JP2007531527A5 - - Google Patents
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- JP2007531527A5 JP2007531527A5 JP2007506524A JP2007506524A JP2007531527A5 JP 2007531527 A5 JP2007531527 A5 JP 2007531527A5 JP 2007506524 A JP2007506524 A JP 2007506524A JP 2007506524 A JP2007506524 A JP 2007506524A JP 2007531527 A5 JP2007531527 A5 JP 2007531527A5
- Authority
- JP
- Japan
- Prior art keywords
- polymerase
- probe
- nucleic acid
- target nucleic
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000523 sample Substances 0.000 claims description 67
- 238000000034 method Methods 0.000 claims description 51
- 230000000694 effects Effects 0.000 claims description 27
- 239000002773 nucleotide Substances 0.000 claims description 23
- 125000003729 nucleotide group Chemical group 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 108020004707 nucleic acids Proteins 0.000 claims description 19
- 102000039446 nucleic acids Human genes 0.000 claims description 19
- 150000007523 nucleic acids Chemical class 0.000 claims description 19
- 230000003321 amplification Effects 0.000 claims description 14
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 14
- 230000001915 proofreading effect Effects 0.000 claims description 13
- 108060002716 Exonuclease Proteins 0.000 claims description 10
- 102000013165 exonuclease Human genes 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 108010010677 Phosphodiesterase I Proteins 0.000 claims description 8
- 208000035657 Abasia Diseases 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- 101000844752 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) DNA-binding protein 7d Proteins 0.000 claims description 4
- 230000035772 mutation Effects 0.000 claims description 4
- 241000205160 Pyrococcus Species 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims 5
- 230000007017 scission Effects 0.000 claims 5
- 108091007494 Nucleic acid- binding domains Proteins 0.000 claims 2
- 101000844753 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) DNA-binding protein 7d Proteins 0.000 claims 2
- 101000844750 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) DNA-binding protein 7e Proteins 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 2
- 241000620137 Carboxydothermus hydrogenoformans Species 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 108010017826 DNA Polymerase I Proteins 0.000 claims 1
- 241000205156 Pyrococcus furiosus Species 0.000 claims 1
- 238000002875 fluorescence polarization Methods 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 description 13
- 238000011529 RT qPCR Methods 0.000 description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 238000003753 real-time PCR Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102100037935 Polyubiquitin-C Human genes 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 102100027314 Beta-2-microglobulin Human genes 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 102000015736 beta 2-Microglobulin Human genes 0.000 description 2
- 108010081355 beta 2-Microglobulin Proteins 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 101000673456 Homo sapiens 60S acidic ribosomal protein P0 Proteins 0.000 description 1
- 101001074727 Homo sapiens Ribonucleoside-diphosphate reductase large subunit Proteins 0.000 description 1
- 101000685323 Homo sapiens Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101710189720 Porphobilinogen deaminase Proteins 0.000 description 1
- 102100034391 Porphobilinogen deaminase Human genes 0.000 description 1
- 101710170827 Porphobilinogen deaminase, chloroplastic Proteins 0.000 description 1
- 101710100896 Probable porphobilinogen deaminase Proteins 0.000 description 1
- 102100036320 Ribonucleoside-diphosphate reductase large subunit Human genes 0.000 description 1
- 108010000605 Ribosomal Proteins Proteins 0.000 description 1
- 102000002278 Ribosomal Proteins Human genes 0.000 description 1
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 102100023155 Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Human genes 0.000 description 1
- 102000006467 TATA-Box Binding Protein Human genes 0.000 description 1
- 108010044281 TATA-Box Binding Protein Proteins 0.000 description 1
