by SDS-PAGE is about 29 ± 2.0 kDa. (f) 配列番号２、８、１３、１７又は２１のアミノ酸配列を有するタンパク質、
(g)配列番号２、８、１３、１７又は２１のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質、及び、
(h)配列番号２、８、１３、１７又は２１のアミノ酸配列に対し少なくとも７０％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質からなる群から選ばれる少なくとも１種のL-イソロイシンジオキシゲナーゼの存在下で水性溶媒中でL-イソロイシンを反応させ、生成した (2S,3R,4S)-4-ヒドロキシ-L-イソロイシンを単離することを含む(2S,3R,4S)-4-ヒドロキシ-L-イソロイシン又はその塩の製造方法。 (f) a protein having the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21;
(g) L-isoleucine having an amino acid sequence comprising substitution, deletion, insertion, addition or inversion of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 A protein having dioxygenase activity, and
(h) at least one selected from the group consisting of proteins having an amino acid sequence showing at least 70% homology to the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 and having L-isoleucine dioxygenase activity Reacting L-isoleucine in an aqueous solvent in the presence of the species L-isoleucine dioxygenase and isolating the resulting (2S, 3R, 4S) -4-hydroxy-L-isoleucine (2S, 3R , 4S) -4-hydroxy-L-isoleucine or a salt thereof. (f) 配列番号２、８、１３、１７又は２１のアミノ酸配列を有するタンパク質、
(g)配列番号２、８、１３、１７又は２１のアミノ酸配列において、１又は数個のアミノ酸残基の置換、欠失、挿入、付加又は逆位を含むアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質、及び、
(h)配列番号２、８、１３、１７又は２１のアミノ酸配列に対し少なくとも７０％の相同性を示すアミノ酸配列を有し、L-イソロイシンジオキシゲナーゼ活性を有するタンパク質からなる群から選ばれるL-イソロイシンジオキシゲナーゼを含む細菌の存在下で水性溶媒中でL-イソロイシンを反応させ、生成した (2S,3R,4S)-4-ヒドロキシ-L-イソロイシンを単離することを含む(2S,3R,4S)-4-ヒドロキシ-L-イソロイシン又はその塩の製造方法。 (f) a protein having the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21;
(g) L-isoleucine having an amino acid sequence comprising substitution, deletion, insertion, addition or inversion of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2, 8, 13, 17 or 21 A protein having dioxygenase activity, and
(h) L- selected from the group consisting of proteins having an amino acid sequence showing at least 70% homology to the amino acid sequence of SEQ ID NO: 2, 8, 13, 17, or 21 and having L-isoleucine dioxygenase activity Reacting L-isoleucine in an aqueous solvent in the presence of bacteria containing isoleucine dioxygenase and isolating the resulting (2S, 3R, 4S) -4-hydroxy-L-isoleucine (2S, 3R, 4S) A process for producing 4-hydroxy-L-isoleucine or a salt thereof. 前記細菌がL-イソロイシンジオキシゲナーゼの活性が増強されるように改変されている請求項１９に記載の方法。 20. The method according to claim 19 , wherein the bacterium has been modified so that the activity of L-isoleucine dioxygenase is enhanced. L-イソロイシンジオキシゲナーゼの活性が、前記L-イソロイシンジオキシゲナーゼをコードする遺伝子の発現の増大により増強されている請求項２０に記載の方法。 21. The method according to claim 20 , wherein the activity of L-isoleucine dioxygenase is enhanced by an increase in expression of a gene encoding the L-isoleucine dioxygenase. L-イソロイシンジオキシゲナーゼの活性が、前記L-イソロイシンジオキシゲナーゼをコードする遺伝子の発現調節配列の改変、又は、前記L-イソロイシンジオキシゲナーゼをコードする遺伝子のコピー数の増大により増強されている請求項２１に記載の方法。 The activity of L-isoleucine dioxygenase is enhanced by modification of an expression regulatory sequence of a gene encoding the L-isoleucine dioxygenase or an increase in the copy number of the gene encoding the L-isoleucine dioxygenase. The method according to 21 . 前記細菌が、Escherichia, Pseudomonas, Corynebacterium, Arthrobacter, Aspergillus又は Bacillus属に属する請求項２０〜２２のいずれか一項に記載の方法。 The method according to any one of claims 20 to 22 , wherein the bacterium belongs to the genus Escherichia, Pseudomonas, Corynebacterium, Arthrobacter, Aspergillus, or Bacillus. 前記細菌が、Escherichia coli, Arthrobacter simplex, Corynebacterium glutamicum,
Arthrobactor globiformis, Arthrobactor sulfureus, Arthrobactor viscosus 又はBacillus subtilisに属する請求項２３に記載の方法。 The bacterium is Escherichia coli, Arthrobacter simplex, Corynebacterium glutamicum,
The method according to claim 23 , which belongs to Arthrobactor globiformis, Arthrobactor sulfureus, Arthrobactor viscosus or Bacillus subtilis. 前記細菌が、細菌培養物、細胞、細胞処理物又は細胞破砕液である請求項２０〜２４のいずれか１項に記載の方法。 The method according to any one of claims 20 to 24 , wherein the bacterium is a bacterial culture, a cell, a cell treatment product, or a cell lysate.
JP2007254762A 2006-09-28 2007-09-28 Process for producing 4-hydroxy-L-isoleucine Active JP5246639B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007254762A JP5246639B2 (en) 2006-09-28 2007-09-28 Process for producing 4-hydroxy-L-isoleucine

