A kind of microbial enzyme conversion method produces the method for L-4-hydroxyisoleucine
The present invention relates to technical field of microbial fermentation, be specifically related to a kind of microbial enzyme conversion method production L-4-hydroxyl different
Leucic method.
Background technology
L-4-hydroxyisoleucine (4-hydroxyisoleucine) is the nonprotein amino that a kind of nature exists
Acid.Research finds, L-4-hydroxyisoleucine has promotion insulin secretion, improves insulin resistant and regulation blood fat etc. in many ways
Face acts on.L-4-hydroxyisoleucine can not only promote that the picked-up of glucose is utilized by surrounding tissue, additionally it is possible to reduces liver
Glucose output is to improve the insulin resistant of peripheral tissues.Compare the medicine having been used to now treat T2DM, L-4-hydroxyl
The insulin synthesis of isoleucine regulation and control is strictly to rely on concentration of glucose.Therefore, L-4-hydroxyisoleucine will not cause class
Like side effect such as hypoglycemia.The most also find that L-4-hydroxyisoleucine can effectively reduce triglyceride and cholesterol in blood
Amount, has acceleration lipid metabolism, blood fat reducing and the effect of liver function protecting.Accordingly, as effectively prevention and treatment diabetes and
The ideal medicament of obesity, L-4-hydroxyisoleucine is with a wide range of applications and the market demand.
Being limited by technology and cost, the yield of China's L-4-hydroxyisoleucine can not meet domestic and foreign export
Demand.The L-4-hydroxyisoleucine production method of report includes extraction method, chemical synthesis and enzyme process at present, but only extracts
Method for industrialized production and there is extraction ratio difficulty low, isolated and purified, Raw Material Demand amount is big and high in cost of production is not enough, tradition
Chemical method whether organic synthesis or chemical resolution all lose competitiveness because production cost is high.Because of enzyme catalysis in biological method
Method preparation method efficiency is of a relatively high, but still suffers from complex operation, expensive starting materials, the problem that production cost is too high, and current
Lack the relevant Technology that can be used for industrialization large-scale production.
Summary of the invention
It is an object of the invention to the deficiency for solving above-mentioned technical problem, it is provided that a kind of microbial enzyme conversion method produces L-4-
The method of hydroxyisoleucine, uses high density fermentation and enzyme to live high, and reaction system is simply efficiently and extraction simple flow is easy,
Yield is up to 75% and product purity is more than 98%, and the method efficiency is high, low cost, it is easy to industrialization produces.
The present invention solves above-mentioned technical problem, the technical scheme provided is: a kind of microbial enzyme conversion method produces L-
The method of 4-hydroxyisoleucine, comprises the following steps:
(1), the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine will can be induced to access equipped with seed tank culture base
Seed tank in, at temperature 33 DEG C-37 DEG C, pH6.5-7.5, dissolved oxygen is cultivated to bacterium solution under conditions of controlling more than 25%
OD600 value is 12-16: obtain the Escherichia coli recombinant strain of the induced production L-4-hydroxyisoleucine of maturation;
The construction method of the described Escherichia coli recombinant strain inducing production L-4-hydroxyisoleucine is: according to Su Yunjin
In bacillus cereus YL-03, gene I/D O(i.e. encodes the gene of ILE hydroxylase) it is carried out manually modified, it is thus achieved that gene
Sequence is as shown in SEQ ID NO.1;Clone this gene, and by this gene constructed enter plasmid pET28a, obtain recombiant plasmid, will
Import e. coli bl21 after recombiant plasmid, i.e. obtain inducing the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine.