- 102100040296 TATA-box-binding protein Human genes 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 108010056354 Ubiquitin C Proteins 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 231100000221 frame shift mutation induction Toxicity 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 102000052035 human RPLP0 Human genes 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- CXVGEDCSTKKODG-UHFFFAOYSA-N sulisobenzone Chemical compound C1=C(S(O)(=O)=O)C(OC)=CC(O)=C1C(=O)C1=CC=CC=C1 CXVGEDCSTKKODG-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55913704P | 2004-04-01 | 2004-04-01 | |
| US60/559,137 | 2004-04-01 | ||
| PCT/US2005/010782 WO2005098042A2 (en) | 2004-04-01 | 2005-03-31 | Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007531527A JP2007531527A (ja) | 2007-11-08 |
| JP2007531527A5 true JP2007531527A5 (enExample) | 2008-02-07 |
| JP4972541B2 JP4972541B2 (ja) | 2012-07-11 |
Family
ID=35125684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007506524A Expired - Fee Related JP4972541B2 (ja) | 2004-04-01 | 2005-03-31 | 標識されたプローブおよび３’→５’エキソヌクレアーゼ活性を用いた定量的増幅方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US7445898B2 (enExample) |
| EP (1) | EP1740711A2 (enExample) |
| JP (1) | JP4972541B2 (enExample) |
| AU (1) | AU2005230833B2 (enExample) |
| CA (1) | CA2560945C (enExample) |
| WO (1) | WO2005098042A2 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
| US7793233B1 (en) | 2003-03-12 | 2010-09-07 | Microsoft Corporation | System and method for customizing note flags |
| EP1836319B1 (en) * | 2005-01-06 | 2012-09-19 | Life Technologies Corporation | Polypeptides having nucleic acid binding activity and methods for nucleic acid amplification |
| US20070059713A1 (en) | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
| US7902345B2 (en) * | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US8133701B2 (en) * | 2006-12-05 | 2012-03-13 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| JP2008182920A (ja) * | 2007-01-29 | 2008-08-14 | Sony Corp | 蛍光標識オリゴヌクレオチドと該オリゴヌクレオチドを利用する方法 |
| WO2009032781A2 (en) | 2007-08-29 | 2009-03-12 | Sequenom, Inc. | Methods and compositions for universal size-specific polymerase chain reaction |
| US20100143901A1 (en) * | 2008-12-09 | 2010-06-10 | Roche Molecular Systems, Inc. | Nuclease-Free Real-Time Detection of Nucleic Acids |
| JP5818688B2 (ja) | 2009-01-08 | 2015-11-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 核酸増幅反応の効率を改善するための方法および組成物 |
| US9778188B2 (en) | 2009-03-11 | 2017-10-03 | Industrial Technology Research Institute | Apparatus and method for detection and discrimination molecular object |
| CN102428191A (zh) * | 2009-03-18 | 2012-04-25 | 塞昆纳姆股份有限公司 | 热稳定性内切核酸酶在产生报道分子中的应用 |
| US9758817B2 (en) * | 2010-01-13 | 2017-09-12 | Agilent Technologies, Inc. | Method for identifying a nucleic acid in a sample |
| US9482615B2 (en) | 2010-03-15 | 2016-11-01 | Industrial Technology Research Institute | Single-molecule detection system and methods |
| US9670243B2 (en) | 2010-06-02 | 2017-06-06 | Industrial Technology Research Institute | Compositions and methods for sequencing nucleic acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| US9617588B2 (en) | 2010-10-05 | 2017-04-11 | Thermo Fisher Scientific Baltics Uab | Enzyme mixture |
| US8916352B2 (en) | 2011-04-08 | 2014-12-23 | Bio-Rad Laboratories, Inc. | PCR reaction mixtures with decreased non-specific activity |
| WO2013049613A1 (en) * | 2011-09-29 | 2013-04-04 | Luminex Corporation | Hydrolysis probes |
| EP4361606A3 (en) | 2012-02-03 | 2024-07-10 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| CN108753932B (zh) | 2012-08-03 | 2022-12-02 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| ITTO20121154A1 (it) * | 2012-12-27 | 2014-06-28 | Fond Istituto Italiano Di Tecnologia | Sistema di sonde per rivelare una sequenza nucleotidica bersaglio a singolo filamento |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| US11066698B2 (en) | 2015-02-17 | 2021-07-20 | Bio-Rad Laboratories, Inc. | Small nucleic acid quantification using split cycle amplification |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| CN110050071A (zh) | 2016-12-09 | 2019-07-23 | 乌尔蒂维尤股份有限公司 | 用于使用标记的核酸成像剂进行多路复用成像的改进方法 |
| WO2018155427A1 (ja) * | 2017-02-22 | 2018-08-30 | 株式会社ヨコオ | 偽陽性抑制機能を備えたプローブ、その設計方法及びその利用 |
