JP2007504810A - 抗微生物剤の組換え製造 - Google Patents

抗微生物剤の組換え製造 Download PDF

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Publication number
JP2007504810A
JP2007504810A JP2006525631A JP2006525631A JP2007504810A JP 2007504810 A JP2007504810 A JP 2007504810A JP 2006525631 A JP2006525631 A JP 2006525631A JP 2006525631 A JP2006525631 A JP 2006525631A JP 2007504810 A JP2007504810 A JP 2007504810A
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JP
Japan
Prior art keywords
peptide
antimicrobial
nucleic acid
enzyme
host cell
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Ceased
Application number
JP2006525631A
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English (en)
Japanese (ja)
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JP2007504810A5 (enExample
Inventor
ベチ イェンセン，エイネア
クリステンセン ヘーゲンハウグ，ハンス−ヘンリク
カンプ ハンセン，ペテル
エリク ペデルセン，ポウル
ホルセ ミギン，ペル
Original Assignee
ノボザイムス アクティーゼルスカブ
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Application filed by ノボザイムス アクティーゼルスカブ filed Critical ノボザイムス アクティーゼルスカブ
Publication of JP2007504810A publication Critical patent/JP2007504810A/ja
Publication of JP2007504810A5 publication Critical patent/JP2007504810A5/ja
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
JP2006525631A 2003-09-11 2004-09-13 抗微生物剤の組換え製造 Ceased JP2007504810A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200301310 2003-09-11
PCT/DK2004/000605 WO2005024002A1 (en) 2003-09-11 2004-09-13 Recombinant production of antimicrobial agents

Publications (2)

Publication Number Publication Date
JP2007504810A true JP2007504810A (ja) 2007-03-08
JP2007504810A5 JP2007504810A5 (enExample) 2007-09-20

Family

ID=34259082

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006525631A Ceased JP2007504810A (ja) 2003-09-11 2004-09-13 抗微生物剤の組換え製造

Country Status (14)