(2), the Escherichia coli recombinant strain of ripe induced production L-4-hydroxyisoleucine is accessed equipped with fermentation tank
In the fermentation tank of culture medium, carry out deep liquid ventilating fermentation cultivation;In incubation, when cultivating 5h in fermentation medium one
Secondary property puts into the IPTG(isopropyl-β-D-thiogalactoside of final concentration of 0.1-0.3mmol/L) to induce, dissolved oxygen controls
At 30-50%, pH6.5-7.0, after end sugar exhausts, stream adds glycerine water solution that mass concentration is 10-80% as carbon source, ferments
The control of Cheng Wendu two rank, before adding IPTG induction, control temperature is at 34-36 DEG C, after pending abduction delivering, adjustment temperature to 30-
Cultivate for 32 DEG C;
(3), after fermentation ends, by butterfly centrifugal machine cryogenic collector thalline, and use phosphate buffer that thalline is entered
Row washing, removes phage surface impurity, then with pure water preparation catalyst system and catalyzing: a-ketoglutaric acid 30-35g/L, isoleucine
26-30g/L, ferrous sulfate 0.5-4g/L, magnesium sulfate 0.1-1g/L, ascorbic acid 0.01-0.2g/L, add in catalyst system and catalyzing
Entering the thalline after washing, forced ventilation is catalyzed, and maintenance pH is 6.0-6.5, and catalytic temperature is 28-32 DEG C, obtains catalytic liquid;Described
The addition of thalline is: every 100ml catalyst system and catalyzing adds 0.3g thalline;
(4), that catalytic liquid first passes through ceramic membrane filter is degerming, after the ultrafilter membrane of 2-4Kd molecular weight, and takes off through activated carbon
Color, after using 122 weak-acid cation-exchange resin desalinations, 732 cationic exchange resin adsorption washing with ammonia on effluent
De-, evaporation and concentration obtains L-4-hydroxyisoleucine crude product, is then recrystallized to give L-4-hydroxyisoleucine.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
The formula stating seed tank culture base is: glucose 0.5-20g/L, yeast powder 0.5-10g/L, peptone 0.5-5g/L, ammonium sulfate
0.5-10g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.5-3g/L, vitamin B 10-20mg/L, biotin 0.5-
10mg/L, ferrous sulfate 0.5-50mg/L, manganese sulfate 0.5-50mg/L, defoamer 0-0.5ml/L, remaining is water.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
The formula stating fermentation tank culture medium is: glucose 0.5-10g/L, yeast powder 0.5-10g/L, peptone 0.5-5g/L, ammonium sulfate
0.5-10g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.5-3g/L, VB1 0.5-20mg/L, VH 0.5-10mg/L, sulphuric acid
Ferrous 0.5-50mg/L, manganese sulfate 0.5-50mg/L, Semen Maydis pulp 0.5-10mL, sodium citrate 0.5-2g/L. defoamer 0-
0.5ml/L, remaining is water.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
State catalytic liquid in step (4) and be first heated to 60-80 DEG C before ceramic membrane filter is degerming, and keep 10-40min.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
Adding the 0.1-5% that charcoal amount is material liquid volume when stating activated carbon decolorizing in step (4), temperature retention time is 30-60min.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
Stating the ammonia mass concentration of eluting in step (4) is 1%-3%, and elution flow rate is 1-1.5BV/h.
The further improvements in methods of L-4-hydroxyisoleucine are produced: step as the present invention a kind of microbial enzyme conversion method
Suddenly evaporation and concentration described in (4), the final concentration of 500-600g/L of 4-hydroxyisoleucine crude product after evaporation and concentration.
Beneficial effect
One, applicant is for the deficiency in current fermentation process, at University Of Science and Technology Of Tianjin, China's aminoacid technology clothes
Under the help at business center is instructed, by fermentating formula and process control, microbial enzyme conversion scheme and control technique, extraction side
Case and control the improvement of technique so that extraction process is simple, conversion ratio is up to more than 95.4%, and product purity up to 98% with
On, greatly improve production capacity and the efficiency of L-4-hydroxyisoleucine, the extensive of L-4-hydroxyisoleucine can be met
Industrialized production, has higher Social and economic benef@;
Two, the progress on the method and technology in the present invention includes following aspect: 1, fermentation culture: during the fermentation to sending out
Ferment process temperature carries out two rank controls, and earlier fermentation i.e. adds and controls temperature before IPTG pre-induction at 34-36 DEG C, pending induction
After expression, adjust temperature to 30-32 DEG C cultivation and carry out cooling expression;Reduce temperature after induction and can reduce thalline than growth speed
Rate, reduces plasmid loss rate, is of value to the formation of enzymatic structure, protects after preventing the expression of formation inclusion body, beneficially genes of interest
The biological activity of card enzyme molecule.Culture medium increases Semen Maydis pulp as organic nitrogen source, for thalli growth and expression of enzymes provide aminoacid,
Somatomedin etc..It is simultaneously introduced sodium citrate suppression EMP Embden Meyerbof Parnas pathway, reduces carbon metabolism flow loss, reduce the metabolic by-products such as acetic acid
Generation, the beneficially expression of albumen.Current existing report focuses mostly in shaking flask level, and has no and carry out 4-with glycerol for carbon source
The fermentation culture of hydroxyisoleucine, reduces temperature and can reduce thalline specific growth rate, beneficially genes of interest after induction
The biological activity of enzyme molecule is ensured after expression.Stream glycerol adding is cultivated, and cell yield is significantly higher than glucose, and beneficially mesh
The abduction delivering of gene, enzyme total amount is high, and economic benefit is obviously improved.One time fermentation can meet the catalysis of 3 batches.2, enzyme
Convert: more conventional test is compared, and the enzyme catalysis cycle is short, and in 4h, enzyme reaction terminates, save the production time, Conventional catalytic conversion ratio one
As about 85%, have more isoleucine remaining, be unfavorable for subsequent extracted purification, and cause substrate to waste, have a strong impact on through
Ji benefit.The present invention adds enough α-ketoglutaric acid, relatively art methods and compares, and reaction rate and conversion ratio substantially carry
High.On the one hand the α-ketoglutaric acid of excess meets thalline own metabolism system consumption, simultaneously facilitates the carrying out of this enzymatic reaction.With
Time reduce enzymatic reaction temperature, increase Mg2+Strengthen enzyme activity, reduce iron ion content and alleviate follow-up extraction pressure.3, extract:
In prior art studying extraction scheme and mainly start with from plant extract, the extraction to catalyst system and catalyzing relates to the fewest.Warp
After crossing 122 acidulous cation resin desalinations, 732 resin absorption amounts can be increased substantially, and decrease subsequent cell separation difficulty
Degree.After recrystallization in the ethanol of 75%, product liquid phase purity is obviously improved, up to more than 98%.