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP2006265452 2006-09-28
JP2006265452 2006-09-28
JP2006345461 2006-12-22
JP2006345461 2006-12-22
RU2007104645 2007-02-07
RU2007104645 2007-02-07
JP2007254762A JP5246639B2 (en) 2006-09-28 2007-09-28 Process for producing 4-hydroxy-L-isoleucine

Publications (3)

Publication Number Publication Date
JP2008173116A JP2008173116A (en) 2008-07-31
JP2008173116A5 true JP2008173116A5 (en) 2010-07-22
JP5246639B2 JP5246639B2 (en) 2013-07-24

Family

ID=39700639

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007254762A Active JP5246639B2 (en) 2006-09-28 2007-09-28 Process for producing 4-hydroxy-L-isoleucine

Country Status (2)

Country Link
JP (1) JP5246639B2 (en)
CN (1) CN101528940B (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2007147436A (en) * 2007-12-21 2009-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) BACTERIA-PRODUCER (2S, 3R, 4S) -4-HYDROXY-L-ISOLEUCIN AND METHOD FOR PRODUCTION (2S, 3R, 4S) -4-HYDROXY-L-ISOLEUCIN
JP2010098975A (en) * 2008-10-22 2010-05-06 Waseda Univ Method for producing l- and d-aliphatic amino acid hydroxide
JP5140879B1 (en) * 2012-06-22 2013-02-13 強化土株式会社 Ground improvement method
CN103865940B (en) * 2014-04-03 2016-05-11 天津科技大学 A kind of ILE '-hydroxylase gene and genetic engineering bacterium and application
CN104152505B (en) * 2014-08-08 2016-11-23 江南大学 A kind of method utilizing recombinant bacterial strain conversion to prepare 4HIL
CN105566136A (en) * 2016-01-19 2016-05-11 天津科技大学 Method for separating and extracting 4-hydroxyisoleucine from fermentation liquor
CN105969785B (en) * 2016-05-12 2019-11-22 天津科技大学 A kind of synthetic method of 4-hydroxyisoleucine
CN105779522B (en) * 2016-05-17 2016-12-28 河南巨龙生物工程股份有限公司 A kind of microbial enzyme conversion method produces the method for L 4 hydroxyisoleucine
EP3604505A4 (en) * 2017-03-31 2020-02-19 Kaneka Corporation Novel monooxygenase and utilization thereof
CN107475267B (en) * 2017-09-29 2020-07-14 天津科技大学 4-hydroxyisoleucine production plasmid and strain and synthesis method of 4-hydroxyisoleucine
CN108504639B (en) * 2018-04-03 2020-12-29 江南大学 Isoleucine dioxygenase mutant and application thereof
CN109628476B (en) * 2019-02-18 2021-01-29 江南大学 Method for producing 4-hydroxyisoleucine by using whole cell transformation
CN109929790B (en) * 2019-03-29 2020-12-01 江南大学 Genetic engineering bacterium for producing 4-hydroxyisoleucine and application thereof
CN110438097B (en) * 2019-08-14 2020-12-29 江南大学 L-isoleucine hydroxylase mutant with improved heat stability and enzyme activity
CN112280756B (en) * 2020-10-29 2024-04-16 华东理工大学 Isoleucine hydroxylase mutant and application thereof in synthesis of (2S, 3R, 4S) -4-hydroxyisoleucine
CN115141110B (en) * 2021-03-29 2023-12-12 安徽华恒生物科技股份有限公司 Continuous decoloring method of L-valine fermentation liquid
CN113233991A (en) * 2021-05-27 2021-08-10 无锡晶海氨基酸股份有限公司 Method for extracting 4-hydroxyisoleucine from whole-cell catalytic liquid