Country Link
US (1) US7935501B2 (enExample)
EP (1) EP1680503B1 (enExample)
JP (1) JP2007504810A (enExample)
CN (1) CN1845989B (enExample)
AT (1) ATE513904T1 (enExample)
AU (1) AU2004270815B2 (enExample)
BR (1) BRPI0413833A (enExample)
CA (1) CA2536782A1 (enExample)
DK (1) DK1680503T3 (enExample)
ES (1) ES2368221T3 (enExample)
IL (1) IL173656A0 (enExample)
NZ (1) NZ545564A (enExample)
WO (1) WO2005024002A1 (enExample)
ZA (1) ZA200601544B (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855548B2 (en) * 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US7588926B2 (en) * 2003-02-07 2009-09-15 Novozymes A/S Proteases
US7763706B1 (en) * 2003-07-17 2010-07-27 Campina B.V. Arginine/lysine-enriched peptides
JP2007509603A (ja) 2003-10-10 2007-04-19 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
US8357408B2 (en) * 2004-06-21 2013-01-22 Novozymes A/S Proteases
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
EP2387390B1 (en) * 2009-01-13 2012-11-28 PharmaSurgics in Sweden AB Hylauronic acid containing compositions for treatment of wounds, scars, post-surgical adhesion formation
SG177704A1 (en) 2009-06-26 2012-02-28 Lysando Holding Ag Antimicrobial agents
ES2579806T3 (es) * 2009-06-26 2016-08-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Agentes antimicrobianos
CN102220295B (zh) * 2011-05-26 2013-02-06 长沙理工大学 以稻谷加工副产物为原料生产饲用葡萄糖氧化酶的方法
IN2014CN04408A (enExample) * 2011-11-17 2015-09-04 Dsm Ip Assets Bv
US10597430B2 (en) * 2012-03-05 2020-03-24 Instituto De Investigaciones Agropecuarias Chimeric gene for heterologous expression which encodes for peptides with antimicrobial activity
WO2014052438A1 (en) 2012-09-25 2014-04-03 Regents Of The University Of Minnesota Methods for making and using antimicrobial peptides
CN105734029B (zh) * 2014-12-12 2020-09-25 丰益(上海)生物技术研发中心有限公司 磷脂酶抗菌肽
CN105255939B (zh) * 2015-11-13 2018-12-25 长沙中科晶博生物科技有限公司 一种用青蒿生产菌丝霉素的方法
CN105274137B (zh) * 2015-11-13 2018-12-25 长沙中科晶博生物科技有限公司 一种用青蒿生产乳铁蛋白的方法
CN108794635B (zh) * 2018-04-04 2021-03-19 浙江工业大学 一种牛乳铁蛋白肽-人溶菌酶融合蛋白、基因及其应用
CN111118091A (zh) * 2018-10-31 2020-05-08 宁夏乙征生物工程有限公司 一种抗菌肽的生产方法
CN109295075B (zh) * 2018-10-31 2021-04-20 上海市农业生物基因中心 NfOCP1抗旱基因、其编码的氨基酸序列及其在提高植物抗旱性中的用途
CN111349177B (zh) * 2020-03-13 2022-04-22 西北农林科技大学 一种融合抗菌肽cat的制备方法和应用
CN114478795B (zh) * 2020-11-13 2024-06-04 安徽新熙盟生物科技有限公司 提高多肽类药物口服给药稳定性的融合蛋白及其应用
CN113125689B (zh) * 2021-03-29 2022-02-22 创芯国际生物科技(广州)有限公司 一种新型mtt细胞活力检测试剂盒及其应用
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114790237B (zh) * 2022-05-31 2024-04-05 合肥工业大学 一种抗菌肽及其应用
CN118064343B (zh) * 2024-03-11 2025-01-03 华南农业大学 一种产四种抗菌肽的重组枯草芽孢杆菌、构建方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10509317A (ja) * 1994-11-02 1998-09-14 エイジェニックス・インコーポレイテッド アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片
JPH11503006A (ja) * 1995-03-13 1999-03-23 ユニバーシティー オブ ブリティッシュ コロンビア カチオン性ペプチドの製造方法
JP2002502246A (ja) * 1997-05-28 2002-01-22 サムヤン ジェネックス コーポレイション 抗微生物ペプチドを大量生産するための方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5094951A (en) 1988-06-21 1992-03-10 Chiron Corporation Production of glucose oxidase in recombinant systems
WO2000075344A1 (en) 1999-06-02 2000-12-14 Novozymes A/S Pectate lyase fusion for expression and secretion of polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10509317A (ja) * 1994-11-02 1998-09-14 エイジェニックス・インコーポレイテッド アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片
JPH11503006A (ja) * 1995-03-13 1999-03-23 ユニバーシティー オブ ブリティッシュ コロンビア カチオン性ペプチドの製造方法
JP2002502246A (ja) * 1997-05-28 2002-01-22 サムヤン ジェネックス コーポレイション 抗微生物ペプチドを大量生産するための方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6010040942; Ｐｌａｎｔ　Ｃｅｌｌ　Ｐｈｙｓｉｏｌ． ｖｏｌ．３９　ｎｏ．１, 1998, ｐｐ．５７-６３ *

Also Published As

Publication number Publication date
EP1680503A1 (en) 2006-07-19
ZA200601544B (en) 2007-04-25
CA2536782A1 (en) 2005-03-17
CN1845989A (zh) 2006-10-11
WO2005024002A1 (en) 2005-03-17
NZ545564A (en) 2008-07-31
IL173656A0 (en) 2006-07-05
ES2368221T3 (es) 2011-11-15
EP1680503B1 (en) 2011-06-22
DK1680503T3 (da) 2011-10-03
CN1845989B (zh) 2011-10-12
ATE513904T1 (de) 2011-07-15
AU2004270815A1 (en) 2005-03-17
BRPI0413833A (pt) 2006-10-24
US20070104764A1 (en) 2007-05-10
AU2004270815B2 (en) 2011-06-02
US7935501B2 (en) 2011-05-03

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