Detailed description of the invention
A kind of microbial enzyme conversion method produces the method for L-4-hydroxyisoleucine, comprises the following steps:
(1), the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine will can be induced to access equipped with seed tank culture base
Seed tank in, at temperature 33 DEG C-37 DEG C, pH6.5-7.5, dissolved oxygen is cultivated to bacterium solution under conditions of controlling more than 25%
OD600 value is 12-16: obtain the Escherichia coli recombinant strain of the induced production L-4-hydroxyisoleucine of maturation;
The construction method of the described Escherichia coli recombinant strain inducing production L-4-hydroxyisoleucine is: according to Su Yunjin
In bacillus cereus YL-03, gene I/D O(i.e. encodes the gene of ILE hydroxylase) it is carried out manually modified, it is thus achieved that gene
Sequence is as shown in SEQ ID NO.1;Clone this gene, and by this gene constructed enter plasmid pET28a, obtain recombiant plasmid, will
Import e. coli bl21 after recombiant plasmid, i.e. obtain inducing the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine.
(2), the Escherichia coli recombinant strain of ripe induced production L-4-hydroxyisoleucine is accessed equipped with fermentation tank
In the fermentation tank of culture medium, carry out deep liquid ventilating fermentation cultivation;In incubation, when cultivating 5h in fermentation medium one
Secondary property puts into the IPTG(isopropyl-β-D-thiogalactoside of final concentration of 0.1-0.3mmol/L) to induce, dissolved oxygen controls
At 30-50%, pH6.5-7.0, after end sugar exhausts, stream adds glycerine water solution that mass concentration is 10-80% as carbon source, ferments
The control of Cheng Wendu two rank, before adding IPTG induction, control temperature is at 34-36 DEG C, after pending abduction delivering, adjustment temperature to 30-
Cultivate for 32 DEG C;
(3), after fermentation ends, by butterfly centrifugal machine cryogenic collector thalline, and use phosphate buffer that thalline is entered
Row washing, removes phage surface impurity, then with pure water preparation catalyst system and catalyzing: a-ketoglutaric acid 30-35g/L, isoleucine
26-30g/L, ferrous sulfate 0.5-4g/L, magnesium sulfate 0.1-1g/L, ascorbic acid 0.01-0.2g/L, add in catalyst system and catalyzing
Entering the thalline after washing, forced ventilation is catalyzed, and maintenance pH is 6.0-6.5, and catalytic temperature is 28-32 DEG C, obtains catalytic liquid;Described
The addition of thalline is: every 100ml catalyst system and catalyzing adds 0.3g thalline;
(4), that catalytic liquid first passes through ceramic membrane filter is degerming, after the ultrafilter membrane of 2-4Kd molecular weight, and takes off through activated carbon
Color, after using 122 weak-acid cation-exchange resin desalinations, 732 cationic exchange resin adsorption washing with ammonia on effluent
De-, evaporation and concentration obtains L-4-hydroxyisoleucine crude product, is then recrystallized to give L-4-hydroxyisoleucine.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
The formula stating seed tank culture base is: glucose 0.5-20g/L, yeast powder 0.5-10g/L, peptone 0.5-5g/L, ammonium sulfate
0.5-10g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.5-3g/L, vitamin B 10-20mg/L, biotin 0.5-
10mg/L, ferrous sulfate 0.5-50mg/L, manganese sulfate 0.5-50mg/L, defoamer 0-0.5ml/L, remaining is water.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
The formula stating fermentation tank culture medium is: glucose 0.5-10g/L, yeast powder 0.5-10g/L, peptone 0.5-5g/L, ammonium sulfate
0.5-10g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.5-3g/L, VB1 0.5-20mg/L, VH 0.5-10mg/L, sulphuric acid
Ferrous 0.5-50mg/L, manganese sulfate 0.5-50mg/L, Semen Maydis pulp 0.5-10mL, sodium citrate 0.5-2g/L. defoamer 0-
0.5ml/L, remaining is water.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
State catalytic liquid in step (4) and be first heated to 60-80 DEG C before ceramic membrane filter is degerming, and keep 10-40min.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
Adding the 0.1-5% that charcoal amount is material liquid volume when stating activated carbon decolorizing in step (4), temperature retention time is 30-60min.