Similar Documents

Publication Publication Date Title
JP2008173116A5 (en)
RU2338784C2 (en) New aldolase, dna coding aldlase, cells transformed by dna, method of aldolase obtaining and method of obtaining of 4-hydroxy-l-isoleucine (versions)
Kim et al. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli
CN113621600B (en) High-activity nitrile hydratase mutant and application thereof
JP2020174686A (en) Production methods of 4-aminocinnamic acid using enzyme
WO2012002450A1 (en) D-succinylase, and method for producing d-amino acid using same
EP3147351B1 (en) Microorganism having improved intracellular energy level and method for producing l-amino acid using same
JPWO2019194062A1 (en) An enzyme capable of producing a galactooligosaccharide derived from Paenibacillus pavuli, and a method for producing a galactooligosaccharide.
JPWO2010067578A1 (en) Novel amino acid dehydrogenase and method for producing L-amino acid, 2-oxoacid, or D-amino acid
TW201035319A (en) Novel 2-deoxy-scyllo-inosose synthetase
JP4108095B2 (en) Novel nitrile hydratase
Yu et al. Identification, cloning, and expression of L-amino acid oxidase from marine Pseudoalteromonas sp. B3
WO2002072841A1 (en) Dna encoding hydantoinase, dna encoding n-carbamyl-l-amino acid hydrolase, recombinant dna, transformed cells, process for producing protein and process for producing optically active amino acid
CN107446903B (en) Salt-tolerant ethanol-tolerant pectinase with 3 optimal pH values and gene thereof
Wang et al. Cloning, expression and characterization of d-aminoacylase from Achromobacter xylosoxidans subsp. denitrificans ATCC 15173
JP5284947B2 (en) Novel amidase, gene thereof, vector, transformant, optically active carboxylic acid amide and method for producing optically active carboxylic acid using them
Hasegawa et al. Cloning and expression of the N, N-dimethylformamidase gene from Alcaligenes sp. strain KUFA-1
RU2402608C2 (en) Method of producing 4-hydroxy-l-isoleucine
EP1770166B1 (en) L-amino acid amide asymmetric hydrolase and dna encoding the same
JP4380874B2 (en) Alkaline cellulase gene
EP1496113A1 (en) Gluconate dehydratase
JP2019030314A (en) Recombinant microorganism for producing quinolinic acid and producing method for quinolinic acid using the same
EP4353820A1 (en) Superoxide dismutase 1 variant and method for producing glutathione or derivative thereof, using same
CN115948358B (en) L-aspartic oxidase SaAO, and coding gene and application thereof
SHAREEF et al. Heterologous Expression and Molecular Cloning from Williamsia Marianensis