CA2560945C - Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity - Google Patents

Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity Download PDF

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Publication number
CA2560945C
CA2560945C CA2560945A CA2560945A CA2560945C CA 2560945 C CA2560945 C CA 2560945C CA 2560945 A CA2560945 A CA 2560945A CA 2560945 A CA2560945 A CA 2560945A CA 2560945 C CA2560945 C CA 2560945C
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Canada
Prior art keywords
polymerase
probe
nucleic acid
glu
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA2560945A
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English (en)
French (fr)
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CA2560945A1 (en
Inventor
Bin Li
Lei Xi
Yan Wang
Peter B. Vander Horn
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2560945A 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity Expired - Lifetime CA2560945C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55913704P 2004-04-01 2004-04-01
US60/559,137 2004-04-01
PCT/US2005/010782 WO2005098042A2 (en) 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity

Publications (2)

Publication Number Publication Date
CA2560945A1 CA2560945A1 (en) 2005-10-20
CA2560945C true CA2560945C (en) 2013-06-18

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Family Applications (1)

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CA2560945A Expired - Lifetime CA2560945C (en) 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity

Country Status (6)

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US (2) US7445898B2 (enExample)
EP (1) EP1740711A2 (enExample)
JP (1) JP4972541B2 (enExample)
AU (1) AU2005230833B2 (enExample)
CA (1) CA2560945C (enExample)
WO (1) WO2005098042A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134686A1 (en) * 1999-10-29 2007-06-14 Stratagene California Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap
US7793233B1 (en) 2003-03-12 2010-09-07 Microsoft Corporation System and method for customizing note flags
EP1836319B1 (en) * 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptides having nucleic acid binding activity and methods for nucleic acid amplification
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7902345B2 (en) * 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US8133701B2 (en) * 2006-12-05 2012-03-13 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
JP2008182920A (ja) * 2007-01-29 2008-08-14 Sony Corp 蛍光標識オリゴヌクレオチドと該オリゴヌクレオチドを利用する方法
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US20100143901A1 (en) * 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids
JP5818688B2 (ja) 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
CN102428191A (zh) * 2009-03-18 2012-04-25 塞昆纳姆股份有限公司 热稳定性内切核酸酶在产生报道分子中的应用
US9758817B2 (en) * 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
US8916352B2 (en) 2011-04-08 2014-12-23 Bio-Rad Laboratories, Inc. PCR reaction mixtures with decreased non-specific activity
WO2013049613A1 (en) * 2011-09-29 2013-04-04 Luminex Corporation Hydrolysis probes
EP4361606A3 (en) 2012-02-03 2024-07-10 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
CN108753932B (zh) 2012-08-03 2022-12-02 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
ITTO20121154A1 (it) * 2012-12-27 2014-06-28 Fond Istituto Italiano Di Tecnologia Sistema di sonde per rivelare una sequenza nucleotidica bersaglio a singolo filamento
EP2943590A4 (en) 2013-01-13 2017-02-15 Unitaq Bio Methods and compositions for pcr using blocked and universal primers
US11066698B2 (en) 2015-02-17 2021-07-20 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
CN110050071A (zh) 2016-12-09 2019-07-23 乌尔蒂维尤股份有限公司 用于使用标记的核酸成像剂进行多路复用成像的改进方法
WO2018155427A1 (ja) * 2017-02-22 2018-08-30 株式会社ヨコオ 偽陽性抑制機能を備えたプローブ、その設計方法及びその利用
EP3781710A4 (en) 2018-04-17 2022-01-05 Chromacode, Inc. METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
KR20210104030A (ko) 2018-12-14 2021-08-24 얼티뷰, 인크. 표적을 순차적으로 검출하는 방법 및 조성물
CN110724729A (zh) * 2019-11-18 2020-01-24 中国科学院成都生物研究所 一种荧光探针
EP4114972A1 (en) * 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN111518873B (zh) * 2020-05-11 2024-07-09 上海羿鸣生物科技有限公司 一种优化的扩增目标核酸的方法及应用
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
WO2022155397A1 (en) * 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
CN118043477A (zh) * 2021-07-09 2024-05-14 南洋理工大学 一种通过等温扩增检测靶标核酸的方法

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4851331A (en) 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US5521301A (en) 1988-12-12 1996-05-28 City Of Hope Genotyping of multiple allele systems
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
WO1990012115A1 (en) * 1989-03-21 1990-10-18 Collaborative Research, Inc. A dna diagnostic test using an exonuclease activity
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5759772A (en) 1992-07-23 1998-06-02 Wisconsin Alumni Research Foundation Method for determining the sex of an embryo
US6511845B1 (en) 1992-08-07 2003-01-28 Alan R. Davis Methods for producing an immune response against HIV-1
US6120992A (en) 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5851770A (en) 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
EP0768976B1 (de) 1995-05-01 2000-01-19 Crown Cork AG Schnappverschlussanordnung an einem behälter
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US5955268A (en) 1996-04-26 1999-09-21 Abbott Laboratories Method and reagent for detecting multiple nucleic acid sequences in a test sample
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
EP0921196A1 (en) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
ES2290979T3 (es) 1997-12-15 2008-02-16 Csl Behring Gmbh Cebador marcado para uso en la deteccion de acidos nucleicos diana.
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6150105A (en) 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
US20030049657A1 (en) * 1998-11-13 2003-03-13 Cherry Joshua L. Use of primers containing non-replicatable residues for improved cycle-sequencing of nucleic acids
US6316200B1 (en) * 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
US6355435B1 (en) * 1999-09-10 2002-03-12 Board Of Trustees Of Michigan State University Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US6511810B2 (en) 2000-07-03 2003-01-28 Applera Corporation Polynucleotide sequence assay
DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
WO2002063049A2 (en) 2001-02-02 2002-08-15 Genome Therapeutics Corporation Methods for determining a nucleotide at a specific location within a nucleic acid molecule
US6720148B1 (en) 2001-02-22 2004-04-13 Caliper Life Sciences, Inc. Methods and systems for identifying nucleotides by primer extension
US20030036073A1 (en) 2001-06-07 2003-02-20 Saba James Anthony Matrix Sequencing: a novel method of polynucleotide analysis utilizing probes containing universal nucleotides
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US7163790B2 (en) * 2001-11-28 2007-01-16 Bio-Rad Laboratories, Inc. Parallel polymorphism scoring by amplification and error correction
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
CN1506471A (zh) * 2002-12-09 2004-06-23 核酸检测方法

Also Published As

Publication number Publication date
CA2560945A1 (en) 2005-10-20
WO2005098042A2 (en) 2005-10-20
JP4972541B2 (ja) 2012-07-11
US8426132B2 (en) 2013-04-23
US20100159447A1 (en) 2010-06-24
WO2005098042A3 (en) 2006-08-24
AU2005230833B2 (en) 2011-04-14
AU2005230833A1 (en) 2005-10-20
US20060024695A1 (en) 2006-02-02
EP1740711A2 (en) 2007-01-10
JP2007531527A (ja) 2007-11-08
US7445898B2 (en) 2008-11-04

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