Further improvements in methods as the present invention a kind of microbial enzyme conversion method production L-4-hydroxyisoleucine: institute
Stating the ammonia mass concentration of eluting in step (4) is 1%-3%, and elution flow rate is 1-1.5BV/h.
The further improvements in methods of L-4-hydroxyisoleucine are produced: step as the present invention a kind of microbial enzyme conversion method
Suddenly evaporation and concentration described in (4), the final concentration of 500-600g/L of 4-hydroxyisoleucine crude product after evaporation and concentration.
In the present invention, the calculation of conversion ratio is as follows:
The ILE of 1mol can be catalyzed the L-4-hydroxyisoleucine producing 1mol in theory.
In terms of the conversion situation of L-4-hydroxyisoleucine content
Conversion ratio (%)=(L-4-hydroxyisoleucine yield)/(theoretical L-4-hydroxyisoleucine content) * 100%
The content use of L-4-hydroxyisoleucine, after DNFB column front derivation, liquid phase detects.
Below in conjunction with embodiment, the present invention is further illustrated:
Embodiment 1
A kind of microbial enzyme conversion method produces the method for L-4-hydroxyisoleucine, comprises the following steps:
(1), the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine will can be induced to access equipped with seed tank culture base
Seed tank in, temperature 36 DEG C, pH7.0, the OD600 value that dissolved oxygen is cultivated under conditions of controlling more than 25% to bacterium solution is 14,
I.e. obtain the Escherichia coli recombinant strain of the induced production L-4-hydroxyisoleucine of maturation;
The present embodiment can be induced the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine obtained by purchase;Its structure
Construction method is, clones its gene order gene as shown in SEQ ID NO.1;By this gene constructed enter plasmid pET28a,
To recombiant plasmid, e. coli bl21 after recombiant plasmid, will be imported, and i.e. obtain inducing the big of production L-4-hydroxyisoleucine
Enterobacteria recombinant strain;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 20mg/L, manganese sulfate 20mg/L and froth breaking
Agent 0.4ml/L, remaining is water.
(2), ripe strain is accessed in fermentation tank, cultivate under the following conditions: ventilated by deep liquid and send out
Ferment, it is different that fermentation 5h disposable stream in fermentation medium adds the final concentration (calculating with the fermentating liquid volume) IPTG(as 0.1mmol/L
Propyl group-β-D-Thiogalactopyranoside) induce.Sweat temperature section controls, and earlier fermentation controls temperature 36
DEG C, after derivant to be added, adjusting temperature 30 DEG C cultivation, after end sugar has consumed, stream adds 50% glycerol and ferments;
Fermentation tank tank formula: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate
2.5g/L, magnesium sulfate 2g/L, VB1(vitamin B1) 10mg/L, VH (biotin) 2mg/L, ferrous sulfate 50mg/L, sulphuric acid
Manganese 50mg/L, Semen Maydis pulp 10mL, sodium citrate 1g/L. defoamer 0.5ml/L, remaining is water.
(3), fermented after, by butterfly centrifugal machine cryogenic collector thalline, and use phosphate buffer that thalline is entered
Row washing, removes phage surface impurity, then with pure water preparation catalyst system and catalyzing: a-ketoglutaric acid 30g/L, isoleucine 25g/
L, ferrous sulfate 3g/L, magnesium sulfate 1 g/L, ascorbic acid 0.1 g/L, add the thalline after washing, bacterium in catalyst system and catalyzing
The addition of body is: every 100ml catalyst system and catalyzing adds 0.3g thalline, and forced ventilation is catalyzed, and maintains pH 6.5, and catalytic temperature is 30
Degree.In 4h, substrate reactions is complete, and conversion ratio reaches 95.4%;More conventional test is compared, and this programme enzyme catalysis cycle is short, and 4h endoenzyme is anti-
Should terminate, conventional method catalytic conversion, typically about 85%, has more isoleucine remaining, is unfavorable for that subsequent extracted is pure
Change, and cause substrate to waste, affect economic benefit.
(4), catalytic liquid degerming through ceramic membrane, remove a part of pigment and albumen after the ultrafilter membrane of 3Kd molecular weight, and
Through 1% activated carbon insulation decolouring, after using 122 weak-acid cation-exchange resin desalinations, upper 732 cationic exchange resin adsorption
And with 1% ammonia eluting, evaporation and concentration (the final concentration of 500g/L of evaporation and concentration) obtains L-4-hydroxyisoleucine crude product, 75%
Ethanol in recrystallization, purity is 98.8%.
Embodiment 2
A kind of microbial enzyme conversion method produces the method for L-4-hydroxyisoleucine, comprises the following steps:
(1), the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine will can be induced to access equipped with seed tank culture base
Seed tank in, temperature 37 DEG C, pH6.5, the OD600 value that dissolved oxygen is cultivated under conditions of controlling more than 25% to bacterium solution is 12:
Obtain the Escherichia coli recombinant strain of the induced production L-4-hydroxyisoleucine of maturation;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 20mg/L, manganese sulfate 20mg/L, defoamer
0.4ml/L, remaining is water.