| EP3781710A4 (en) | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| KR20210104030A (ko) | 2018-12-14 | 2021-08-24 | 얼티뷰, 인크. | 표적을 순차적으로 검출하는 방법 및 조성물 |
| CN110724729A (zh) * | 2019-11-18 | 2020-01-24 | 中国科学院成都生物研究所 | 一种荧光探针 |
| EP4114972A1 (en) * | 2020-03-06 | 2023-01-11 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
| CN111518873B (zh) * | 2020-05-11 | 2024-07-09 | 上海羿鸣生物科技有限公司 | 一种优化的扩增目标核酸的方法及应用 |
| US20210381067A1 (en) * | 2020-06-08 | 2021-12-09 | Ampliwise Inc. | Nucleic acid amplification and detection with attenuaiting probe |
| WO2022155397A1 (en) * | 2021-01-15 | 2022-07-21 | Nuprobe Usa, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
| CN118043477A (zh) * | 2021-07-09 | 2024-05-14 | 南洋理工大学 | 一种通过等温扩增检测靶标核酸的方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
| US4851331A (en) | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
| US5521301A (en) | 1988-12-12 | 1996-05-28 | City Of Hope | Genotyping of multiple allele systems |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| WO1990012115A1 (en) * | 1989-03-21 | 1990-10-18 | Collaborative Research, Inc. | A dna diagnostic test using an exonuclease activity |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5545552A (en) | 1990-12-03 | 1996-08-13 | Stratagene | Purified thermostable pyrococcus furiosus DNA polymerase I |
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| US5759772A (en) | 1992-07-23 | 1998-06-02 | Wisconsin Alumni Research Foundation | Method for determining the sex of an embryo |
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| US6120992A (en) | 1993-11-04 | 2000-09-19 | Valigene Corporation | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human |
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| EP0768976B1 (de) | 1995-05-01 | 2000-01-19 | Crown Cork AG | Schnappverschlussanordnung an einem behälter |
| US5736626A (en) | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
| US5955268A (en) | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| EP0921196A1 (en) * | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| ES2290979T3 (es) | 1997-12-15 | 2008-02-16 | Csl Behring Gmbh | Cebador marcado para uso en la deteccion de acidos nucleicos diana. |
| US6391551B1 (en) | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
| US6277578B1 (en) | 1998-03-13 | 2001-08-21 | Promega Corporation | Deploymerization method for nucleic acid detection of an amplified nucleic acid target |
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| US6355435B1 (en) * | 1999-09-10 | 2002-03-12 | Board Of Trustees Of Michigan State University | Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains |
| US6528254B1 (en) | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
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| WO2002063049A2 (en) | 2001-02-02 | 2002-08-15 | Genome Therapeutics Corporation | Methods for determining a nucleotide at a specific location within a nucleic acid molecule |
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| US20030036073A1 (en) | 2001-06-07 | 2003-02-20 | Saba James Anthony | Matrix Sequencing: a novel method of polynucleotide analysis utilizing probes containing universal nucleotides |
| EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| US7163790B2 (en) * | 2001-11-28 | 2007-01-16 | Bio-Rad Laboratories, Inc. | Parallel polymorphism scoring by amplification and error correction |
| US20030180741A1 (en) * | 2001-12-21 | 2003-09-25 | Holly Hogrefe | High fidelity DNA polymerase compositions and uses therefor |
| US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| CN1506471A (zh) * | 2002-12-09 | 2004-06-23 | 核酸检测方法 |
-
2005
- 2005-03-31 EP EP05732357A patent/EP1740711A2/en not_active Withdrawn
- 2005-03-31 WO PCT/US2005/010782 patent/WO2005098042A2/en not_active Ceased
- 2005-03-31 AU AU2005230833A patent/AU2005230833B2/en not_active Expired
- 2005-03-31 JP JP2007506524A patent/JP4972541B2/ja not_active Expired - Fee Related
- 2005-03-31 CA CA2560945A patent/CA2560945C/en not_active Expired - Lifetime
- 2005-03-31 US US11/097,463 patent/US7445898B2/en not_active Expired - Lifetime
-
2008
- 2008-11-03 US US12/264,200 patent/US8426132B2/en not_active Expired - Fee Related
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