(2), ripe strain is accessed in fermentation tank, cultivate under the following conditions: ventilated by deep liquid and send out
Ferment, fermentation 5h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.2mmol/L.
Sweat temperature section controls, and earlier fermentation control temperature, at 36 DEG C, after derivant to be added, adjusts temperature 30 DEG C cultivation,
After end sugar has consumed, stream adds 60% glycerol and ferments;
Fermentation tank tank formula: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate
2.5g/L, magnesium sulfate 2g/L, VB1 10mg/L, VH 2mg/L, ferrous sulfate 50mg/L, manganese sulfate 50mg/L, Semen Maydis pulp 10
ML, sodium citrate 1g/L, defoamer 0.5ml/L, remaining is water.
(3), fermented after, by butterfly centrifugal machine cryogenic collector thalline, and use phosphate buffer that thalline is entered
Row washing, removes phage surface impurity, then prepares catalyst system and catalyzing with pure water: add a-ketoglutaric acid 30g/L, isoleucine
26g/L, ferrous sulfate 3g/L, magnesium sulfate 0.5g/L, ascorbic acid 0.05 g/L, forced ventilation is catalyzed, and maintains pH 6.5, urges
Changing temperature is 30 degree.In 4h, substrate reactions is complete, and it is 27.86 g/L that L-4-hydroxyisoleucine produces acid, and theoretical L-4-hydroxyl is different
It is 29.2 g/L that leucine produces acid, and conversion ratio reaches 95.4%, and more conventional test is compared, and the enzyme catalysis cycle is short, enzyme reaction knot in 4h
Bundle, method catalytic conversion, about 85%, has more isoleucine remaining, is unfavorable for subsequent extracted purification, and causes substrate
Waste, affects economic benefit.
(4), catalytic liquid degerming through ceramic membrane, remove a part of pigment and albumen after the ultrafilter membrane of 3Kd molecular weight, and
Through 1% activated carbon insulation decolouring, after using 122 weak-acid cation-exchange resin desalinations, upper 732 cationic exchange resin adsorption
And with 1% ammonia eluting, evaporation and concentration (the final concentration of 600g/L of evaporation and concentration) obtains L-4-hydroxyisoleucine crude product, 75%
Ethanol in recrystallization, purity is 98.6%.
Embodiment 3
A kind of microbial enzyme conversion method produces the method for L-4-hydroxyisoleucine, comprises the following steps:
(1), the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine will can be induced to access equipped with seed tank culture base
Seed tank in, temperature 36 DEG C, pH6.8, the OD600 value that dissolved oxygen is cultivated under conditions of controlling more than 25% to bacterium solution is 13:
Obtain the Escherichia coli recombinant strain of the induced production L-4-hydroxyisoleucine of maturation;
Seed tank culture based formulas: glucose 10g/L, yeast powder 8g/L, peptone 3g/L, ammonium sulfate 4g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 1g/L, magnesium sulfate 2g/L, VB1 15mg/L, VH 5mg/L, ferrous sulfate 30mg/L, manganese sulfate 30mg/L, defoamer
0.4ml/L, remaining is water.
(2), ripe strain is accessed in fermentation tank, cultivate under the following conditions: ventilated by deep liquid and send out
Ferment, fermentation 5h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.2mmol/L.
Sweat temperature section controls, and earlier fermentation control temperature, at 35 DEG C, after derivant to be added, adjusts temperature 31 DEG C cultivation,
After end sugar has consumed, stream adds 80% glycerol and ferments;
Fermentation tank tank formula: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate
2.5g/L, magnesium sulfate 2g/L, VB1 10mg/L, VH 2mg/L, ferrous sulfate 50mg/L, manganese sulfate 50mg/L, Semen Maydis pulp 10
ML, sodium citrate 1g/L, defoamer 0.5ml/L, remaining is water.
(3), fermented after, by butterfly centrifugal machine cryogenic collector thalline, and use phosphate buffer that thalline is entered
Row washing, removes phage surface impurity, then with pure water preparation catalyst system and catalyzing: a-ketoglutaric acid 32g/L, isoleucine 28g/
L, ferrous sulfate 2g/L, magnesium sulfate 0.5g/L, ascorbic acid 0.05 g/L, add the thalline after washing in catalyst system and catalyzing,
The addition of thalline is: every 100ml catalyst system and catalyzing adds 0.3g thalline, and forced ventilation is catalyzed, and maintains pH 6.2, and catalytic temperature is
28℃.In 4h, substrate reactions is complete, and conversion ratio reaches 96.4%
(4), catalytic liquid degerming through ceramic membrane, remove a part of pigment and albumen after the ultrafilter membrane of 3Kd molecular weight, and
Through 1% activated carbon insulation decolouring, after using 122 weak-acid cation-exchange resin desalinations, upper 732 cationic exchange resin adsorption
And with 1% ammonia eluting, evaporation and concentration (the final concentration of 500g/L of evaporation and concentration) obtains L-4-hydroxyisoleucine crude product, 75%
Ethanol in recrystallization, purity is 98.3%.
The above, be only presently preferred embodiments of the present invention, and the present invention not makees any pro forma restriction, though
So the present invention is disclosed above with preferred embodiment, but is not limited to the present invention, any technology people being familiar with this specialty
Member, in the range of without departing from technical solution of the present invention, when the technology contents of available the disclosure above makes a little change or modification
For the Equivalent embodiments of equivalent variations, as long as being without departing from technical solution of the present invention content, according to the technical spirit pair of the present invention
Any simple modification, equivalent variations and the modification that above example is made, all still falls within the range of technical solution of the present invention.
Relevant comparative's test example:
It is different that this test uses the method in method of the prior art and the present invention to carry out fermenting and producing L-4-hydroxyl respectively
Leucine, then contrasts the fermentation results of each group.Strain employed in fermentation is " can induce production L-4-hydroxyl
The Escherichia coli recombinant strain of isoleucine ", the construction method of this bacterium is: clone its gene order as shown in SEQ ID NO.1
Gene;By this gene constructed enter plasmid pET28a, obtain recombiant plasmid, will after recombiant plasmid import e. coli bl21, i.e.
Obtain inducing the Escherichia coli recombinant strain producing L-4-hydroxyisoleucine;”;By each to art methods and the inventive method
Divide two groups, i.e. art methods A group, art methods B group, the inventive method A group and the inventive method B group.The most each
The method operating condition of group is as follows:
Art methods A group:
Strain is accessed in seed tank, cultivate under the following conditions: cultivation temperature 36 DEG C, pH7.0, dissolved oxygen controls
More than 25%, during subcultivation, OD600 value is 14, obtains the strain of maturation;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 10mg/L, manganese sulfate 10mg/L, defoamer
0.4ml/L, surplus is water.
Ripe strain is accessed in fermentation tank, cultivates under the following conditions: by deep liquid ventilating fermentation, send out
Ferment 5h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.1mmol/L.Fermented
Journey, ferment control temperature is at 36 DEG C, after end sugar has consumed, flows liquid feeding sugar fermentation;
Fermentation medium: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 2g/
L, magnesium sulfate 2g/L, VB1 2mg/L, VH 1mg/L, ferrous sulfate 10mg/L, manganese sulfate 5mg/L, defoamer 0.3ml/L, surplus
For water..When fermentation period about 21h is the highest, OD600 value is 35.
After having fermented, by butterfly centrifugal machine cryogenic collector thalline, and add brine, thalline is carried out pre-
Process.After 30 degree of incubations process, add a-ketoglutaric acid 28g/L, isoleucine 25g/L, ferrous sulfate 5g/L, ascorbic acid
0.02 g/L, forced ventilation is catalyzed, and maintains pH 7, and catalytic temperature is 32 degree.In 18h, substrate reactions is complete, and L-4-hydroxyl is different bright
It is 24.3g/L that propylhomoserin produces acid, and it is that 28.05 g/L conversion ratios reach 86.6% that theoretical L-4-hydroxyisoleucine produces acid;
Catalytic liquid is degerming through ceramic membrane, and through 1% activated carbon insulation decolouring, the ethanol precipitation of 90% after evaporation, upper 732 sun
Ion exchange resin adsorbs and uses 1% ammonia eluting, resin absorption amount 30g/L, evaporation and concentration (the final concentration of 500g/ of evaporation and concentration
L) obtaining L-4-hydroxyisoleucine crude product, the 4-hydroxyisoleucine content obtained is up to 40%.Product yield 40.4%.
Art methods B group:
During just strain accesses seed tank, cultivating under the following conditions: cultivation temperature 37 DEG C, pH7.0, dissolved oxygen controls
More than 25%, during subcultivation, OD600 value is 15, obtains the strain of maturation;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 20mg/L, manganese sulfate 10mg/L, defoamer
0.4ml/L, surplus is water.
Ripe strain is accessed in fermentation tank, cultivates under the following conditions: by deep liquid ventilating fermentation, send out
Ferment 4h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.1mmol/L.Fermented
Journey, ferment control temperature is at 36 DEG C, after end sugar has consumed, flows liquid feeding sugar fermentation;
Fermentation medium: glucose 7g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 2g/
L, magnesium sulfate 2g/L, VB1 2mg/L, VH 1mg/L, ferrous sulfate 10mg/L, manganese sulfate 5mg/L, defoamer 0.3ml/L, surplus
For water.Fermentation period about 21h the highest OD600 value is 40.
After having fermented, by butterfly centrifugal machine cryogenic collector thalline, and add carbonate buffer solution washing, thalline is entered
Row pretreatment.After 25 degree of incubations process, add a-ketoglutaric acid 23g/L, isoleucine 20g/L, ferrous sulfate 3g/L, anti-
Bad hematic acid 0.05 g/L, forced ventilation is catalyzed, and maintains pH 7, and catalytic temperature is 32 degree.In 16h, substrate reactions is complete, L-4-hydroxyl
It is 19.1g/L that base isoleucine produces acid, and it is 22.4 g/L that theoretical L-4-hydroxyisoleucine produces acid, and conversion ratio reaches 85.3%.
Catalytic liquid is degerming through ceramic membrane, and through 0.5% activated carbon insulation decolouring, upper 732 cationic exchange resin adsorption are also
By 2% ammonia eluting, resin absorption amount 25g/L, evaporation and concentration, obtaining L-4-hydroxyisoleucine crude product, the 4-hydroxyl obtained is different
Leucine content is up to 35%.Purifying crude product with silica gel column chromatography, purity is up to 80.1%.Product yield 32.6%.
The inventive method A group:
(1), strain is accessed in seed tank, cultivate under the following conditions: cultivation temperature 36 DEG C, pH7.0, dissolved oxygen control
System is more than 25%, and during subcultivation, OD600 value is 14, obtains the strain of maturation;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 20mg/L, manganese sulfate 20mg/L, defoamer
0.4ml/L, surplus is water.
(2), ripe strain is accessed in fermentation tank, cultivate under the following conditions: ventilated by deep liquid and send out
Ferment, fermentation 5h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.1mmol/L.
Sweat temperature section controls, and earlier fermentation control temperature, at 36 DEG C, after derivant to be added, adjusts temperature 30 DEG C cultivation,
After end sugar has consumed, stream adds 50% glycerol and ferments;
Fermentation tank tank formula: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate
2.5g/L, magnesium sulfate 2g/L, VB1 10mg/L, VH 2mg/L, ferrous sulfate 50mg/L, manganese sulfate 50mg/L, Semen Maydis pulp 10mL,
Sodium citrate 1g/L, defoamer 0.5ml/L, surplus is water.Under 21h, tank, thalline the highest OD600 value are 65.
(3), fermented after, by butterfly centrifugal machine cryogenic collector thalline, and add phosphate buffer washing, to bacterium
Body carries out pretreatment.Use phosphate buffer is buffer system, add a-ketoglutaric acid 30g/L, isoleucine 25g/L,
Ferrous sulfate 3g/L, magnesium sulfate 1 g/L, ascorbic acid 0.1 g/L, forced ventilation is catalyzed, and maintains pH 6.5, and catalytic temperature is
30 degree.In 4h, substrate reactions is complete, and conversion ratio reaches 95.4%;
(4), catalytic liquid degerming through ceramic membrane, remove a part of pigment and albumen after the ultrafilter membrane of 3Kd molecular weight, and
Through 1% activated carbon insulation decolouring, after using 122 weak-acid cation-exchange resin desalinations, upper 732 cationic exchange resin adsorption
And with 1% ammonia eluting, evaporation and concentration (the final concentration of 500g/L of evaporation and concentration) obtains L-4-hydroxyisoleucine crude product, 75%
Ethanol in recrystallization, purity is 98.8%.Product yield 60.5%.
B group of the present invention:
(1), strain is accessed in seed tank, cultivate under the following conditions: cultivation temperature 36 DEG C, pH7.0, dissolved oxygen control
System is more than 25%, and during subcultivation, OD600 value is 15, obtains the strain of maturation;
Seed tank culture based formulas: glucose 20g/L, yeast powder 10g/L, peptone 5g/L, ammonium sulfate 2g/L, phosphoric acid
Potassium dihydrogen 2g/L, magnesium sulfate 1 g/L, VB1 10mg/L, VH 0.5mg/L, ferrous sulfate 20mg/L, manganese sulfate 20mg/L, froth breaking
Agent 0.4ml/L, surplus is water.
(2), ripe strain is accessed in fermentation tank, cultivate under the following conditions: ventilated by deep liquid and send out
Ferment, fermentation 5h disposable stream in fermentation medium adds IPTG(isopropyl-beta D-thio galactopyranoside) 0.2mmol/L.
Sweat temperature section controls, and earlier fermentation control temperature, at 36 DEG C, after derivant to be added, adjusts temperature 30 DEG C cultivation,
After end sugar has consumed, stream adds 40% glycerol and ferments;Under 21h, tank, thalline the highest OD600 value are 70.
Fermentation tank tank formula: glucose 5g/L, yeast powder 5g/L, peptone 5g/L, ammonium sulfate 3g/L, potassium dihydrogen phosphate
2.5g/L, magnesium sulfate 2g/L, VB1 10mg/L, VH 2mg/L, ferrous sulfate 50mg/L, manganese sulfate 50mg/L, Semen Maydis pulp 10
ML, sodium citrate 1g/L, defoamer 0.5ml/L, surplus is water.
(3), fermented after, by butterfly centrifugal machine cryogenic collector thalline, and add phosphate buffer washing, to bacterium
Body carries out pretreatment.Use phosphate buffer is buffer system, adds a-ketoglutaric acid 30g/L, isoleucine
26g/L, ferrous sulfate 3g/L, magnesium sulfate 0.5g/L, ascorbic acid 0.05 g/L, forced ventilation is catalyzed, and maintains pH 6.5, urges
Changing temperature is 30 degree.In 4h, substrate reactions is complete, and conversion ratio reaches 95.6%;
(4), catalytic liquid degerming through ceramic membrane, remove a part of pigment and albumen after the ultrafilter membrane of 3Kd molecular weight, and
Through 1% activated carbon insulation decolouring, after using 122 weak-acid cation-exchange resin desalinations, upper 732 cationic exchange resin adsorption
And with 1% ammonia eluting, evaporation and concentration (the final concentration of 600g/L of evaporation and concentration) obtains L-4-hydroxyisoleucine crude product, 75%
Ethanol in recrystallization, purity is 98.6%.Product yield 62.4%.
Contrast and experiment is as follows:
Comparative test result shows, the inventive method can make conversion ratio be up to more than 95.4%, and product purity is up to 98%
Above, significantly improve production capacity and the efficiency of L-4-hydroxyisoleucine, there is preferable application prospect.
SEQUENCE LISTING
<110>Henan Julong Bio-Engineering Co., Ltd.
<120>a kind of method that microbial enzyme conversion method produces L-4-hydroxyisoleucine
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1875
<212> DNA
<213>artificial sequence
<400> 1
aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc 60
cgggaacgta ttcaccgtgg cattctgatc cacattacta gcgattccga cttcatggag 120
tcgagttgca gactccaatc cggactacga cgcactttat gaggtccgct tgctctcgcg 180
aggtcgcttc tctttgtatg cgccattgta gcacgtgtgt agccctgtcg taagggccat 240
gatgacttga cgtcatcccc accttcctcc agtttatcac tggcagtctc ctttgagttc 300
ccggccggac cgctggcaac aaaggataag ggttgcgctc gttgcgggac ttaacccaac 360
atttcacaac acgagctgac gacagccatg cagcacctgt ctcacggttc ccgaaggcac 420
attctcatct ctgaaaactt ccgtggatgt caagaccagg taaggttctt cgcgttgcat 480
cgaattaaac cacatgctcc accgcttgtg cgggcccccg tcaattcatt tgagttttaa 540
ccttgcggcc gtactcccca ggcggtcgac ttaacgcgtt agctccggaa gccacgcctc 600
aagggcacaa cctccaagtc gacatcgttt acggcgtgga ctaccagggt atctaatcct 660
gtttgctccc cacgctttcg cacctgagcg tcagtcttcg tccagggggc cgccttcgcc 720
accggtattc ctccagatct ctacgcattt caccgctaca cctggaattc tacccccctc 780
tacgagactc aagcttgcca gtatcagatg cagttcccag gttgagcccg gggatttcac 840
atctgactta acaaaccgcc tgcgtgcgct ttacgcccag taattccgat taacgcttgc 900
accctccgta ttaccgcggc tgctggcacg gagttagccg gtgcttcttc tgcgggtaac 960
gtcaatgagc aaaggtatta actttactcc cttcctcccc gctgaaagta ctttacaacc 1020
cgaaggcctt cttcatacac gcgggcatgg gctgcatcag gcttgcgccc attgtgcaat 1080
attccccact gctgcctccc gtaggagtct ggaccgtgtc tcagttccag tgtggctggt 1140
catcctctca gaatgaaaat gagtgggttt agcatagaag aaaaggtaca tgaatttgaa 1200
tctaaagggt ttcttgaaat ctcaaatgaa atctttttac aagaggaaga gaatcatagt 1260
ttattaacac aagcacagtt agattattat aatttggaag atgatgcgta cggtgaatgc 1320
cgtgctagat cttattcaag gtatataaag tatgttgatt caccagatta tattttagat 1380
aatagtaatg attacttcca atctaaagaa tataactatg atgatggcgg gaaagttaga 1440
cagttcaata gcataaatga tagcttttta tgtaatcctt taattcaaaa tatcgtgcgt 1500
ttcgatactg agtttgcatt taaaacaaat ataatagata aaagtaaaga tttaattata 1560
ggcttacatc aagtaagata taaagctact aaagaaagac catcttttag ttcacctatt 1620
tggttacata aagatgatga accagtagta tttttacacc ttatgaattt aagtaataca 1680
gctatcggcg gagataattt aatagctaat tctcctcggg aaattaatca gtttataagt 1740
ttgaaggagc ctttagaaac tttagtattt ggacaaaagg tcttccatgc cgtaacgcca 1800
cttggaacag aatgtagtac ggaggctttt cgtgatattt tattagtaac attttcttat 1860
aaagaaacca aataa 1875